Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

James J. Steinhardt, Javier Guenaga, Hannah L. Turner, Krisha McKee, Mark K. Louder, Sijy O’Dell, Chi-I Chiang, Lin Lei, Andrey Galkin, Alexander K. Andrianov, Nicole A. Doria-Rose, Robert T. Bailer, Andrew B. Ward, John R. Mascola, Yuxing Li

ABSTRACT

HIV-1 broadly neutralizing antibodies (bNAbs) are being explored as passively administered therapeutic and preventative agents. However, the extensively diversified HIV-1 envelope glycoproteins (Env) rapidly acquire mutations to evade individual bNAbs in monotherapy regimens. The use of a "single" agent to simultaneously target distinct Env epitopes is desirable to overcome viral diversity. Here, we report the use of tandem single-chain variable fragment (ScFv) domains of two bNAbs, specific for the CD4-binding site and V3 glycan patch, to form anti-HIV-1 bispecific ScFvs (Bi-ScFvs). The optimal Bi-ScFv crosslinks adjacent protomers within one HIV-1 Env spike and has greater neutralization breadth than its parental bNAbs. Furthermore, the combination of this Bi-ScFv with a third bNAb recognizing the Env membrane proximal external region (MPER) results in a trispecific bNAb, which has nearly pan-isolate neutralization breadth and high potency. Thus, multispecific antibodies combining functional moieties of bNAbs could achieve outstanding neutralization capacity with augmented avidity. More... »

PAGES

877

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-018-03335-4

DOI

http://dx.doi.org/10.1038/s41467-018-03335-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101191492

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29491415


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Neutralizing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antiviral Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Combinations", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Design", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV Antibodies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Subunits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Single-Chain Antibodies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "env Gene Products, Human Immunodeficiency Virus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Maryland, College Park", 
          "id": "https://www.grid.ac/institutes/grid.164295.d", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA", 
            "Virology Program at the University of Maryland, 20740, College Park, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Steinhardt", 
        "givenName": "James J.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "IAVI Neutralizing Antibody Center, The Scripps Research Institute, 92037, La Jolla, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guenaga", 
        "givenName": "Javier", 
        "id": "sg:person.01217453125.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217453125.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 92037, La Jolla, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Turner", 
        "givenName": "Hannah L.", 
        "id": "sg:person.01300627011.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300627011.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "McKee", 
        "givenName": "Krisha", 
        "id": "sg:person.01027662652.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027662652.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Louder", 
        "givenName": "Mark K.", 
        "id": "sg:person.01206060112.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206060112.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "O\u2019Dell", 
        "givenName": "Sijy", 
        "id": "sg:person.0700652546.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700652546.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universities at Shady Grove", 
          "id": "https://www.grid.ac/institutes/grid.440664.4", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chiang", 
        "givenName": "Chi-I", 
        "id": "sg:person.013223766455.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013223766455.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universities at Shady Grove", 
          "id": "https://www.grid.ac/institutes/grid.440664.4", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lei", 
        "givenName": "Lin", 
        "id": "sg:person.011562172017.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011562172017.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Maryland, Baltimore", 
          "id": "https://www.grid.ac/institutes/grid.411024.2", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA", 
            "Department of Microbiology and Immunology, University of Maryland School of Medicine, 21201, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Galkin", 
        "givenName": "Andrey", 
        "id": "sg:person.01250123424.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250123424.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universities at Shady Grove", 
          "id": "https://www.grid.ac/institutes/grid.440664.4", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Andrianov", 
        "givenName": "Alexander K.", 
        "id": "sg:person.0750102500.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750102500.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Doria-Rose", 
        "givenName": "Nicole A.", 
        "id": "sg:person.01134103612.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134103612.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bailer", 
        "givenName": "Robert T.", 
        "id": "sg:person.01204607222.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204607222.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 92037, La Jolla, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ward", 
        "givenName": "Andrew B.", 
        "id": "sg:person.0640074731.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640074731.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Allergy and Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.419681.3", 
          "name": [
            "Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mascola", 
        "givenName": "John R.", 
        "id": "sg:person.01270042052.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270042052.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Maryland, Baltimore", 
          "id": "https://www.grid.ac/institutes/grid.411024.2", 
          "name": [
            "Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA", 
            "Virology Program at the University of Maryland, 20740, College Park, MD, USA", 
            "Department of Microbiology and Immunology, University of Maryland School of Medicine, 21201, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yuxing", 
        "id": "sg:person.013247615607.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013247615607.85"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.virusres.2007.01.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002874422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11544", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003615458", 
          "https://doi.org/10.1038/nature11544"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcc.20084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008225564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1187659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008550585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1005520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009150169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02652-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009395374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.04.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010540851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.04.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010540851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-3991(99)00043-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011135558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02108-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011345641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni.3158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011379259", 
          "https://doi.org/10.1038/ni.3158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1415789111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012000426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1315295110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013726505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.79.16.10108-10125.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014523492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.4268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015097302", 
          "https://doi.org/10.1038/nm.4268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018512919", 
          "https://doi.org/10.1038/nri891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018512919", 
          "https://doi.org/10.1038/nri891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018849649", 
          "https://doi.org/10.1038/nbt1142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018849649", 
          "https://doi.org/10.1038/nbt1142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1255512", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021544490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2009.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021992126"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bmb/58.1.19", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023033026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.01893-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024698263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.06745-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025531035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scitranslmed.aad5752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025881110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.addr.2015.10.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026445042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/980250", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027019318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13612", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027070811", 
          "https://doi.org/10.1038/nature13612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.immuni.2016.10.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031418295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.immuni.2016.10.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031418295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1389-0344(01)00083-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032108315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.03608-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032392977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1304985110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032672940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02097-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033317263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/9.7.617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034960889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.04.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035047834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.04.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035047834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2003.08.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036377160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2003.08.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036377160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036417872", 
          "https://doi.org/10.1038/nature14264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038250514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038250514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qai.0000000000000218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039279758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qai.0000000000000218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039279758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.05.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040111915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.05.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040111915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fimmu.2014.00520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041053562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20101176", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041517979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.22.10134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042377987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.01.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043097864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.01.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043097864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2009.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043460024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1207227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044060497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044845665", 
          "https://doi.org/10.1038/nature10373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14411", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045337010", 
          "https://doi.org/10.1038/nature14411"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.addr.2012.09.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045836599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1003618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046399042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/cei.12692", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046493197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02213-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046590342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1608243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047925582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1608243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047925582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.4063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048258887", 
          "https://doi.org/10.1038/nm.4063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.106.091561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049396823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049794727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049794727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/19420862.2016.1197457", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050383068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2013.07.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050934306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2013.07.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050934306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.03136-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051387150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051453410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0000000000000154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051453410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/515697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058790085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/aid.1994.10.359", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059223893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a021436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060407063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.288.5472.1789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062569801"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.03246-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062713180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074626707", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077462666", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1006212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083856519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature21435", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084128563", 
          "https://doi.org/10.1038/nature21435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aan8630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091931726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aan8630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091931726"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "HIV-1 broadly neutralizing antibodies (bNAbs) are being explored as passively administered therapeutic and preventative agents. However, the extensively diversified HIV-1 envelope glycoproteins (Env) rapidly acquire mutations to evade individual bNAbs in monotherapy regimens. The use of a \"single\" agent to simultaneously target distinct Env epitopes is desirable to overcome viral diversity. Here, we report the use of tandem single-chain variable fragment (ScFv) domains of two bNAbs, specific for the CD4-binding site and V3 glycan patch, to form anti-HIV-1 bispecific ScFvs (Bi-ScFvs). The optimal Bi-ScFv crosslinks adjacent protomers within one HIV-1 Env spike and has greater neutralization breadth than its parental bNAbs. Furthermore, the combination of this Bi-ScFv with a third bNAb recognizing the Env membrane proximal external region (MPER) results in a trispecific bNAb, which has nearly pan-isolate neutralization breadth and high potency. Thus, multispecific antibodies combining functional moieties of bNAbs could achieve outstanding neutralization capacity with augmented avidity.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-018-03335-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5503257", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6581983", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2438697", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity", 
    "pagination": "877", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7b729f1507a5b991673e3c15141eb1ef6cf75b9afc821010b562acbe0a5b1850"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29491415"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-018-03335-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1101191492"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-018-03335-4", 
      "https://app.dimensions.ai/details/publication/pub.1101191492"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000348_0000000348/records_54298_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-018-03335-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-03335-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-03335-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-03335-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-03335-4'


 

This table displays all metadata directly associated to this object as RDF triples.

453 TRIPLES      21 PREDICATES      110 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-018-03335-4 schema:about N100de64bfa584d0ab0f6b703024d1653
2 N2074894124f84385a65975b62a6ced56
3 N2e338bff43c2433589e0d8b0fdd7ddad
4 N454b495602634acb997864e3fdd55563
5 N50a988fba01749b98618aa896ccd83df
6 N5f40f1500cfb4293870ee5fe80b85d10
7 N6d3a2c73bb3641c79f913382574f4bf2
8 N83146b230c4b4fc98a7f8554f01699b8
9 N8409b3e7648d489aae5d1c927aeed8ee
10 N86430515685b4842bd8791b676fdcd6c
11 Ncb2ef16aee3c49639468a99862455818
12 Ncb5a23f2f5db41fc8aaa12d08ca8ed9b
13 Nce76efcf753c4a25a0c1498c8361bd6d
14 Nd0fab07ea8724179a2d5c534b8978a6f
15 anzsrc-for:11
16 anzsrc-for:1108
17 schema:author N2a85b31dfb424a449916866dcae313aa
18 schema:citation sg:pub.10.1038/nature10373
19 sg:pub.10.1038/nature11544
20 sg:pub.10.1038/nature13612
21 sg:pub.10.1038/nature14264
22 sg:pub.10.1038/nature14411
23 sg:pub.10.1038/nature21435
24 sg:pub.10.1038/nbt1142
25 sg:pub.10.1038/ni.3158
26 sg:pub.10.1038/nm.4063
27 sg:pub.10.1038/nm.4268
28 sg:pub.10.1038/nri891
29 https://app.dimensions.ai/details/publication/pub.1074626707
30 https://app.dimensions.ai/details/publication/pub.1077462666
31 https://doi.org/10.1002/jcc.20084
32 https://doi.org/10.1016/j.addr.2012.09.039
33 https://doi.org/10.1016/j.addr.2015.10.022
34 https://doi.org/10.1016/j.cell.2015.01.016
35 https://doi.org/10.1016/j.cell.2016.04.010
36 https://doi.org/10.1016/j.cell.2016.04.050
37 https://doi.org/10.1016/j.cell.2016.05.024
38 https://doi.org/10.1016/j.immuni.2016.10.027
39 https://doi.org/10.1016/j.jsb.2003.08.005
40 https://doi.org/10.1016/j.jsb.2009.01.002
41 https://doi.org/10.1016/j.jsb.2009.01.004
42 https://doi.org/10.1016/j.virusres.2007.01.005
43 https://doi.org/10.1016/j.ymeth.2013.07.005
44 https://doi.org/10.1016/s0304-3991(99)00043-1
45 https://doi.org/10.1016/s1389-0344(01)00083-1
46 https://doi.org/10.1056/nejmoa1608243
47 https://doi.org/10.1073/pnas.1304985110
48 https://doi.org/10.1073/pnas.1315295110
49 https://doi.org/10.1073/pnas.1415789111
50 https://doi.org/10.1073/pnas.88.22.10134
51 https://doi.org/10.1080/19420862.2016.1197457
52 https://doi.org/10.1084/jem.20101176
53 https://doi.org/10.1086/515697
54 https://doi.org/10.1089/aid.1994.10.359
55 https://doi.org/10.1093/bmb/58.1.19
56 https://doi.org/10.1093/protein/9.7.617
57 https://doi.org/10.1097/coh.0000000000000144
58 https://doi.org/10.1097/coh.0000000000000148
59 https://doi.org/10.1097/coh.0000000000000154
60 https://doi.org/10.1097/qai.0000000000000218
61 https://doi.org/10.1101/cshperspect.a021436
62 https://doi.org/10.1111/cei.12692
63 https://doi.org/10.1126/science.1187659
64 https://doi.org/10.1126/science.1207227
65 https://doi.org/10.1126/science.1255512
66 https://doi.org/10.1126/science.288.5472.1789
67 https://doi.org/10.1126/science.aan8630
68 https://doi.org/10.1126/scitranslmed.aad5752
69 https://doi.org/10.1128/jvi.01893-12
70 https://doi.org/10.1128/jvi.02097-15
71 https://doi.org/10.1128/jvi.02108-09
72 https://doi.org/10.1128/jvi.02213-14
73 https://doi.org/10.1128/jvi.02652-15
74 https://doi.org/10.1128/jvi.03136-14
75 https://doi.org/10.1128/jvi.03246-15
76 https://doi.org/10.1128/jvi.03608-14
77 https://doi.org/10.1128/jvi.06745-11
78 https://doi.org/10.1128/jvi.79.16.10108-10125.2005
79 https://doi.org/10.1155/2012/980250
80 https://doi.org/10.1371/journal.ppat.1003618
81 https://doi.org/10.1371/journal.ppat.1005520
82 https://doi.org/10.1371/journal.ppat.1006212
83 https://doi.org/10.1529/biophysj.106.091561
84 https://doi.org/10.3389/fimmu.2014.00520
85 schema:datePublished 2018-12
86 schema:datePublishedReg 2018-12-01
87 schema:description HIV-1 broadly neutralizing antibodies (bNAbs) are being explored as passively administered therapeutic and preventative agents. However, the extensively diversified HIV-1 envelope glycoproteins (Env) rapidly acquire mutations to evade individual bNAbs in monotherapy regimens. The use of a "single" agent to simultaneously target distinct Env epitopes is desirable to overcome viral diversity. Here, we report the use of tandem single-chain variable fragment (ScFv) domains of two bNAbs, specific for the CD4-binding site and V3 glycan patch, to form anti-HIV-1 bispecific ScFvs (Bi-ScFvs). The optimal Bi-ScFv crosslinks adjacent protomers within one HIV-1 Env spike and has greater neutralization breadth than its parental bNAbs. Furthermore, the combination of this Bi-ScFv with a third bNAb recognizing the Env membrane proximal external region (MPER) results in a trispecific bNAb, which has nearly pan-isolate neutralization breadth and high potency. Thus, multispecific antibodies combining functional moieties of bNAbs could achieve outstanding neutralization capacity with augmented avidity.
88 schema:genre research_article
89 schema:inLanguage en
90 schema:isAccessibleForFree true
91 schema:isPartOf N1c62bd6f5e884af097f331665f257f3f
92 N2a458c18459048bd8789d48b2662cf64
93 sg:journal.1043282
94 schema:name Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity
95 schema:pagination 877
96 schema:productId N08f3937b65754200951fbf45992d0c51
97 N52388f5f78ca45868f5a00870d07b569
98 N7f28e249cca644be89a6df5a49ee0978
99 N9db6b71120a94385a87f31712d3536b2
100 Nef15c62ac82e4872883bca3d1d1d8157
101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101191492
102 https://doi.org/10.1038/s41467-018-03335-4
103 schema:sdDatePublished 2019-04-11T10:15
104 schema:sdLicense https://scigraph.springernature.com/explorer/license/
105 schema:sdPublisher Na7738b0e2db44994bf842532931ab34b
106 schema:url https://www.nature.com/articles/s41467-018-03335-4
107 sgo:license sg:explorer/license/
108 sgo:sdDataset articles
109 rdf:type schema:ScholarlyArticle
110 N08f3937b65754200951fbf45992d0c51 schema:name nlm_unique_id
111 schema:value 101528555
112 rdf:type schema:PropertyValue
113 N0fe87034734c452ea4ded1504520b3e6 rdf:first sg:person.01217453125.26
114 rdf:rest Nf2445ded453646ea82118edeef1f1e94
115 N100de64bfa584d0ab0f6b703024d1653 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Mutation
117 rdf:type schema:DefinedTerm
118 N1c62bd6f5e884af097f331665f257f3f schema:issueNumber 1
119 rdf:type schema:PublicationIssue
120 N1f27cbadbed94963b007010f168f30de rdf:first sg:person.0750102500.57
121 rdf:rest Na38e1c5bc56d43f2b708b70229784050
122 N2074894124f84385a65975b62a6ced56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name HIV Antibodies
124 rdf:type schema:DefinedTerm
125 N25621d3b69ca4a998332f64b54bd8930 rdf:first sg:person.01250123424.99
126 rdf:rest N1f27cbadbed94963b007010f168f30de
127 N2a458c18459048bd8789d48b2662cf64 schema:volumeNumber 9
128 rdf:type schema:PublicationVolume
129 N2a85b31dfb424a449916866dcae313aa rdf:first N755286df306741a49cc642bdef542174
130 rdf:rest N0fe87034734c452ea4ded1504520b3e6
131 N2e338bff43c2433589e0d8b0fdd7ddad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Drug Design
133 rdf:type schema:DefinedTerm
134 N30a883f864c949b9ae4d21e78646ad12 rdf:first sg:person.013223766455.67
135 rdf:rest Nd17438b7519f48449225e679b501d2e9
136 N38af89a1bd624ba1b8138ae1ac60e34f rdf:first sg:person.0640074731.29
137 rdf:rest N86982edb5cda4c77ba5de8ef8609560e
138 N43487d27cc6449358065b3832e297f73 rdf:first sg:person.01027662652.26
139 rdf:rest Nf528342404364aaa9a1686b563e46586
140 N454b495602634acb997864e3fdd55563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Humans
142 rdf:type schema:DefinedTerm
143 N4f9169b486b94703b3ba0262df98d880 rdf:first sg:person.013247615607.85
144 rdf:rest rdf:nil
145 N50a988fba01749b98618aa896ccd83df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Epitopes
147 rdf:type schema:DefinedTerm
148 N52388f5f78ca45868f5a00870d07b569 schema:name dimensions_id
149 schema:value pub.1101191492
150 rdf:type schema:PropertyValue
151 N5f40f1500cfb4293870ee5fe80b85d10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Microscopy, Electron
153 rdf:type schema:DefinedTerm
154 N6d3a2c73bb3641c79f913382574f4bf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Drug Combinations
156 rdf:type schema:DefinedTerm
157 N755286df306741a49cc642bdef542174 schema:affiliation https://www.grid.ac/institutes/grid.164295.d
158 schema:familyName Steinhardt
159 schema:givenName James J.
160 rdf:type schema:Person
161 N7f28e249cca644be89a6df5a49ee0978 schema:name doi
162 schema:value 10.1038/s41467-018-03335-4
163 rdf:type schema:PropertyValue
164 N83146b230c4b4fc98a7f8554f01699b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name HIV Infections
166 rdf:type schema:DefinedTerm
167 N8409b3e7648d489aae5d1c927aeed8ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name env Gene Products, Human Immunodeficiency Virus
169 rdf:type schema:DefinedTerm
170 N86430515685b4842bd8791b676fdcd6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Single-Chain Antibodies
172 rdf:type schema:DefinedTerm
173 N86982edb5cda4c77ba5de8ef8609560e rdf:first sg:person.01270042052.88
174 rdf:rest N4f9169b486b94703b3ba0262df98d880
175 N9db6b71120a94385a87f31712d3536b2 schema:name pubmed_id
176 schema:value 29491415
177 rdf:type schema:PropertyValue
178 Na38e1c5bc56d43f2b708b70229784050 rdf:first sg:person.01134103612.24
179 rdf:rest Nd89b68e02f854c31a6a147cdf1a58a25
180 Na7738b0e2db44994bf842532931ab34b schema:name Springer Nature - SN SciGraph project
181 rdf:type schema:Organization
182 Nc7aab9ff7e9c42468ca2d9145a9556aa rdf:first sg:person.0700652546.79
183 rdf:rest N30a883f864c949b9ae4d21e78646ad12
184 Ncb2ef16aee3c49639468a99862455818 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Antiviral Agents
186 rdf:type schema:DefinedTerm
187 Ncb5a23f2f5db41fc8aaa12d08ca8ed9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name HIV-1
189 rdf:type schema:DefinedTerm
190 Nce76efcf753c4a25a0c1498c8361bd6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Antibodies, Neutralizing
192 rdf:type schema:DefinedTerm
193 Nd0fab07ea8724179a2d5c534b8978a6f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Protein Subunits
195 rdf:type schema:DefinedTerm
196 Nd17438b7519f48449225e679b501d2e9 rdf:first sg:person.011562172017.86
197 rdf:rest N25621d3b69ca4a998332f64b54bd8930
198 Nd89b68e02f854c31a6a147cdf1a58a25 rdf:first sg:person.01204607222.81
199 rdf:rest N38af89a1bd624ba1b8138ae1ac60e34f
200 Nef15c62ac82e4872883bca3d1d1d8157 schema:name readcube_id
201 schema:value 7b729f1507a5b991673e3c15141eb1ef6cf75b9afc821010b562acbe0a5b1850
202 rdf:type schema:PropertyValue
203 Nf2445ded453646ea82118edeef1f1e94 rdf:first sg:person.01300627011.85
204 rdf:rest N43487d27cc6449358065b3832e297f73
205 Nf528342404364aaa9a1686b563e46586 rdf:first sg:person.01206060112.94
206 rdf:rest Nc7aab9ff7e9c42468ca2d9145a9556aa
207 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
208 schema:name Medical and Health Sciences
209 rdf:type schema:DefinedTerm
210 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
211 schema:name Medical Microbiology
212 rdf:type schema:DefinedTerm
213 sg:grant.2438697 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-03335-4
214 rdf:type schema:MonetaryGrant
215 sg:grant.5503257 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-03335-4
216 rdf:type schema:MonetaryGrant
217 sg:grant.6581983 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-03335-4
218 rdf:type schema:MonetaryGrant
219 sg:journal.1043282 schema:issn 2041-1723
220 schema:name Nature Communications
221 rdf:type schema:Periodical
222 sg:person.01027662652.26 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
223 schema:familyName McKee
224 schema:givenName Krisha
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027662652.26
226 rdf:type schema:Person
227 sg:person.01134103612.24 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
228 schema:familyName Doria-Rose
229 schema:givenName Nicole A.
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134103612.24
231 rdf:type schema:Person
232 sg:person.011562172017.86 schema:affiliation https://www.grid.ac/institutes/grid.440664.4
233 schema:familyName Lei
234 schema:givenName Lin
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011562172017.86
236 rdf:type schema:Person
237 sg:person.01204607222.81 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
238 schema:familyName Bailer
239 schema:givenName Robert T.
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204607222.81
241 rdf:type schema:Person
242 sg:person.01206060112.94 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
243 schema:familyName Louder
244 schema:givenName Mark K.
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206060112.94
246 rdf:type schema:Person
247 sg:person.01217453125.26 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
248 schema:familyName Guenaga
249 schema:givenName Javier
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217453125.26
251 rdf:type schema:Person
252 sg:person.01250123424.99 schema:affiliation https://www.grid.ac/institutes/grid.411024.2
253 schema:familyName Galkin
254 schema:givenName Andrey
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250123424.99
256 rdf:type schema:Person
257 sg:person.01270042052.88 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
258 schema:familyName Mascola
259 schema:givenName John R.
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270042052.88
261 rdf:type schema:Person
262 sg:person.01300627011.85 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
263 schema:familyName Turner
264 schema:givenName Hannah L.
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300627011.85
266 rdf:type schema:Person
267 sg:person.013223766455.67 schema:affiliation https://www.grid.ac/institutes/grid.440664.4
268 schema:familyName Chiang
269 schema:givenName Chi-I
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013223766455.67
271 rdf:type schema:Person
272 sg:person.013247615607.85 schema:affiliation https://www.grid.ac/institutes/grid.411024.2
273 schema:familyName Li
274 schema:givenName Yuxing
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013247615607.85
276 rdf:type schema:Person
277 sg:person.0640074731.29 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
278 schema:familyName Ward
279 schema:givenName Andrew B.
280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640074731.29
281 rdf:type schema:Person
282 sg:person.0700652546.79 schema:affiliation https://www.grid.ac/institutes/grid.419681.3
283 schema:familyName O’Dell
284 schema:givenName Sijy
285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700652546.79
286 rdf:type schema:Person
287 sg:person.0750102500.57 schema:affiliation https://www.grid.ac/institutes/grid.440664.4
288 schema:familyName Andrianov
289 schema:givenName Alexander K.
290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750102500.57
291 rdf:type schema:Person
292 sg:pub.10.1038/nature10373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044845665
293 https://doi.org/10.1038/nature10373
294 rdf:type schema:CreativeWork
295 sg:pub.10.1038/nature11544 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003615458
296 https://doi.org/10.1038/nature11544
297 rdf:type schema:CreativeWork
298 sg:pub.10.1038/nature13612 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027070811
299 https://doi.org/10.1038/nature13612
300 rdf:type schema:CreativeWork
301 sg:pub.10.1038/nature14264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036417872
302 https://doi.org/10.1038/nature14264
303 rdf:type schema:CreativeWork
304 sg:pub.10.1038/nature14411 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045337010
305 https://doi.org/10.1038/nature14411
306 rdf:type schema:CreativeWork
307 sg:pub.10.1038/nature21435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084128563
308 https://doi.org/10.1038/nature21435
309 rdf:type schema:CreativeWork
310 sg:pub.10.1038/nbt1142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018849649
311 https://doi.org/10.1038/nbt1142
312 rdf:type schema:CreativeWork
313 sg:pub.10.1038/ni.3158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011379259
314 https://doi.org/10.1038/ni.3158
315 rdf:type schema:CreativeWork
316 sg:pub.10.1038/nm.4063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048258887
317 https://doi.org/10.1038/nm.4063
318 rdf:type schema:CreativeWork
319 sg:pub.10.1038/nm.4268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015097302
320 https://doi.org/10.1038/nm.4268
321 rdf:type schema:CreativeWork
322 sg:pub.10.1038/nri891 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018512919
323 https://doi.org/10.1038/nri891
324 rdf:type schema:CreativeWork
325 https://app.dimensions.ai/details/publication/pub.1074626707 schema:CreativeWork
326 https://app.dimensions.ai/details/publication/pub.1077462666 schema:CreativeWork
327 https://doi.org/10.1002/jcc.20084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008225564
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1016/j.addr.2012.09.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045836599
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1016/j.addr.2015.10.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026445042
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1016/j.cell.2015.01.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043097864
334 rdf:type schema:CreativeWork
335 https://doi.org/10.1016/j.cell.2016.04.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010540851
336 rdf:type schema:CreativeWork
337 https://doi.org/10.1016/j.cell.2016.04.050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035047834
338 rdf:type schema:CreativeWork
339 https://doi.org/10.1016/j.cell.2016.05.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040111915
340 rdf:type schema:CreativeWork
341 https://doi.org/10.1016/j.immuni.2016.10.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031418295
342 rdf:type schema:CreativeWork
343 https://doi.org/10.1016/j.jsb.2003.08.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036377160
344 rdf:type schema:CreativeWork
345 https://doi.org/10.1016/j.jsb.2009.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043460024
346 rdf:type schema:CreativeWork
347 https://doi.org/10.1016/j.jsb.2009.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021992126
348 rdf:type schema:CreativeWork
349 https://doi.org/10.1016/j.virusres.2007.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002874422
350 rdf:type schema:CreativeWork
351 https://doi.org/10.1016/j.ymeth.2013.07.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050934306
352 rdf:type schema:CreativeWork
353 https://doi.org/10.1016/s0304-3991(99)00043-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011135558
354 rdf:type schema:CreativeWork
355 https://doi.org/10.1016/s1389-0344(01)00083-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032108315
356 rdf:type schema:CreativeWork
357 https://doi.org/10.1056/nejmoa1608243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047925582
358 rdf:type schema:CreativeWork
359 https://doi.org/10.1073/pnas.1304985110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032672940
360 rdf:type schema:CreativeWork
361 https://doi.org/10.1073/pnas.1315295110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013726505
362 rdf:type schema:CreativeWork
363 https://doi.org/10.1073/pnas.1415789111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012000426
364 rdf:type schema:CreativeWork
365 https://doi.org/10.1073/pnas.88.22.10134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042377987
366 rdf:type schema:CreativeWork
367 https://doi.org/10.1080/19420862.2016.1197457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050383068
368 rdf:type schema:CreativeWork
369 https://doi.org/10.1084/jem.20101176 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041517979
370 rdf:type schema:CreativeWork
371 https://doi.org/10.1086/515697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058790085
372 rdf:type schema:CreativeWork
373 https://doi.org/10.1089/aid.1994.10.359 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059223893
374 rdf:type schema:CreativeWork
375 https://doi.org/10.1093/bmb/58.1.19 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023033026
376 rdf:type schema:CreativeWork
377 https://doi.org/10.1093/protein/9.7.617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034960889
378 rdf:type schema:CreativeWork
379 https://doi.org/10.1097/coh.0000000000000144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038250514
380 rdf:type schema:CreativeWork
381 https://doi.org/10.1097/coh.0000000000000148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049794727
382 rdf:type schema:CreativeWork
383 https://doi.org/10.1097/coh.0000000000000154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051453410
384 rdf:type schema:CreativeWork
385 https://doi.org/10.1097/qai.0000000000000218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039279758
386 rdf:type schema:CreativeWork
387 https://doi.org/10.1101/cshperspect.a021436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060407063
388 rdf:type schema:CreativeWork
389 https://doi.org/10.1111/cei.12692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046493197
390 rdf:type schema:CreativeWork
391 https://doi.org/10.1126/science.1187659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008550585
392 rdf:type schema:CreativeWork
393 https://doi.org/10.1126/science.1207227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044060497
394 rdf:type schema:CreativeWork
395 https://doi.org/10.1126/science.1255512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021544490
396 rdf:type schema:CreativeWork
397 https://doi.org/10.1126/science.288.5472.1789 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062569801
398 rdf:type schema:CreativeWork
399 https://doi.org/10.1126/science.aan8630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091931726
400 rdf:type schema:CreativeWork
401 https://doi.org/10.1126/scitranslmed.aad5752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025881110
402 rdf:type schema:CreativeWork
403 https://doi.org/10.1128/jvi.01893-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024698263
404 rdf:type schema:CreativeWork
405 https://doi.org/10.1128/jvi.02097-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033317263
406 rdf:type schema:CreativeWork
407 https://doi.org/10.1128/jvi.02108-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011345641
408 rdf:type schema:CreativeWork
409 https://doi.org/10.1128/jvi.02213-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046590342
410 rdf:type schema:CreativeWork
411 https://doi.org/10.1128/jvi.02652-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009395374
412 rdf:type schema:CreativeWork
413 https://doi.org/10.1128/jvi.03136-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051387150
414 rdf:type schema:CreativeWork
415 https://doi.org/10.1128/jvi.03246-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062713180
416 rdf:type schema:CreativeWork
417 https://doi.org/10.1128/jvi.03608-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032392977
418 rdf:type schema:CreativeWork
419 https://doi.org/10.1128/jvi.06745-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025531035
420 rdf:type schema:CreativeWork
421 https://doi.org/10.1128/jvi.79.16.10108-10125.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014523492
422 rdf:type schema:CreativeWork
423 https://doi.org/10.1155/2012/980250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027019318
424 rdf:type schema:CreativeWork
425 https://doi.org/10.1371/journal.ppat.1003618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046399042
426 rdf:type schema:CreativeWork
427 https://doi.org/10.1371/journal.ppat.1005520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009150169
428 rdf:type schema:CreativeWork
429 https://doi.org/10.1371/journal.ppat.1006212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083856519
430 rdf:type schema:CreativeWork
431 https://doi.org/10.1529/biophysj.106.091561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049396823
432 rdf:type schema:CreativeWork
433 https://doi.org/10.3389/fimmu.2014.00520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041053562
434 rdf:type schema:CreativeWork
435 https://www.grid.ac/institutes/grid.164295.d schema:alternateName University of Maryland, College Park
436 schema:name Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA
437 Virology Program at the University of Maryland, 20740, College Park, MD, USA
438 rdf:type schema:Organization
439 https://www.grid.ac/institutes/grid.214007.0 schema:alternateName Scripps Research Institute
440 schema:name Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 92037, La Jolla, CA, USA
441 IAVI Neutralizing Antibody Center, The Scripps Research Institute, 92037, La Jolla, CA, USA
442 rdf:type schema:Organization
443 https://www.grid.ac/institutes/grid.411024.2 schema:alternateName University of Maryland, Baltimore
444 schema:name Department of Microbiology and Immunology, University of Maryland School of Medicine, 21201, Baltimore, MD, USA
445 Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA
446 Virology Program at the University of Maryland, 20740, College Park, MD, USA
447 rdf:type schema:Organization
448 https://www.grid.ac/institutes/grid.419681.3 schema:alternateName National Institute of Allergy and Infectious Diseases
449 schema:name Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA
450 rdf:type schema:Organization
451 https://www.grid.ac/institutes/grid.440664.4 schema:alternateName Universities at Shady Grove
452 schema:name Institute for Bioscience and Biotechnology Research, University of Maryland, 20850, Rockville, MD, USA
453 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...