Cryo-EM structure of a licensed DNA replication origin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa

ABSTRACT

Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or phosphorylated, and visualize a phospho-dependent MCM element likely important for Cdc45 recruitment. MCM pore loops touch both the Watson and Crick strands, constraining duplex DNA in a bent configuration. By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication. More... »

PAGES

2241

References to SciGraph publications

  • 2017-03. Open-ringed structure of the Cdt1–Mcm2–7 complex as a precursor of the MCM double hexamer in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2016-03. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2015-08. Structure of the eukaryotic MCM complex at 3.8 Å in NATURE
  • 2017-03. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1 in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2005-09. Organization of the archaeal MCM complex on DNA and implications for the helicase mechanism in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2007-01. Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast in NATURE
  • 2010-01-07. The Dbf4–Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 in NATURE
  • 2014-01. Quantifying the local resolution of cryo-EM density maps in NATURE METHODS
  • 2017-04. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy in NATURE METHODS
  • 2011-10. DNA stretching by bacterial initiators promotes replication origin opening in NATURE
  • 2013-03. ATPase-dependent quality control of DNA replication origin licensing in NATURE
  • 2015-03. Regulated eukaryotic DNA replication origin firing with purified proteins in NATURE
  • 2017-06-23. Cdt1 stabilizes an open MCM ring for helicase loading in NATURE COMMUNICATIONS
  • 2016-02-18. Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate in NATURE COMMUNICATIONS
  • 2017-03. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination in NATURE METHODS
  • 2015-03. Crystal structure of the eukaryotic origin recognition complex in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-017-02389-0

    DOI

    http://dx.doi.org/10.1038/s41467-017-02389-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1099740171

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29269875


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Cycle Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cryoelectron Microscopy", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Helicases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Replication", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA-Binding Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Holoenzymes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Image Processing, Computer-Assisted", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Minichromosome Maintenance Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nuclear Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phosphorylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Structure, Quaternary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein-Serine-Threonine Kinases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saccharomyces cerevisiae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saccharomyces cerevisiae Proteins", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Macromolecular Machines Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ali", 
            "givenName": "Ferdos Abid", 
            "id": "sg:person.013012430337.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013012430337.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Douglas", 
            "givenName": "Max E.", 
            "id": "sg:person.0636510075.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636510075.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Macromolecular Machines Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Locke", 
            "givenName": "Julia", 
            "id": "sg:person.010263547755.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010263547755.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pye", 
            "givenName": "Valerie E.", 
            "id": "sg:person.0617734234.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617734234.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Structural Biology of Cells and Viruses, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nans", 
            "givenName": "Andrea", 
            "id": "sg:person.01342325675.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342325675.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Diffley", 
            "givenName": "John F. X.", 
            "id": "sg:person.01070163466.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070163466.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Francis Crick Institute", 
              "id": "https://www.grid.ac/institutes/grid.451388.3", 
              "name": [
                "Macromolecular Machines Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Costa", 
            "givenName": "Alessandro", 
            "id": "sg:person.0766340761.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766340761.42"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1107/s0907444910007493", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444910007493", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11920", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003048912", 
              "https://doi.org/10.1038/nature11920"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biophys.35.040405.101933", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003637962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1422003112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004167779"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m706300200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006882347"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14285", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007234009", 
              "https://doi.org/10.1038/nature14285"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcc.20084", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008225564"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0602400103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009060164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.18722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009291458"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2727", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009301311", 
              "https://doi.org/10.1038/nmeth.2727"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.01993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013205061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013948145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013948145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013948145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013948145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.10.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014449249"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05432", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017905987", 
              "https://doi.org/10.1038/nature05432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05432", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017905987", 
              "https://doi.org/10.1038/nature05432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909052925", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020777046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909052925", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020777046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021183882", 
              "https://doi.org/10.1038/nature10455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2014.06.033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021878467"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022812644", 
              "https://doi.org/10.1038/nature08647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022812644", 
              "https://doi.org/10.1038/nature08647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.11182", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023050774"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms10708", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024121020", 
              "https://doi.org/10.1038/ncomms10708"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.15252/embj.201593552", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031154978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s1399004715010457", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031828554"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2014.06.034", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032478305"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2016.01.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032991148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb974", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034811191", 
              "https://doi.org/10.1038/nsmb974"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb974", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034811191", 
              "https://doi.org/10.1038/nsmb974"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.1933010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035610853"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.1883410", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036815090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14685", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039721868", 
              "https://doi.org/10.1038/nature14685"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/embj.201387369", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040075478"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sbi.2016.01.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040099715"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2006.05.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040267501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14239", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041462769", 
              "https://doi.org/10.1038/nature14239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.03273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044177281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0911500106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044523994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2015.08.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045219124"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1620500114", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045519185"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.18129", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046590609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.18129", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046590609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2015.11.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049504602"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sbi.2011.01.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050985160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.3170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053369012", 
              "https://doi.org/10.1038/nsmb.3170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/10409238.2016.1274717", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079393610"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.4169", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083686092", 
              "https://doi.org/10.1038/nmeth.4169"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.3374", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083801556", 
              "https://doi.org/10.1038/nsmb.3374"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.3372", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083803663", 
              "https://doi.org/10.1038/nsmb.3372"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.aah6317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083845112"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.aah6317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083845112"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.4193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084129286", 
              "https://doi.org/10.1038/nmeth.4193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acs.biochem.6b00922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085304988"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.dnarep.2017.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085455644"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.dnarep.2017.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085455644"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.dnarep.2017.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085455644"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.dnarep.2017.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085455644"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms15720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1086122047", 
              "https://doi.org/10.1038/ncomms15720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2017.06.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090835245"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-12", 
        "datePublishedReg": "2017-12-01", 
        "description": "Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or phosphorylated, and visualize a phospho-dependent MCM element likely important for Cdc45 recruitment. MCM pore loops touch both the Watson and Crick strands, constraining duplex DNA in a bent configuration. By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41467-017-02389-0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4580274", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.4273647", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6798027", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6798155", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1043282", 
            "issn": [
              "2041-1723"
            ], 
            "name": "Nature Communications", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "8"
          }
        ], 
        "name": "Cryo-EM structure of a licensed DNA replication origin", 
        "pagination": "2241", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "8c4012eaf97ed1a8107dc81607e034442ab8093916071018b594aecbd86c2799"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "29269875"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101528555"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41467-017-02389-0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1099740171"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41467-017-02389-0", 
          "https://app.dimensions.ai/details/publication/pub.1099740171"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000577.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41467-017-02389-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02389-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02389-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02389-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02389-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    355 TRIPLES      21 PREDICATES      96 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41467-017-02389-0 schema:about N100cbaa74512474da104ba56cf69bcbe
    2 N20b2825da96a49ec9ddb94eabed276ef
    3 N2c003a91fb35477790ea22c00ed55714
    4 N313707c251724aa8a5e455d3b4842657
    5 N3270b3130a28435098df9fcd01b35111
    6 N52c8c599becd4af68fb4c25c38951dd0
    7 N5a4ec814cb5d43c9a0b81c328a2f005d
    8 N5ffba67248f94ee8b0bf5885f8238b0e
    9 N6cdb67775c0f42c7974de6e756cab538
    10 N83f8c9c6d7ee44278f7c8b5fd57b640b
    11 N99326d8edcc548668ff49fb752cb612f
    12 Na1d5c8cedccc407caa7f1fe2f8a14b4c
    13 Nb206f2e34c4245728ba3000c50d2a263
    14 Nc19d51b0c01b432796978001705562c6
    15 Nc3f47c3a2c11486e819a4cf99542bac2
    16 Ncc7316f045bd43bd8b1a99a97721f3b7
    17 Nd3c831bb5d6a4e97af451d18e942f441
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author Ncc59e5a198894eb1b7148ac8a742bbd0
    21 schema:citation sg:pub.10.1038/nature05432
    22 sg:pub.10.1038/nature08647
    23 sg:pub.10.1038/nature10455
    24 sg:pub.10.1038/nature11920
    25 sg:pub.10.1038/nature14239
    26 sg:pub.10.1038/nature14285
    27 sg:pub.10.1038/nature14685
    28 sg:pub.10.1038/ncomms10708
    29 sg:pub.10.1038/ncomms15720
    30 sg:pub.10.1038/nmeth.2727
    31 sg:pub.10.1038/nmeth.4169
    32 sg:pub.10.1038/nmeth.4193
    33 sg:pub.10.1038/nsmb.3170
    34 sg:pub.10.1038/nsmb.3372
    35 sg:pub.10.1038/nsmb.3374
    36 sg:pub.10.1038/nsmb974
    37 https://doi.org/10.1002/embj.201387369
    38 https://doi.org/10.1002/jcc.20084
    39 https://doi.org/10.1016/j.cell.2009.10.015
    40 https://doi.org/10.1016/j.dnarep.2017.05.005
    41 https://doi.org/10.1016/j.jmb.2016.01.024
    42 https://doi.org/10.1016/j.jsb.2006.05.009
    43 https://doi.org/10.1016/j.jsb.2015.08.008
    44 https://doi.org/10.1016/j.jsb.2015.11.003
    45 https://doi.org/10.1016/j.molcel.2009.12.030
    46 https://doi.org/10.1016/j.molcel.2014.06.033
    47 https://doi.org/10.1016/j.molcel.2014.06.034
    48 https://doi.org/10.1016/j.molcel.2017.06.016
    49 https://doi.org/10.1016/j.sbi.2011.01.002
    50 https://doi.org/10.1016/j.sbi.2016.01.014
    51 https://doi.org/10.1021/acs.biochem.6b00922
    52 https://doi.org/10.1073/pnas.0602400103
    53 https://doi.org/10.1073/pnas.0911500106
    54 https://doi.org/10.1073/pnas.1422003112
    55 https://doi.org/10.1073/pnas.1620500114
    56 https://doi.org/10.1074/jbc.m706300200
    57 https://doi.org/10.1080/10409238.2016.1274717
    58 https://doi.org/10.1101/gad.1883410
    59 https://doi.org/10.1101/gad.1933010
    60 https://doi.org/10.1107/s0907444909052925
    61 https://doi.org/10.1107/s0907444910007493
    62 https://doi.org/10.1107/s1399004715010457
    63 https://doi.org/10.1126/science.aah6317
    64 https://doi.org/10.1146/annurev.biophys.35.040405.101933
    65 https://doi.org/10.15252/embj.201593552
    66 https://doi.org/10.7554/elife.01993
    67 https://doi.org/10.7554/elife.03273
    68 https://doi.org/10.7554/elife.11182
    69 https://doi.org/10.7554/elife.18129
    70 https://doi.org/10.7554/elife.18722
    71 schema:datePublished 2017-12
    72 schema:datePublishedReg 2017-12-01
    73 schema:description Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or phosphorylated, and visualize a phospho-dependent MCM element likely important for Cdc45 recruitment. MCM pore loops touch both the Watson and Crick strands, constraining duplex DNA in a bent configuration. By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication.
    74 schema:genre research_article
    75 schema:inLanguage en
    76 schema:isAccessibleForFree true
    77 schema:isPartOf N5d8dbed3cd6d4bc48d2b606e407422e7
    78 N7f8202ddeb724745ac15a68fece1a833
    79 sg:journal.1043282
    80 schema:name Cryo-EM structure of a licensed DNA replication origin
    81 schema:pagination 2241
    82 schema:productId N2d7a735dad6b4878a8a998c0725a4f41
    83 N4b0838abd27849859325472f9bd15a5e
    84 N783c5a193ac34378994679d06a1b9806
    85 N862c768ff30044ce869b512c1124e14c
    86 Na56d50661dc34590a608ea653bd7a1af
    87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099740171
    88 https://doi.org/10.1038/s41467-017-02389-0
    89 schema:sdDatePublished 2019-04-10T16:52
    90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    91 schema:sdPublisher N22d7aeedf236496996cf3916d032ccd4
    92 schema:url https://www.nature.com/articles/s41467-017-02389-0
    93 sgo:license sg:explorer/license/
    94 sgo:sdDataset articles
    95 rdf:type schema:ScholarlyArticle
    96 N100cbaa74512474da104ba56cf69bcbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Saccharomyces cerevisiae Proteins
    98 rdf:type schema:DefinedTerm
    99 N1152865901c244f5a21f2b9302c93b96 rdf:first sg:person.010263547755.63
    100 rdf:rest N21d89299b9d946689f264f3a18b59562
    101 N20b2825da96a49ec9ddb94eabed276ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name DNA
    103 rdf:type schema:DefinedTerm
    104 N21d89299b9d946689f264f3a18b59562 rdf:first sg:person.0617734234.88
    105 rdf:rest Na098d8541edb4c99af6dcba25988f556
    106 N22d7aeedf236496996cf3916d032ccd4 schema:name Springer Nature - SN SciGraph project
    107 rdf:type schema:Organization
    108 N2c003a91fb35477790ea22c00ed55714 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Holoenzymes
    110 rdf:type schema:DefinedTerm
    111 N2d7a735dad6b4878a8a998c0725a4f41 schema:name nlm_unique_id
    112 schema:value 101528555
    113 rdf:type schema:PropertyValue
    114 N313707c251724aa8a5e455d3b4842657 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Nucleic Acid Conformation
    116 rdf:type schema:DefinedTerm
    117 N3270b3130a28435098df9fcd01b35111 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name DNA-Binding Proteins
    119 rdf:type schema:DefinedTerm
    120 N4b0838abd27849859325472f9bd15a5e schema:name readcube_id
    121 schema:value 8c4012eaf97ed1a8107dc81607e034442ab8093916071018b594aecbd86c2799
    122 rdf:type schema:PropertyValue
    123 N52c8c599becd4af68fb4c25c38951dd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Cell Cycle Proteins
    125 rdf:type schema:DefinedTerm
    126 N5a4ec814cb5d43c9a0b81c328a2f005d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Protein Conformation
    128 rdf:type schema:DefinedTerm
    129 N5d8dbed3cd6d4bc48d2b606e407422e7 schema:issueNumber 1
    130 rdf:type schema:PublicationIssue
    131 N5ffba67248f94ee8b0bf5885f8238b0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name DNA Helicases
    133 rdf:type schema:DefinedTerm
    134 N6cdb67775c0f42c7974de6e756cab538 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Nuclear Proteins
    136 rdf:type schema:DefinedTerm
    137 N783c5a193ac34378994679d06a1b9806 schema:name doi
    138 schema:value 10.1038/s41467-017-02389-0
    139 rdf:type schema:PropertyValue
    140 N7f8202ddeb724745ac15a68fece1a833 schema:volumeNumber 8
    141 rdf:type schema:PublicationVolume
    142 N83f8c9c6d7ee44278f7c8b5fd57b640b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Protein-Serine-Threonine Kinases
    144 rdf:type schema:DefinedTerm
    145 N862c768ff30044ce869b512c1124e14c schema:name dimensions_id
    146 schema:value pub.1099740171
    147 rdf:type schema:PropertyValue
    148 N90069d358038402b8edf82fd3b9fe841 rdf:first sg:person.01070163466.42
    149 rdf:rest Nac22939dbc6a4f62a4d0c8b58b0936a3
    150 N99326d8edcc548668ff49fb752cb612f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Protein Structure, Quaternary
    152 rdf:type schema:DefinedTerm
    153 Na098d8541edb4c99af6dcba25988f556 rdf:first sg:person.01342325675.49
    154 rdf:rest N90069d358038402b8edf82fd3b9fe841
    155 Na1d5c8cedccc407caa7f1fe2f8a14b4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Cryoelectron Microscopy
    157 rdf:type schema:DefinedTerm
    158 Na56d50661dc34590a608ea653bd7a1af schema:name pubmed_id
    159 schema:value 29269875
    160 rdf:type schema:PropertyValue
    161 Nac22939dbc6a4f62a4d0c8b58b0936a3 rdf:first sg:person.0766340761.42
    162 rdf:rest rdf:nil
    163 Nb073e70ede12488dadc3ea6eb25383da rdf:first sg:person.0636510075.76
    164 rdf:rest N1152865901c244f5a21f2b9302c93b96
    165 Nb206f2e34c4245728ba3000c50d2a263 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Minichromosome Maintenance Proteins
    167 rdf:type schema:DefinedTerm
    168 Nc19d51b0c01b432796978001705562c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Image Processing, Computer-Assisted
    170 rdf:type schema:DefinedTerm
    171 Nc3f47c3a2c11486e819a4cf99542bac2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Saccharomyces cerevisiae
    173 rdf:type schema:DefinedTerm
    174 Ncc59e5a198894eb1b7148ac8a742bbd0 rdf:first sg:person.013012430337.05
    175 rdf:rest Nb073e70ede12488dadc3ea6eb25383da
    176 Ncc7316f045bd43bd8b1a99a97721f3b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Phosphorylation
    178 rdf:type schema:DefinedTerm
    179 Nd3c831bb5d6a4e97af451d18e942f441 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name DNA Replication
    181 rdf:type schema:DefinedTerm
    182 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    183 schema:name Biological Sciences
    184 rdf:type schema:DefinedTerm
    185 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    186 schema:name Biochemistry and Cell Biology
    187 rdf:type schema:DefinedTerm
    188 sg:grant.4273647 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02389-0
    189 rdf:type schema:MonetaryGrant
    190 sg:grant.4580274 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02389-0
    191 rdf:type schema:MonetaryGrant
    192 sg:grant.6798027 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02389-0
    193 rdf:type schema:MonetaryGrant
    194 sg:grant.6798155 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02389-0
    195 rdf:type schema:MonetaryGrant
    196 sg:journal.1043282 schema:issn 2041-1723
    197 schema:name Nature Communications
    198 rdf:type schema:Periodical
    199 sg:person.010263547755.63 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    200 schema:familyName Locke
    201 schema:givenName Julia
    202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010263547755.63
    203 rdf:type schema:Person
    204 sg:person.01070163466.42 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    205 schema:familyName Diffley
    206 schema:givenName John F. X.
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070163466.42
    208 rdf:type schema:Person
    209 sg:person.013012430337.05 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    210 schema:familyName Ali
    211 schema:givenName Ferdos Abid
    212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013012430337.05
    213 rdf:type schema:Person
    214 sg:person.01342325675.49 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    215 schema:familyName Nans
    216 schema:givenName Andrea
    217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342325675.49
    218 rdf:type schema:Person
    219 sg:person.0617734234.88 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    220 schema:familyName Pye
    221 schema:givenName Valerie E.
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617734234.88
    223 rdf:type schema:Person
    224 sg:person.0636510075.76 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    225 schema:familyName Douglas
    226 schema:givenName Max E.
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636510075.76
    228 rdf:type schema:Person
    229 sg:person.0766340761.42 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
    230 schema:familyName Costa
    231 schema:givenName Alessandro
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766340761.42
    233 rdf:type schema:Person
    234 sg:pub.10.1038/nature05432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017905987
    235 https://doi.org/10.1038/nature05432
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1038/nature08647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022812644
    238 https://doi.org/10.1038/nature08647
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1038/nature10455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021183882
    241 https://doi.org/10.1038/nature10455
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/nature11920 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003048912
    244 https://doi.org/10.1038/nature11920
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/nature14239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041462769
    247 https://doi.org/10.1038/nature14239
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/nature14285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007234009
    250 https://doi.org/10.1038/nature14285
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nature14685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039721868
    253 https://doi.org/10.1038/nature14685
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/ncomms10708 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024121020
    256 https://doi.org/10.1038/ncomms10708
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/ncomms15720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086122047
    259 https://doi.org/10.1038/ncomms15720
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nmeth.2727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009301311
    262 https://doi.org/10.1038/nmeth.2727
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nmeth.4169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083686092
    265 https://doi.org/10.1038/nmeth.4169
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nmeth.4193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084129286
    268 https://doi.org/10.1038/nmeth.4193
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/nsmb.3170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053369012
    271 https://doi.org/10.1038/nsmb.3170
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/nsmb.3372 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083803663
    274 https://doi.org/10.1038/nsmb.3372
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/nsmb.3374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083801556
    277 https://doi.org/10.1038/nsmb.3374
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/nsmb974 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034811191
    280 https://doi.org/10.1038/nsmb974
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1002/embj.201387369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040075478
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1002/jcc.20084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008225564
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1016/j.cell.2009.10.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014449249
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1016/j.dnarep.2017.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085455644
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1016/j.jmb.2016.01.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032991148
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1016/j.jsb.2006.05.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040267501
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1016/j.jsb.2015.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045219124
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1016/j.jsb.2015.11.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049504602
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1016/j.molcel.2009.12.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013948145
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1016/j.molcel.2014.06.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021878467
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1016/j.molcel.2014.06.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032478305
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1016/j.molcel.2017.06.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090835245
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1016/j.sbi.2011.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050985160
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1016/j.sbi.2016.01.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040099715
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1021/acs.biochem.6b00922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085304988
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1073/pnas.0602400103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009060164
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1073/pnas.0911500106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044523994
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1073/pnas.1422003112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004167779
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1073/pnas.1620500114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045519185
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1074/jbc.m706300200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006882347
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.1080/10409238.2016.1274717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079393610
    323 rdf:type schema:CreativeWork
    324 https://doi.org/10.1101/gad.1883410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036815090
    325 rdf:type schema:CreativeWork
    326 https://doi.org/10.1101/gad.1933010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035610853
    327 rdf:type schema:CreativeWork
    328 https://doi.org/10.1107/s0907444909052925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020777046
    329 rdf:type schema:CreativeWork
    330 https://doi.org/10.1107/s0907444910007493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235100
    331 rdf:type schema:CreativeWork
    332 https://doi.org/10.1107/s1399004715010457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031828554
    333 rdf:type schema:CreativeWork
    334 https://doi.org/10.1126/science.aah6317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083845112
    335 rdf:type schema:CreativeWork
    336 https://doi.org/10.1146/annurev.biophys.35.040405.101933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003637962
    337 rdf:type schema:CreativeWork
    338 https://doi.org/10.15252/embj.201593552 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031154978
    339 rdf:type schema:CreativeWork
    340 https://doi.org/10.7554/elife.01993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013205061
    341 rdf:type schema:CreativeWork
    342 https://doi.org/10.7554/elife.03273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044177281
    343 rdf:type schema:CreativeWork
    344 https://doi.org/10.7554/elife.11182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023050774
    345 rdf:type schema:CreativeWork
    346 https://doi.org/10.7554/elife.18129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046590609
    347 rdf:type schema:CreativeWork
    348 https://doi.org/10.7554/elife.18722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009291458
    349 rdf:type schema:CreativeWork
    350 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
    351 schema:name Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK
    352 Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK
    353 Macromolecular Machines Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK
    354 Structural Biology of Cells and Viruses, The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, UK
    355 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...