Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Sarah Batson, Cesira de Chiara, Vita Majce, Adrian J. Lloyd, Stanislav Gobec, Dean Rea, Vilmos Fülöp, Christopher W. Thoroughgood, Katie J. Simmons, Christopher G. Dowson, Colin W. G. Fishwick, Luiz Pedro S. de Carvalho, David I. Roper

ABSTRACT

D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure. More... »

PAGES

1939

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-017-02118-7

DOI

http://dx.doi.org/10.1038/s41467-017-02118-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1093099489

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29208891


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alanine Racemase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibiotics, Antitubercular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cycloserine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Synthases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Batson", 
        "givenName": "Sarah", 
        "id": "sg:person.01153646624.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153646624.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Chiara", 
        "givenName": "Cesira", 
        "id": "sg:person.0766553704.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766553704.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Ljubljana", 
          "id": "https://www.grid.ac/institutes/grid.8954.0", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK", 
            "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, A\u0161ker\u010deva 7, 1000, Ljubljana, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Majce", 
        "givenName": "Vita", 
        "id": "sg:person.01037420224.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037420224.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lloyd", 
        "givenName": "Adrian J.", 
        "id": "sg:person.0736701743.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736701743.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Ljubljana", 
          "id": "https://www.grid.ac/institutes/grid.8954.0", 
          "name": [
            "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, A\u0161ker\u010deva 7, 1000, Ljubljana, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gobec", 
        "givenName": "Stanislav", 
        "id": "sg:person.01253021264.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253021264.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rea", 
        "givenName": "Dean", 
        "id": "sg:person.01252704171.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252704171.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "F\u00fcl\u00f6p", 
        "givenName": "Vilmos", 
        "id": "sg:person.01123253220.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123253220.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thoroughgood", 
        "givenName": "Christopher W.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leeds", 
          "id": "https://www.grid.ac/institutes/grid.9909.9", 
          "name": [
            "School of Chemistry, University of Leeds, Leeds LS2 9JT, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Simmons", 
        "givenName": "Katie J.", 
        "id": "sg:person.0626565270.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626565270.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dowson", 
        "givenName": "Christopher G.", 
        "id": "sg:person.010427066077.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010427066077.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leeds", 
          "id": "https://www.grid.ac/institutes/grid.9909.9", 
          "name": [
            "School of Chemistry, University of Leeds, Leeds LS2 9JT, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fishwick", 
        "givenName": "Colin W. G.", 
        "id": "sg:person.015042707607.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015042707607.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Carvalho", 
        "givenName": "Luiz Pedro S.", 
        "id": "sg:person.01337215646.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337215646.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Warwick", 
          "id": "https://www.grid.ac/institutes/grid.7372.1", 
          "name": [
            "School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roper", 
        "givenName": "David I.", 
        "id": "sg:person.010601142417.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601142417.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0076-6879(95)49043-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001780789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10388", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004674718", 
          "https://doi.org/10.1038/nature10388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004804347", 
          "https://doi.org/10.1038/nrmicro2349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004804347", 
          "https://doi.org/10.1038/nrmicro2349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd2201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005793977", 
          "https://doi.org/10.1038/nrd2201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd2201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005793977", 
          "https://doi.org/10.1038/nrd2201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-5521(00)00135-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007074030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0108767390010224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014210169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444996012255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020405374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444998012645", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024394066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/17460441.2013.743991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027598039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.150116497", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029885621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m404603200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030965995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.75.1.200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034687461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdg303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036741280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1744309110003970", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037183150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1744309110003970", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037183150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/8263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037336361", 
          "https://doi.org/10.1038/8263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmgm.2006.06.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038705727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ja.2013.49", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042260225", 
          "https://doi.org/10.1038/ja.2013.49"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ja.2013.49", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042260225", 
          "https://doi.org/10.1038/ja.2013.49"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2007.06.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045586780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd4572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048224960", 
          "https://doi.org/10.1038/nrd4572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/355472a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048571716", 
          "https://doi.org/10.1038/355472a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-5521(00)00116-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050739449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ml400349n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051494693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00892a001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055192681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi962431t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi962431t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja01186a057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055780314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja01482a025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055800760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja048189s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055837608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja048189s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055837608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7939684", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062650703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078444193", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080437381", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083186722", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-017-02118-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2774697", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2761785", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3634979", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4456373", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2771603", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6798099", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3634844", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine", 
    "pagination": "1939", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7d9a5635fdc1eb9505ceadef4ad7f82dfb248ddca332602581d14b550c59f919"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29208891"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-017-02118-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1093099489"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-017-02118-7", 
      "https://app.dimensions.ai/details/publication/pub.1093099489"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78938_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-017-02118-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02118-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02118-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02118-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-02118-7'


 

This table displays all metadata directly associated to this object as RDF triples.

300 TRIPLES      21 PREDICATES      67 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-017-02118-7 schema:about N0a829a48ddef45949b4bf116d5b135c0
2 N1292a749d41d42b7860471a3891c40a0
3 N50a6f59f7669440195b1ddea8d23814a
4 N62bc4f92d58f4190890ee5f8734a0c17
5 N79e02d9135e440bd969d438a89f77afb
6 Naae88b4dc51145af81d7922a37c9f1ba
7 Nd8c418d64e0a4887b2b0b061db51ddc1
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author N7b8dd9e2edfe434c90e5c04b272238c5
11 schema:citation sg:pub.10.1038/355472a0
12 sg:pub.10.1038/8263
13 sg:pub.10.1038/ja.2013.49
14 sg:pub.10.1038/nature10388
15 sg:pub.10.1038/nrd2201
16 sg:pub.10.1038/nrd4572
17 sg:pub.10.1038/nrmicro2349
18 https://app.dimensions.ai/details/publication/pub.1078444193
19 https://app.dimensions.ai/details/publication/pub.1080437381
20 https://app.dimensions.ai/details/publication/pub.1083186722
21 https://doi.org/10.1016/0076-6879(95)49043-4
22 https://doi.org/10.1016/j.jmgm.2006.06.002
23 https://doi.org/10.1016/j.neuint.2007.06.003
24 https://doi.org/10.1016/s1074-5521(00)00116-2
25 https://doi.org/10.1016/s1074-5521(00)00135-6
26 https://doi.org/10.1021/bi00892a001
27 https://doi.org/10.1021/bi962431t
28 https://doi.org/10.1021/ja01186a057
29 https://doi.org/10.1021/ja01482a025
30 https://doi.org/10.1021/ja048189s
31 https://doi.org/10.1021/ml400349n
32 https://doi.org/10.1073/pnas.150116497
33 https://doi.org/10.1073/pnas.75.1.200
34 https://doi.org/10.1074/jbc.m404603200
35 https://doi.org/10.1093/emboj/cdg303
36 https://doi.org/10.1107/s0108767390010224
37 https://doi.org/10.1107/s0907444996012255
38 https://doi.org/10.1107/s0907444998012645
39 https://doi.org/10.1107/s1744309110003970
40 https://doi.org/10.1126/science.7939684
41 https://doi.org/10.1517/17460441.2013.743991
42 schema:datePublished 2017-12
43 schema:datePublishedReg 2017-12-01
44 schema:description D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure.
45 schema:genre research_article
46 schema:inLanguage en
47 schema:isAccessibleForFree true
48 schema:isPartOf N146b1b2b62624961ae85e27d2972bbf4
49 Nd5bc02d01ead47b6bd43203111ce153c
50 sg:journal.1043282
51 schema:name Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine
52 schema:pagination 1939
53 schema:productId N39e6bee422574a8d800449ff2397ac4b
54 N9b04fef2efd34947a603369a2dca68ea
55 Nd5ecb34013a54483823d355b4ec51e14
56 Ne44ff9fe17aa45d3905d4cab4296dcbc
57 Nf49fba1171834ad2886b0173bb8bfc45
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093099489
59 https://doi.org/10.1038/s41467-017-02118-7
60 schema:sdDatePublished 2019-04-11T13:17
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher N52690c65a82040bcb751d702b076deea
63 schema:url https://www.nature.com/articles/s41467-017-02118-7
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N0714151e698d4f4c8551182e2fe3e2db rdf:first sg:person.0766553704.00
68 rdf:rest Ne5d1babe02ea4b0e948c8cee5623a7c2
69 N0a829a48ddef45949b4bf116d5b135c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Antibiotics, Antitubercular
71 rdf:type schema:DefinedTerm
72 N0bb3e92b31454f60b52ad9aab39fd095 rdf:first Ne8f1ff0be1ef433aa09f0ee139f26b3b
73 rdf:rest N567b8902852f4376a112483eb8904d7d
74 N1292a749d41d42b7860471a3891c40a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Phosphorylation
76 rdf:type schema:DefinedTerm
77 N146b1b2b62624961ae85e27d2972bbf4 schema:volumeNumber 8
78 rdf:type schema:PublicationVolume
79 N26fc720190ab4435af170e41e1f52cd8 rdf:first sg:person.015042707607.47
80 rdf:rest N860091199f004cf6bd20d84a22db163d
81 N39e6bee422574a8d800449ff2397ac4b schema:name dimensions_id
82 schema:value pub.1093099489
83 rdf:type schema:PropertyValue
84 N50a6f59f7669440195b1ddea8d23814a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Escherichia coli
86 rdf:type schema:DefinedTerm
87 N52690c65a82040bcb751d702b076deea schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N567b8902852f4376a112483eb8904d7d rdf:first sg:person.0626565270.24
90 rdf:rest Nda982432a8694aeba8a9d07a1eda7630
91 N62bc4f92d58f4190890ee5f8734a0c17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Escherichia coli Proteins
93 rdf:type schema:DefinedTerm
94 N69c79da472b7410693a1965431f7dea1 rdf:first sg:person.01253021264.93
95 rdf:rest Nc198210848bf46fab1ec0e9e5f18d14c
96 N79e02d9135e440bd969d438a89f77afb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Peptide Synthases
98 rdf:type schema:DefinedTerm
99 N7b8dd9e2edfe434c90e5c04b272238c5 rdf:first sg:person.01153646624.07
100 rdf:rest N0714151e698d4f4c8551182e2fe3e2db
101 N860091199f004cf6bd20d84a22db163d rdf:first sg:person.01337215646.23
102 rdf:rest Nf2e2cf45fbf1404c83294641c34e18ea
103 N9b04fef2efd34947a603369a2dca68ea schema:name readcube_id
104 schema:value 7d9a5635fdc1eb9505ceadef4ad7f82dfb248ddca332602581d14b550c59f919
105 rdf:type schema:PropertyValue
106 Naae88b4dc51145af81d7922a37c9f1ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Alanine Racemase
108 rdf:type schema:DefinedTerm
109 Nbf4f38f9fc124750a130af57234c3575 rdf:first sg:person.0736701743.21
110 rdf:rest N69c79da472b7410693a1965431f7dea1
111 Nc198210848bf46fab1ec0e9e5f18d14c rdf:first sg:person.01252704171.83
112 rdf:rest Nc967b2b5b48c438e95506740a777c152
113 Nc967b2b5b48c438e95506740a777c152 rdf:first sg:person.01123253220.52
114 rdf:rest N0bb3e92b31454f60b52ad9aab39fd095
115 Nd5bc02d01ead47b6bd43203111ce153c schema:issueNumber 1
116 rdf:type schema:PublicationIssue
117 Nd5ecb34013a54483823d355b4ec51e14 schema:name doi
118 schema:value 10.1038/s41467-017-02118-7
119 rdf:type schema:PropertyValue
120 Nd8c418d64e0a4887b2b0b061db51ddc1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Cycloserine
122 rdf:type schema:DefinedTerm
123 Nda982432a8694aeba8a9d07a1eda7630 rdf:first sg:person.010427066077.74
124 rdf:rest N26fc720190ab4435af170e41e1f52cd8
125 Ne44ff9fe17aa45d3905d4cab4296dcbc schema:name nlm_unique_id
126 schema:value 101528555
127 rdf:type schema:PropertyValue
128 Ne5d1babe02ea4b0e948c8cee5623a7c2 rdf:first sg:person.01037420224.79
129 rdf:rest Nbf4f38f9fc124750a130af57234c3575
130 Ne8f1ff0be1ef433aa09f0ee139f26b3b schema:affiliation https://www.grid.ac/institutes/grid.7372.1
131 schema:familyName Thoroughgood
132 schema:givenName Christopher W.
133 rdf:type schema:Person
134 Nf2e2cf45fbf1404c83294641c34e18ea rdf:first sg:person.010601142417.38
135 rdf:rest rdf:nil
136 Nf49fba1171834ad2886b0173bb8bfc45 schema:name pubmed_id
137 schema:value 29208891
138 rdf:type schema:PropertyValue
139 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
140 schema:name Biological Sciences
141 rdf:type schema:DefinedTerm
142 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biochemistry and Cell Biology
144 rdf:type schema:DefinedTerm
145 sg:grant.2761785 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
146 rdf:type schema:MonetaryGrant
147 sg:grant.2771603 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
148 rdf:type schema:MonetaryGrant
149 sg:grant.2774697 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
150 rdf:type schema:MonetaryGrant
151 sg:grant.3634844 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
152 rdf:type schema:MonetaryGrant
153 sg:grant.3634979 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
154 rdf:type schema:MonetaryGrant
155 sg:grant.4456373 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
156 rdf:type schema:MonetaryGrant
157 sg:grant.6798099 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-02118-7
158 rdf:type schema:MonetaryGrant
159 sg:journal.1043282 schema:issn 2041-1723
160 schema:name Nature Communications
161 rdf:type schema:Periodical
162 sg:person.01037420224.79 schema:affiliation https://www.grid.ac/institutes/grid.8954.0
163 schema:familyName Majce
164 schema:givenName Vita
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037420224.79
166 rdf:type schema:Person
167 sg:person.010427066077.74 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
168 schema:familyName Dowson
169 schema:givenName Christopher G.
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010427066077.74
171 rdf:type schema:Person
172 sg:person.010601142417.38 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
173 schema:familyName Roper
174 schema:givenName David I.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601142417.38
176 rdf:type schema:Person
177 sg:person.01123253220.52 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
178 schema:familyName Fülöp
179 schema:givenName Vilmos
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123253220.52
181 rdf:type schema:Person
182 sg:person.01153646624.07 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
183 schema:familyName Batson
184 schema:givenName Sarah
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153646624.07
186 rdf:type schema:Person
187 sg:person.01252704171.83 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
188 schema:familyName Rea
189 schema:givenName Dean
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252704171.83
191 rdf:type schema:Person
192 sg:person.01253021264.93 schema:affiliation https://www.grid.ac/institutes/grid.8954.0
193 schema:familyName Gobec
194 schema:givenName Stanislav
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253021264.93
196 rdf:type schema:Person
197 sg:person.01337215646.23 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
198 schema:familyName de Carvalho
199 schema:givenName Luiz Pedro S.
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337215646.23
201 rdf:type schema:Person
202 sg:person.015042707607.47 schema:affiliation https://www.grid.ac/institutes/grid.9909.9
203 schema:familyName Fishwick
204 schema:givenName Colin W. G.
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015042707607.47
206 rdf:type schema:Person
207 sg:person.0626565270.24 schema:affiliation https://www.grid.ac/institutes/grid.9909.9
208 schema:familyName Simmons
209 schema:givenName Katie J.
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626565270.24
211 rdf:type schema:Person
212 sg:person.0736701743.21 schema:affiliation https://www.grid.ac/institutes/grid.7372.1
213 schema:familyName Lloyd
214 schema:givenName Adrian J.
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736701743.21
216 rdf:type schema:Person
217 sg:person.0766553704.00 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
218 schema:familyName de Chiara
219 schema:givenName Cesira
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766553704.00
221 rdf:type schema:Person
222 sg:pub.10.1038/355472a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048571716
223 https://doi.org/10.1038/355472a0
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/8263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037336361
226 https://doi.org/10.1038/8263
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/ja.2013.49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042260225
229 https://doi.org/10.1038/ja.2013.49
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nature10388 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004674718
232 https://doi.org/10.1038/nature10388
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nrd2201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005793977
235 https://doi.org/10.1038/nrd2201
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/nrd4572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048224960
238 https://doi.org/10.1038/nrd4572
239 rdf:type schema:CreativeWork
240 sg:pub.10.1038/nrmicro2349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004804347
241 https://doi.org/10.1038/nrmicro2349
242 rdf:type schema:CreativeWork
243 https://app.dimensions.ai/details/publication/pub.1078444193 schema:CreativeWork
244 https://app.dimensions.ai/details/publication/pub.1080437381 schema:CreativeWork
245 https://app.dimensions.ai/details/publication/pub.1083186722 schema:CreativeWork
246 https://doi.org/10.1016/0076-6879(95)49043-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001780789
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.jmgm.2006.06.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038705727
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.neuint.2007.06.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045586780
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s1074-5521(00)00116-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050739449
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s1074-5521(00)00135-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007074030
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/bi00892a001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055192681
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/bi962431t schema:sameAs https://app.dimensions.ai/details/publication/pub.1055212985
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1021/ja01186a057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055780314
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1021/ja01482a025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055800760
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1021/ja048189s schema:sameAs https://app.dimensions.ai/details/publication/pub.1055837608
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1021/ml400349n schema:sameAs https://app.dimensions.ai/details/publication/pub.1051494693
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1073/pnas.150116497 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029885621
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1073/pnas.75.1.200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034687461
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1074/jbc.m404603200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030965995
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1093/emboj/cdg303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036741280
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1107/s0108767390010224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014210169
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1107/s0907444996012255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020405374
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1107/s0907444998012645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024394066
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1107/s1744309110003970 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037183150
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1126/science.7939684 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062650703
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1517/17460441.2013.743991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027598039
287 rdf:type schema:CreativeWork
288 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
289 schema:name Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute, NW1 1AT, London, UK
290 rdf:type schema:Organization
291 https://www.grid.ac/institutes/grid.7372.1 schema:alternateName University of Warwick
292 schema:name School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK
293 rdf:type schema:Organization
294 https://www.grid.ac/institutes/grid.8954.0 schema:alternateName University of Ljubljana
295 schema:name Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia
296 School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK
297 rdf:type schema:Organization
298 https://www.grid.ac/institutes/grid.9909.9 schema:alternateName University of Leeds
299 schema:name School of Chemistry, University of Leeds, Leeds LS2 9JT, UK
300 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...