Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates View Full Text


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Article Info

DATE

2017-11-17

AUTHORS

Arielle L. Yablonovitch, Jeremy Fu, Kexin Li, Simpla Mahato, Lin Kang, Eugenia Rashkovetsky, Abraham B. Korol, Hua Tang, Pawel Michalak, Andrew C. Zelhof, Eviatar Nevo, Jin Billy Li

ABSTRACT

Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from 'Evolution Canyon' in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences. More... »

PAGES

1570

References to SciGraph publications

  • 2002-12-23. Finishing a whole-genome shotgun: Release 3 of the Drosophila melanogaster euchromatic genome sequence in GENOME BIOLOGY
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  • 2000-07. Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2 in NATURE
  • 1999-05-06. Regulation of alternative splicing by RNA editing in NATURE
  • 2006-03-22. Adaptive differentiation of thermotolerance in Drosophila along a microclimatic gradient in HEREDITY
  • 2012-11-21. Population and sex differences in Drosophila melanogaster brain gene expression in BMC GENOMICS
  • 2009-03-04. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome in GENOME BIOLOGY
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  • 2015-09-16. Genetic mapping uncovers cis-regulatory landscape of RNA editing in NATURE COMMUNICATIONS
  • 2011-04-10. A framework for variation discovery and genotyping using next-generation DNA sequencing data in NATURE GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-017-01658-2

    DOI

    http://dx.doi.org/10.1038/s41467-017-01658-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1092669192

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29146998


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