Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Yihan Zhang, Wei Qin, Xiaochan Lu, Jason Xu, Haigen Huang, Haipeng Bai, Song Li, Shuo Lin

ABSTRACT

Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity. More... »

PAGES

118

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-017-00175-6

DOI

http://dx.doi.org/10.1038/s41467-017-00175-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1090780642

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28740134


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CRISPR-Cas Systems", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Editing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Growth Differentiation Factor 6", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Point Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reproducibility of Results", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Box Domain Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zebrafish", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zebrafish Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China", 
            "Department of Molecular, Cell and Developmental Biology, University of California, 90095, Los Angeles, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Yihan", 
        "id": "sg:person.01301620476.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301620476.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Peking University", 
          "id": "https://www.grid.ac/institutes/grid.11135.37", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qin", 
        "givenName": "Wei", 
        "id": "sg:person.01165372076.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165372076.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Peking University", 
          "id": "https://www.grid.ac/institutes/grid.11135.37", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lu", 
        "givenName": "Xiaochan", 
        "id": "sg:person.07773726545.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07773726545.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Department of Molecular, Cell and Developmental Biology, University of California, 90095, Los Angeles, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Jason", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Department of Molecular, Cell and Developmental Biology, University of California, 90095, Los Angeles, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Haigen", 
        "id": "sg:person.01174070073.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174070073.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Peking University", 
          "id": "https://www.grid.ac/institutes/grid.11135.37", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bai", 
        "givenName": "Haipeng", 
        "id": "sg:person.01030172133.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030172133.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Peking University", 
          "id": "https://www.grid.ac/institutes/grid.11135.37", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Song", 
        "id": "sg:person.0672442776.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672442776.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China", 
            "Department of Molecular, Cell and Developmental Biology, University of California, 90095, Los Angeles, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lin", 
        "givenName": "Shuo", 
        "id": "sg:person.011413572257.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011413572257.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nature13127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002821806", 
          "https://doi.org/10.1038/nature13127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002821806", 
          "https://doi.org/10.1038/nature13127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/g3.116.031914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007308957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/g3.116.031914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007308957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature17946", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009172001", 
          "https://doi.org/10.1038/nature17946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1231143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019873131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021191816", 
          "https://doi.org/10.1038/nature11537"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.161638.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021526947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1079512", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024409172"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024988605", 
          "https://doi.org/10.1038/nbt.2501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2374", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038038994", 
          "https://doi.org/10.1038/nmeth.2374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ajhg.2015.05.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041849759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2008.08.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042199702"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1308335110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043136564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292250", 
          "https://doi.org/10.1038/nature14592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aaf8729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049065760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.115584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050836359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2013.06.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051698837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.115.185041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067739551"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.115.185041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067739551"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.115.185041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067739551"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.115.185041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067739551"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079116246", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083803971", 
          "https://doi.org/10.1038/nbt.3803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2017.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084113361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2017.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084113361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3811", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084128647", 
          "https://doi.org/10.1038/nbt.3811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3816", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084128652", 
          "https://doi.org/10.1038/nbt.3816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3833", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084128666", 
          "https://doi.org/10.1038/nbt.3833"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable \"base editing\" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-017-00175-6", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3804131", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system", 
    "pagination": "118", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7ecc17fdadc53a12c80e77d4f3ba36566393c188e3ebac92d9bdfb839ec1ca31"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28740134"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-017-00175-6"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1090780642"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-017-00175-6", 
      "https://app.dimensions.ai/details/publication/pub.1090780642"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000552.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-017-00175-6"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-00175-6'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-00175-6'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-00175-6'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-017-00175-6'


 

This table displays all metadata directly associated to this object as RDF triples.

264 TRIPLES      21 PREDICATES      68 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-017-00175-6 schema:about N1ce5361c0f2c4e7da091d03e0beb2653
2 N36f7ccada7f7474cad5572a2fdf7e20e
3 N3e0c27df024242d1a26a0ba6b318c7fe
4 N4e65cbcfefba4d918ecc51171df25d92
5 N548e99bc65284b018368c4923a5231d9
6 N5d7205e878c74f39b2740b7de9bbaeca
7 N5f8ed51bb1e149deb815980e557bb671
8 N8e82f94bcaba428db09a618a2947101b
9 N92ae0d56fc5443baa8125ca67577f8fd
10 N9da33d28c77843518b876d831eb8a1a6
11 Nc2c5f74501f348c68325236ea72c454e
12 Ncdbf58e277b24d76b25b763c437307a1
13 Ne2a38c5f34164d7985c076cf5438a760
14 Neee1a39d4d5b4f5f9154fd453d4be3f3
15 Nef492e326dc2447f902a577aeb205eea
16 Nef9dfe70b1e04faa8aaa8186fb40d520
17 anzsrc-for:06
18 anzsrc-for:0604
19 schema:author Nfb9b34aaf3eb4ace923d85257078d628
20 schema:citation sg:pub.10.1038/nature11537
21 sg:pub.10.1038/nature13127
22 sg:pub.10.1038/nature14592
23 sg:pub.10.1038/nature17946
24 sg:pub.10.1038/nbt.2501
25 sg:pub.10.1038/nbt.3803
26 sg:pub.10.1038/nbt.3811
27 sg:pub.10.1038/nbt.3816
28 sg:pub.10.1038/nbt.3833
29 sg:pub.10.1038/nmeth.2374
30 https://app.dimensions.ai/details/publication/pub.1079116246
31 https://doi.org/10.1016/j.ajhg.2015.05.017
32 https://doi.org/10.1016/j.celrep.2013.06.020
33 https://doi.org/10.1016/j.molcel.2008.08.019
34 https://doi.org/10.1016/j.ymeth.2017.03.005
35 https://doi.org/10.1073/pnas.1308335110
36 https://doi.org/10.1101/gr.161638.113
37 https://doi.org/10.1126/science.1079512
38 https://doi.org/10.1126/science.1231143
39 https://doi.org/10.1126/science.aaf8729
40 https://doi.org/10.1242/dev.115584
41 https://doi.org/10.1534/g3.116.031914
42 https://doi.org/10.1534/genetics.115.185041
43 schema:datePublished 2017-12
44 schema:datePublishedReg 2017-12-01
45 schema:description Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf N6f9f6c8b835645f392f96b1393012878
50 Nafb9014d5d954561b2cfe8c2d684a138
51 sg:journal.1043282
52 schema:name Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
53 schema:pagination 118
54 schema:productId N42eeeee682794384bbd1437f9ba798c0
55 N8a859ec5968f4e7390564e940205e017
56 Na66d8928964a4c8f94f46591a4b5c6ab
57 Nc73198f901864573bad5d1c2167b90db
58 Nda06b12c75e742fd971ec223ec4ab101
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090780642
60 https://doi.org/10.1038/s41467-017-00175-6
61 schema:sdDatePublished 2019-04-11T02:21
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher Nd8275c19d3b14a01a5556b4c2c66cdeb
64 schema:url https://www.nature.com/articles/s41467-017-00175-6
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N0ad50a6207144ed8870cb04534fb0881 rdf:first sg:person.011413572257.58
69 rdf:rest rdf:nil
70 N1c06ceb4fcb841c89d23f5bc2280c5de rdf:first sg:person.01174070073.26
71 rdf:rest N644242303f19407f86d03d92558211f7
72 N1ce5361c0f2c4e7da091d03e0beb2653 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Mutagenesis, Site-Directed
74 rdf:type schema:DefinedTerm
75 N36f7ccada7f7474cad5572a2fdf7e20e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Reproducibility of Results
77 rdf:type schema:DefinedTerm
78 N3e0c27df024242d1a26a0ba6b318c7fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Base Sequence
80 rdf:type schema:DefinedTerm
81 N42eeeee682794384bbd1437f9ba798c0 schema:name dimensions_id
82 schema:value pub.1090780642
83 rdf:type schema:PropertyValue
84 N4e65cbcfefba4d918ecc51171df25d92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Amino Acid Sequence
86 rdf:type schema:DefinedTerm
87 N548e99bc65284b018368c4923a5231d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Gene Editing
89 rdf:type schema:DefinedTerm
90 N5888cdfc90354c568493810b5da4b878 rdf:first sg:person.07773726545.34
91 rdf:rest N7dfaa699dbc04251b3d6b68806764bf2
92 N5d7205e878c74f39b2740b7de9bbaeca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name CRISPR-Cas Systems
94 rdf:type schema:DefinedTerm
95 N5f8ed51bb1e149deb815980e557bb671 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Point Mutation
97 rdf:type schema:DefinedTerm
98 N644242303f19407f86d03d92558211f7 rdf:first sg:person.01030172133.01
99 rdf:rest N6598cda63a534eb39d26c1bd6a1cb9c0
100 N6598cda63a534eb39d26c1bd6a1cb9c0 rdf:first sg:person.0672442776.26
101 rdf:rest N0ad50a6207144ed8870cb04534fb0881
102 N6f9f6c8b835645f392f96b1393012878 schema:issueNumber 1
103 rdf:type schema:PublicationIssue
104 N7122db5b71f74a1db4c6713d3de3342a schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
105 schema:familyName Xu
106 schema:givenName Jason
107 rdf:type schema:Person
108 N7dfaa699dbc04251b3d6b68806764bf2 rdf:first N7122db5b71f74a1db4c6713d3de3342a
109 rdf:rest N1c06ceb4fcb841c89d23f5bc2280c5de
110 N8a859ec5968f4e7390564e940205e017 schema:name nlm_unique_id
111 schema:value 101528555
112 rdf:type schema:PropertyValue
113 N8e82f94bcaba428db09a618a2947101b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Animals
115 rdf:type schema:DefinedTerm
116 N92ae0d56fc5443baa8125ca67577f8fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Sequence Homology, Nucleic Acid
118 rdf:type schema:DefinedTerm
119 N9da33d28c77843518b876d831eb8a1a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Zebrafish
121 rdf:type schema:DefinedTerm
122 Na66d8928964a4c8f94f46591a4b5c6ab schema:name pubmed_id
123 schema:value 28740134
124 rdf:type schema:PropertyValue
125 Nafb9014d5d954561b2cfe8c2d684a138 schema:volumeNumber 8
126 rdf:type schema:PublicationVolume
127 Nc2c5f74501f348c68325236ea72c454e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Fetal Proteins
129 rdf:type schema:DefinedTerm
130 Nc73198f901864573bad5d1c2167b90db schema:name doi
131 schema:value 10.1038/s41467-017-00175-6
132 rdf:type schema:PropertyValue
133 Ncdbf58e277b24d76b25b763c437307a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name T-Box Domain Proteins
135 rdf:type schema:DefinedTerm
136 Nd8275c19d3b14a01a5556b4c2c66cdeb schema:name Springer Nature - SN SciGraph project
137 rdf:type schema:Organization
138 Nda06b12c75e742fd971ec223ec4ab101 schema:name readcube_id
139 schema:value 7ecc17fdadc53a12c80e77d4f3ba36566393c188e3ebac92d9bdfb839ec1ca31
140 rdf:type schema:PropertyValue
141 Nde211ab36f54478ba8d74133196a6993 rdf:first sg:person.01165372076.35
142 rdf:rest N5888cdfc90354c568493810b5da4b878
143 Ne2a38c5f34164d7985c076cf5438a760 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Zebrafish Proteins
145 rdf:type schema:DefinedTerm
146 Neee1a39d4d5b4f5f9154fd453d4be3f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Genetic Engineering
148 rdf:type schema:DefinedTerm
149 Nef492e326dc2447f902a577aeb205eea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Sequence Homology, Amino Acid
151 rdf:type schema:DefinedTerm
152 Nef9dfe70b1e04faa8aaa8186fb40d520 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Growth Differentiation Factor 6
154 rdf:type schema:DefinedTerm
155 Nfb9b34aaf3eb4ace923d85257078d628 rdf:first sg:person.01301620476.71
156 rdf:rest Nde211ab36f54478ba8d74133196a6993
157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biological Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
161 schema:name Genetics
162 rdf:type schema:DefinedTerm
163 sg:grant.3804131 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-017-00175-6
164 rdf:type schema:MonetaryGrant
165 sg:journal.1043282 schema:issn 2041-1723
166 schema:name Nature Communications
167 rdf:type schema:Periodical
168 sg:person.01030172133.01 schema:affiliation https://www.grid.ac/institutes/grid.11135.37
169 schema:familyName Bai
170 schema:givenName Haipeng
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030172133.01
172 rdf:type schema:Person
173 sg:person.011413572257.58 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
174 schema:familyName Lin
175 schema:givenName Shuo
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011413572257.58
177 rdf:type schema:Person
178 sg:person.01165372076.35 schema:affiliation https://www.grid.ac/institutes/grid.11135.37
179 schema:familyName Qin
180 schema:givenName Wei
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165372076.35
182 rdf:type schema:Person
183 sg:person.01174070073.26 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
184 schema:familyName Huang
185 schema:givenName Haigen
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174070073.26
187 rdf:type schema:Person
188 sg:person.01301620476.71 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
189 schema:familyName Zhang
190 schema:givenName Yihan
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301620476.71
192 rdf:type schema:Person
193 sg:person.0672442776.26 schema:affiliation https://www.grid.ac/institutes/grid.11135.37
194 schema:familyName Li
195 schema:givenName Song
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672442776.26
197 rdf:type schema:Person
198 sg:person.07773726545.34 schema:affiliation https://www.grid.ac/institutes/grid.11135.37
199 schema:familyName Lu
200 schema:givenName Xiaochan
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07773726545.34
202 rdf:type schema:Person
203 sg:pub.10.1038/nature11537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021191816
204 https://doi.org/10.1038/nature11537
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/nature13127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002821806
207 https://doi.org/10.1038/nature13127
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/nature14592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047292250
210 https://doi.org/10.1038/nature14592
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/nature17946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009172001
213 https://doi.org/10.1038/nature17946
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/nbt.2501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024988605
216 https://doi.org/10.1038/nbt.2501
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nbt.3803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083803971
219 https://doi.org/10.1038/nbt.3803
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nbt.3811 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084128647
222 https://doi.org/10.1038/nbt.3811
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nbt.3816 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084128652
225 https://doi.org/10.1038/nbt.3816
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nbt.3833 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084128666
228 https://doi.org/10.1038/nbt.3833
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/nmeth.2374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038038994
231 https://doi.org/10.1038/nmeth.2374
232 rdf:type schema:CreativeWork
233 https://app.dimensions.ai/details/publication/pub.1079116246 schema:CreativeWork
234 https://doi.org/10.1016/j.ajhg.2015.05.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041849759
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.celrep.2013.06.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051698837
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.molcel.2008.08.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042199702
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.ymeth.2017.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084113361
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.1308335110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043136564
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1101/gr.161638.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021526947
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1126/science.1079512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024409172
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1126/science.1231143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019873131
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1126/science.aaf8729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049065760
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1242/dev.115584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050836359
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1534/g3.116.031914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007308957
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1534/genetics.115.185041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067739551
257 rdf:type schema:CreativeWork
258 https://www.grid.ac/institutes/grid.11135.37 schema:alternateName Peking University
259 schema:name Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China
260 rdf:type schema:Organization
261 https://www.grid.ac/institutes/grid.19006.3e schema:alternateName University of California Los Angeles
262 schema:name Department of Molecular, Cell and Developmental Biology, University of California, 90095, Los Angeles, CA, USA
263 Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 518055, Shenzhen, China
264 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...