Autophagy activation contributes to lipid accumulation in tubular epithelial cells during kidney fibrosis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Qi Yan, Yuan Song, Lu Zhang, Zhaowei Chen, Cheng Yang, Shan Liu, Xiaohan Yuan, Hongyu Gao, Guohua Ding, Huiming Wang

ABSTRACT

Sustained activation of autophagy and lipid accumulation in tubular epithelial cells (TECs) are both associated with the kidney fibrosis progression. Autophagy has been found involved in the lipid metabolism regulation through a bi-directional mechanism of inducing lipolysis as well as promoting lipid droplet formation. However, whether and how autophagy influences lipid accumulation in kidney fibrosis remain unclear. In the current study, we show that UUO-induced lipid accumulation in tubular cells was significantly reduced when the pharmacological inhibitor 3-MA or CQ was administrated both in vivo and in vitro. Of interest, colocalization of LDs and autophagosomes, as well as colocalization of LDs and lysosomes were undetected in UUO-induced fibrotic kidneys, although lysosome function remained robust, indicating the lipid accumulation is lipophagy-lysosome pathway independent. TGF-β1-induced lipid droplets formation in HK-2 cells were decreased when the Beclin-1 expression was silenced, implying that autophagy-upregulated lipid droplets formation is Beclin-1 dependent. Finally, CQ treatment of UUO-induced fibrotic kidneys reduced the expression of α-SMA and tubular cell apoptosis and rescued the expression of E-cadherin, which was associated with the ameliorated lipid deposition. Therefore, our work documented that autophagy promotes lipid droplet formation in TECs in a Beclin-1-dependent manner, which causes renal lipotoxicity and contributes to the progression of kidney fibrosis. More... »

PAGES

39

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41420-018-0065-2

DOI

http://dx.doi.org/10.1038/s41420-018-0065-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105180671

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30062051


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China", 
            "Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yan", 
        "givenName": "Qi", 
        "id": "sg:person.01136746146.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136746146.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Yuan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Lu", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Zhaowei", 
        "id": "sg:person.014074732367.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014074732367.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Cheng", 
        "id": "sg:person.012501771367.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012501771367.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei University for Nationalities", 
          "id": "https://www.grid.ac/institutes/grid.440771.1", 
          "name": [
            "Department of Nephrology, University Hospital of Hubei University for Nationalities, Enshi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Shan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yuan", 
        "givenName": "Xiaohan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gao", 
        "givenName": "Hongyu", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ding", 
        "givenName": "Guohua", 
        "id": "sg:person.01156022362.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156022362.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Renmin Hospital of Wuhan University", 
          "id": "https://www.grid.ac/institutes/grid.412632.0", 
          "name": [
            "Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Huiming", 
        "id": "sg:person.01261771452.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261771452.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/ki.2013.462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001054116", 
          "https://doi.org/10.1038/ki.2013.462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pmed.0020045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001880244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cmet.2011.03.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001967281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1440-1797.2011.01541.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003362659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.00022.2011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004554230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/auto.24893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006076250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s40620-016-0278-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007203408", 
          "https://doi.org/10.1007/s40620-016-0278-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.metabol.2014.02.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008577067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/embor.2011.260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009399363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010869485", 
          "https://doi.org/10.1038/nature07976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010869485", 
          "https://doi.org/10.1038/nature07976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2013.055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011137998", 
          "https://doi.org/10.1038/nprot.2013.055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2009.03.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011153229"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1681/asn.2013101068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011511233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1681/asn.2013101068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011511233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.308460", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012009266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.kint.2015.11.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012362979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci71863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014075787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cddis.2013.404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014361079", 
          "https://doi.org/10.1038/cddis.2013.404"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/auto.22930", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014738825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0630588100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017297859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2016.1166317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018482225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mnh.0b013e32833aa4ac", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021175577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mnh.0b013e32833aa4ac", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021175577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mnh.0b013e32833aa4ac", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021175577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2015.05.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024005824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.p040501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026482557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddm100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028741317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2015.01.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031444977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.kint.2016.04.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032627630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra1205406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033005246"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2015.1124226", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033073799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.ki.5001834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034040060", 
          "https://doi.org/10.1038/sj.ki.5001834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00467-015-3134-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034384183", 
          "https://doi.org/10.1007/s00467-015-3134-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(16)32064-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036312804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(16)32064-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036312804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2012.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036434831", 
          "https://doi.org/10.1038/cdd.2012.63"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2007.78", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037118993", 
          "https://doi.org/10.1038/cr.2007.78"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1409323111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041361316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2016.1190071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043022677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2015.04.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045465245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.3762", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045967711", 
          "https://doi.org/10.1038/nm.3762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/ajpath.2010.091012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046443611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep18944", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050914373", 
          "https://doi.org/10.1038/srep18944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/ajpath.2010.090345", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051500112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2337/db15-0473", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070729624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijmm.2013.1232", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071507470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijmm.2013.1232", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071507470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/auto.26563", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072289878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.1997.273.3.e650", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083154539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cmet.2017.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083751033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2017.1341464", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091247095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrneph.2017.129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092056746", 
          "https://doi.org/10.1038/nrneph.2017.129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1681/asn.2017030314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092132985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1681/asn.2017030314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092132985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1681/asn.2017030314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092132985"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "Sustained activation of autophagy and lipid accumulation in tubular epithelial cells (TECs) are both associated with the kidney fibrosis progression. Autophagy has been found involved in the lipid metabolism regulation through a bi-directional mechanism of inducing lipolysis as well as promoting lipid droplet formation. However, whether and how autophagy influences lipid accumulation in kidney fibrosis remain unclear. In the current study, we show that UUO-induced lipid accumulation in tubular cells was significantly reduced when the pharmacological inhibitor 3-MA or CQ was administrated both in vivo and in vitro. Of interest, colocalization of LDs and autophagosomes, as well as colocalization of LDs and lysosomes were undetected in UUO-induced fibrotic kidneys, although lysosome function remained robust, indicating the lipid accumulation is lipophagy-lysosome pathway independent. TGF-\u03b21-induced lipid droplets formation in HK-2 cells were decreased when the Beclin-1 expression was silenced, implying that autophagy-upregulated lipid droplets formation is Beclin-1 dependent. Finally, CQ treatment of UUO-induced fibrotic kidneys reduced the expression of \u03b1-SMA and tubular cell apoptosis and rescued the expression of E-cadherin, which was associated with the ameliorated lipid deposition. Therefore, our work documented that autophagy promotes lipid droplet formation in TECs in a Beclin-1-dependent manner, which causes renal lipotoxicity and contributes to the progression of kidney fibrosis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41420-018-0065-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7024305", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1052270", 
        "issn": [
          "2058-7716"
        ], 
        "name": "Cell Death Discovery", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "4"
      }
    ], 
    "name": "Autophagy activation contributes to lipid accumulation in tubular epithelial cells during kidney fibrosis", 
    "pagination": "39", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "43d6d7c1ea5d0a57dc7685d72d6562a2c3ea2797489a2d0890f55cf5a4eb928c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30062051"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101665035"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41420-018-0065-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1105180671"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41420-018-0065-2", 
      "https://app.dimensions.ai/details/publication/pub.1105180671"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000566.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41420-018-0065-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41420-018-0065-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41420-018-0065-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41420-018-0065-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41420-018-0065-2'


 

This table displays all metadata directly associated to this object as RDF triples.

290 TRIPLES      21 PREDICATES      77 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41420-018-0065-2 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Na4568f545a904e629f688d322897f086
4 schema:citation sg:pub.10.1007/s00467-015-3134-2
5 sg:pub.10.1007/s40620-016-0278-5
6 sg:pub.10.1038/cdd.2012.63
7 sg:pub.10.1038/cddis.2013.404
8 sg:pub.10.1038/cr.2007.78
9 sg:pub.10.1038/ki.2013.462
10 sg:pub.10.1038/nature07976
11 sg:pub.10.1038/nm.3762
12 sg:pub.10.1038/nprot.2013.055
13 sg:pub.10.1038/nrneph.2017.129
14 sg:pub.10.1038/sj.ki.5001834
15 sg:pub.10.1038/srep18944
16 https://doi.org/10.1016/j.bbrc.2009.03.039
17 https://doi.org/10.1016/j.cmet.2011.03.023
18 https://doi.org/10.1016/j.cmet.2017.01.004
19 https://doi.org/10.1016/j.cub.2015.04.004
20 https://doi.org/10.1016/j.devcel.2015.01.029
21 https://doi.org/10.1016/j.kint.2015.11.021
22 https://doi.org/10.1016/j.kint.2016.04.014
23 https://doi.org/10.1016/j.metabol.2014.02.010
24 https://doi.org/10.1016/j.tcb.2015.05.004
25 https://doi.org/10.1016/s0140-6736(16)32064-5
26 https://doi.org/10.1038/embor.2011.260
27 https://doi.org/10.1056/nejmra1205406
28 https://doi.org/10.1073/pnas.0630588100
29 https://doi.org/10.1073/pnas.1409323111
30 https://doi.org/10.1074/jbc.m111.308460
31 https://doi.org/10.1080/15548627.2015.1124226
32 https://doi.org/10.1080/15548627.2016.1166317
33 https://doi.org/10.1080/15548627.2016.1190071
34 https://doi.org/10.1080/15548627.2017.1341464
35 https://doi.org/10.1093/hmg/ddm100
36 https://doi.org/10.1097/mnh.0b013e32833aa4ac
37 https://doi.org/10.1111/j.1440-1797.2011.01541.x
38 https://doi.org/10.1152/ajpendo.1997.273.3.e650
39 https://doi.org/10.1152/ajprenal.00022.2011
40 https://doi.org/10.1172/jci71863
41 https://doi.org/10.1194/jlr.p040501
42 https://doi.org/10.1371/journal.pmed.0020045
43 https://doi.org/10.1681/asn.2013101068
44 https://doi.org/10.1681/asn.2017030314
45 https://doi.org/10.2337/db15-0473
46 https://doi.org/10.2353/ajpath.2010.090345
47 https://doi.org/10.2353/ajpath.2010.091012
48 https://doi.org/10.3892/ijmm.2013.1232
49 https://doi.org/10.4161/auto.22930
50 https://doi.org/10.4161/auto.24893
51 https://doi.org/10.4161/auto.26563
52 schema:datePublished 2018-12
53 schema:datePublishedReg 2018-12-01
54 schema:description Sustained activation of autophagy and lipid accumulation in tubular epithelial cells (TECs) are both associated with the kidney fibrosis progression. Autophagy has been found involved in the lipid metabolism regulation through a bi-directional mechanism of inducing lipolysis as well as promoting lipid droplet formation. However, whether and how autophagy influences lipid accumulation in kidney fibrosis remain unclear. In the current study, we show that UUO-induced lipid accumulation in tubular cells was significantly reduced when the pharmacological inhibitor 3-MA or CQ was administrated both in vivo and in vitro. Of interest, colocalization of LDs and autophagosomes, as well as colocalization of LDs and lysosomes were undetected in UUO-induced fibrotic kidneys, although lysosome function remained robust, indicating the lipid accumulation is lipophagy-lysosome pathway independent. TGF-β1-induced lipid droplets formation in HK-2 cells were decreased when the Beclin-1 expression was silenced, implying that autophagy-upregulated lipid droplets formation is Beclin-1 dependent. Finally, CQ treatment of UUO-induced fibrotic kidneys reduced the expression of α-SMA and tubular cell apoptosis and rescued the expression of E-cadherin, which was associated with the ameliorated lipid deposition. Therefore, our work documented that autophagy promotes lipid droplet formation in TECs in a Beclin-1-dependent manner, which causes renal lipotoxicity and contributes to the progression of kidney fibrosis.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree true
58 schema:isPartOf Nb7a1133dda6e4a8787f3490d2b9bc6cd
59 Nbe7b10599d7940f49521dafaa612e4ea
60 sg:journal.1052270
61 schema:name Autophagy activation contributes to lipid accumulation in tubular epithelial cells during kidney fibrosis
62 schema:pagination 39
63 schema:productId N1afd8ebb5e5d4aefbf523d07a2a034aa
64 N32c50bb5e65f461394ef35fd7253650b
65 N6aaf679865254fc897a618dff0af6651
66 N9378d9c396ab4b319113ee68a80051c9
67 N9d335b9eca5d45bbb77e19ac3055cc57
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105180671
69 https://doi.org/10.1038/s41420-018-0065-2
70 schema:sdDatePublished 2019-04-10T16:00
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher Nfa73dda6c4fb4284900e3fb7753a97bf
73 schema:url https://www.nature.com/articles/s41420-018-0065-2
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N141ebad5afbf4302b52c2e867fd18311 rdf:first Nf58f94d48f4c403bad6637cf4d1691eb
78 rdf:rest N2ec7352524ef487bb72f8691a866ca43
79 N1afd8ebb5e5d4aefbf523d07a2a034aa schema:name readcube_id
80 schema:value 43d6d7c1ea5d0a57dc7685d72d6562a2c3ea2797489a2d0890f55cf5a4eb928c
81 rdf:type schema:PropertyValue
82 N239bd6834c3748469f9eeba872612fae schema:affiliation https://www.grid.ac/institutes/grid.440771.1
83 schema:familyName Liu
84 schema:givenName Shan
85 rdf:type schema:Person
86 N2ec7352524ef487bb72f8691a866ca43 rdf:first Nbe18ea6efc1e43be8c5eb041a5bde3e5
87 rdf:rest Ndaaba225a5d7414c8b9064759c635c10
88 N32c50bb5e65f461394ef35fd7253650b schema:name nlm_unique_id
89 schema:value 101665035
90 rdf:type schema:PropertyValue
91 N4019b6a512c14e3bbbca778020088c2c schema:name Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
92 rdf:type schema:Organization
93 N44e8ca69b6d141ca8f06c77c1343bd0b rdf:first sg:person.01261771452.38
94 rdf:rest rdf:nil
95 N670562da0acb45b890e60b41bde7a9ca rdf:first Nbb433d0973de48439cbf2d1eb09a9877
96 rdf:rest N745c55831ca3474e8757618d720e3bba
97 N6aaf679865254fc897a618dff0af6651 schema:name pubmed_id
98 schema:value 30062051
99 rdf:type schema:PropertyValue
100 N745c55831ca3474e8757618d720e3bba rdf:first Nc20a8e5e60414277badbe38eb6cb7be0
101 rdf:rest Nd386c2d08fb6434c9bb3faa98b6b70b4
102 N9378d9c396ab4b319113ee68a80051c9 schema:name doi
103 schema:value 10.1038/s41420-018-0065-2
104 rdf:type schema:PropertyValue
105 N9d335b9eca5d45bbb77e19ac3055cc57 schema:name dimensions_id
106 schema:value pub.1105180671
107 rdf:type schema:PropertyValue
108 Na4568f545a904e629f688d322897f086 rdf:first sg:person.01136746146.35
109 rdf:rest N141ebad5afbf4302b52c2e867fd18311
110 Na79ffe190c4a45ebacb0b5b88b87f00d rdf:first N239bd6834c3748469f9eeba872612fae
111 rdf:rest N670562da0acb45b890e60b41bde7a9ca
112 Nb7a1133dda6e4a8787f3490d2b9bc6cd schema:issueNumber 1
113 rdf:type schema:PublicationIssue
114 Nbb433d0973de48439cbf2d1eb09a9877 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
115 schema:familyName Yuan
116 schema:givenName Xiaohan
117 rdf:type schema:Person
118 Nbe18ea6efc1e43be8c5eb041a5bde3e5 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
119 schema:familyName Zhang
120 schema:givenName Lu
121 rdf:type schema:Person
122 Nbe7b10599d7940f49521dafaa612e4ea schema:volumeNumber 4
123 rdf:type schema:PublicationVolume
124 Nbff8a9ccb55945cc80b690427ea03ff7 rdf:first sg:person.012501771367.50
125 rdf:rest Na79ffe190c4a45ebacb0b5b88b87f00d
126 Nc20a8e5e60414277badbe38eb6cb7be0 schema:affiliation N4019b6a512c14e3bbbca778020088c2c
127 schema:familyName Gao
128 schema:givenName Hongyu
129 rdf:type schema:Person
130 Nd386c2d08fb6434c9bb3faa98b6b70b4 rdf:first sg:person.01156022362.66
131 rdf:rest N44e8ca69b6d141ca8f06c77c1343bd0b
132 Ndaaba225a5d7414c8b9064759c635c10 rdf:first sg:person.014074732367.82
133 rdf:rest Nbff8a9ccb55945cc80b690427ea03ff7
134 Nf58f94d48f4c403bad6637cf4d1691eb schema:affiliation https://www.grid.ac/institutes/grid.412632.0
135 schema:familyName Song
136 schema:givenName Yuan
137 rdf:type schema:Person
138 Nfa73dda6c4fb4284900e3fb7753a97bf schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
141 schema:name Biological Sciences
142 rdf:type schema:DefinedTerm
143 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biochemistry and Cell Biology
145 rdf:type schema:DefinedTerm
146 sg:grant.7024305 http://pending.schema.org/fundedItem sg:pub.10.1038/s41420-018-0065-2
147 rdf:type schema:MonetaryGrant
148 sg:journal.1052270 schema:issn 2058-7716
149 schema:name Cell Death Discovery
150 rdf:type schema:Periodical
151 sg:person.01136746146.35 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
152 schema:familyName Yan
153 schema:givenName Qi
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136746146.35
155 rdf:type schema:Person
156 sg:person.01156022362.66 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
157 schema:familyName Ding
158 schema:givenName Guohua
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156022362.66
160 rdf:type schema:Person
161 sg:person.012501771367.50 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
162 schema:familyName Yang
163 schema:givenName Cheng
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012501771367.50
165 rdf:type schema:Person
166 sg:person.01261771452.38 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
167 schema:familyName Wang
168 schema:givenName Huiming
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261771452.38
170 rdf:type schema:Person
171 sg:person.014074732367.82 schema:affiliation https://www.grid.ac/institutes/grid.412632.0
172 schema:familyName Chen
173 schema:givenName Zhaowei
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014074732367.82
175 rdf:type schema:Person
176 sg:pub.10.1007/s00467-015-3134-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034384183
177 https://doi.org/10.1007/s00467-015-3134-2
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/s40620-016-0278-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007203408
180 https://doi.org/10.1007/s40620-016-0278-5
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/cdd.2012.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036434831
183 https://doi.org/10.1038/cdd.2012.63
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/cddis.2013.404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014361079
186 https://doi.org/10.1038/cddis.2013.404
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/cr.2007.78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037118993
189 https://doi.org/10.1038/cr.2007.78
190 rdf:type schema:CreativeWork
191 sg:pub.10.1038/ki.2013.462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001054116
192 https://doi.org/10.1038/ki.2013.462
193 rdf:type schema:CreativeWork
194 sg:pub.10.1038/nature07976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010869485
195 https://doi.org/10.1038/nature07976
196 rdf:type schema:CreativeWork
197 sg:pub.10.1038/nm.3762 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045967711
198 https://doi.org/10.1038/nm.3762
199 rdf:type schema:CreativeWork
200 sg:pub.10.1038/nprot.2013.055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011137998
201 https://doi.org/10.1038/nprot.2013.055
202 rdf:type schema:CreativeWork
203 sg:pub.10.1038/nrneph.2017.129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092056746
204 https://doi.org/10.1038/nrneph.2017.129
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/sj.ki.5001834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034040060
207 https://doi.org/10.1038/sj.ki.5001834
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/srep18944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050914373
210 https://doi.org/10.1038/srep18944
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.bbrc.2009.03.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011153229
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/j.cmet.2011.03.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001967281
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/j.cmet.2017.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083751033
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.cub.2015.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045465245
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.devcel.2015.01.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031444977
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.kint.2015.11.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012362979
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.kint.2016.04.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032627630
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.metabol.2014.02.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008577067
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.tcb.2015.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024005824
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0140-6736(16)32064-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036312804
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1038/embor.2011.260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009399363
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1056/nejmra1205406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033005246
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.0630588100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017297859
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1073/pnas.1409323111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041361316
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1074/jbc.m111.308460 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012009266
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1080/15548627.2015.1124226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033073799
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1080/15548627.2016.1166317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018482225
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1080/15548627.2016.1190071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043022677
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1080/15548627.2017.1341464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091247095
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1093/hmg/ddm100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028741317
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1097/mnh.0b013e32833aa4ac schema:sameAs https://app.dimensions.ai/details/publication/pub.1021175577
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1111/j.1440-1797.2011.01541.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003362659
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1152/ajpendo.1997.273.3.e650 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083154539
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1152/ajprenal.00022.2011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004554230
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1172/jci71863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014075787
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1194/jlr.p040501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026482557
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1371/journal.pmed.0020045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001880244
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1681/asn.2013101068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011511233
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1681/asn.2017030314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092132985
269 rdf:type schema:CreativeWork
270 https://doi.org/10.2337/db15-0473 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070729624
271 rdf:type schema:CreativeWork
272 https://doi.org/10.2353/ajpath.2010.090345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051500112
273 rdf:type schema:CreativeWork
274 https://doi.org/10.2353/ajpath.2010.091012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046443611
275 rdf:type schema:CreativeWork
276 https://doi.org/10.3892/ijmm.2013.1232 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071507470
277 rdf:type schema:CreativeWork
278 https://doi.org/10.4161/auto.22930 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014738825
279 rdf:type schema:CreativeWork
280 https://doi.org/10.4161/auto.24893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006076250
281 rdf:type schema:CreativeWork
282 https://doi.org/10.4161/auto.26563 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072289878
283 rdf:type schema:CreativeWork
284 https://www.grid.ac/institutes/grid.412632.0 schema:alternateName Renmin Hospital of Wuhan University
285 schema:name Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
286 Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China
287 rdf:type schema:Organization
288 https://www.grid.ac/institutes/grid.440771.1 schema:alternateName Hubei University for Nationalities
289 schema:name Department of Nephrology, University Hospital of Hubei University for Nationalities, Enshi, China
290 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...