Ontology type: schema:ScholarlyArticle Open Access: True
2019-09-10
AUTHORSAlejandro Herreros-Pomares, Juan Diego de-Maya-Girones, Silvia Calabuig-Fariñas, Rut Lucas, Alicia Martínez, José Miguel Pardo-Sánchez, Sergio Alonso, Ana Blasco, Ricardo Guijarro, Miguel Martorell, Eva Escorihuela, María Dolores Chiara, Elena Duréndez, Carolina Gandía, Jerónimo Forteza, Rafael Sirera, Eloísa Jantus-Lewintre, Rosa Farràs, Carlos Camps
ABSTRACTThe high resistance against current therapies found in non-small-cell lung cancer (NSCLC) has been associated to cancer stem-like cells (CSCs), a population for which the identification of targets and biomarkers is still under development. In this study, primary cultures from early-stage NSCLC patients were established, using sphere-forming assays for CSC enrichment and adherent conditions for the control counterparts. Patient-derived tumorspheres showed self-renewal and unlimited exponential growth potentials, resistance against chemotherapeutic agents, invasion and differentiation capacities in vitro, and superior tumorigenic potential in vivo. Using quantitative PCR, gene expression profiles were analyzed and NANOG, NOTCH3, CD44, CDKN1A, SNAI1, and ITGA6 were selected to distinguish tumorspheres from adherent cells. Immunoblot and immunofluorescence analyses confirmed that proteins encoded by these genes were consistently increased in tumorspheres from adenocarcinoma patients and showed differential localization and expression patterns. The prognostic role of genes significantly overexpressed in tumorspheres was evaluated in a NSCLC cohort (N = 661) from The Cancer Genome Atlas. Based on a Cox regression analysis, CDKN1A, SNAI1, and ITGA6 were found to be associated with prognosis and used to calculate a gene expression score, named CSC score. Kaplan–Meier survival analysis showed that patients with high CSC score have shorter overall survival (OS) in the entire cohort [37.7 vs. 60.4 months (mo), p = 0.001] and the adenocarcinoma subcohort [36.6 vs. 53.5 mo, p = 0.003], but not in the squamous cell carcinoma one. Multivariate analysis indicated that this gene expression score is an independent biomarker of prognosis for OS in both the entire cohort [hazard ratio (HR): 1.498; 95% confidence interval (CI), 1.167–1.922; p = 0.001] and the adenocarcinoma subcohort [HR: 1.869; 95% CI, 1.275–2.738; p = 0.001]. This score was also analyzed in an independent cohort of 114 adenocarcinoma patients, confirming its prognostic value [42.90 vs. not reached (NR) mo, p = 0.020]. In conclusion, our findings provide relevant prognostic information for lung adenocarcinoma patients and the basis for developing novel therapies. Further studies are required to identify suitable markers and targets for lung squamous cell carcinoma patients. More... »
PAGES660
http://scigraph.springernature.com/pub.10.1038/s41419-019-1898-1
DOIhttp://dx.doi.org/10.1038/s41419-019-1898-1
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1120955211
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/31506430
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "A549 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Adenocarcinoma of Lung",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Adult",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aged",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aged, 80 and over",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carcinoma, Non-Small-Cell Lung",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carcinoma, Squamous Cell",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation, Neoplastic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lung Neoplasms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred NOD",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Middle Aged",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neoplasm Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neoplastic Stem Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Spheroids, Cellular",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "CIBERONC, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.510933.d",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Herreros-Pomares",
"givenName": "Alejandro",
"id": "sg:person.010461346141.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010461346141.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "de-Maya-Girones",
"givenName": "Juan Diego",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Universitat de Val\u00e8ncia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.5338.d",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain",
"Department of Pathology, Universitat de Val\u00e8ncia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Calabuig-Fari\u00f1as",
"givenName": "Silvia",
"id": "sg:person.0630231377.36",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630231377.36"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of History of Science and Documentation, Universitat de Val\u00e8ncia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.5338.d",
"name": [
"Department of History of Science and Documentation, Universitat de Val\u00e8ncia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Lucas",
"givenName": "Rut",
"id": "sg:person.01013410400.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013410400.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Cytomic Core Facility, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Cytomic Core Facility, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Mart\u00ednez",
"givenName": "Alicia",
"id": "sg:person.013661017052.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013661017052.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Pardo-S\u00e1nchez",
"givenName": "Jos\u00e9 Miguel",
"id": "sg:person.012525225500.80",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012525225500.80"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Program of Predictive and Personalized Medicine of Cancer, Institut de Reserca Germans Trias i Pujol (PMPPC-IGTP), Badalona, Spain",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Program of Predictive and Personalized Medicine of Cancer, Institut de Reserca Germans Trias i Pujol (PMPPC-IGTP), Badalona, Spain"
],
"type": "Organization"
},
"familyName": "Alonso",
"givenName": "Sergio",
"id": "sg:person.01240515061.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240515061.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Medical Oncology, Hospital General Universitario de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.106023.6",
"name": [
"Department of Medical Oncology, Hospital General Universitario de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Blasco",
"givenName": "Ana",
"id": "sg:person.0637633671.67",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637633671.67"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Thoracic Surgery, Hospital General Universitario de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.106023.6",
"name": [
"Department of Thoracic Surgery, Hospital General Universitario de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Guijarro",
"givenName": "Ricardo",
"id": "sg:person.01163156033.70",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163156033.70"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Hospital General Universitario de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.106023.6",
"name": [
"Department of Pathology, Hospital General Universitario de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Martorell",
"givenName": "Miguel",
"id": "sg:person.0674510553.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674510553.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CIBERONC, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.510933.d",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Escorihuela",
"givenName": "Eva",
"id": "sg:person.015540377752.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015540377752.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Sanitary Research of Asturias, Hospital Central de Asturias, Universidad de Oviedo, Oviedo, Spain",
"id": "http://www.grid.ac/institutes/None",
"name": [
"CIBERONC, Valencia, Spain",
"Institute of Sanitary Research of Asturias, Hospital Central de Asturias, Universidad de Oviedo, Oviedo, Spain"
],
"type": "Organization"
},
"familyName": "Chiara",
"givenName": "Mar\u00eda Dolores",
"id": "sg:person.01225314417.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225314417.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CIBERONC, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.510933.d",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Dur\u00e9ndez",
"givenName": "Elena",
"id": "sg:person.010137552720.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010137552720.64"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Gand\u00eda",
"givenName": "Carolina",
"id": "sg:person.0700311024.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700311024.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Instituto Valenciano de Patolog\u00eda, Unidad Mixta de Patolog\u00eda Molecular, Centro Investigaci\u00f3n Pr\u00edncipe Felipe/Universidad Cat\u00f3lica de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Instituto Valenciano de Patolog\u00eda, Unidad Mixta de Patolog\u00eda Molecular, Centro Investigaci\u00f3n Pr\u00edncipe Felipe/Universidad Cat\u00f3lica de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Forteza",
"givenName": "Jer\u00f3nimo",
"id": "sg:person.0723524314.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723524314.03"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biotechnology, Universidad Polit\u00e9cnica de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.157927.f",
"name": [
"CIBERONC, Valencia, Spain",
"Department of Biotechnology, Universidad Polit\u00e9cnica de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Sirera",
"givenName": "Rafael",
"id": "sg:person.01170443474.79",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170443474.79"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biotechnology, Universidad Polit\u00e9cnica de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.157927.f",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain",
"Department of Biotechnology, Universidad Polit\u00e9cnica de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Jantus-Lewintre",
"givenName": "Elo\u00edsa",
"id": "sg:person.01363575715.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363575715.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.418274.c",
"name": [
"Oncogenic Signalling Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Farr\u00e0s",
"givenName": "Rosa",
"id": "sg:person.01123323545.67",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123323545.67"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Medicine, Hospital General Universitario de Valencia, Valencia, Spain",
"id": "http://www.grid.ac/institutes/grid.106023.6",
"name": [
"Molecular Oncology Laboratory, Fundaci\u00f3n Hospital General Universitario de Valencia, Valencia, Spain",
"CIBERONC, Valencia, Spain",
"Department of Medical Oncology, Hospital General Universitario de Valencia, Valencia, Spain",
"Department of Medicine, Hospital General Universitario de Valencia, Valencia, Spain"
],
"type": "Organization"
},
"familyName": "Camps",
"givenName": "Carlos",
"id": "sg:person.01350463315.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350463315.58"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nature13385",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038580641",
"https://doi.org/10.1038/nature13385"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2011.170",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008939235",
"https://doi.org/10.1038/cdd.2011.170"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc1590",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050924893",
"https://doi.org/10.1038/nrc1590"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature25183",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1100588738",
"https://doi.org/10.1038/nature25183"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc2657",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031760843",
"https://doi.org/10.1038/nrc2657"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrclinonc.2015.61",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050085746",
"https://doi.org/10.1038/nrclinonc.2015.61"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.2478/s11658-013-0112-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031385542",
"https://doi.org/10.2478/s11658-013-0112-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2014.105",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028978045",
"https://doi.org/10.1038/cdd.2014.105"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng1167",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047743086",
"https://doi.org/10.1038/ng1167"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-017-09929-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091344526",
"https://doi.org/10.1038/s41598-017-09929-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.cdd.4402283",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043286075",
"https://doi.org/10.1038/sj.cdd.4402283"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2014.156",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028213905",
"https://doi.org/10.1038/cdd.2014.156"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncb2717",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006990543",
"https://doi.org/10.1038/ncb2717"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12885-015-1086-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024019889",
"https://doi.org/10.1186/s12885-015-1086-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature05384",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026901039",
"https://doi.org/10.1038/nature05384"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1245/s10434-013-3330-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035156844",
"https://doi.org/10.1245/s10434-013-3330-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature11404",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006014163",
"https://doi.org/10.1038/nature11404"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-09-10",
"datePublishedReg": "2019-09-10",
"description": "The high resistance against current therapies found in non-small-cell lung cancer (NSCLC) has been associated to cancer stem-like cells (CSCs), a population for which the identification of targets and biomarkers is still under development. In this study, primary cultures from early-stage NSCLC patients were established, using sphere-forming assays for CSC enrichment and adherent conditions for the control counterparts. Patient-derived tumorspheres showed self-renewal and unlimited exponential growth potentials, resistance against chemotherapeutic agents, invasion and differentiation capacities in vitro, and superior tumorigenic potential in vivo. Using quantitative PCR, gene expression profiles were analyzed and NANOG, NOTCH3, CD44, CDKN1A, SNAI1, and ITGA6 were selected to distinguish tumorspheres from adherent cells. Immunoblot and immunofluorescence analyses confirmed that proteins encoded by these genes were consistently increased in tumorspheres from adenocarcinoma patients and showed differential localization and expression patterns. The prognostic role of genes significantly overexpressed in tumorspheres was evaluated in a NSCLC cohort (N\u2009=\u2009661) from The Cancer Genome Atlas. Based on a Cox regression analysis, CDKN1A, SNAI1, and ITGA6 were found to be associated with prognosis and used to calculate a gene expression score, named CSC score. Kaplan\u2013Meier survival analysis showed that patients with high CSC score have shorter overall survival (OS) in the entire cohort [37.7 vs. 60.4 months (mo), p\u2009=\u20090.001] and the adenocarcinoma subcohort [36.6 vs. 53.5 mo, p\u2009=\u20090.003], but not in the squamous cell carcinoma one. Multivariate analysis indicated that this gene expression score is an independent biomarker of prognosis for OS in both the entire cohort [hazard ratio (HR): 1.498; 95% confidence interval (CI), 1.167\u20131.922; p\u2009=\u20090.001] and the adenocarcinoma subcohort [HR: 1.869; 95% CI, 1.275\u20132.738; p\u2009=\u20090.001]. This score was also analyzed in an independent cohort of 114 adenocarcinoma patients, confirming its prognostic value [42.90 vs. not reached (NR) mo, p\u2009=\u20090.020]. In conclusion, our findings provide relevant prognostic information for lung adenocarcinoma patients and the basis for developing novel therapies. Further studies are required to identify suitable markers and targets for lung squamous cell carcinoma patients.",
"genre": "article",
"id": "sg:pub.10.1038/s41419-019-1898-1",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.8098081",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.8098180",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1042939",
"issn": [
"2041-4889"
],
"name": "Cell Death & Disease",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "9",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "10"
}
],
"keywords": [
"cell lung cancer",
"cancer stem-like cells",
"overall survival",
"adenocarcinoma patients",
"gene expression score",
"entire cohort",
"lung cancer",
"lung squamous cell carcinoma patients",
"expression score",
"squamous cell carcinoma patients",
"early-stage NSCLC patients",
"Kaplan-Meier survival analysis",
"cancer stem cell-like properties",
"CSC score",
"cell carcinoma patients",
"Cox regression analysis",
"shorter overall survival",
"lung adenocarcinoma patients",
"relevant prognostic information",
"sphere-forming assays",
"stem cell-like properties",
"cell-like properties",
"stem-like cells",
"lung tumorspheres",
"NSCLC cohort",
"NSCLC patients",
"prognostic role",
"Cancer Genome Atlas",
"prognostic impact",
"carcinoma patients",
"prognostic value",
"independent biomarker",
"current therapies",
"prognostic information",
"novel therapies",
"gene expression profiles",
"independent cohort",
"patients",
"survival analysis",
"multivariate analysis",
"chemotherapeutic agents",
"cohort",
"primary cultures",
"CSC enrichment",
"tumorspheres",
"Genome Atlas",
"tumorigenic potential",
"identification of targets",
"expression patterns",
"immunofluorescence analysis",
"subcohort",
"expression profiles",
"Further studies",
"scores",
"control counterparts",
"regression analysis",
"prognosis",
"therapy",
"differentiation capacity",
"cancer",
"quantitative PCR",
"differential localization",
"adherent conditions",
"biomarkers",
"suitable marker",
"SNAI1",
"ITGA6",
"adherent cells",
"genes",
"CDKN1A",
"cells",
"Notch3",
"CD44",
"survival",
"target",
"immunoblot",
"growth potential",
"vivo",
"Nanog",
"invasion",
"markers",
"study",
"PCR",
"protein",
"conclusion",
"assays",
"resistance",
"population",
"high resistance",
"agents",
"findings",
"atlas",
"analysis",
"localization",
"enrichment",
"role",
"potential",
"identification",
"profile",
"culture",
"patterns",
"development",
"impact",
"capacity",
"basis",
"counterparts",
"information",
"values",
"conditions",
"one",
"properties"
],
"name": "Lung tumorspheres reveal cancer stem cell-like properties and a score with prognostic impact in resected non-small-cell lung cancer",
"pagination": "660",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1120955211"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41419-019-1898-1"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"31506430"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41419-019-1898-1",
"https://app.dimensions.ai/details/publication/pub.1120955211"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:36",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_814.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41419-019-1898-1"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41419-019-1898-1'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41419-019-1898-1'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41419-019-1898-1'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41419-019-1898-1'
This table displays all metadata directly associated to this object as RDF triples.
482 TRIPLES
22 PREDICATES
173 URIs
148 LITERALS
26 BLANK NODES