Bok regulates mitochondrial fusion and morphology. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04-11

AUTHORS

Jacqualyn J Schulman, Laura M Szczesniak, Eric N Bunker, Heather A Nelson, Michael W Roe, Larry E Wagner, David I Yule, Richard J H Wojcikiewicz

ABSTRACT

Bok (Bcl-2-related ovarian killer) is a member of the Bcl-2 protein family that governs the intrinsic apoptosis pathway, but the cellular role that Bok plays is controversial. Remarkably, endogenous Bok is constitutively bound to inositol 1,4,5-trisphosphate receptors (IP3Rs) and is stabilized by this interaction. Here we report that despite the strong association with IP3Rs, deletion of Bok expression by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease)-mediated gene editing does not alter calcium mobilization via IP3Rs or calcium influx into the mitochondria. Rather, Bok deletion significantly reduces mitochondrial fusion rate, resulting in mitochondrial fragmentation. This phenotype is reversed by exogenous wild-type Bok and by an IP3R binding-deficient Bok mutant, and may result from a decrease in mitochondrial motility. Bok deletion also enhances mitochondrial spare respiratory capacity and membrane potential. Finally, Bok does not play a major role in apoptotic signaling, since Bok deletion does not alter responsiveness to various apoptotic stimuli. Overall, despite binding to IP3Rs, Bok does not alter IP3R-mediated Ca2+ signaling, but is required to maintain normal mitochondrial fusion, morphology, and bioenergetics. More... »

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41418-019-0327-4

DOI

http://dx.doi.org/10.1038/s41418-019-0327-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113378373

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30976095


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "SUNY Upstate Medical University", 
          "id": "https://www.grid.ac/institutes/grid.411023.5", 
          "name": [
            "Department of Pharmacology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY, 13210, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schulman", 
        "givenName": "Jacqualyn J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "SUNY Upstate Medical University", 
          "id": "https://www.grid.ac/institutes/grid.411023.5", 
          "name": [
            "Department of Pharmacology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY, 13210, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Szczesniak", 
        "givenName": "Laura M", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Neurological Disorders and Stroke", 
          "id": "https://www.grid.ac/institutes/grid.416870.c", 
          "name": [
            "Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bunker", 
        "givenName": "Eric N", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "SUNY Upstate Medical University", 
          "id": "https://www.grid.ac/institutes/grid.411023.5", 
          "name": [
            "Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nelson", 
        "givenName": "Heather A", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "SUNY Upstate Medical University", 
          "id": "https://www.grid.ac/institutes/grid.411023.5", 
          "name": [
            "Department of Cell and Developmental Biology, Department of Medicine, SUNY Upstate Medical University, Syracuse, NY, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roe", 
        "givenName": "Michael W", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Rochester", 
          "id": "https://www.grid.ac/institutes/grid.16416.34", 
          "name": [
            "Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wagner", 
        "givenName": "Larry E", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Rochester", 
          "id": "https://www.grid.ac/institutes/grid.16416.34", 
          "name": [
            "Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yule", 
        "givenName": "David I", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "SUNY Upstate Medical University", 
          "id": "https://www.grid.ac/institutes/grid.411023.5", 
          "name": [
            "Department of Pharmacology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY, 13210, USA. Wojcikir@upstate.edu."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wojcikiewicz", 
        "givenName": "Richard J H", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.ceca.2011.05.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001787845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m206607200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002332412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-genet-110410-132529", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002454426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006266667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2010.89", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007824301", 
          "https://doi.org/10.1038/cdd.2010.89"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.3780-15.2016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007981076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200809060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008110321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008703730", 
          "https://doi.org/10.1038/nmeth.2649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009398987", 
          "https://doi.org/10.1038/nature05111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009398987", 
          "https://doi.org/10.1038/nature05111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009398987", 
          "https://doi.org/10.1038/nature05111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.2012.228320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011625996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m115.711242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011728259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1521979113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012690394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.46.30139", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012915431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m701862200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014198402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.19.11678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015519326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2013.143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016534425", 
          "https://doi.org/10.1038/nprot.2013.143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.23.12401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016957673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00204-014-1448-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016987582", 
          "https://doi.org/10.1007/s00204-014-1448-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1516347113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017264661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2016.09.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017402048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2015.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022116979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.06.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023729243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.06.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023729243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e15-01-0028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025439802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.11.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025644754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.11.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025644754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m113.496570", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026052519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026857432", 
          "https://doi.org/10.1038/ncb2488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.02.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028054185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.02.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028054185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbabio.2009.03.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029012884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20040072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030341208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20040072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030341208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbcan.2012.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033235103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1421063112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035440087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2003.09.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039049019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature15249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042095619", 
          "https://doi.org/10.1038/nature15249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0703976104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042856144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/febs.14008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046362680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2011.210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047922330", 
          "https://doi.org/10.1038/cdd.2011.210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2013.10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048470552", 
          "https://doi.org/10.1038/cdd.2013.10"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1471-4159.1994.63010177.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048606765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m507681200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048709729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m507681200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048709729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90430-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049033835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.193946", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049880080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.00035.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050356380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8235595", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062653729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/stke.3532006pl6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062693447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076951318", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079981956", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2017.03.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084098452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2017.04.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085121916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2017.179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092311170", 
          "https://doi.org/10.1038/cdd.2017.179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2017.186", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092747563", 
          "https://doi.org/10.1038/cdd.2017.186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2018.04.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103995941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2018.04.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103995941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2018.04.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103995941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2018.04.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103995941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2018.04.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103995941"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-04-11", 
    "datePublishedReg": "2019-04-11", 
    "description": "Bok (Bcl-2-related ovarian killer) is a member of the Bcl-2 protein family that governs the intrinsic apoptosis pathway, but the cellular role that Bok plays is controversial. Remarkably, endogenous Bok is constitutively bound to inositol 1,4,5-trisphosphate receptors (IP3Rs) and is stabilized by this interaction. Here we report that despite the strong association with IP3Rs, deletion of Bok expression by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease)-mediated gene editing does not alter calcium mobilization via IP3Rs or calcium influx into the mitochondria. Rather, Bok deletion significantly reduces mitochondrial fusion rate, resulting in mitochondrial fragmentation. This phenotype is reversed by exogenous wild-type Bok and by an IP3R binding-deficient Bok mutant, and may result from a decrease in mitochondrial motility. Bok deletion also enhances mitochondrial spare respiratory capacity and membrane potential. Finally, Bok does not play a major role in apoptotic signaling, since Bok deletion does not alter responsiveness to various apoptotic stimuli. Overall, despite binding to IP3Rs, Bok does not alter IP3R-mediated Ca2+ signaling, but is required to maintain normal mitochondrial fusion, morphology, and bioenergetics.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41418-019-0327-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6952717", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7027457", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1112551", 
        "issn": [
          "1350-9047", 
          "1476-5403"
        ], 
        "name": "Cell Death & Differentiation", 
        "type": "Periodical"
      }
    ], 
    "name": "Bok regulates mitochondrial fusion and morphology.", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41418-019-0327-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113378373"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9437445"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30976095"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41418-019-0327-4", 
      "https://app.dimensions.ai/details/publication/pub.1113378373"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-16T06:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000377_0000000377/records_106825_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/s41418-019-0327-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41418-019-0327-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41418-019-0327-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41418-019-0327-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41418-019-0327-4'


 

This table displays all metadata directly associated to this object as RDF triples.

274 TRIPLES      20 PREDICATES      75 URIs      16 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41418-019-0327-4 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N1e6971825c3943ec8153faf3322b952e
4 schema:citation sg:pub.10.1007/s00204-014-1448-7
5 sg:pub.10.1038/cdd.2010.89
6 sg:pub.10.1038/cdd.2011.210
7 sg:pub.10.1038/cdd.2013.10
8 sg:pub.10.1038/cdd.2017.179
9 sg:pub.10.1038/cdd.2017.186
10 sg:pub.10.1038/nature05111
11 sg:pub.10.1038/nature15249
12 sg:pub.10.1038/ncb2488
13 sg:pub.10.1038/nmeth.2649
14 sg:pub.10.1038/nprot.2013.143
15 https://app.dimensions.ai/details/publication/pub.1076951318
16 https://app.dimensions.ai/details/publication/pub.1079981956
17 https://doi.org/10.1016/0092-8674(95)90430-1
18 https://doi.org/10.1016/j.bbabio.2009.03.015
19 https://doi.org/10.1016/j.bbamcr.2003.09.006
20 https://doi.org/10.1016/j.bbcan.2012.12.001
21 https://doi.org/10.1016/j.bbrc.2015.02.004
22 https://doi.org/10.1016/j.ceca.2011.05.011
23 https://doi.org/10.1016/j.cell.2016.02.026
24 https://doi.org/10.1016/j.celrep.2018.04.060
25 https://doi.org/10.1016/j.devcel.2007.11.019
26 https://doi.org/10.1016/j.molcel.2010.06.029
27 https://doi.org/10.1016/j.molcel.2010.11.030
28 https://doi.org/10.1016/j.neuint.2017.03.010
29 https://doi.org/10.1016/j.neuint.2017.04.009
30 https://doi.org/10.1016/j.tibs.2016.09.001
31 https://doi.org/10.1042/bj20040072
32 https://doi.org/10.1046/j.1471-4159.1994.63010177.x
33 https://doi.org/10.1073/pnas.0703976104
34 https://doi.org/10.1073/pnas.1421063112
35 https://doi.org/10.1073/pnas.1516347113
36 https://doi.org/10.1073/pnas.1521979113
37 https://doi.org/10.1073/pnas.94.23.12401
38 https://doi.org/10.1074/jbc.270.19.11678
39 https://doi.org/10.1074/jbc.273.46.30139
40 https://doi.org/10.1074/jbc.m113.496570
41 https://doi.org/10.1074/jbc.m115.711242
42 https://doi.org/10.1074/jbc.m206607200
43 https://doi.org/10.1074/jbc.m507681200
44 https://doi.org/10.1074/jbc.m701862200
45 https://doi.org/10.1083/jcb.200809060
46 https://doi.org/10.1091/mbc.e15-01-0028
47 https://doi.org/10.1111/febs.14008
48 https://doi.org/10.1113/jphysiol.2012.228320
49 https://doi.org/10.1126/science.8235595
50 https://doi.org/10.1126/stke.3532006pl6
51 https://doi.org/10.1146/annurev-genet-110410-132529
52 https://doi.org/10.1152/physrev.00035.2006
53 https://doi.org/10.1242/jcs.193946
54 https://doi.org/10.1523/jneurosci.3780-15.2016
55 schema:datePublished 2019-04-11
56 schema:datePublishedReg 2019-04-11
57 schema:description Bok (Bcl-2-related ovarian killer) is a member of the Bcl-2 protein family that governs the intrinsic apoptosis pathway, but the cellular role that Bok plays is controversial. Remarkably, endogenous Bok is constitutively bound to inositol 1,4,5-trisphosphate receptors (IP<sub>3</sub>Rs) and is stabilized by this interaction. Here we report that despite the strong association with IP<sub>3</sub>Rs, deletion of Bok expression by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease)-mediated gene editing does not alter calcium mobilization via IP<sub>3</sub>Rs or calcium influx into the mitochondria. Rather, Bok deletion significantly reduces mitochondrial fusion rate, resulting in mitochondrial fragmentation. This phenotype is reversed by exogenous wild-type Bok and by an IP<sub>3</sub>R binding-deficient Bok mutant, and may result from a decrease in mitochondrial motility. Bok deletion also enhances mitochondrial spare respiratory capacity and membrane potential. Finally, Bok does not play a major role in apoptotic signaling, since Bok deletion does not alter responsiveness to various apoptotic stimuli. Overall, despite binding to IP<sub>3</sub>Rs, Bok does not alter IP<sub>3</sub>R-mediated Ca<sup>2+</sup> signaling, but is required to maintain normal mitochondrial fusion, morphology, and bioenergetics.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf sg:journal.1112551
62 schema:name Bok regulates mitochondrial fusion and morphology.
63 schema:productId Nb626a353ab8e4b4fa020e9e2377d044f
64 Nc17280b8397a4c7eb0a6904c38e71d94
65 Nc54db1151a0c44678a6e655b7b40f106
66 Nf94db77831c74b62a3d5337272837bf9
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113378373
68 https://doi.org/10.1038/s41418-019-0327-4
69 schema:sdDatePublished 2019-04-16T06:23
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher N6a12aa7ee9de4cc999e04669256b0fa9
72 schema:url http://www.nature.com/articles/s41418-019-0327-4
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N1e6971825c3943ec8153faf3322b952e rdf:first Na505e8c2484e43d3b329d11351d88666
77 rdf:rest N2972a6deca9546dd9fcb0b36ec9ce324
78 N2972a6deca9546dd9fcb0b36ec9ce324 rdf:first N8b3a8a0ba9cd4a06aa0f3297454e1a11
79 rdf:rest N7604e64bc56c4ea58d124e8eeee8eb1b
80 N6a12aa7ee9de4cc999e04669256b0fa9 schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 N7604e64bc56c4ea58d124e8eeee8eb1b rdf:first Nedec1196651c429c939ab993fe4e09e8
83 rdf:rest N9b2253f9eee64daab4bd131af8ce1e17
84 N762dd2bda5f844ef8ddff690f120d360 schema:affiliation https://www.grid.ac/institutes/grid.411023.5
85 schema:familyName Nelson
86 schema:givenName Heather A
87 rdf:type schema:Person
88 N7ceac51d25734395ad87cbd2892c5e47 schema:affiliation https://www.grid.ac/institutes/grid.411023.5
89 schema:familyName Roe
90 schema:givenName Michael W
91 rdf:type schema:Person
92 N8b3a8a0ba9cd4a06aa0f3297454e1a11 schema:affiliation https://www.grid.ac/institutes/grid.411023.5
93 schema:familyName Szczesniak
94 schema:givenName Laura M
95 rdf:type schema:Person
96 N9247c41cdf8f42c28ab0739d56589b61 rdf:first N7ceac51d25734395ad87cbd2892c5e47
97 rdf:rest Nb447cf5dcbd3405b9f97363b0e6d75c9
98 N93de33c2dcb542a285f30406939ca872 schema:affiliation https://www.grid.ac/institutes/grid.16416.34
99 schema:familyName Yule
100 schema:givenName David I
101 rdf:type schema:Person
102 N9b2253f9eee64daab4bd131af8ce1e17 rdf:first N762dd2bda5f844ef8ddff690f120d360
103 rdf:rest N9247c41cdf8f42c28ab0739d56589b61
104 Na505e8c2484e43d3b329d11351d88666 schema:affiliation https://www.grid.ac/institutes/grid.411023.5
105 schema:familyName Schulman
106 schema:givenName Jacqualyn J
107 rdf:type schema:Person
108 Na5e6480c935749d4a2cce072aa7a2619 rdf:first N93de33c2dcb542a285f30406939ca872
109 rdf:rest Nb35a921e8cfc4b869f3aa612257f65a2
110 Nb35a921e8cfc4b869f3aa612257f65a2 rdf:first Nfcd15d44a4e2473ab764950d965c5fa8
111 rdf:rest rdf:nil
112 Nb447cf5dcbd3405b9f97363b0e6d75c9 rdf:first Ne75e65ea6b9d46459def822d3b8ca485
113 rdf:rest Na5e6480c935749d4a2cce072aa7a2619
114 Nb626a353ab8e4b4fa020e9e2377d044f schema:name dimensions_id
115 schema:value pub.1113378373
116 rdf:type schema:PropertyValue
117 Nc17280b8397a4c7eb0a6904c38e71d94 schema:name doi
118 schema:value 10.1038/s41418-019-0327-4
119 rdf:type schema:PropertyValue
120 Nc54db1151a0c44678a6e655b7b40f106 schema:name nlm_unique_id
121 schema:value 9437445
122 rdf:type schema:PropertyValue
123 Ne75e65ea6b9d46459def822d3b8ca485 schema:affiliation https://www.grid.ac/institutes/grid.16416.34
124 schema:familyName Wagner
125 schema:givenName Larry E
126 rdf:type schema:Person
127 Nedec1196651c429c939ab993fe4e09e8 schema:affiliation https://www.grid.ac/institutes/grid.416870.c
128 schema:familyName Bunker
129 schema:givenName Eric N
130 rdf:type schema:Person
131 Nf94db77831c74b62a3d5337272837bf9 schema:name pubmed_id
132 schema:value 30976095
133 rdf:type schema:PropertyValue
134 Nfcd15d44a4e2473ab764950d965c5fa8 schema:affiliation https://www.grid.ac/institutes/grid.411023.5
135 schema:familyName Wojcikiewicz
136 schema:givenName Richard J H
137 rdf:type schema:Person
138 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biological Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biochemistry and Cell Biology
143 rdf:type schema:DefinedTerm
144 sg:grant.6952717 http://pending.schema.org/fundedItem sg:pub.10.1038/s41418-019-0327-4
145 rdf:type schema:MonetaryGrant
146 sg:grant.7027457 http://pending.schema.org/fundedItem sg:pub.10.1038/s41418-019-0327-4
147 rdf:type schema:MonetaryGrant
148 sg:journal.1112551 schema:issn 1350-9047
149 1476-5403
150 schema:name Cell Death & Differentiation
151 rdf:type schema:Periodical
152 sg:pub.10.1007/s00204-014-1448-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016987582
153 https://doi.org/10.1007/s00204-014-1448-7
154 rdf:type schema:CreativeWork
155 sg:pub.10.1038/cdd.2010.89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007824301
156 https://doi.org/10.1038/cdd.2010.89
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/cdd.2011.210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047922330
159 https://doi.org/10.1038/cdd.2011.210
160 rdf:type schema:CreativeWork
161 sg:pub.10.1038/cdd.2013.10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048470552
162 https://doi.org/10.1038/cdd.2013.10
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/cdd.2017.179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092311170
165 https://doi.org/10.1038/cdd.2017.179
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/cdd.2017.186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092747563
168 https://doi.org/10.1038/cdd.2017.186
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/nature05111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009398987
171 https://doi.org/10.1038/nature05111
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/nature15249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042095619
174 https://doi.org/10.1038/nature15249
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/ncb2488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026857432
177 https://doi.org/10.1038/ncb2488
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/nmeth.2649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008703730
180 https://doi.org/10.1038/nmeth.2649
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nprot.2013.143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016534425
183 https://doi.org/10.1038/nprot.2013.143
184 rdf:type schema:CreativeWork
185 https://app.dimensions.ai/details/publication/pub.1076951318 schema:CreativeWork
186 https://app.dimensions.ai/details/publication/pub.1079981956 schema:CreativeWork
187 https://doi.org/10.1016/0092-8674(95)90430-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049033835
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.bbabio.2009.03.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029012884
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.bbamcr.2003.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039049019
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.bbcan.2012.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033235103
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.bbrc.2015.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022116979
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.ceca.2011.05.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001787845
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.cell.2016.02.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028054185
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.celrep.2018.04.060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103995941
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.devcel.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006266667
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.molcel.2010.06.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023729243
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.molcel.2010.11.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025644754
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.neuint.2017.03.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084098452
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/j.neuint.2017.04.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085121916
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.tibs.2016.09.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017402048
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1042/bj20040072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030341208
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1046/j.1471-4159.1994.63010177.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048606765
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1073/pnas.0703976104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042856144
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1073/pnas.1421063112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035440087
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1073/pnas.1516347113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017264661
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.1521979113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012690394
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.94.23.12401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016957673
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1074/jbc.270.19.11678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015519326
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1074/jbc.273.46.30139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012915431
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/jbc.m113.496570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026052519
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1074/jbc.m115.711242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011728259
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1074/jbc.m206607200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002332412
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1074/jbc.m507681200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048709729
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1074/jbc.m701862200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014198402
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1083/jcb.200809060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008110321
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1091/mbc.e15-01-0028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025439802
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1111/febs.14008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046362680
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1113/jphysiol.2012.228320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011625996
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1126/science.8235595 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062653729
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1126/stke.3532006pl6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062693447
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1146/annurev-genet-110410-132529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002454426
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1152/physrev.00035.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050356380
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1242/jcs.193946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049880080
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1523/jneurosci.3780-15.2016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007981076
262 rdf:type schema:CreativeWork
263 https://www.grid.ac/institutes/grid.16416.34 schema:alternateName University of Rochester
264 schema:name Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA.
265 rdf:type schema:Organization
266 https://www.grid.ac/institutes/grid.411023.5 schema:alternateName SUNY Upstate Medical University
267 schema:name Department of Cell and Developmental Biology, Department of Medicine, SUNY Upstate Medical University, Syracuse, NY, USA.
268 Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY, USA.
269 Department of Pharmacology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY, 13210, USA.
270 Department of Pharmacology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY, 13210, USA. Wojcikir@upstate.edu.
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.416870.c schema:alternateName National Institute of Neurological Disorders and Stroke
273 schema:name Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
274 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...