Single cell fluorescence imaging of glycan uptake by intestinal bacteria View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04-01

AUTHORS

Jan-Hendrik Hehemann, Greta Reintjes, Leeann Klassen, Adam D. Smith, Didier Ndeh, Carol Arnosti, Rudolf Amann, D. Wade Abbott

ABSTRACT

Microbes in the intestines of mammals degrade dietary glycans for energy and growth. The pathways required for polysaccharide utilization are functionally diverse; moreover, they are unequally dispersed between bacterial genomes. Hence, assigning metabolic phenotypes to genotypes remains a challenge in microbiome research. Here we demonstrate that glycan uptake in gut bacteria can be visualized with fluorescent glycan conjugates (FGCs) using epifluorescence microscopy. Yeast α-mannan and rhamnogalacturonan-II, two structurally distinct glycans from the cell walls of yeast and plants, respectively, were fluorescently labeled and fed to Bacteroides thetaiotaomicron VPI-5482. Wild-type cells rapidly consumed the FGCs and became fluorescent; whereas, strains that had deleted pathways for glycan degradation and transport were non-fluorescent. Uptake of FGCs, therefore, is direct evidence of genetic function and provides a direct method to assess specific glycan metabolism in intestinal bacteria at the single cell level. More... »

PAGES

1-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41396-019-0406-z

DOI

http://dx.doi.org/10.1038/s41396-019-0406-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113176799

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30936421


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