Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-07-10

AUTHORS

Sangita Ganesh, Anthony D. Bertagnolli, Laura A. Bristow, Cory C. Padilla, Nigel Blackwood, Montserrat Aldunate, Annie Bourbonnais, Mark A. Altabet, Rex R. Malmstrom, Tanja Woyke, Osvaldo Ulloa, Konstantinos T. Konstantinidis, Bo Thamdrup, Frank J. Stewart

ABSTRACT

Anaerobic ammonium oxidation (anammox) contributes substantially to ocean nitrogen loss, particularly in anoxic marine zones (AMZs). Ammonium is scarce in AMZs, raising the hypothesis that organic nitrogen compounds may be ammonium sources for anammox. Biochemical measurements suggest that the organic compounds urea and cyanate can support anammox in AMZs. However, it is unclear if anammox bacteria degrade these compounds to ammonium themselves, or rely on other organisms for this process. Genes for urea degradation have not been found in anammox bacteria, and genomic evidence for cyanate use for anammox is limited to a cyanase gene recovered from the sediment bacterium Candidatus Scalindua profunda. Here, analysis of Ca. Scalindua single amplified genomes from the Eastern Tropical North Pacific AMZ revealed genes for urea degradation and transport, as well as for cyanate degradation. Urease and cyanase genes were transcribed, along with anammox genes, in the AMZ core where anammox rates peaked. Homologs of these genes were also detected in meta-omic datasets from major AMZs in the Eastern Tropical South Pacific and Arabian Sea. These results suggest that anammox bacteria from different ocean regions can directly access organic nitrogen substrates. Future studies should assess if and under what environmental conditions these substrates contribute to the ammonium budget for anammox. More... »

PAGES

2706-2722

References to SciGraph publications

  • 2014-01-23. Single-cell enabled comparative genomics of a deep ocean SAR11 bathytype in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2009-09-03. Denitrification as the dominant nitrogen loss process in the Arabian Sea in NATURE
  • 2011-12-15. Nitrite oxidation in the Namibian oxygen minimum zone in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2015-07-29. Cyanate as energy source for nitrifiers in NATURE
  • 2015-04-07. Size-fraction partitioning of community gene transcription and nitrogen metabolism in a marine oxygen minimum zone in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-02-26. NC10 bacteria in marine oxygen minimum zones in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2014-04-10. Obtaining genomes from uncultivated environmental microorganisms using FACS–based single-cell genomics in NATURE PROTOCOLS
  • 2017-01-10. Draft Genome of Scalindua rubra, Obtained from the Interface Above the Discovery Deep Brine in the Red Sea, Sheds Light on Potential Salt Adaptation Strategies in Anammox Bacteria in MICROBIAL ECOLOGY
  • 2003-08-13. Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation in NATURE
  • 2013-07-14. Insights into the phylogeny and coding potential of microbial dark matter in NATURE
  • 2016-08-03. SAR11 bacteria linked to ocean anoxia and nitrogen loss in NATURE
  • 2013-02-24. Nitrogen cycling driven by organic matter export in the South Pacific oxygen minimum zone in NATURE GEOSCIENCE
  • 2005-06-28. Availability of urea to autotrophic ammonia-oxidizing bacteria as related to the fate of 14C- and 15N-labeled urea added to soil in BIOLOGY AND FERTILITY OF SOILS
  • 2011-10-02. Molecular mechanism of anaerobic ammonium oxidation in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41396-018-0223-9

    DOI

    http://dx.doi.org/10.1038/s41396-018-0223-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1105465332

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29991764


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ammonium Compounds", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anaerobiosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carbon-Nitrogen Lyases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genomics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nitrogen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oceans and Seas", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oxidation-Reduction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Seawater", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Single-Cell Analysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Urease", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Radiant Genomics, 94608, Emeryville, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.451178.e", 
              "name": [
                "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA", 
                "Radiant Genomics, 94608, Emeryville, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ganesh", 
            "givenName": "Sangita", 
            "id": "sg:person.0715160621.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715160621.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA", 
              "id": "http://www.grid.ac/institutes/grid.213917.f", 
              "name": [
                "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bertagnolli", 
            "givenName": "Anthony D.", 
            "id": "sg:person.01111772272.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111772272.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biogeochemistry Group, Max Planck Institute for Marine Microbiology, Bremen, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419529.2", 
              "name": [
                "Biogeochemistry Group, Max Planck Institute for Marine Microbiology, Bremen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bristow", 
            "givenName": "Laura A.", 
            "id": "sg:person.0751537347.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751537347.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA", 
              "id": "http://www.grid.ac/institutes/grid.213917.f", 
              "name": [
                "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Padilla", 
            "givenName": "Cory C.", 
            "id": "sg:person.0647045421.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647045421.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Blackwood", 
            "givenName": "Nigel", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Departamento de Oceanograf\u00eda, Universidad de Concepci\u00f3n, Casilla 160-C, 4070386, Concepci\u00f3n, Chile", 
              "id": "http://www.grid.ac/institutes/grid.5380.e", 
              "name": [
                "Graduate Program in Oceanography, Department of Oceanography, Faculty of Natural Sciences and Oceanography, University of Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n, Chile", 
                "Departamento de Oceanograf\u00eda, Universidad de Concepci\u00f3n, Casilla 160-C, 4070386, Concepci\u00f3n, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aldunate", 
            "givenName": "Montserrat", 
            "id": "sg:person.012242753633.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012242753633.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA", 
              "id": "http://www.grid.ac/institutes/grid.266686.a", 
              "name": [
                "Marine Chemistry & Geochemistry, Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543, Woods Hole, MA, USA", 
                "School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bourbonnais", 
            "givenName": "Annie", 
            "id": "sg:person.0736172076.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736172076.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA", 
              "id": "http://www.grid.ac/institutes/grid.266686.a", 
              "name": [
                "School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Altabet", 
            "givenName": "Mark A.", 
            "id": "sg:person.0627543264.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627543264.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.451309.a", 
              "name": [
                "Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Malmstrom", 
            "givenName": "Rex R.", 
            "id": "sg:person.01046703646.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046703646.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.451309.a", 
              "name": [
                "Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Woyke", 
            "givenName": "Tanja", 
            "id": "sg:person.01366555074.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366555074.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Departamento de Oceanograf\u00eda, Universidad de Concepci\u00f3n, Casilla 160-C, 4070386, Concepci\u00f3n, Chile", 
              "id": "http://www.grid.ac/institutes/grid.5380.e", 
              "name": [
                "Departamento de Oceanograf\u00eda, Universidad de Concepci\u00f3n, Casilla 160-C, 4070386, Concepci\u00f3n, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ulloa", 
            "givenName": "Osvaldo", 
            "id": "sg:person.01132623201.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132623201.48"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Civil and Environmental Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA", 
              "id": "http://www.grid.ac/institutes/grid.213917.f", 
              "name": [
                "School of Civil and Environmental Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Konstantinidis", 
            "givenName": "Konstantinos T.", 
            "id": "sg:person.0665165336.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665165336.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biology and Nordic Center for Earth Evolution (NordCEE), University of Southern Denmark, Odense, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.10825.3e", 
              "name": [
                "Department of Biology and Nordic Center for Earth Evolution (NordCEE), University of Southern Denmark, Odense, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Thamdrup", 
            "givenName": "Bo", 
            "id": "sg:person.0702146201.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702146201.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA", 
              "id": "http://www.grid.ac/institutes/grid.213917.f", 
              "name": [
                "School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stewart", 
            "givenName": "Frank J.", 
            "id": "sg:person.01342110413.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342110413.80"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature19068", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048465291", 
              "https://doi.org/10.1038/nature19068"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00374-005-0004-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051596811", 
              "https://doi.org/10.1007/s00374-005-0004-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12352", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019299949", 
              "https://doi.org/10.1038/nature12352"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2015.44", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048770248", 
              "https://doi.org/10.1038/ismej.2015.44"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2011.178", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002460141", 
              "https://doi.org/10.1038/ismej.2011.178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001273287", 
              "https://doi.org/10.1038/nature10453"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ngeo1739", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018356622", 
              "https://doi.org/10.1038/ngeo1739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-017-0929-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032055421", 
              "https://doi.org/10.1007/s00248-017-0929-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08276", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008437958", 
              "https://doi.org/10.1038/nature08276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2015.262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000219962", 
              "https://doi.org/10.1038/ismej.2015.262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14856", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013910284", 
              "https://doi.org/10.1038/nature14856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2014.067", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013309795", 
              "https://doi.org/10.1038/nprot.2014.067"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2013.243", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015343058", 
              "https://doi.org/10.1038/ismej.2013.243"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature01947", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011279761", 
              "https://doi.org/10.1038/nature01947"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-07-10", 
        "datePublishedReg": "2018-07-10", 
        "description": "Anaerobic ammonium oxidation (anammox) contributes substantially to ocean nitrogen loss, particularly in anoxic marine zones (AMZs). Ammonium is scarce in AMZs, raising the hypothesis that organic nitrogen compounds may be ammonium sources for anammox. Biochemical measurements suggest that the organic compounds urea and cyanate can support anammox in AMZs. However, it is unclear if anammox bacteria degrade these compounds to ammonium themselves, or rely on other organisms for this process. Genes for urea degradation have not been found in anammox bacteria, and genomic evidence for cyanate use for anammox is limited to a cyanase gene recovered from the sediment bacterium Candidatus Scalindua profunda. Here, analysis of Ca. Scalindua single amplified genomes from the Eastern Tropical North Pacific AMZ revealed genes for urea degradation and transport, as well as for cyanate degradation. Urease and cyanase genes were transcribed, along with anammox genes, in the AMZ core where anammox rates peaked. Homologs of these genes were also detected in meta-omic datasets from major AMZs in the Eastern Tropical South Pacific and Arabian Sea. These results suggest that anammox bacteria from different ocean regions can directly access organic nitrogen substrates. Future studies should assess if and under what environmental conditions these substrates contribute to the ammonium budget for anammox.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41396-018-0223-9", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.5301165", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.4851875", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3001513", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3789926", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3582343", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1038436", 
            "issn": [
              "1751-7362", 
              "1751-7370"
            ], 
            "name": "The ISME Journal: Multidisciplinary Journal of Microbial Ecology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "11", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "keywords": [
          "anoxic marine zones", 
          "cyanase gene", 
          "meta-omics datasets", 
          "anammox bacteria", 
          "nitrogen substrates", 
          "organic nitrogen substrates", 
          "eastern tropical South Pacific", 
          "tropical South Pacific", 
          "different ocean regions", 
          "anammox genes", 
          "genomic evidence", 
          "cyanate degradation", 
          "transcriptomic evidence", 
          "urea degradation", 
          "genes", 
          "anaerobic ammonium oxidation", 
          "Arabian Sea", 
          "ocean regions", 
          "South Pacific", 
          "marine zone", 
          "environmental conditions", 
          "anammox rates", 
          "bacteria", 
          "single cells", 
          "organic nitrogen compounds", 
          "ammonium source", 
          "ammonium oxidation", 
          "anammox", 
          "analysis of Ca", 
          "nitrogen loss", 
          "Scalindua", 
          "genome", 
          "homolog", 
          "degradation", 
          "organisms", 
          "nitrogen compounds", 
          "substrate", 
          "organic compounds", 
          "Pacific", 
          "Sea", 
          "zone", 
          "profunda", 
          "budget", 
          "future studies", 
          "cells", 
          "urease", 
          "core", 
          "Ca", 
          "transport", 
          "ammonium", 
          "compounds", 
          "evidence", 
          "biochemical measurements", 
          "region", 
          "source", 
          "hypothesis", 
          "datasets", 
          "loss", 
          "conditions", 
          "measurements", 
          "analysis", 
          "oxidation", 
          "process", 
          "study", 
          "rate", 
          "results", 
          "use", 
          "cyanate use", 
          "sediment bacterium Candidatus Scalindua profunda", 
          "bacterium Candidatus Scalindua profunda", 
          "Candidatus Scalindua profunda", 
          "Scalindua profunda", 
          "Eastern Tropical North Pacific AMZ", 
          "Tropical North Pacific AMZ", 
          "North Pacific AMZ", 
          "Pacific AMZ", 
          "AMZ core", 
          "major AMZs", 
          "ammonium budget", 
          "alternative nitrogen substrates"
        ], 
        "name": "Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria", 
        "pagination": "2706-2722", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1105465332"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41396-018-0223-9"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "29991764"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41396-018-0223-9", 
          "https://app.dimensions.ai/details/publication/pub.1105465332"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-12-01T19:43", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_787.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41396-018-0223-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41396-018-0223-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41396-018-0223-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41396-018-0223-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41396-018-0223-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    376 TRIPLES      22 PREDICATES      133 URIs      110 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41396-018-0223-9 schema:about N04eff321b84448e39bb3263290dee5c5
    2 N1477e87fd0b344f1b732c0f0b00d85cb
    3 N18231dc445634284981df8412ba7be59
    4 N530e33da8da048b798c6070013108384
    5 N69cc2227a6494e62a6ba5a45deec0638
    6 N75632c1e06cd48c2aa871dd7e2fd74df
    7 N7ab84b082d6b4d3cb87b8f630575370b
    8 N86d4d9833ecb40b1a0cba20a3b70162e
    9 N954e139c00c54e09ab87683cc64d9506
    10 Na5b0751311b54a0ab9f9d01c81309d69
    11 Nc4de43238ee748f6b6de2d47f322618b
    12 Nf84d15bae0e04f2fa175d86404d841c0
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 anzsrc-for:0605
    16 schema:author Ncd29a6319f40408496e6e996c7f5852f
    17 schema:citation sg:pub.10.1007/s00248-017-0929-7
    18 sg:pub.10.1007/s00374-005-0004-2
    19 sg:pub.10.1038/ismej.2011.178
    20 sg:pub.10.1038/ismej.2013.243
    21 sg:pub.10.1038/ismej.2015.262
    22 sg:pub.10.1038/ismej.2015.44
    23 sg:pub.10.1038/nature01947
    24 sg:pub.10.1038/nature08276
    25 sg:pub.10.1038/nature10453
    26 sg:pub.10.1038/nature12352
    27 sg:pub.10.1038/nature14856
    28 sg:pub.10.1038/nature19068
    29 sg:pub.10.1038/ngeo1739
    30 sg:pub.10.1038/nprot.2014.067
    31 schema:datePublished 2018-07-10
    32 schema:datePublishedReg 2018-07-10
    33 schema:description Anaerobic ammonium oxidation (anammox) contributes substantially to ocean nitrogen loss, particularly in anoxic marine zones (AMZs). Ammonium is scarce in AMZs, raising the hypothesis that organic nitrogen compounds may be ammonium sources for anammox. Biochemical measurements suggest that the organic compounds urea and cyanate can support anammox in AMZs. However, it is unclear if anammox bacteria degrade these compounds to ammonium themselves, or rely on other organisms for this process. Genes for urea degradation have not been found in anammox bacteria, and genomic evidence for cyanate use for anammox is limited to a cyanase gene recovered from the sediment bacterium Candidatus Scalindua profunda. Here, analysis of Ca. Scalindua single amplified genomes from the Eastern Tropical North Pacific AMZ revealed genes for urea degradation and transport, as well as for cyanate degradation. Urease and cyanase genes were transcribed, along with anammox genes, in the AMZ core where anammox rates peaked. Homologs of these genes were also detected in meta-omic datasets from major AMZs in the Eastern Tropical South Pacific and Arabian Sea. These results suggest that anammox bacteria from different ocean regions can directly access organic nitrogen substrates. Future studies should assess if and under what environmental conditions these substrates contribute to the ammonium budget for anammox.
    34 schema:genre article
    35 schema:inLanguage en
    36 schema:isAccessibleForFree true
    37 schema:isPartOf Nb49db714a04546c7b9f15c4890a86cc9
    38 Ndfad5ba32ebd4a55b0b6152f0a99d976
    39 sg:journal.1038436
    40 schema:keywords AMZ core
    41 Arabian Sea
    42 Ca
    43 Candidatus Scalindua profunda
    44 Eastern Tropical North Pacific AMZ
    45 North Pacific AMZ
    46 Pacific
    47 Pacific AMZ
    48 Scalindua
    49 Scalindua profunda
    50 Sea
    51 South Pacific
    52 Tropical North Pacific AMZ
    53 alternative nitrogen substrates
    54 ammonium
    55 ammonium budget
    56 ammonium oxidation
    57 ammonium source
    58 anaerobic ammonium oxidation
    59 analysis
    60 analysis of Ca
    61 anammox
    62 anammox bacteria
    63 anammox genes
    64 anammox rates
    65 anoxic marine zones
    66 bacteria
    67 bacterium Candidatus Scalindua profunda
    68 biochemical measurements
    69 budget
    70 cells
    71 compounds
    72 conditions
    73 core
    74 cyanase gene
    75 cyanate degradation
    76 cyanate use
    77 datasets
    78 degradation
    79 different ocean regions
    80 eastern tropical South Pacific
    81 environmental conditions
    82 evidence
    83 future studies
    84 genes
    85 genome
    86 genomic evidence
    87 homolog
    88 hypothesis
    89 loss
    90 major AMZs
    91 marine zone
    92 measurements
    93 meta-omics datasets
    94 nitrogen compounds
    95 nitrogen loss
    96 nitrogen substrates
    97 ocean regions
    98 organic compounds
    99 organic nitrogen compounds
    100 organic nitrogen substrates
    101 organisms
    102 oxidation
    103 process
    104 profunda
    105 rate
    106 region
    107 results
    108 sediment bacterium Candidatus Scalindua profunda
    109 single cells
    110 source
    111 study
    112 substrate
    113 transcriptomic evidence
    114 transport
    115 tropical South Pacific
    116 urea degradation
    117 urease
    118 use
    119 zone
    120 schema:name Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria
    121 schema:pagination 2706-2722
    122 schema:productId N28cb8f2731814537b1744804a0f5e6db
    123 N8b86518bf0eb4e5591ce4a30bc56abb9
    124 N9afd725c860d4c66886cbca8264d5afd
    125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105465332
    126 https://doi.org/10.1038/s41396-018-0223-9
    127 schema:sdDatePublished 2021-12-01T19:43
    128 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    129 schema:sdPublisher Nfb4dc52f67a94bd7a0b9a198c777aabc
    130 schema:url https://doi.org/10.1038/s41396-018-0223-9
    131 sgo:license sg:explorer/license/
    132 sgo:sdDataset articles
    133 rdf:type schema:ScholarlyArticle
    134 N04eff321b84448e39bb3263290dee5c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Anaerobiosis
    136 rdf:type schema:DefinedTerm
    137 N0bb2ac32743148718c3138ce506ded26 rdf:first sg:person.01366555074.19
    138 rdf:rest Nae13490d195d4adc924b490d6c6db550
    139 N1477e87fd0b344f1b732c0f0b00d85cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Nitrogen
    141 rdf:type schema:DefinedTerm
    142 N18231dc445634284981df8412ba7be59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Oceans and Seas
    144 rdf:type schema:DefinedTerm
    145 N28cb8f2731814537b1744804a0f5e6db schema:name pubmed_id
    146 schema:value 29991764
    147 rdf:type schema:PropertyValue
    148 N32767dad18184dafa4883a63d6e7fdb3 rdf:first sg:person.0647045421.55
    149 rdf:rest Nc25ccdb3eb3e4252aef4fd483859d3ba
    150 N4a2fb0d559ff4b7f8a9d996e02e16233 rdf:first sg:person.012242753633.49
    151 rdf:rest N4c45b8cddcfe47ab8e64b28ec15ac5b6
    152 N4c45b8cddcfe47ab8e64b28ec15ac5b6 rdf:first sg:person.0736172076.44
    153 rdf:rest N60fc518bdec44a88af78880c23adf05c
    154 N4cade1c004d344a58c939116453f43e8 schema:affiliation grid-institutes:grid.25879.31
    155 schema:familyName Blackwood
    156 schema:givenName Nigel
    157 rdf:type schema:Person
    158 N530e33da8da048b798c6070013108384 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Urease
    160 rdf:type schema:DefinedTerm
    161 N5b86aebd2681407a80f87b97e63732a5 rdf:first sg:person.0665165336.02
    162 rdf:rest Nbf2d671acd4f4d48bdf326cf0d4a71da
    163 N60fc518bdec44a88af78880c23adf05c rdf:first sg:person.0627543264.08
    164 rdf:rest N9b60a0afd2794c6a99c04a8f88d1747e
    165 N69cc2227a6494e62a6ba5a45deec0638 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Oxidation-Reduction
    167 rdf:type schema:DefinedTerm
    168 N75632c1e06cd48c2aa871dd7e2fd74df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Carbon-Nitrogen Lyases
    170 rdf:type schema:DefinedTerm
    171 N7ab84b082d6b4d3cb87b8f630575370b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Ammonium Compounds
    173 rdf:type schema:DefinedTerm
    174 N86d4d9833ecb40b1a0cba20a3b70162e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Gene Expression Profiling
    176 rdf:type schema:DefinedTerm
    177 N8b86518bf0eb4e5591ce4a30bc56abb9 schema:name dimensions_id
    178 schema:value pub.1105465332
    179 rdf:type schema:PropertyValue
    180 N8f488a134c684829a204b72b2df65531 rdf:first sg:person.01111772272.70
    181 rdf:rest Nb5258d956a754eac9c5923c01c77a7b7
    182 N954e139c00c54e09ab87683cc64d9506 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Single-Cell Analysis
    184 rdf:type schema:DefinedTerm
    185 N9afd725c860d4c66886cbca8264d5afd schema:name doi
    186 schema:value 10.1038/s41396-018-0223-9
    187 rdf:type schema:PropertyValue
    188 N9b60a0afd2794c6a99c04a8f88d1747e rdf:first sg:person.01046703646.00
    189 rdf:rest N0bb2ac32743148718c3138ce506ded26
    190 Na177cad7145045a5b25ca1c3eb5e3100 rdf:first sg:person.01342110413.80
    191 rdf:rest rdf:nil
    192 Na5b0751311b54a0ab9f9d01c81309d69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Bacteria
    194 rdf:type schema:DefinedTerm
    195 Nae13490d195d4adc924b490d6c6db550 rdf:first sg:person.01132623201.48
    196 rdf:rest N5b86aebd2681407a80f87b97e63732a5
    197 Nb49db714a04546c7b9f15c4890a86cc9 schema:volumeNumber 12
    198 rdf:type schema:PublicationVolume
    199 Nb5258d956a754eac9c5923c01c77a7b7 rdf:first sg:person.0751537347.54
    200 rdf:rest N32767dad18184dafa4883a63d6e7fdb3
    201 Nbf2d671acd4f4d48bdf326cf0d4a71da rdf:first sg:person.0702146201.01
    202 rdf:rest Na177cad7145045a5b25ca1c3eb5e3100
    203 Nc25ccdb3eb3e4252aef4fd483859d3ba rdf:first N4cade1c004d344a58c939116453f43e8
    204 rdf:rest N4a2fb0d559ff4b7f8a9d996e02e16233
    205 Nc4de43238ee748f6b6de2d47f322618b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Seawater
    207 rdf:type schema:DefinedTerm
    208 Ncd29a6319f40408496e6e996c7f5852f rdf:first sg:person.0715160621.04
    209 rdf:rest N8f488a134c684829a204b72b2df65531
    210 Ndfad5ba32ebd4a55b0b6152f0a99d976 schema:issueNumber 11
    211 rdf:type schema:PublicationIssue
    212 Nf84d15bae0e04f2fa175d86404d841c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    213 schema:name Genomics
    214 rdf:type schema:DefinedTerm
    215 Nfb4dc52f67a94bd7a0b9a198c777aabc schema:name Springer Nature - SN SciGraph project
    216 rdf:type schema:Organization
    217 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    218 schema:name Biological Sciences
    219 rdf:type schema:DefinedTerm
    220 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    221 schema:name Genetics
    222 rdf:type schema:DefinedTerm
    223 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    224 schema:name Microbiology
    225 rdf:type schema:DefinedTerm
    226 sg:grant.3001513 http://pending.schema.org/fundedItem sg:pub.10.1038/s41396-018-0223-9
    227 rdf:type schema:MonetaryGrant
    228 sg:grant.3582343 http://pending.schema.org/fundedItem sg:pub.10.1038/s41396-018-0223-9
    229 rdf:type schema:MonetaryGrant
    230 sg:grant.3789926 http://pending.schema.org/fundedItem sg:pub.10.1038/s41396-018-0223-9
    231 rdf:type schema:MonetaryGrant
    232 sg:grant.4851875 http://pending.schema.org/fundedItem sg:pub.10.1038/s41396-018-0223-9
    233 rdf:type schema:MonetaryGrant
    234 sg:grant.5301165 http://pending.schema.org/fundedItem sg:pub.10.1038/s41396-018-0223-9
    235 rdf:type schema:MonetaryGrant
    236 sg:journal.1038436 schema:issn 1751-7362
    237 1751-7370
    238 schema:name The ISME Journal: Multidisciplinary Journal of Microbial Ecology
    239 schema:publisher Springer Nature
    240 rdf:type schema:Periodical
    241 sg:person.01046703646.00 schema:affiliation grid-institutes:grid.451309.a
    242 schema:familyName Malmstrom
    243 schema:givenName Rex R.
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046703646.00
    245 rdf:type schema:Person
    246 sg:person.01111772272.70 schema:affiliation grid-institutes:grid.213917.f
    247 schema:familyName Bertagnolli
    248 schema:givenName Anthony D.
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111772272.70
    250 rdf:type schema:Person
    251 sg:person.01132623201.48 schema:affiliation grid-institutes:grid.5380.e
    252 schema:familyName Ulloa
    253 schema:givenName Osvaldo
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132623201.48
    255 rdf:type schema:Person
    256 sg:person.012242753633.49 schema:affiliation grid-institutes:grid.5380.e
    257 schema:familyName Aldunate
    258 schema:givenName Montserrat
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012242753633.49
    260 rdf:type schema:Person
    261 sg:person.01342110413.80 schema:affiliation grid-institutes:grid.213917.f
    262 schema:familyName Stewart
    263 schema:givenName Frank J.
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342110413.80
    265 rdf:type schema:Person
    266 sg:person.01366555074.19 schema:affiliation grid-institutes:grid.451309.a
    267 schema:familyName Woyke
    268 schema:givenName Tanja
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366555074.19
    270 rdf:type schema:Person
    271 sg:person.0627543264.08 schema:affiliation grid-institutes:grid.266686.a
    272 schema:familyName Altabet
    273 schema:givenName Mark A.
    274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627543264.08
    275 rdf:type schema:Person
    276 sg:person.0647045421.55 schema:affiliation grid-institutes:grid.213917.f
    277 schema:familyName Padilla
    278 schema:givenName Cory C.
    279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647045421.55
    280 rdf:type schema:Person
    281 sg:person.0665165336.02 schema:affiliation grid-institutes:grid.213917.f
    282 schema:familyName Konstantinidis
    283 schema:givenName Konstantinos T.
    284 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665165336.02
    285 rdf:type schema:Person
    286 sg:person.0702146201.01 schema:affiliation grid-institutes:grid.10825.3e
    287 schema:familyName Thamdrup
    288 schema:givenName Bo
    289 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702146201.01
    290 rdf:type schema:Person
    291 sg:person.0715160621.04 schema:affiliation grid-institutes:grid.451178.e
    292 schema:familyName Ganesh
    293 schema:givenName Sangita
    294 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715160621.04
    295 rdf:type schema:Person
    296 sg:person.0736172076.44 schema:affiliation grid-institutes:grid.266686.a
    297 schema:familyName Bourbonnais
    298 schema:givenName Annie
    299 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736172076.44
    300 rdf:type schema:Person
    301 sg:person.0751537347.54 schema:affiliation grid-institutes:grid.419529.2
    302 schema:familyName Bristow
    303 schema:givenName Laura A.
    304 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751537347.54
    305 rdf:type schema:Person
    306 sg:pub.10.1007/s00248-017-0929-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032055421
    307 https://doi.org/10.1007/s00248-017-0929-7
    308 rdf:type schema:CreativeWork
    309 sg:pub.10.1007/s00374-005-0004-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051596811
    310 https://doi.org/10.1007/s00374-005-0004-2
    311 rdf:type schema:CreativeWork
    312 sg:pub.10.1038/ismej.2011.178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002460141
    313 https://doi.org/10.1038/ismej.2011.178
    314 rdf:type schema:CreativeWork
    315 sg:pub.10.1038/ismej.2013.243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015343058
    316 https://doi.org/10.1038/ismej.2013.243
    317 rdf:type schema:CreativeWork
    318 sg:pub.10.1038/ismej.2015.262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000219962
    319 https://doi.org/10.1038/ismej.2015.262
    320 rdf:type schema:CreativeWork
    321 sg:pub.10.1038/ismej.2015.44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048770248
    322 https://doi.org/10.1038/ismej.2015.44
    323 rdf:type schema:CreativeWork
    324 sg:pub.10.1038/nature01947 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011279761
    325 https://doi.org/10.1038/nature01947
    326 rdf:type schema:CreativeWork
    327 sg:pub.10.1038/nature08276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008437958
    328 https://doi.org/10.1038/nature08276
    329 rdf:type schema:CreativeWork
    330 sg:pub.10.1038/nature10453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001273287
    331 https://doi.org/10.1038/nature10453
    332 rdf:type schema:CreativeWork
    333 sg:pub.10.1038/nature12352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019299949
    334 https://doi.org/10.1038/nature12352
    335 rdf:type schema:CreativeWork
    336 sg:pub.10.1038/nature14856 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013910284
    337 https://doi.org/10.1038/nature14856
    338 rdf:type schema:CreativeWork
    339 sg:pub.10.1038/nature19068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048465291
    340 https://doi.org/10.1038/nature19068
    341 rdf:type schema:CreativeWork
    342 sg:pub.10.1038/ngeo1739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018356622
    343 https://doi.org/10.1038/ngeo1739
    344 rdf:type schema:CreativeWork
    345 sg:pub.10.1038/nprot.2014.067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013309795
    346 https://doi.org/10.1038/nprot.2014.067
    347 rdf:type schema:CreativeWork
    348 grid-institutes:grid.10825.3e schema:alternateName Department of Biology and Nordic Center for Earth Evolution (NordCEE), University of Southern Denmark, Odense, Denmark
    349 schema:name Department of Biology and Nordic Center for Earth Evolution (NordCEE), University of Southern Denmark, Odense, Denmark
    350 rdf:type schema:Organization
    351 grid-institutes:grid.213917.f schema:alternateName School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA
    352 School of Civil and Environmental Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA
    353 schema:name School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA
    354 School of Civil and Environmental Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA
    355 rdf:type schema:Organization
    356 grid-institutes:grid.25879.31 schema:alternateName Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA
    357 schema:name Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA
    358 rdf:type schema:Organization
    359 grid-institutes:grid.266686.a schema:alternateName School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA
    360 schema:name Marine Chemistry & Geochemistry, Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543, Woods Hole, MA, USA
    361 School for Marine Science and Technology, University of Massachusetts Dartmouth, 706 Rodney French Blvd, 02744, New Bedford, MA, USA
    362 rdf:type schema:Organization
    363 grid-institutes:grid.419529.2 schema:alternateName Biogeochemistry Group, Max Planck Institute for Marine Microbiology, Bremen, Germany
    364 schema:name Biogeochemistry Group, Max Planck Institute for Marine Microbiology, Bremen, Germany
    365 rdf:type schema:Organization
    366 grid-institutes:grid.451178.e schema:alternateName Radiant Genomics, 94608, Emeryville, CA, USA
    367 schema:name Radiant Genomics, 94608, Emeryville, CA, USA
    368 School of Biological Sciences, Georgia Institute of Technology, 30332, Atlanta, GA, USA
    369 rdf:type schema:Organization
    370 grid-institutes:grid.451309.a schema:alternateName Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA
    371 schema:name Department of Energy Joint Genome Institute, 2800 Mitchell Drive, 94598, Walnut Creek, CA, USA
    372 rdf:type schema:Organization
    373 grid-institutes:grid.5380.e schema:alternateName Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, 4070386, Concepción, Chile
    374 schema:name Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, 4070386, Concepción, Chile
    375 Graduate Program in Oceanography, Department of Oceanography, Faculty of Natural Sciences and Oceanography, University of Concepción, Casilla 160-C, Concepción, Chile
    376 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...