Hydrocarbon degradation and response of seafloor sediment bacterial community in the northern Gulf of Mexico to light Louisiana sweet crude ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-06-27

AUTHORS

Hernando P. Bacosa, Deana L. Erdner, Brad E. Rosenheim, Prateek Shetty, Kiley W. Seitz, Brett J. Baker, Zhanfei Liu

ABSTRACT

The Deepwater Horizon (DWH) blowout resulted in the deposition to the seafloor of up to 4.9% of 200 million gallons of oil released into the Gulf of Mexico. The petroleum hydrocarbon concentrations near the wellhead were high immediately after the spill, but returned to background levels a few years after the spill. Microbial communities in the seafloor are thought to be responsible for the degradation of hydrocarbons, however, our knowledge is primarily based upon gene diversity surveys and hydrocarbon concentration in field sediment samples. Here, we investigated the oil degradation potential and changes in bacterial community by amending seafloor sediment collected near the DWH site with crude oil and both oil and Corexit dispersant. Polycyclic aromatic hydrocarbons were rapidly degraded during the first 30 days of incubation, while alkanes were degraded more slowly. With the degradation of hydrocarbons, the relative abundances of Colwelliaceae, Alteromonadaceae, Methylococales, Alcanivorax, Bacteriovorax, and Phaeobacter increased remarkably. However, the abundances of oil-degrading bacteria changed with oil chemistry. Colwelliaceae decreased with increasing oil degradation, whereas Alcanivorax and Methylococcales increased considerably. We assembled seven genomes from the metagenome, including ones belonging to Colwellia, Alteromonadaceae, Rhodobacteraceae, the newly reported genus Woeseia, and candidate phylum NC10, all of which possess a repertoire of genes for hydrocarbon degradation. Moreover, genes related to hydrocarbon degradation were highly enriched in the oiled treatment, suggesting that the hydrocarbons were biodegraded, and that the indigenous microflora have a remarkable potential for the natural attenuation of spilled oil in the deep-sea surface sediment. More... »

PAGES

2532-2543

References to SciGraph publications

  • 2010-04-11. QIIME allows analysis of high-throughput community sequencing data in NATURE METHODS
  • 2009-08-21. Community-wide analysis of microbial genome sequence signatures in GENOME BIOLOGY
  • 2011-08-04. Microbial gene functions enriched in the Deepwater Horizon deep-sea oil plume in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-09-01. A novel denitrifying methanotroph of the NC10 phylum and its microcolony in SCIENTIFIC REPORTS
  • 2017-08-04. Metabolic and spatio-taxonomic response of uncultivated seafloor bacteria following the Deepwater Horizon oil spill in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2011-06-11. Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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  • 2016-05-09. Reconstructing metabolic pathways of hydrocarbon-degrading bacteria from the Deepwater Horizon oil spill in NATURE MICROBIOLOGY
  • 2010-09-15. Preferential utilization of petroleum oil hydrocarbon components by microbial consortia reflects degradation pattern in aliphatic–aromatic hydrocarbon binary mixtures in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013-06-20. Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2014-01-23. Metagenomics reveals sediment microbial community response to Deepwater Horizon oil spill in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-01-29. Genomic reconstruction of a novel, deeply branched sediment archaeal phylum with pathways for acetogenesis and sulfur reduction in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41396-018-0190-1

    DOI

    http://dx.doi.org/10.1038/s41396-018-0190-1

    DIMENSIONS

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    PUBMED

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    96 one
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    98 polycyclic aromatic hydrocarbons
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    100 relative abundance
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    102 repertoire
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    104 response
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    106 seafloor
    107 seafloor sediments
    108 sediment bacterial communities
    109 sediment samples
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