A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-05

AUTHORS

Vennila Ponnusamy, Olga Kapellou, Ellen Yip, Jane Evanson, Liang-Fong Wong, Adina Michael-Titus, Ping K. Yip, Divyen K. Shah

ABSTRACT

BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes. METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6-mm-diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma, and urine. Three miRNAs (RNU6B, let7b, and miR-21) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma, and urine. Secondarily, candidate miRNAs let7b, miR-21, miR-29b, miR-124, and miR-155 in DBS were evaluated as potential biomarkers for HIE. RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs' levels in DBS and EDTA-blood at -80 °C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE. CONCLUSION: DBS may be useful for studying the potential of miRNAs as biomarkers for brain injury. More... »

PAGES

799-805

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/pr.2015.276

DOI

http://dx.doi.org/10.1038/pr.2015.276

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026874153

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26720606


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Cardiorespiratory Medicine and Haematology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Asphyxia Neonatorum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dried Blood Spot Testing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hypoxia-Ischemia, Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Infant, Newborn", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "MicroRNAs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neonatal Screening", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ashford and St Peter's Hospitals NHS Foundation Trust", 
          "id": "https://www.grid.ac/institutes/grid.440168.f", 
          "name": [
            "Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK", 
            "Neonatal Unit, The Royal London Hospital, Barts Health NHS Trust, London, UK", 
            "Neonatal Intensive Care Unit, Ashford and St. Peter\u2019s Hospitals NHS Foundation Trust, Chertsey, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ponnusamy", 
        "givenName": "Vennila", 
        "id": "sg:person.0665752707.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665752707.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Health Service", 
          "id": "https://www.grid.ac/institutes/grid.451052.7", 
          "name": [
            "Neonatal Intensive Care Unit, Homerton University Hospitals NHS Foundation Trust, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kapellou", 
        "givenName": "Olga", 
        "id": "sg:person.01041705022.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041705022.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queen Mary University of London", 
          "id": "https://www.grid.ac/institutes/grid.4868.2", 
          "name": [
            "Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yip", 
        "givenName": "Ellen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Royal London Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416041.6", 
          "name": [
            "Imaging Services, The Royal London Hospital, Barts Health NHS Trust, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Evanson", 
        "givenName": "Jane", 
        "id": "sg:person.01065052145.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065052145.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Bristol", 
          "id": "https://www.grid.ac/institutes/grid.5337.2", 
          "name": [
            "School of Clinical Sciences, University of Bristol, Bristol, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wong", 
        "givenName": "Liang-Fong", 
        "id": "sg:person.0766413460.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766413460.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queen Mary University of London", 
          "id": "https://www.grid.ac/institutes/grid.4868.2", 
          "name": [
            "Center of Neuroscience and Trauma, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Michael-Titus", 
        "givenName": "Adina", 
        "id": "sg:person.01137704550.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137704550.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queen Mary University of London", 
          "id": "https://www.grid.ac/institutes/grid.4868.2", 
          "name": [
            "The last two authors contributed equally to this work.", 
            "Center of Neuroscience and Trauma, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yip", 
        "givenName": "Ping K.", 
        "id": "sg:person.01173502202.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173502202.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Royal London Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416041.6", 
          "name": [
            "The last two authors contributed equally to this work.", 
            "Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK", 
            "Neonatal Unit, The Royal London Hospital, Barts Health NHS Trust, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shah", 
        "givenName": "Divyen K.", 
        "id": "sg:person.01173642071.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173642071.88"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/gb-2004-5-3-r13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000703776", 
          "https://doi.org/10.1186/gb-2004-5-3-r13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.2006.118364", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000847559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0103948", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007905784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14767050903181468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008624257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2377-13-178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008966346", 
          "https://doi.org/10.1186/1471-2377-13-178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4172/2168-9784.1000125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010050347"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fncel.2015.00193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011518142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2013-14-7-r78", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012389768", 
          "https://doi.org/10.1186/gb-2013-14-7-r78"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/cc13753", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014740245", 
          "https://doi.org/10.1186/cc13753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2010.07818.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015718530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2431-8-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017580074", 
          "https://doi.org/10.1186/1471-2431-8-17"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.siny.2012.07.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019641599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023682432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2010.08.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025619687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2014.07.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028126632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1373/clinchem.2010.157198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028432883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.earlhumdev.2011.01.041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028745674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jpeds.2013.10.067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030367138"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jpeds.2013.10.067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030367138"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2012.09.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031731019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.neuro.29.051605.112839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031973323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1067/mcp.2001.113989", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032912526", 
          "https://doi.org/10.1067/mcp.2001.113989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1975411", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034416119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2007.07.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034464351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2567.2011.03514.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035227232"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1474-4422(09)70295-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036557550"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0024145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039286459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pediatrneurol.2008.09.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039719210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/nen.0000000000000091", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040586240"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg2936", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046015375", 
          "https://doi.org/10.1038/nrg2936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0077963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047431319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/01.pdr.0000144819.45689.bb", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047521261", 
          "https://doi.org/10.1203/01.pdr.0000144819.45689.bb"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/01.pdr.0000144819.45689.bb", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047521261", 
          "https://doi.org/10.1203/01.pdr.0000144819.45689.bb"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn.3113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053133452", 
          "https://doi.org/10.1038/nn.3113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2014.09.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053242147"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-05", 
    "datePublishedReg": "2016-05-01", 
    "description": "BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.\nMETHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6-mm-diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma, and urine. Three miRNAs (RNU6B, let7b, and miR-21) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma, and urine. Secondarily, candidate miRNAs let7b, miR-21, miR-29b, miR-124, and miR-155 in DBS were evaluated as potential biomarkers for HIE.\nRESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs' levels in DBS and EDTA-blood at -80 \u00b0C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.\nCONCLUSION: DBS may be useful for studying the potential of miRNAs as biomarkers for brain injury.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/pr.2015.276", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2778662", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1007352", 
        "issn": [
          "0031-3998", 
          "1530-0447"
        ], 
        "name": "Pediatric Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "79"
      }
    ], 
    "name": "A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method", 
    "pagination": "799-805", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8005e69ab9464ebb79f83e7423940cb891e78a1a53c2128f20051b139fb19f2c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26720606"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0100714"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/pr.2015.276"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026874153"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/pr.2015.276", 
      "https://app.dimensions.ai/details/publication/pub.1026874153"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/pr/journal/v79/n5/full/pr2015276a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/pr.2015.276'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/pr.2015.276'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/pr.2015.276'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/pr.2015.276'


 

This table displays all metadata directly associated to this object as RDF triples.

298 TRIPLES      21 PREDICATES      75 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/pr.2015.276 schema:about N0825eff44f5245d1b8fd523e22d83cdd
2 N12b40bcbc0334e8e89aff80464404ffa
3 N1bfbb91961144b2faaf6aa17ca4da721
4 N207c12958f2a4e199a1d3dffdc44dd93
5 N26999ccfa6e84475b9fc822257dd89c1
6 N3c83ba4e09e643229809fb6fc395d717
7 N58a2865ac54e44f3bafcc897b6d972eb
8 N67a22f81540e4fe4b50aaed78b689699
9 N7e6e0085f1bd46208edc8a7a2b6d7b80
10 N820d0f7e767b4807b265230ca826a0dc
11 N8d166cef908c4318ba537c7c0a6a2a37
12 Nc02ccbde5f4f49649b482b466b2cd095
13 Nc2b00e3611014974a561ec82e6772c19
14 anzsrc-for:11
15 anzsrc-for:1102
16 schema:author N4607af5aa8454bf28e516ae2da91b2da
17 schema:citation sg:pub.10.1038/nn.3113
18 sg:pub.10.1038/nrg2936
19 sg:pub.10.1067/mcp.2001.113989
20 sg:pub.10.1186/1471-2377-13-178
21 sg:pub.10.1186/1471-2431-8-17
22 sg:pub.10.1186/cc13753
23 sg:pub.10.1186/gb-2004-5-3-r13
24 sg:pub.10.1186/gb-2013-14-7-r78
25 sg:pub.10.1203/01.pdr.0000144819.45689.bb
26 https://doi.org/10.1016/j.ab.2010.08.004
27 https://doi.org/10.1016/j.ab.2012.09.007
28 https://doi.org/10.1016/j.bbadis.2014.07.021
29 https://doi.org/10.1016/j.cell.2009.01.002
30 https://doi.org/10.1016/j.earlhumdev.2011.01.041
31 https://doi.org/10.1016/j.jpeds.2013.10.067
32 https://doi.org/10.1016/j.molcel.2007.07.015
33 https://doi.org/10.1016/j.neuint.2014.09.009
34 https://doi.org/10.1016/j.pediatrneurol.2008.09.026
35 https://doi.org/10.1016/j.siny.2012.07.008
36 https://doi.org/10.1016/s1474-4422(09)70295-9
37 https://doi.org/10.1080/14767050903181468
38 https://doi.org/10.1097/nen.0000000000000091
39 https://doi.org/10.1101/gad.1975411
40 https://doi.org/10.1111/j.1365-2567.2011.03514.x
41 https://doi.org/10.1111/j.1742-4658.2010.07818.x
42 https://doi.org/10.1113/jphysiol.2006.118364
43 https://doi.org/10.1146/annurev.neuro.29.051605.112839
44 https://doi.org/10.1371/journal.pone.0024145
45 https://doi.org/10.1371/journal.pone.0077963
46 https://doi.org/10.1371/journal.pone.0103948
47 https://doi.org/10.1373/clinchem.2010.157198
48 https://doi.org/10.3389/fncel.2015.00193
49 https://doi.org/10.4172/2168-9784.1000125
50 schema:datePublished 2016-05
51 schema:datePublishedReg 2016-05-01
52 schema:description BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes. METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6-mm-diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma, and urine. Three miRNAs (RNU6B, let7b, and miR-21) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma, and urine. Secondarily, candidate miRNAs let7b, miR-21, miR-29b, miR-124, and miR-155 in DBS were evaluated as potential biomarkers for HIE. RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs' levels in DBS and EDTA-blood at -80 °C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE. CONCLUSION: DBS may be useful for studying the potential of miRNAs as biomarkers for brain injury.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf Na17e6e2b61f044dda756b2bbe06d9cf2
57 Nbc4b015bd07f4398958421cf17c2a13a
58 sg:journal.1007352
59 schema:name A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method
60 schema:pagination 799-805
61 schema:productId N20828859dd144df6a78a8311df5648fa
62 N32a4716c638343e7bcc9087943ba8472
63 N3580c355797a45d181a9f3534163448d
64 Ndbbd7001326f407ea493457a61eb6a07
65 Nff5c507527dd4346b2c2a5a8c0577407
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026874153
67 https://doi.org/10.1038/pr.2015.276
68 schema:sdDatePublished 2019-04-10T13:55
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N181c360791bc4d569c06adba1c9c3767
71 schema:url http://www.nature.com/pr/journal/v79/n5/full/pr2015276a.html
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N0825eff44f5245d1b8fd523e22d83cdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Gene Expression Profiling
77 rdf:type schema:DefinedTerm
78 N12b40bcbc0334e8e89aff80464404ffa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Infant, Newborn
80 rdf:type schema:DefinedTerm
81 N181c360791bc4d569c06adba1c9c3767 schema:name Springer Nature - SN SciGraph project
82 rdf:type schema:Organization
83 N1bfbb91961144b2faaf6aa17ca4da721 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Asphyxia Neonatorum
85 rdf:type schema:DefinedTerm
86 N207c12958f2a4e199a1d3dffdc44dd93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Biomarkers
88 rdf:type schema:DefinedTerm
89 N20828859dd144df6a78a8311df5648fa schema:name dimensions_id
90 schema:value pub.1026874153
91 rdf:type schema:PropertyValue
92 N26999ccfa6e84475b9fc822257dd89c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Female
94 rdf:type schema:DefinedTerm
95 N32a4716c638343e7bcc9087943ba8472 schema:name readcube_id
96 schema:value 8005e69ab9464ebb79f83e7423940cb891e78a1a53c2128f20051b139fb19f2c
97 rdf:type schema:PropertyValue
98 N3580c355797a45d181a9f3534163448d schema:name nlm_unique_id
99 schema:value 0100714
100 rdf:type schema:PropertyValue
101 N37987a20c39249f2973f9b8f65d9003f rdf:first sg:person.01137704550.70
102 rdf:rest N3d5b1c1fb61c4b658e7b3ba4b16c91f6
103 N3c83ba4e09e643229809fb6fc395d717 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Lung
105 rdf:type schema:DefinedTerm
106 N3d5b1c1fb61c4b658e7b3ba4b16c91f6 rdf:first sg:person.01173502202.45
107 rdf:rest N8746cdb0413a49b786becc27bc7b717b
108 N4607af5aa8454bf28e516ae2da91b2da rdf:first sg:person.0665752707.86
109 rdf:rest N904261d5a3bc4154b3dcc8d7b80e61a8
110 N4d3923385ca84141a1b905397ec08b60 rdf:first sg:person.01065052145.15
111 rdf:rest Neac97f33229e47708018b26b5c2fa00f
112 N58a2865ac54e44f3bafcc897b6d972eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Humans
114 rdf:type schema:DefinedTerm
115 N62930b11a6074309a868c3f8a94983fd rdf:first N7cbf2bb9376646f5bbfc10c76d02224c
116 rdf:rest N4d3923385ca84141a1b905397ec08b60
117 N67a22f81540e4fe4b50aaed78b689699 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Neonatal Screening
119 rdf:type schema:DefinedTerm
120 N7cbf2bb9376646f5bbfc10c76d02224c schema:affiliation https://www.grid.ac/institutes/grid.4868.2
121 schema:familyName Yip
122 schema:givenName Ellen
123 rdf:type schema:Person
124 N7e6e0085f1bd46208edc8a7a2b6d7b80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Hydrogen-Ion Concentration
126 rdf:type schema:DefinedTerm
127 N820d0f7e767b4807b265230ca826a0dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Dried Blood Spot Testing
129 rdf:type schema:DefinedTerm
130 N8746cdb0413a49b786becc27bc7b717b rdf:first sg:person.01173642071.88
131 rdf:rest rdf:nil
132 N8d166cef908c4318ba537c7c0a6a2a37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name MicroRNAs
134 rdf:type schema:DefinedTerm
135 N904261d5a3bc4154b3dcc8d7b80e61a8 rdf:first sg:person.01041705022.25
136 rdf:rest N62930b11a6074309a868c3f8a94983fd
137 Na17e6e2b61f044dda756b2bbe06d9cf2 schema:volumeNumber 79
138 rdf:type schema:PublicationVolume
139 Nbc4b015bd07f4398958421cf17c2a13a schema:issueNumber 5
140 rdf:type schema:PublicationIssue
141 Nc02ccbde5f4f49649b482b466b2cd095 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Hypoxia-Ischemia, Brain
143 rdf:type schema:DefinedTerm
144 Nc2b00e3611014974a561ec82e6772c19 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Male
146 rdf:type schema:DefinedTerm
147 Ndbbd7001326f407ea493457a61eb6a07 schema:name doi
148 schema:value 10.1038/pr.2015.276
149 rdf:type schema:PropertyValue
150 Neac97f33229e47708018b26b5c2fa00f rdf:first sg:person.0766413460.50
151 rdf:rest N37987a20c39249f2973f9b8f65d9003f
152 Nff5c507527dd4346b2c2a5a8c0577407 schema:name pubmed_id
153 schema:value 26720606
154 rdf:type schema:PropertyValue
155 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
156 schema:name Medical and Health Sciences
157 rdf:type schema:DefinedTerm
158 anzsrc-for:1102 schema:inDefinedTermSet anzsrc-for:
159 schema:name Cardiorespiratory Medicine and Haematology
160 rdf:type schema:DefinedTerm
161 sg:grant.2778662 http://pending.schema.org/fundedItem sg:pub.10.1038/pr.2015.276
162 rdf:type schema:MonetaryGrant
163 sg:journal.1007352 schema:issn 0031-3998
164 1530-0447
165 schema:name Pediatric Research
166 rdf:type schema:Periodical
167 sg:person.01041705022.25 schema:affiliation https://www.grid.ac/institutes/grid.451052.7
168 schema:familyName Kapellou
169 schema:givenName Olga
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041705022.25
171 rdf:type schema:Person
172 sg:person.01065052145.15 schema:affiliation https://www.grid.ac/institutes/grid.416041.6
173 schema:familyName Evanson
174 schema:givenName Jane
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065052145.15
176 rdf:type schema:Person
177 sg:person.01137704550.70 schema:affiliation https://www.grid.ac/institutes/grid.4868.2
178 schema:familyName Michael-Titus
179 schema:givenName Adina
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137704550.70
181 rdf:type schema:Person
182 sg:person.01173502202.45 schema:affiliation https://www.grid.ac/institutes/grid.4868.2
183 schema:familyName Yip
184 schema:givenName Ping K.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173502202.45
186 rdf:type schema:Person
187 sg:person.01173642071.88 schema:affiliation https://www.grid.ac/institutes/grid.416041.6
188 schema:familyName Shah
189 schema:givenName Divyen K.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173642071.88
191 rdf:type schema:Person
192 sg:person.0665752707.86 schema:affiliation https://www.grid.ac/institutes/grid.440168.f
193 schema:familyName Ponnusamy
194 schema:givenName Vennila
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665752707.86
196 rdf:type schema:Person
197 sg:person.0766413460.50 schema:affiliation https://www.grid.ac/institutes/grid.5337.2
198 schema:familyName Wong
199 schema:givenName Liang-Fong
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766413460.50
201 rdf:type schema:Person
202 sg:pub.10.1038/nn.3113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053133452
203 https://doi.org/10.1038/nn.3113
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nrg2936 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046015375
206 https://doi.org/10.1038/nrg2936
207 rdf:type schema:CreativeWork
208 sg:pub.10.1067/mcp.2001.113989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032912526
209 https://doi.org/10.1067/mcp.2001.113989
210 rdf:type schema:CreativeWork
211 sg:pub.10.1186/1471-2377-13-178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008966346
212 https://doi.org/10.1186/1471-2377-13-178
213 rdf:type schema:CreativeWork
214 sg:pub.10.1186/1471-2431-8-17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017580074
215 https://doi.org/10.1186/1471-2431-8-17
216 rdf:type schema:CreativeWork
217 sg:pub.10.1186/cc13753 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014740245
218 https://doi.org/10.1186/cc13753
219 rdf:type schema:CreativeWork
220 sg:pub.10.1186/gb-2004-5-3-r13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000703776
221 https://doi.org/10.1186/gb-2004-5-3-r13
222 rdf:type schema:CreativeWork
223 sg:pub.10.1186/gb-2013-14-7-r78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012389768
224 https://doi.org/10.1186/gb-2013-14-7-r78
225 rdf:type schema:CreativeWork
226 sg:pub.10.1203/01.pdr.0000144819.45689.bb schema:sameAs https://app.dimensions.ai/details/publication/pub.1047521261
227 https://doi.org/10.1203/01.pdr.0000144819.45689.bb
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.ab.2010.08.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025619687
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/j.ab.2012.09.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031731019
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/j.bbadis.2014.07.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028126632
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/j.cell.2009.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023682432
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/j.earlhumdev.2011.01.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028745674
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/j.jpeds.2013.10.067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030367138
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.molcel.2007.07.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034464351
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.neuint.2014.09.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053242147
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.pediatrneurol.2008.09.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039719210
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.siny.2012.07.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019641599
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s1474-4422(09)70295-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036557550
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1080/14767050903181468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008624257
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1097/nen.0000000000000091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040586240
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1101/gad.1975411 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034416119
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1111/j.1365-2567.2011.03514.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035227232
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1111/j.1742-4658.2010.07818.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015718530
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1113/jphysiol.2006.118364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000847559
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1146/annurev.neuro.29.051605.112839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031973323
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1371/journal.pone.0024145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039286459
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1371/journal.pone.0077963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047431319
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1371/journal.pone.0103948 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007905784
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1373/clinchem.2010.157198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028432883
272 rdf:type schema:CreativeWork
273 https://doi.org/10.3389/fncel.2015.00193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011518142
274 rdf:type schema:CreativeWork
275 https://doi.org/10.4172/2168-9784.1000125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010050347
276 rdf:type schema:CreativeWork
277 https://www.grid.ac/institutes/grid.416041.6 schema:alternateName Royal London Hospital
278 schema:name Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK
279 Imaging Services, The Royal London Hospital, Barts Health NHS Trust, London, UK
280 Neonatal Unit, The Royal London Hospital, Barts Health NHS Trust, London, UK
281 The last two authors contributed equally to this work.
282 rdf:type schema:Organization
283 https://www.grid.ac/institutes/grid.440168.f schema:alternateName Ashford and St Peter's Hospitals NHS Foundation Trust
284 schema:name Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK
285 Neonatal Intensive Care Unit, Ashford and St. Peter’s Hospitals NHS Foundation Trust, Chertsey, UK
286 Neonatal Unit, The Royal London Hospital, Barts Health NHS Trust, London, UK
287 rdf:type schema:Organization
288 https://www.grid.ac/institutes/grid.451052.7 schema:alternateName National Health Service
289 schema:name Neonatal Intensive Care Unit, Homerton University Hospitals NHS Foundation Trust, London, UK
290 rdf:type schema:Organization
291 https://www.grid.ac/institutes/grid.4868.2 schema:alternateName Queen Mary University of London
292 schema:name Center of Neuroscience and Trauma, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK
293 Center of Paediatrics, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK
294 The last two authors contributed equally to this work.
295 rdf:type schema:Organization
296 https://www.grid.ac/institutes/grid.5337.2 schema:alternateName University of Bristol
297 schema:name School of Clinical Sciences, University of Bristol, Bristol, UK
298 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...