Ontology type: schema:ScholarlyArticle Open Access: True
2015-08
AUTHORSA A Bhat, J L Pope, J J Smith, R Ahmad, X Chen, M K Washington, R D Beauchamp, A B Singh, P Dhawan
ABSTRACTIn normal colon, claudin-7 is one of the highly expressed claudin proteins and its knockdown in mice results in altered epithelial cell homeostasis and neonatal death. Notably, dysregulation of the epithelial homeostasis potentiates oncogenic transformation and growth. However, the role of claudin-7 in the regulation of colon tumorigenesis remains poorly understood. Using a large colorectal cancer (CRC) patient database and mouse models of colon cancer, we found claudin-7 expression to be significantly downregulated in cancer samples. Most notably, forced claudin-7 expression in poorly differentiated and highly metastatic SW620 colon cancer cells induced epithelial characteristics and inhibited their growth in soft agar and tumor growth in vivo. By contrast, knockdown of claudin-7 in HT-29 or DLD-1 cells induced epithelial-to-mesenchymal transition (EMT), colony formation, xenograft-tumor growth in athymic mice and invasion. Importantly, a claudin-7 signature gene profile generated by overlapping the DEGs (differentially expressed genes in a high-throughput transcriptome analysis using claudin-7-manipulated cells) with human claudin-7 signature genes identified high-risk CRC patients. Furthermore, Rab25, a colon cancer suppressor and regulator of the polarized cell trafficking constituted one of the highly upregulated DEGs in claudin-7 overexpressing cells. Notably, silencing of Rab25 expression counteracted the effects of claudin-7 expression and not only increased proliferation and cell invasion but also increased the expression of p-Src and mitogen-activated protein kinase-extracellular signal-regulated kinase 1/2 that were suppressed upon claudin-7 overexpression. Of interest, CRC cell lines, which exhibited decreased claudin-7 expression, also exhibited promoter DNA hypermethylation, a modification associated with transcriptional silencing. Taken together, our data demonstrate a previously undescribed role of claudin-7 as a colon cancer suppressor and suggest that loss of claudin-7 potentiates EMT to promote colon cancer, in a manner dependent on Rab25. More... »
PAGES4570-4580
http://scigraph.springernature.com/pub.10.1038/onc.2014.385
DOIhttp://dx.doi.org/10.1038/onc.2014.385
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1019533174
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/25500541
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Adenocarcinoma",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Adenoma",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carcinogenesis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Claudins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Colonic Neoplasms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelial Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelial-Mesenchymal Transition",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HT29 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "MAP Kinase Signaling System",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Nude",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neoplasm Transplantation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transcriptome",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tumor Burden",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "rab GTP-Binding Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Veterans Affairs, Tennessee Valley Healthcare System, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Bhat",
"givenName": "A A",
"id": "sg:person.014174340300.75",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014174340300.75"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Veterans Affairs, Tennessee Valley Healthcare System, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Pathology, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Pope",
"givenName": "J L",
"id": "sg:person.01137726327.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137726327.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Smith",
"givenName": "J J",
"id": "sg:person.0746760561.80",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746760561.80"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Veterans Affairs, Tennessee Valley Healthcare System, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Ahmad",
"givenName": "R",
"id": "sg:person.01235164452.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235164452.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Chen",
"givenName": "X",
"id": "sg:person.0602643340.16",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602643340.16"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Biostatistics, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Washington",
"givenName": "M K",
"id": "sg:person.014213320717.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014213320717.05"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Beauchamp",
"givenName": "R D",
"id": "sg:person.01355254152.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355254152.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Singh",
"givenName": "A B",
"id": "sg:person.01123712251.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123712251.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Vanderbilt University Medical Center",
"id": "https://www.grid.ac/institutes/grid.412807.8",
"name": [
"Department of Veterans Affairs, Tennessee Valley Healthcare System, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA",
"Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA"
],
"type": "Organization"
},
"familyName": "Dhawan",
"givenName": "P",
"id": "sg:person.01031016226.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031016226.48"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1093/carcin/bgl120",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000872233"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2004.07.011",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001814625"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/ijc.24900",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002724641"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/ijc.24900",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002724641"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/0008-5472.can-06-1680",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004374475"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/j.gastro.2011.10.025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004887898"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.bjc.6605983",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005647140",
"https://doi.org/10.1038/sj.bjc.6605983"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cddis.2013.442",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006574152",
"https://doi.org/10.1038/cddis.2013.442"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci39675",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006800449"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci39675",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006800449"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1600-0854.2005.00362.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009088513"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1600-0854.2005.00362.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009088513"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci24543",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009178039"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1159/000124978",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010570886"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1091/mbc.10.1.47",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012567499"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2009.11.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017006433"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc865",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019149476",
"https://doi.org/10.1038/nrc865"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc865",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019149476",
"https://doi.org/10.1038/nrc865"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2559.2010.03685.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019575162"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2559.2010.03685.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019575162"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.oraloncology.2013.07.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019651472"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.yexcr.2011.05.019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019687407"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/ajpcell.00558.2003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021575246"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/321209a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022096390",
"https://doi.org/10.1038/321209a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/321209a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022096390",
"https://doi.org/10.1038/321209a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/321209a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022096390",
"https://doi.org/10.1038/321209a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1206199",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022257915",
"https://doi.org/10.1038/sj.onc.1206199"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1206199",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022257915",
"https://doi.org/10.1038/sj.onc.1206199"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.humpath.2006.11.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023143772"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/carcin/20.8.1425",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025076334"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg962",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027003214",
"https://doi.org/10.1038/nrg962"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg962",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027003214",
"https://doi.org/10.1038/nrg962"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/ijc.21739",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027118212"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/ijc.21739",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027118212"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.devcel.2007.08.012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029813019"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.100566997",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031726334"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci40728",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032521075"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci40728",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032521075"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1167/iovs.13-12988",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033145383"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/1940-6207.capr-08-0029",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034346272"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2353/ajpath.2007.060343",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035551970"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1369/jhc.6a6944.2006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035576154"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1369/jhc.6a6944.2006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035576154"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3727/096504001108747477",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036696017"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3727/096504001108747477",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036696017"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1136/gutjnl-2012-304241",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037547749"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.febslet.2005.09.091",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038313713"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.febslet.2005.09.091",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038313713"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/j.gastro.2011.08.038",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042136825"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/j.gastro.2009.11.005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043914495"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35067088",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044036068",
"https://doi.org/10.1038/35067088"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35067088",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044036068",
"https://doi.org/10.1038/35067088"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg1655",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044723213",
"https://doi.org/10.1038/nrg1655"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg1655",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044723213",
"https://doi.org/10.1038/nrg1655"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1242/jcs.01235",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046350952"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.humpath.2005.12.018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047600858"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.genet.30.1.441",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048623854"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.addr.2005.01.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050021104"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg816",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053491434",
"https://doi.org/10.1038/nrg816"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg816",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053491434",
"https://doi.org/10.1038/nrg816"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3892/or.2012.2161",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1071532176"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/cbt.4.4.1655",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072293722"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074546876",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074795204",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074801105",
"type": "CreativeWork"
}
],
"datePublished": "2015-08",
"datePublishedReg": "2015-08-01",
"description": "In normal colon, claudin-7 is one of the highly expressed claudin proteins and its knockdown in mice results in altered epithelial cell homeostasis and neonatal death. Notably, dysregulation of the epithelial homeostasis potentiates oncogenic transformation and growth. However, the role of claudin-7 in the regulation of colon tumorigenesis remains poorly understood. Using a large colorectal cancer (CRC) patient database and mouse models of colon cancer, we found claudin-7 expression to be significantly downregulated in cancer samples. Most notably, forced claudin-7 expression in poorly differentiated and highly metastatic SW620 colon cancer cells induced epithelial characteristics and inhibited their growth in soft agar and tumor growth in vivo. By contrast, knockdown of claudin-7 in HT-29 or DLD-1 cells induced epithelial-to-mesenchymal transition (EMT), colony formation, xenograft-tumor growth in athymic mice and invasion. Importantly, a claudin-7 signature gene profile generated by overlapping the DEGs (differentially expressed genes in a high-throughput transcriptome analysis using claudin-7-manipulated cells) with human claudin-7 signature genes identified high-risk CRC patients. Furthermore, Rab25, a colon cancer suppressor and regulator of the polarized cell trafficking constituted one of the highly upregulated DEGs in claudin-7 overexpressing cells. Notably, silencing of Rab25 expression counteracted the effects of claudin-7 expression and not only increased proliferation and cell invasion but also increased the expression of p-Src and mitogen-activated protein kinase-extracellular signal-regulated kinase 1/2 that were suppressed upon claudin-7 overexpression. Of interest, CRC cell lines, which exhibited decreased claudin-7 expression, also exhibited promoter DNA hypermethylation, a modification associated with transcriptional silencing. Taken together, our data demonstrate a previously undescribed role of claudin-7 as a colon cancer suppressor and suggest that loss of claudin-7 potentiates EMT to promote colon cancer, in a manner dependent on Rab25. ",
"genre": "research_article",
"id": "sg:pub.10.1038/onc.2014.385",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2401845",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2481416",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2623224",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2479093",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2472568",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2500180",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2438863",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2439027",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2440181",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1097543",
"issn": [
"0950-9232",
"1476-5594"
],
"name": "Oncogene",
"type": "Periodical"
},
{
"issueNumber": "35",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "34"
}
],
"name": "Claudin-7 expression induces mesenchymal to epithelial transformation (MET) to inhibit colon tumorigenesis",
"pagination": "4570-4580",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"5d119b52d55fa11e7e6f986639e8091ee6b391db7925cd64a697c73152906a94"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"25500541"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8711562"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/onc.2014.385"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1019533174"
]
}
],
"sameAs": [
"https://doi.org/10.1038/onc.2014.385",
"https://app.dimensions.ai/details/publication/pub.1019533174"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T22:19",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000424.jsonl",
"type": "ScholarlyArticle",
"url": "http://www.nature.com/onc/journal/v34/n35/full/onc2014385a.html"
}
]Download the RDF metadata as:Â json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/onc.2014.385'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/onc.2014.385'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/onc.2014.385'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/onc.2014.385'
This table displays all metadata directly associated to this object as RDF triples.
363 TRIPLES
21 PREDICATES
93 URIs
37 LITERALS
25 BLANK NODES