Straightening and sequential buckling of the pore-lining helices define the gating cycle of MscS View Full Text


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Article Info

DATE

2007-12

AUTHORS

Bradley Akitake, Andriy Anishkin, Naili Liu, Sergei Sukharev

ABSTRACT

We describe a mechanism connecting the adaptive behavior of the bacterial mechanosensitive channel MscS to the flexibility of the pore-lining helix TM3. Simulated expansion of the channel structure revealed straightening of a characteristic kink near Gly113 in the open state; return to the closed state produced an alternative kink at Gly121. Patch-clamp experiments showed that higher helical propensity introduced by a G113A mutation prevented inactivation. A similar mutation, G121A, kinetically impeded both closure and inactivation. Duplicating the glycines at each of these sites to increase flexibility produced directly opposite effects. The severely toxic G113A G121A mutation resulted in channels that could not inactivate or close with the release of tension. These data suggest that the open MscS features straight TM3 helices, which act as collapsible 'struts'. Closure and desensitization rely on buckling at Gly121, whereas the crystal-like kink at Gly113 is a feature of the inactivated state. More... »

PAGES

1141-1149

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1341

DOI

http://dx.doi.org/10.1038/nsmb1341

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049667487

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18037888


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