Homology-driven chromatin remodeling by human RAD54 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-05

AUTHORS

Zhaoqing Zhang, Hua-Ying Fan, Joseph A Goldman, Robert E Kingston

ABSTRACT

Human RAD51 and RAD54 are key players in homologous recombination, a process that requires homology recognition and strand invasion by a RAD51-single-stranded DNA (ssDNA) nucleoprotein filament and chromatin remodeling by RAD54. Here we use in vitro chromatin reconstitution systems to show that RAD51-ssDNA stimulates RAD54-dependent chromatin remodeling in a homology-dependent, polarity-independent manner. This stimulation was not seen with RAD54B or other remodelers. Chromatin remodeling by RAD54 enabled strand invasion by RAD51-ssDNA on nucleosomal templates, which was homology- and polarity-dependent. Three natural RAD54 mutants found in primary cancer cells showed specific defects in remodeling or in the RAD54-RAD51 interaction. We propose that RAD54 is recruited by RAD51-ssDNA filament to the chromatin of the intact chromosome and that it remodels that chromatin to facilitate accessibility for strand exchange. More... »

PAGES

397-405

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1223

DOI

http://dx.doi.org/10.1038/nsmb1223

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019139948

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17417655


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