The DEAD-box protein Ded1 unwinds RNA duplexes by a mode distinct from translocating helicases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-10-29

AUTHORS

Quansheng Yang, Eckhard Jankowsky

ABSTRACT

Helicases unwind RNA or DNA duplexes and displace proteins from nucleic acids in an ATP-dependent fashion. To unwind duplexes, helicases typically load onto one of the two nucleic acid strands, usually at a single-stranded region, and then translocate on this strand in a unidirectional fashion, thereby displacing the complementary DNA or RNA. Here we show that the DEAD-box RNA helicase Ded1 unwinds duplexes in a different manner. Ded1 uses the single-stranded region to gain access to the duplex. Strand separation is directly initiated from the duplex region and no covalent connection between the single strand and the duplex region is required. This new type of helicase activity explains observations with other DEAD-box proteins and may be the prototype for duplex-unwinding reactions in RNA metabolism. More... »

PAGES

981-986

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1165

DOI

http://dx.doi.org/10.1038/nsmb1165

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017398974

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17072313


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