A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-07

AUTHORS

Gail Miller, Steven Hahn

ABSTRACT

To directly map the position of promoter DNA within the RNA polymerase II (Pol II) transcription preinitiation complex (PIC), FeBABE was tethered to specific sites within the HIS4 promoter and used to map exposed surfaces of Pol II and the general transcription factors in proximity to DNA. Our results distinguish between previously proposed models for PIC structure and demonstrate that downstream promoter DNA is positioned over the central cleft of Pol II, with DNA upstream of TATA extending toward the Pol II subunit Rpb3. Also mapped were segments of TFIIB, TFIIE, TFIIF and TFIIH in proximity to promoter DNA. DNA downstream of the transcription bubble maps to a path between the two helicase subdomains of the TFIIH subunit Rad25 (also called XPB). Together, our results show how the general factors and Pol II converge on promoter DNA within the PIC. More... »

PAGES

603-610

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1117

DOI

http://dx.doi.org/10.1038/nsmb1117

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019909427

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16819517


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