Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-12

AUTHORS

Akio Yamashita, Tsung-Cheng Chang, Yukiko Yamashita, Wenmiao Zhu, Zhenping Zhong, Chyi-Ying A Chen, Ann-Bin Shyu

ABSTRACT

In mammalian cells, the enzymatic pathways involved in cytoplasmic mRNA decay are incompletely defined. In this study, we have used two approaches to disrupt activities of deadenylating and/or decapping enzymes to monitor effects on mRNA decay kinetics and trap decay intermediates. Our results show that deadenylation is the key first step that triggers decay of both wild-type stable and nonsense codon-containing unstable beta-globin mRNAs in mouse NIH3T3 fibroblasts. PAN2 and CCR4 are the major poly(A) nucleases active in cytoplasmic deadenylation that have biphasic kinetics, with PAN2 initiating deadenylation followed by CCR4-mediated poly(A) shortening. DCP2-mediated decapping takes place after deadenylation and may serve as a backup mechanism for triggering mRNA decay when initial deadenylation by PAN2 is compromised. Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay. More... »

PAGES

1054-1063

Journal

TITLE

Nature Structural & Molecular Biology

ISSUE

12

VOLUME

12

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nsmb1016

    DOI

    http://dx.doi.org/10.1038/nsmb1016

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039962565

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16284618


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