ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-11

AUTHORS

Christian Speck, Zhiqiang Chen, Huilin Li, Bruce Stillman

ABSTRACT

Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner. Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc6 to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase. The reaction is blocked by specific origin mutations that do not interfere with the interaction between ORC and DNA. Single-particle reconstruction of electron microscopic images shows that the ORC-Cdc6 complex forms a ring-shaped structure with dimensions similar to those of the ring-shaped MCM helicase. The ORC-Cdc6 structure is predicted to contain six AAA+ subunits, analogous to other ATP-dependent protein machines. We suggest that Cdc6 and origin DNA activate a molecular switch in ORC that contributes to pre-RC assembly. More... »

PAGES

965-971

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1002

DOI

http://dx.doi.org/10.1038/nsmb1002

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037802924

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16228006


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