ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-11

AUTHORS

Christian Speck, Zhiqiang Chen, Huilin Li, Bruce Stillman

ABSTRACT

Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner. Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc6 to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase. The reaction is blocked by specific origin mutations that do not interfere with the interaction between ORC and DNA. Single-particle reconstruction of electron microscopic images shows that the ORC-Cdc6 complex forms a ring-shaped structure with dimensions similar to those of the ring-shaped MCM helicase. The ORC-Cdc6 structure is predicted to contain six AAA+ subunits, analogous to other ATP-dependent protein machines. We suggest that Cdc6 and origin DNA activate a molecular switch in ORC that contributes to pre-RC assembly. More... »

PAGES

965-971

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb1002

DOI

http://dx.doi.org/10.1038/nsmb1002

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037802924

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16228006


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Footprinting", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophoretic Mobility Shift Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multiprotein Complexes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Origin Recognition Complex", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Structure-Activity Relationship", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cold Spring Harbor Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.225279.9", 
          "name": [
            "Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Speck", 
        "givenName": "Christian", 
        "id": "sg:person.01002033162.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002033162.19"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Chen", 
        "givenName": "Zhiqiang", 
        "id": "sg:person.0603055022.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603055022.34"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Li", 
        "givenName": "Huilin", 
        "id": "sg:person.014775475567.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014775475567.88"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Stillman", 
        "givenName": "Bruce", 
        "id": "sg:person.01030306604.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030306604.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.012578499", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002026132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90528-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002703626"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(00)00089-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002846115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-440x(99)00057-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004480596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.11.24.3375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007500329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/357128a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008093514", 
          "https://doi.org/10.1038/357128a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2004.12.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008183005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80110-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008479261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90096-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009264188"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.9.1.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009793423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012414621", 
          "https://doi.org/10.1038/nature01228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012414621", 
          "https://doi.org/10.1038/nature01228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.49.32421", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013085691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.1995.supplement_19.9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013712387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.11.5.1673", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017971766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(01)02203-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018141872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81889-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018836384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.201387198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020027201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m307392200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022217309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2004.04.045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025118095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.11.5611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025587960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.12.8291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025686279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m305531200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025802789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.7.11.1723", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026211784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jsbi.1999.4174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028456646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2003.10.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028892343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032128252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2004.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034703322"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/379180a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035350994", 
          "https://doi.org/10.1038/379180a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035997182", 
          "https://doi.org/10.1038/nsb893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035997182", 
          "https://doi.org/10.1038/nsb893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90427-r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036192395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.17.4774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036660133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.10540", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039725402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.081079298", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040081858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m307661200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040098928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.6.2224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040147166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.71.110601.135425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040856314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2818.1987.tb01333.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042754340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.20.9.3086-3096.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043938891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.69.1.829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045424909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.2.441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045939714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2003.tb00629.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047337294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.131006898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048601136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2001.4637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049259729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.26.14115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051988501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)00062-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052084733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/10445490050085933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059203595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1536007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062489660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074665009", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1995.tb07261.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082504755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb01057.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083023848"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-11", 
    "datePublishedReg": "2005-11-01", 
    "description": "Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner. Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc6 to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase. The reaction is blocked by specific origin mutations that do not interfere with the interaction between ORC and DNA. Single-particle reconstruction of electron microscopic images shows that the ORC-Cdc6 complex forms a ring-shaped structure with dimensions similar to those of the ring-shaped MCM helicase. The ORC-Cdc6 structure is predicted to contain six AAA+ subunits, analogous to other ATP-dependent protein machines. We suggest that Cdc6 and origin DNA activate a molecular switch in ORC that contributes to pre-RC assembly.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nsmb1002", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2518878", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2513405", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1295033", 
        "issn": [
          "1545-9993", 
          "2331-365X"
        ], 
        "name": "Nature Structural & Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA", 
    "pagination": "965-971", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b4154e21907d3b8ec05bd085f3c89ccdb764647583ad0ddbb317766e69c69799"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16228006"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101186374"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nsmb1002"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037802924"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nsmb1002", 
      "https://app.dimensions.ai/details/publication/pub.1037802924"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000360_0000000360/records_118339_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nsmb/journal/v12/n11/full/nsmb1002.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsmb1002'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsmb1002'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsmb1002'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsmb1002'


 

This table displays all metadata directly associated to this object as RDF triples.

308 TRIPLES      21 PREDICATES      95 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nsmb1002 schema:about N023757dec9a1464fa87ce030437c58f1
2 N25721e0ad7a041e6b12e9eb4fe828efb
3 N354f45d850814b58a382f1eea20b3cb1
4 N37caaff43dda49ed96167d551c25dead
5 N55048d505199469d9a4346d37db8c4bc
6 N79cc17a38dd44252a4fdda4b2ec1bafc
7 N827827dc94c74c6298eb9a585bd31d9a
8 N83947e0519934a518c224fd520d87aa2
9 N85750baafd6a4141bf4bdaabc9300597
10 Nb3409c9cb4ad48bd95412fc026f8d9d2
11 Nb3d7084548ab43b3a227a43eb421c90e
12 Nc52dc54f6d614f79a7091e7caf92cda3
13 Ne2969e9b3dda4e968b4a3d240515e446
14 Nf14c63b9051c4187bdd1a9a191bc190d
15 Nfc50d5bf9563475c90a2d425645ad3e2
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N882c83350cb748f993600da79e8257b7
19 schema:citation sg:pub.10.1038/35082600
20 sg:pub.10.1038/357128a0
21 sg:pub.10.1038/379180a0
22 sg:pub.10.1038/nature01228
23 sg:pub.10.1038/nsb893
24 https://app.dimensions.ai/details/publication/pub.1074665009
25 https://doi.org/10.1002/j.1460-2075.1995.tb07261.x
26 https://doi.org/10.1002/j.1460-2075.1996.tb01057.x
27 https://doi.org/10.1002/prot.10540
28 https://doi.org/10.1006/jmbi.2001.4637
29 https://doi.org/10.1006/jsbi.1999.4174
30 https://doi.org/10.1016/0092-8674(93)90427-r
31 https://doi.org/10.1016/0092-8674(95)90096-9
32 https://doi.org/10.1016/0092-8674(95)90528-6
33 https://doi.org/10.1016/j.febslet.2004.11.038
34 https://doi.org/10.1016/j.febslet.2004.12.011
35 https://doi.org/10.1016/j.jmb.2004.04.045
36 https://doi.org/10.1016/j.jsb.2003.10.010
37 https://doi.org/10.1016/j.molcel.2004.11.038
38 https://doi.org/10.1016/s0092-8674(00)81889-9
39 https://doi.org/10.1016/s0955-0674(00)00089-2
40 https://doi.org/10.1016/s0959-440x(99)00057-3
41 https://doi.org/10.1016/s0962-8924(01)02203-6
42 https://doi.org/10.1016/s1097-2765(00)00062-9
43 https://doi.org/10.1016/s1097-2765(00)80110-0
44 https://doi.org/10.1073/pnas.012578499
45 https://doi.org/10.1073/pnas.081079298
46 https://doi.org/10.1073/pnas.131006898
47 https://doi.org/10.1073/pnas.201387198
48 https://doi.org/10.1073/pnas.92.6.2224
49 https://doi.org/10.1073/pnas.94.11.5611
50 https://doi.org/10.1073/pnas.96.2.441
51 https://doi.org/10.1073/pnas.97.26.14115
52 https://doi.org/10.1074/jbc.273.49.32421
53 https://doi.org/10.1074/jbc.274.12.8291
54 https://doi.org/10.1074/jbc.m305531200
55 https://doi.org/10.1074/jbc.m307392200
56 https://doi.org/10.1074/jbc.m307661200
57 https://doi.org/10.1089/10445490050085933
58 https://doi.org/10.1091/mbc.11.5.1673
59 https://doi.org/10.1091/mbc.7.11.1723
60 https://doi.org/10.1091/mbc.9.1.63
61 https://doi.org/10.1093/emboj/19.17.4774
62 https://doi.org/10.1101/gad.11.24.3375
63 https://doi.org/10.1111/j.1365-2818.1987.tb01333.x
64 https://doi.org/10.1111/j.1574-6976.2003.tb00629.x
65 https://doi.org/10.1126/science.1536007
66 https://doi.org/10.1128/mcb.20.9.3086-3096.2000
67 https://doi.org/10.1146/annurev.biochem.69.1.829
68 https://doi.org/10.1146/annurev.biochem.71.110601.135425
69 https://doi.org/10.1242/jcs.1995.supplement_19.9
70 schema:datePublished 2005-11
71 schema:datePublishedReg 2005-11-01
72 schema:description Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner. Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc6 to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase. The reaction is blocked by specific origin mutations that do not interfere with the interaction between ORC and DNA. Single-particle reconstruction of electron microscopic images shows that the ORC-Cdc6 complex forms a ring-shaped structure with dimensions similar to those of the ring-shaped MCM helicase. The ORC-Cdc6 structure is predicted to contain six AAA+ subunits, analogous to other ATP-dependent protein machines. We suggest that Cdc6 and origin DNA activate a molecular switch in ORC that contributes to pre-RC assembly.
73 schema:genre research_article
74 schema:inLanguage en
75 schema:isAccessibleForFree true
76 schema:isPartOf N10e0ad9b70e14917a7a2e8c314ccd9ba
77 N9333146ebc5c4712ba96ed670ba42b5a
78 sg:journal.1295033
79 schema:name ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA
80 schema:pagination 965-971
81 schema:productId N7f569d4f0d13456d8d0ad00320c9af9b
82 N92c2441855424f2588d9c1b349055449
83 Na7514e7e88894d4387a20671079d5347
84 Nd09d9966dc2149c9a9f19a4406d64a8f
85 Nff188fcbc0214890b9cc7a81514ed2f0
86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037802924
87 https://doi.org/10.1038/nsmb1002
88 schema:sdDatePublished 2019-04-11T12:06
89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
90 schema:sdPublisher N87bf58ae2d314861b2a549e4ee6f392a
91 schema:url http://www.nature.com/nsmb/journal/v12/n11/full/nsmb1002.html
92 sgo:license sg:explorer/license/
93 sgo:sdDataset articles
94 rdf:type schema:ScholarlyArticle
95 N023757dec9a1464fa87ce030437c58f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Origin Recognition Complex
97 rdf:type schema:DefinedTerm
98 N10e0ad9b70e14917a7a2e8c314ccd9ba schema:volumeNumber 12
99 rdf:type schema:PublicationVolume
100 N195a78ad31d642259eda808455fcb8be rdf:first sg:person.0603055022.34
101 rdf:rest Nbac37adf92cc47fe9a824e310806eeb9
102 N25721e0ad7a041e6b12e9eb4fe828efb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name DNA Footprinting
104 rdf:type schema:DefinedTerm
105 N354f45d850814b58a382f1eea20b3cb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Cell Cycle Proteins
107 rdf:type schema:DefinedTerm
108 N37caaff43dda49ed96167d551c25dead schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Saccharomyces cerevisiae Proteins
110 rdf:type schema:DefinedTerm
111 N55048d505199469d9a4346d37db8c4bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Microscopy, Electron
113 rdf:type schema:DefinedTerm
114 N6d3ce505d65049f584208cd12fa63a29 rdf:first sg:person.01030306604.51
115 rdf:rest rdf:nil
116 N79cc17a38dd44252a4fdda4b2ec1bafc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Molecular Sequence Data
118 rdf:type schema:DefinedTerm
119 N7f569d4f0d13456d8d0ad00320c9af9b schema:name dimensions_id
120 schema:value pub.1037802924
121 rdf:type schema:PropertyValue
122 N827827dc94c74c6298eb9a585bd31d9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name DNA
124 rdf:type schema:DefinedTerm
125 N83947e0519934a518c224fd520d87aa2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Multiprotein Complexes
127 rdf:type schema:DefinedTerm
128 N85750baafd6a4141bf4bdaabc9300597 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Structure-Activity Relationship
130 rdf:type schema:DefinedTerm
131 N87bf58ae2d314861b2a549e4ee6f392a schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 N882c83350cb748f993600da79e8257b7 rdf:first sg:person.01002033162.19
134 rdf:rest N195a78ad31d642259eda808455fcb8be
135 N92c2441855424f2588d9c1b349055449 schema:name pubmed_id
136 schema:value 16228006
137 rdf:type schema:PropertyValue
138 N9333146ebc5c4712ba96ed670ba42b5a schema:issueNumber 11
139 rdf:type schema:PublicationIssue
140 Na7514e7e88894d4387a20671079d5347 schema:name doi
141 schema:value 10.1038/nsmb1002
142 rdf:type schema:PropertyValue
143 Nb3409c9cb4ad48bd95412fc026f8d9d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name DNA Primers
145 rdf:type schema:DefinedTerm
146 Nb3d7084548ab43b3a227a43eb421c90e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Saccharomyces cerevisiae
148 rdf:type schema:DefinedTerm
149 Nbac37adf92cc47fe9a824e310806eeb9 rdf:first sg:person.014775475567.88
150 rdf:rest N6d3ce505d65049f584208cd12fa63a29
151 Nc52dc54f6d614f79a7091e7caf92cda3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Mutation
153 rdf:type schema:DefinedTerm
154 Nd09d9966dc2149c9a9f19a4406d64a8f schema:name readcube_id
155 schema:value b4154e21907d3b8ec05bd085f3c89ccdb764647583ad0ddbb317766e69c69799
156 rdf:type schema:PropertyValue
157 Ne2969e9b3dda4e968b4a3d240515e446 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Models, Molecular
159 rdf:type schema:DefinedTerm
160 Nf14c63b9051c4187bdd1a9a191bc190d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Amino Acid Sequence
162 rdf:type schema:DefinedTerm
163 Nfc50d5bf9563475c90a2d425645ad3e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Electrophoretic Mobility Shift Assay
165 rdf:type schema:DefinedTerm
166 Nff188fcbc0214890b9cc7a81514ed2f0 schema:name nlm_unique_id
167 schema:value 101186374
168 rdf:type schema:PropertyValue
169 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
170 schema:name Biological Sciences
171 rdf:type schema:DefinedTerm
172 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
173 schema:name Biochemistry and Cell Biology
174 rdf:type schema:DefinedTerm
175 sg:grant.2513405 http://pending.schema.org/fundedItem sg:pub.10.1038/nsmb1002
176 rdf:type schema:MonetaryGrant
177 sg:grant.2518878 http://pending.schema.org/fundedItem sg:pub.10.1038/nsmb1002
178 rdf:type schema:MonetaryGrant
179 sg:journal.1295033 schema:issn 1545-9993
180 2331-365X
181 schema:name Nature Structural & Molecular Biology
182 rdf:type schema:Periodical
183 sg:person.01002033162.19 schema:affiliation https://www.grid.ac/institutes/grid.225279.9
184 schema:familyName Speck
185 schema:givenName Christian
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002033162.19
187 rdf:type schema:Person
188 sg:person.01030306604.51 schema:familyName Stillman
189 schema:givenName Bruce
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030306604.51
191 rdf:type schema:Person
192 sg:person.014775475567.88 schema:familyName Li
193 schema:givenName Huilin
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014775475567.88
195 rdf:type schema:Person
196 sg:person.0603055022.34 schema:familyName Chen
197 schema:givenName Zhiqiang
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603055022.34
199 rdf:type schema:Person
200 sg:pub.10.1038/35082600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036007845
201 https://doi.org/10.1038/35082600
202 rdf:type schema:CreativeWork
203 sg:pub.10.1038/357128a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008093514
204 https://doi.org/10.1038/357128a0
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/379180a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035350994
207 https://doi.org/10.1038/379180a0
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/nature01228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012414621
210 https://doi.org/10.1038/nature01228
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/nsb893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035997182
213 https://doi.org/10.1038/nsb893
214 rdf:type schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1074665009 schema:CreativeWork
216 https://doi.org/10.1002/j.1460-2075.1995.tb07261.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082504755
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1002/j.1460-2075.1996.tb01057.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1083023848
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1002/prot.10540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039725402
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1006/jmbi.2001.4637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049259729
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1006/jsbi.1999.4174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028456646
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/0092-8674(93)90427-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1036192395
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/0092-8674(95)90096-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009264188
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/0092-8674(95)90528-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002703626
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.febslet.2004.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034703322
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/j.febslet.2004.12.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008183005
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.jmb.2004.04.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025118095
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.jsb.2003.10.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028892343
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.molcel.2004.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032128252
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0092-8674(00)81889-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018836384
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0955-0674(00)00089-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002846115
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0959-440x(99)00057-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004480596
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s0962-8924(01)02203-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018141872
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s1097-2765(00)00062-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052084733
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s1097-2765(00)80110-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008479261
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1073/pnas.012578499 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002026132
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1073/pnas.081079298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040081858
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1073/pnas.131006898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048601136
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1073/pnas.201387198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020027201
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.92.6.2224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040147166
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1073/pnas.94.11.5611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025587960
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1073/pnas.96.2.441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045939714
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1073/pnas.97.26.14115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051988501
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1074/jbc.273.49.32421 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013085691
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1074/jbc.274.12.8291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025686279
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1074/jbc.m305531200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025802789
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1074/jbc.m307392200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022217309
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1074/jbc.m307661200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040098928
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1089/10445490050085933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059203595
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1091/mbc.11.5.1673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017971766
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1091/mbc.7.11.1723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026211784
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1091/mbc.9.1.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009793423
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1093/emboj/19.17.4774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036660133
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1101/gad.11.24.3375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007500329
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1111/j.1365-2818.1987.tb01333.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042754340
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1111/j.1574-6976.2003.tb00629.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047337294
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1126/science.1536007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062489660
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1128/mcb.20.9.3086-3096.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043938891
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1146/annurev.biochem.69.1.829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045424909
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1146/annurev.biochem.71.110601.135425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040856314
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1242/jcs.1995.supplement_19.9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013712387
305 rdf:type schema:CreativeWork
306 https://www.grid.ac/institutes/grid.225279.9 schema:alternateName Cold Spring Harbor Laboratory
307 schema:name Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.
308 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...