Ontology type: schema:ScholarlyArticle
2012-11
AUTHORSSiobhan Hughes, Frédéric Elustondo, Andrea Di Fonzo, Frédéric G Leroux, Ai C Wong, Ambrosius P Snijders, Stephen J Matthews, Peter Cherepanov
ABSTRACTCDC7 is a serine/threonine kinase that is essential for the initiation of eukaryotic DNA replication. CDC7 activity is controlled by its activator, DBF4. Here we present crystal structures of human CDC7-DBF4 in complex with a nucleotide or ATP-competing small molecules, revealing the active and inhibited forms of the kinase, respectively. DBF4 wraps around CDC7, burying approximately 6,000 Å(2) of hydrophobic molecular surface in a bipartite interface. The effector domain of DBF4, containing conserved motif C, is essential and sufficient to support CDC7 kinase activity by binding to the kinase N-terminal lobe and stabilizing its canonical αC helix. DBF4 motif M latches onto the C-terminal lobe of the kinase, acting as a tethering domain. Our results elucidate the structural basis for binding to and activation of CDC7 by DBF4 and provide a framework for the design of more potent and specific CDC7 inhibitors. More... »
PAGES1101
http://scigraph.springernature.com/pub.10.1038/nsmb.2404
DOIhttp://dx.doi.org/10.1038/nsmb.2404
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/23064647
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"url": "https://www.nature.com/articles/nsmb.2404"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsmb.2404'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsmb.2404'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsmb.2404'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsmb.2404'
This table displays all metadata directly associated to this object as RDF triples.
339 TRIPLES
21 PREDICATES
93 URIs
32 LITERALS
20 BLANK NODES