Alternative end-joining is suppressed by the canonical NHEJ component Xrcc4–ligase IV during chromosomal translocation formation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-04

AUTHORS

Deniz Simsek, Maria Jasin

ABSTRACT

Chromosomal translocations in hematologic and mesenchymal tumors form overwhelmingly by nonhomologous end-joining (NHEJ). Canonical NHEJ, essential for the repair of radiation-induced and some programmed double-strand breaks (DSBs), requires the Xrcc4-ligase IV complex. For other DSBs, the requirement for Xrcc4-ligase IV is less stringent, suggesting the existence of alternative end-joining (alt-NHEJ) pathways. To understand the contributions of the canonical NHEJ and alt-NHEJ pathways, we examined translocation formation in cells deficient in Xrcc4-ligase IV. We found that Xrcc4-ligase IV is not required for but rather suppresses translocations. Translocation breakpoint junctions have similar characteristics in wild-type cells and cells deficient in Xrcc4-ligase IV, including an unchanged bias toward microhomology, unlike what is observed for intrachromosomal DSB repair. Complex insertions in some junctions show that joining can be iterative, encompassing successive processing steps before joining. Our results imply that alt-NHEJ is the primary mediator of translocation formation in mammalian cells. More... »

PAGES

410

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb.1773

DOI

http://dx.doi.org/10.1038/nsmb.1773

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033094941

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20208544


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Ligase ATP", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Ligases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Repair", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Translocation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cornell University", 
          "id": "https://www.grid.ac/institutes/grid.5386.8", 
          "name": [
            "Developmental Biology Program, Memorial Sloan-Kettering Cancer Center and Weill Cornell Graduate School of Medical Sciences, New York, New York, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Simsek", 
        "givenName": "Deniz", 
        "id": "sg:person.01067453713.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067453713.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cornell University", 
          "id": "https://www.grid.ac/institutes/grid.5386.8", 
          "name": [
            "Developmental Biology Program, Memorial Sloan-Kettering Cancer Center and Weill Cornell Graduate School of Medical Sciences, New York, New York, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jasin", 
        "givenName": "Maria", 
        "id": "sg:person.01013505111.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013505111.17"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/nar/gkg317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001249078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001723874", 
          "https://doi.org/10.1038/nature08159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001723874", 
          "https://doi.org/10.1038/nature08159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.946401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002249237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/gcc.20693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003195556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/gcc.20693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003195556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.10.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003730897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2006.05.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004675666"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2005-06-2437", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005271873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20020851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005591897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.24.21.9305-9316.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009622574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010441359", 
          "https://doi.org/10.1038/nrm1202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010441359", 
          "https://doi.org/10.1038/nrm1202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkf452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010843889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.05.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011265918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014304085", 
          "https://doi.org/10.1038/sj.onc.1210871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81482-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014420338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2008.3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015186979", 
          "https://doi.org/10.1038/cr.2008.3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018450593", 
          "https://doi.org/10.1038/nrm1367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018450593", 
          "https://doi.org/10.1038/nrm1367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/26.23.5333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019887630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.25.3.896-906.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020496458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021023869", 
          "https://doi.org/10.1038/nature06020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkn094", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021908668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022283606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023613119", 
          "https://doi.org/10.1038/ng990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023613119", 
          "https://doi.org/10.1038/ng990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-04-3055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023688079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024106288", 
          "https://doi.org/10.1038/nature07054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.24.14405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024800636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.17.8929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025545125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.r700039200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026948241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0708541104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031736096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2776(06)94006-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032733707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.26.1.131-139.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032999521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2005.06.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033791086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.12.24.3831", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034752371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m610058200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034777211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.5.10.2599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035136336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/348649a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035885698", 
          "https://doi.org/10.1038/348649a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb950", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036115088", 
          "https://doi.org/10.1038/ncb950"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb950", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036115088", 
          "https://doi.org/10.1038/ncb950"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.11.5128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037441248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037900834", 
          "https://doi.org/10.1038/ncb1624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2007-07-104257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040286338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00770-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041575602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1521-4141(200203)32:3<701::aid-immu701>3.0.co;2-t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041712084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.22.14.5194-5202.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042327243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1444", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047444843", 
          "https://doi.org/10.1038/ncb1444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1444", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047444843", 
          "https://doi.org/10.1038/ncb1444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81714-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047629277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601559", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048799241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.11.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050400163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1164", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051551651", 
          "https://doi.org/10.1038/nrc1164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1164", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051551651", 
          "https://doi.org/10.1038/nrc1164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1591", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053450666", 
          "https://doi.org/10.1038/ncb1591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0921-8777(98)00063-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053716615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3579810", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070382153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074645506", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07997.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078025290"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-04", 
    "datePublishedReg": "2010-04-01", 
    "description": "Chromosomal translocations in hematologic and mesenchymal tumors form overwhelmingly by nonhomologous end-joining (NHEJ). Canonical NHEJ, essential for the repair of radiation-induced and some programmed double-strand breaks (DSBs), requires the Xrcc4-ligase IV complex. For other DSBs, the requirement for Xrcc4-ligase IV is less stringent, suggesting the existence of alternative end-joining (alt-NHEJ) pathways. To understand the contributions of the canonical NHEJ and alt-NHEJ pathways, we examined translocation formation in cells deficient in Xrcc4-ligase IV. We found that Xrcc4-ligase IV is not required for but rather suppresses translocations. Translocation breakpoint junctions have similar characteristics in wild-type cells and cells deficient in Xrcc4-ligase IV, including an unchanged bias toward microhomology, unlike what is observed for intrachromosomal DSB repair. Complex insertions in some junctions show that joining can be iterative, encompassing successive processing steps before joining. Our results imply that alt-NHEJ is the primary mediator of translocation formation in mammalian cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nsmb.1773", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2515092", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1295033", 
        "issn": [
          "1545-9993", 
          "2331-365X"
        ], 
        "name": "Nature Structural & Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "name": "Alternative end-joining is suppressed by the canonical NHEJ component Xrcc4\u2013ligase IV during chromosomal translocation formation", 
    "pagination": "410", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9da8cf93bc5a02b0181eff0cdb01d38999b3198699e665fc48811c8d8393c217"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20208544"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101186374"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nsmb.1773"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1033094941"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nsmb.1773", 
      "https://app.dimensions.ai/details/publication/pub.1033094941"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89816_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nsmb.1773"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1773'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1773'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1773'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1773'


 

This table displays all metadata directly associated to this object as RDF triples.

271 TRIPLES      21 PREDICATES      87 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nsmb.1773 schema:about N4a3c4c671b5243d1b59a14dcc7e4ad19
2 N51619c2837134933947aa0777c91c936
3 N8db8423cbf2448f39488286b112e07e2
4 N8ee0477da28a400386e92d2bb70d314b
5 Nf23cc54c551d4b619e873374018e8107
6 Nfedfdb6cd39e431bbb1fac4dea5d1f56
7 anzsrc-for:06
8 anzsrc-for:0601
9 schema:author Na19cbf0fe9bc4e1fba0046bb50d16588
10 schema:citation sg:pub.10.1038/348649a0
11 sg:pub.10.1038/cr.2008.3
12 sg:pub.10.1038/nature06020
13 sg:pub.10.1038/nature07054
14 sg:pub.10.1038/nature08159
15 sg:pub.10.1038/ncb1444
16 sg:pub.10.1038/ncb1591
17 sg:pub.10.1038/ncb1624
18 sg:pub.10.1038/ncb950
19 sg:pub.10.1038/ng990
20 sg:pub.10.1038/nrc1164
21 sg:pub.10.1038/nrm1202
22 sg:pub.10.1038/nrm1367
23 sg:pub.10.1038/sj.onc.1210871
24 https://app.dimensions.ai/details/publication/pub.1074645506
25 https://doi.org/10.1002/1521-4141(200203)32:3<701::aid-immu701>3.0.co;2-t
26 https://doi.org/10.1002/gcc.20693
27 https://doi.org/10.1002/j.1460-2075.1991.tb07997.x
28 https://doi.org/10.1016/j.dnarep.2006.05.020
29 https://doi.org/10.1016/j.molcel.2004.05.008
30 https://doi.org/10.1016/j.molcel.2004.10.029
31 https://doi.org/10.1016/j.molcel.2004.11.017
32 https://doi.org/10.1016/j.molcel.2005.06.012
33 https://doi.org/10.1016/s0065-2776(06)94006-1
34 https://doi.org/10.1016/s0092-8674(00)81482-8
35 https://doi.org/10.1016/s0092-8674(00)81714-6
36 https://doi.org/10.1016/s0092-8674(02)00770-5
37 https://doi.org/10.1016/s0921-8777(98)00063-9
38 https://doi.org/10.1038/sj.emboj.7600375
39 https://doi.org/10.1038/sj.emboj.7601559
40 https://doi.org/10.1073/pnas.0708541104
41 https://doi.org/10.1073/pnas.89.11.5128
42 https://doi.org/10.1073/pnas.93.17.8929
43 https://doi.org/10.1074/jbc.271.24.14405
44 https://doi.org/10.1074/jbc.m610058200
45 https://doi.org/10.1074/jbc.r700039200
46 https://doi.org/10.1084/jem.20020851
47 https://doi.org/10.1093/nar/26.23.5333
48 https://doi.org/10.1093/nar/gkf452
49 https://doi.org/10.1093/nar/gkg317
50 https://doi.org/10.1093/nar/gkn094
51 https://doi.org/10.1101/gad.12.24.3831
52 https://doi.org/10.1101/gad.946401
53 https://doi.org/10.1128/mcb.22.14.5194-5202.2002
54 https://doi.org/10.1128/mcb.24.21.9305-9316.2004
55 https://doi.org/10.1128/mcb.25.3.896-906.2005
56 https://doi.org/10.1128/mcb.26.1.131-139.2006
57 https://doi.org/10.1128/mcb.5.10.2599
58 https://doi.org/10.1158/0008-5472.can-04-3055
59 https://doi.org/10.1182/blood-2005-06-2437
60 https://doi.org/10.1182/blood-2007-07-104257
61 https://doi.org/10.2307/3579810
62 schema:datePublished 2010-04
63 schema:datePublishedReg 2010-04-01
64 schema:description Chromosomal translocations in hematologic and mesenchymal tumors form overwhelmingly by nonhomologous end-joining (NHEJ). Canonical NHEJ, essential for the repair of radiation-induced and some programmed double-strand breaks (DSBs), requires the Xrcc4-ligase IV complex. For other DSBs, the requirement for Xrcc4-ligase IV is less stringent, suggesting the existence of alternative end-joining (alt-NHEJ) pathways. To understand the contributions of the canonical NHEJ and alt-NHEJ pathways, we examined translocation formation in cells deficient in Xrcc4-ligase IV. We found that Xrcc4-ligase IV is not required for but rather suppresses translocations. Translocation breakpoint junctions have similar characteristics in wild-type cells and cells deficient in Xrcc4-ligase IV, including an unchanged bias toward microhomology, unlike what is observed for intrachromosomal DSB repair. Complex insertions in some junctions show that joining can be iterative, encompassing successive processing steps before joining. Our results imply that alt-NHEJ is the primary mediator of translocation formation in mammalian cells.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree true
68 schema:isPartOf N534de2d03e4547eeb01482371ba92f6e
69 Nff0abacb461b4ea4bba6f01650aff288
70 sg:journal.1295033
71 schema:name Alternative end-joining is suppressed by the canonical NHEJ component Xrcc4–ligase IV during chromosomal translocation formation
72 schema:pagination 410
73 schema:productId N41c4e0c35b91430b8928dbd3823518e3
74 N4808f144da794823bce502d376c6d2b1
75 N8e201479393f4305a6d3e54b026e8eda
76 Naf7dc3a15e93442abb084430dde571ea
77 Nf1d0066262854f378b0e94276863e62c
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033094941
79 https://doi.org/10.1038/nsmb.1773
80 schema:sdDatePublished 2019-04-11T10:00
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher N422e48268a7341de9ece77f47585910e
83 schema:url https://www.nature.com/articles/nsmb.1773
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N2de2ab4cf5a2422688d777d04438c188 rdf:first sg:person.01013505111.17
88 rdf:rest rdf:nil
89 N41c4e0c35b91430b8928dbd3823518e3 schema:name dimensions_id
90 schema:value pub.1033094941
91 rdf:type schema:PropertyValue
92 N422e48268a7341de9ece77f47585910e schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N4808f144da794823bce502d376c6d2b1 schema:name nlm_unique_id
95 schema:value 101186374
96 rdf:type schema:PropertyValue
97 N4a3c4c671b5243d1b59a14dcc7e4ad19 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Translocation, Genetic
99 rdf:type schema:DefinedTerm
100 N51619c2837134933947aa0777c91c936 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Humans
102 rdf:type schema:DefinedTerm
103 N534de2d03e4547eeb01482371ba92f6e schema:volumeNumber 17
104 rdf:type schema:PublicationVolume
105 N8db8423cbf2448f39488286b112e07e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name DNA-Binding Proteins
107 rdf:type schema:DefinedTerm
108 N8e201479393f4305a6d3e54b026e8eda schema:name doi
109 schema:value 10.1038/nsmb.1773
110 rdf:type schema:PropertyValue
111 N8ee0477da28a400386e92d2bb70d314b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name DNA Repair
113 rdf:type schema:DefinedTerm
114 Na19cbf0fe9bc4e1fba0046bb50d16588 rdf:first sg:person.01067453713.16
115 rdf:rest N2de2ab4cf5a2422688d777d04438c188
116 Naf7dc3a15e93442abb084430dde571ea schema:name readcube_id
117 schema:value 9da8cf93bc5a02b0181eff0cdb01d38999b3198699e665fc48811c8d8393c217
118 rdf:type schema:PropertyValue
119 Nf1d0066262854f378b0e94276863e62c schema:name pubmed_id
120 schema:value 20208544
121 rdf:type schema:PropertyValue
122 Nf23cc54c551d4b619e873374018e8107 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name DNA Ligase ATP
124 rdf:type schema:DefinedTerm
125 Nfedfdb6cd39e431bbb1fac4dea5d1f56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name DNA Ligases
127 rdf:type schema:DefinedTerm
128 Nff0abacb461b4ea4bba6f01650aff288 schema:issueNumber 4
129 rdf:type schema:PublicationIssue
130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biological Sciences
132 rdf:type schema:DefinedTerm
133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
134 schema:name Biochemistry and Cell Biology
135 rdf:type schema:DefinedTerm
136 sg:grant.2515092 http://pending.schema.org/fundedItem sg:pub.10.1038/nsmb.1773
137 rdf:type schema:MonetaryGrant
138 sg:journal.1295033 schema:issn 1545-9993
139 2331-365X
140 schema:name Nature Structural & Molecular Biology
141 rdf:type schema:Periodical
142 sg:person.01013505111.17 schema:affiliation https://www.grid.ac/institutes/grid.5386.8
143 schema:familyName Jasin
144 schema:givenName Maria
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013505111.17
146 rdf:type schema:Person
147 sg:person.01067453713.16 schema:affiliation https://www.grid.ac/institutes/grid.5386.8
148 schema:familyName Simsek
149 schema:givenName Deniz
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067453713.16
151 rdf:type schema:Person
152 sg:pub.10.1038/348649a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035885698
153 https://doi.org/10.1038/348649a0
154 rdf:type schema:CreativeWork
155 sg:pub.10.1038/cr.2008.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015186979
156 https://doi.org/10.1038/cr.2008.3
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/nature06020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021023869
159 https://doi.org/10.1038/nature06020
160 rdf:type schema:CreativeWork
161 sg:pub.10.1038/nature07054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024106288
162 https://doi.org/10.1038/nature07054
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/nature08159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001723874
165 https://doi.org/10.1038/nature08159
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/ncb1444 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047444843
168 https://doi.org/10.1038/ncb1444
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/ncb1591 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053450666
171 https://doi.org/10.1038/ncb1591
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/ncb1624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037900834
174 https://doi.org/10.1038/ncb1624
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/ncb950 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036115088
177 https://doi.org/10.1038/ncb950
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/ng990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023613119
180 https://doi.org/10.1038/ng990
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nrc1164 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051551651
183 https://doi.org/10.1038/nrc1164
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/nrm1202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010441359
186 https://doi.org/10.1038/nrm1202
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/nrm1367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018450593
189 https://doi.org/10.1038/nrm1367
190 rdf:type schema:CreativeWork
191 sg:pub.10.1038/sj.onc.1210871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014304085
192 https://doi.org/10.1038/sj.onc.1210871
193 rdf:type schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1074645506 schema:CreativeWork
195 https://doi.org/10.1002/1521-4141(200203)32:3<701::aid-immu701>3.0.co;2-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1041712084
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1002/gcc.20693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003195556
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1002/j.1460-2075.1991.tb07997.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078025290
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.dnarep.2006.05.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004675666
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.molcel.2004.05.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011265918
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.molcel.2004.10.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003730897
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.molcel.2004.11.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050400163
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.molcel.2005.06.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033791086
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0065-2776(06)94006-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032733707
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/s0092-8674(00)81482-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014420338
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0092-8674(00)81714-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047629277
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0092-8674(02)00770-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041575602
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s0921-8777(98)00063-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053716615
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1038/sj.emboj.7600375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022283606
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1038/sj.emboj.7601559 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048799241
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.0708541104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031736096
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.89.11.5128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037441248
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1073/pnas.93.17.8929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025545125
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1074/jbc.271.24.14405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024800636
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/jbc.m610058200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034777211
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1074/jbc.r700039200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026948241
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1084/jem.20020851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005591897
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/nar/26.23.5333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019887630
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/nar/gkf452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010843889
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/nar/gkg317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001249078
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/nar/gkn094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021908668
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1101/gad.12.24.3831 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034752371
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1101/gad.946401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002249237
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1128/mcb.22.14.5194-5202.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042327243
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/mcb.24.21.9305-9316.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009622574
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1128/mcb.25.3.896-906.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020496458
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1128/mcb.26.1.131-139.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032999521
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1128/mcb.5.10.2599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035136336
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1158/0008-5472.can-04-3055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023688079
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1182/blood-2005-06-2437 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005271873
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1182/blood-2007-07-104257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040286338
266 rdf:type schema:CreativeWork
267 https://doi.org/10.2307/3579810 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070382153
268 rdf:type schema:CreativeWork
269 https://www.grid.ac/institutes/grid.5386.8 schema:alternateName Cornell University
270 schema:name Developmental Biology Program, Memorial Sloan-Kettering Cancer Center and Weill Cornell Graduate School of Medical Sciences, New York, New York, USA.
271 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...