Strong cooperativity between subunits in voltage-gated proton channels View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-01

AUTHORS

Carlos Gonzalez, Hans P Koch, Ben M Drum, H Peter Larsson

ABSTRACT

Voltage-activated proton (Hv) channels are essential components in the innate immune response. Hv channels are dimeric proteins with one proton permeation pathway per subunit. It is unknown how Hv channels are activated by voltage and whether there is any cooperation between subunits during voltage activation. Using cysteine accessibility measurements and voltage-clamp fluorometry, we show data consistent with the possibility that the fourth transmembrane segment S4 functions as the voltage sensor in Ciona intestinalis Hv channels. Unexpectedly, in a dimeric Hv channel, the S4 in both subunits must move to activate the two proton permeation pathways. In contrast, if Hv subunits are prevented from dimerizing, the movement of a single S4 is sufficient to activate the proton permeation pathway in a subunit. These results indicate strong cooperativity between subunits in dimeric Hv channels. More... »

PAGES

51-56

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb.1739

DOI

http://dx.doi.org/10.1038/nsmb.1739

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035760529

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20023639


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