Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-08

AUTHORS

Zhiwei Huang, Sarah E Sutton, Adam J Wallenfang, Robert C Orchard, Xiaojing Wu, Yingcai Feng, Jijie Chai, Neal M Alto

ABSTRACT

The Escherichia coli type III effector Map belongs to a large family of bacterial virulence factors that activate host Rho GTPase signaling pathways through an unknown molecular mechanism. Here we report direct evidence that Map functions as a potent and selective guanine-nucleotide exchange factor (GEF) for Cdc42. The 2.3-A structure of the Map-Cdc42 complex revealed that Map mimics the GEF strategy of the mammalian Dbl family but has a three-dimensional architecture that is nearly identical to the bacterial GEF Salmonella spp. SopE. A comparative analysis between human and bacterial GEFs revealed a previously uncharacterized pairing mechanism between Map and the variable beta2-3 interswitch region of Cdc42. We propose a GTPase selection model that is experimentally validated by the preferential activation Rac1 and RhoA by the Shigella spp. effectors IpgB1 and IpgB2, respectively. These results significantly expand the repertoire of bacterial GEF mimics and unify a GEF selection mechanism for host GTPase substrates. More... »

PAGES

853-860

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsmb.1647

DOI

http://dx.doi.org/10.1038/nsmb.1647

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021154968

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19620963


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding, Competitive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanine Nucleotide Exchange Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "cdc42 GTP-Binding Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "rho GTP-Binding Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Zhiwei", 
        "id": "sg:person.012706151271.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012706151271.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sutton", 
        "givenName": "Sarah E", 
        "id": "sg:person.0727645116.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727645116.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wallenfang", 
        "givenName": "Adam J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Orchard", 
        "givenName": "Robert C", 
        "id": "sg:person.01160647627.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160647627.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Xiaojing", 
        "id": "sg:person.01132201355.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132201355.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feng", 
        "givenName": "Yingcai", 
        "id": "sg:person.01151175613.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151175613.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China.", 
            "Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chai", 
        "givenName": "Jijie", 
        "id": "sg:person.01342040611.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342040611.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alto", 
        "givenName": "Neal M", 
        "id": "sg:person.01323614335.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323614335.66"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/316273a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000554641", 
          "https://doi.org/10.1038/316273a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(95)56011-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001885162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0909049503023938", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003067022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)76066-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004907170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2005.02.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005801037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2007.11.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006014775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-5822.2008.01254.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006394094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m502509200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008711773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb796", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008933904", 
          "https://doi.org/10.1038/nsb796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1110225", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012847668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(04)00003-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013496682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0108767390010224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014210169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200705021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016209052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35047014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016895952", 
          "https://doi.org/10.1038/35047014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35047014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016895952", 
          "https://doi.org/10.1038/35047014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017893678", 
          "https://doi.org/10.1038/ncb1526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017893678", 
          "https://doi.org/10.1038/ncb1526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444998003254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019779527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdf329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019829073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb719", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021135221", 
          "https://doi.org/10.1038/nsb719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1067025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024140312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1462-5822.2000.00082.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024476138"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444994003112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025573949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/4209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027218113", 
          "https://doi.org/10.1038/4209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-0854.2001.20907.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027630227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2004.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029440917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1062023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033212920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2002.02952.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033221175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2006.11.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035540369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(95)56003-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035685402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-5822.2008.01136.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038278115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m410820200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038634308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.10.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041736927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2006.05109.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042603055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2006.05109.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042603055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.21.020604.150721", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042781454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1996.tb02497.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043021690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.13.3235", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043175432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81442-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044053295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/21.6.1315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045395479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chom.2008.08.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045443968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/354311a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050462145", 
          "https://doi.org/10.1038/354311a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079343524", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-08", 
    "datePublishedReg": "2009-08-01", 
    "description": "The Escherichia coli type III effector Map belongs to a large family of bacterial virulence factors that activate host Rho GTPase signaling pathways through an unknown molecular mechanism. Here we report direct evidence that Map functions as a potent and selective guanine-nucleotide exchange factor (GEF) for Cdc42. The 2.3-A structure of the Map-Cdc42 complex revealed that Map mimics the GEF strategy of the mammalian Dbl family but has a three-dimensional architecture that is nearly identical to the bacterial GEF Salmonella spp. SopE. A comparative analysis between human and bacterial GEFs revealed a previously uncharacterized pairing mechanism between Map and the variable beta2-3 interswitch region of Cdc42. We propose a GTPase selection model that is experimentally validated by the preferential activation Rac1 and RhoA by the Shigella spp. effectors IpgB1 and IpgB2, respectively. These results significantly expand the repertoire of bacterial GEF mimics and unify a GEF selection mechanism for host GTPase substrates.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nsmb.1647", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2458697", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1295033", 
        "issn": [
          "1545-9993", 
          "2331-365X"
        ], 
        "name": "Nature Structural & Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "name": "Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics", 
    "pagination": "853-860", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "64cb19129146fe14ee8e01dabd962e8b0cae3c48c5723faa7e9d86d6d17eb97a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19620963"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101186374"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nsmb.1647"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021154968"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nsmb.1647", 
      "https://app.dimensions.ai/details/publication/pub.1021154968"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000345_0000000345/records_64111_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/nsmb.1647"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1647'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1647'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1647'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsmb.1647'


 

This table displays all metadata directly associated to this object as RDF triples.

328 TRIPLES      21 PREDICATES      88 URIs      40 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nsmb.1647 schema:about N12d65f9ce36a46aca15eae06308f8627
2 N131ef051bd2149c8a7e346e67b4b9300
3 N1fdbac8626604fc4ba2960f3a070e36a
4 N31a093e2397c45499aa781e9e2655837
5 N40a0bcde2e214bbd9ec8804143b8d90e
6 N540b2f0000ad48108cda4f398e14d4ed
7 N5e96a6152ea948eeb74a0566a9ab1059
8 N6ea7abaffde347bea37a28abde19e5f4
9 N76e05e87962941228b6ac32e899f6c50
10 Nb0e8f4ff9656402ea46b3922eda085ca
11 Nbe6d476f49eb49f1ad07563656dac4f8
12 Nc8547f8b622241238da9036ebc227864
13 Ndf1ed08f82ec49fb95fb3ac435e13f02
14 Ne260809b2d7841c88714bc780a12bbd7
15 Ne65743944bd5446e9e74edf1250373d2
16 Ne896a2609d5540bf9e2a4f1ffef83489
17 Nf7a0a4eb6e524d0683516551c2da5fbd
18 Nfb86ac32b4014825bc9f468c356b4b76
19 Nfe1e3b01a18d4c47aca36a782af26150
20 anzsrc-for:11
21 anzsrc-for:1108
22 schema:author N4164369bfa434a13acdc67bafc8cecf4
23 schema:citation sg:pub.10.1038/316273a0
24 sg:pub.10.1038/35047014
25 sg:pub.10.1038/354311a0
26 sg:pub.10.1038/4209
27 sg:pub.10.1038/ncb1526
28 sg:pub.10.1038/nsb719
29 sg:pub.10.1038/nsb796
30 https://app.dimensions.ai/details/publication/pub.1079343524
31 https://doi.org/10.1016/0076-6879(95)56003-3
32 https://doi.org/10.1016/0076-6879(95)56011-4
33 https://doi.org/10.1016/j.cell.2005.10.031
34 https://doi.org/10.1016/j.chom.2008.08.012
35 https://doi.org/10.1016/j.micinf.2007.11.011
36 https://doi.org/10.1016/j.str.2004.09.003
37 https://doi.org/10.1016/j.tcb.2006.11.004
38 https://doi.org/10.1016/j.tibs.2005.02.002
39 https://doi.org/10.1016/s0076-6879(97)76066-x
40 https://doi.org/10.1016/s0092-8674(00)81442-7
41 https://doi.org/10.1016/s0092-8674(04)00003-0
42 https://doi.org/10.1034/j.1600-0854.2001.20907.x
43 https://doi.org/10.1046/j.1365-2958.2002.02952.x
44 https://doi.org/10.1046/j.1462-5822.2000.00082.x
45 https://doi.org/10.1074/jbc.m410820200
46 https://doi.org/10.1074/jbc.m502509200
47 https://doi.org/10.1083/jcb.200705021
48 https://doi.org/10.1093/emboj/19.13.3235
49 https://doi.org/10.1093/emboj/21.6.1315
50 https://doi.org/10.1093/emboj/cdf329
51 https://doi.org/10.1107/s0108767390010224
52 https://doi.org/10.1107/s0907444994003112
53 https://doi.org/10.1107/s0907444998003254
54 https://doi.org/10.1107/s0909049503023938
55 https://doi.org/10.1111/j.1365-2958.1996.tb02497.x
56 https://doi.org/10.1111/j.1365-2958.2006.05109.x
57 https://doi.org/10.1111/j.1462-5822.2008.01136.x
58 https://doi.org/10.1111/j.1462-5822.2008.01254.x
59 https://doi.org/10.1126/science.1062023
60 https://doi.org/10.1126/science.1067025
61 https://doi.org/10.1126/science.1110225
62 https://doi.org/10.1146/annurev.cellbio.21.020604.150721
63 schema:datePublished 2009-08
64 schema:datePublishedReg 2009-08-01
65 schema:description The Escherichia coli type III effector Map belongs to a large family of bacterial virulence factors that activate host Rho GTPase signaling pathways through an unknown molecular mechanism. Here we report direct evidence that Map functions as a potent and selective guanine-nucleotide exchange factor (GEF) for Cdc42. The 2.3-A structure of the Map-Cdc42 complex revealed that Map mimics the GEF strategy of the mammalian Dbl family but has a three-dimensional architecture that is nearly identical to the bacterial GEF Salmonella spp. SopE. A comparative analysis between human and bacterial GEFs revealed a previously uncharacterized pairing mechanism between Map and the variable beta2-3 interswitch region of Cdc42. We propose a GTPase selection model that is experimentally validated by the preferential activation Rac1 and RhoA by the Shigella spp. effectors IpgB1 and IpgB2, respectively. These results significantly expand the repertoire of bacterial GEF mimics and unify a GEF selection mechanism for host GTPase substrates.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree true
69 schema:isPartOf N14235d58aea34ca389532381226fb669
70 N74350df00f88414193fcc91e99a2eaca
71 sg:journal.1295033
72 schema:name Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics
73 schema:pagination 853-860
74 schema:productId N07004612eba545b9a2777dbbcb976232
75 N34a18289ac204d3683eb56a290fc191e
76 N44c083792f2f4e5696fdbe9ba357a880
77 N9c29fea2a883403d9b7b700ecc9a9b05
78 Neb78198e2f7e4e9d878e91d7a53f1bd3
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021154968
80 https://doi.org/10.1038/nsmb.1647
81 schema:sdDatePublished 2019-04-11T09:25
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher Nfcb1a8f8613c45a7837bbf473d8b5813
84 schema:url http://www.nature.com/articles/nsmb.1647
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N07004612eba545b9a2777dbbcb976232 schema:name doi
89 schema:value 10.1038/nsmb.1647
90 rdf:type schema:PropertyValue
91 N12d65f9ce36a46aca15eae06308f8627 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Molecular Sequence Data
93 rdf:type schema:DefinedTerm
94 N131ef051bd2149c8a7e346e67b4b9300 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Binding Sites
96 rdf:type schema:DefinedTerm
97 N14235d58aea34ca389532381226fb669 schema:issueNumber 8
98 rdf:type schema:PublicationIssue
99 N1fdbac8626604fc4ba2960f3a070e36a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Amino Acid Sequence
101 rdf:type schema:DefinedTerm
102 N2eab22b229844c8c8bea6a00e0a7fe3b rdf:first sg:person.01342040611.97
103 rdf:rest Na06d0b50e502436cbb9e49ebd8c25bbc
104 N31a093e2397c45499aa781e9e2655837 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Crystallization
106 rdf:type schema:DefinedTerm
107 N34a18289ac204d3683eb56a290fc191e schema:name nlm_unique_id
108 schema:value 101186374
109 rdf:type schema:PropertyValue
110 N3e84799843a84aa48c02485b46ebe33a rdf:first sg:person.01132201355.84
111 rdf:rest Nc5e05dee22fa49f783a5ac1bc818a127
112 N40a0bcde2e214bbd9ec8804143b8d90e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Guanine Nucleotide Exchange Factors
114 rdf:type schema:DefinedTerm
115 N4164369bfa434a13acdc67bafc8cecf4 rdf:first sg:person.012706151271.73
116 rdf:rest Nf24848de0f7f420cba5ba84e1d520c27
117 N44c083792f2f4e5696fdbe9ba357a880 schema:name dimensions_id
118 schema:value pub.1021154968
119 rdf:type schema:PropertyValue
120 N540b2f0000ad48108cda4f398e14d4ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Transfection
122 rdf:type schema:DefinedTerm
123 N5e96a6152ea948eeb74a0566a9ab1059 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Recombinant Fusion Proteins
125 rdf:type schema:DefinedTerm
126 N6577a9a46e544f338b892d102ef72391 rdf:first sg:person.01160647627.53
127 rdf:rest N3e84799843a84aa48c02485b46ebe33a
128 N6ea7abaffde347bea37a28abde19e5f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Protein Conformation
130 rdf:type schema:DefinedTerm
131 N74350df00f88414193fcc91e99a2eaca schema:volumeNumber 16
132 rdf:type schema:PublicationVolume
133 N76e05e87962941228b6ac32e899f6c50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name cdc42 GTP-Binding Protein
135 rdf:type schema:DefinedTerm
136 N9c29fea2a883403d9b7b700ecc9a9b05 schema:name pubmed_id
137 schema:value 19620963
138 rdf:type schema:PropertyValue
139 Na06d0b50e502436cbb9e49ebd8c25bbc rdf:first sg:person.01323614335.66
140 rdf:rest rdf:nil
141 Nb0b641dc950a4e9e9f3e96a6acef8760 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
142 schema:familyName Wallenfang
143 schema:givenName Adam J
144 rdf:type schema:Person
145 Nb0e8f4ff9656402ea46b3922eda085ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Green Fluorescent Proteins
147 rdf:type schema:DefinedTerm
148 Nbe6d476f49eb49f1ad07563656dac4f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name rho GTP-Binding Proteins
150 rdf:type schema:DefinedTerm
151 Nc5e05dee22fa49f783a5ac1bc818a127 rdf:first sg:person.01151175613.73
152 rdf:rest N2eab22b229844c8c8bea6a00e0a7fe3b
153 Nc8547f8b622241238da9036ebc227864 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Cell Line
155 rdf:type schema:DefinedTerm
156 Ncd8e396740084e5b92ac471822015e85 rdf:first Nb0b641dc950a4e9e9f3e96a6acef8760
157 rdf:rest N6577a9a46e544f338b892d102ef72391
158 Ndf1ed08f82ec49fb95fb3ac435e13f02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Sequence Homology, Amino Acid
160 rdf:type schema:DefinedTerm
161 Ne260809b2d7841c88714bc780a12bbd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Binding, Competitive
163 rdf:type schema:DefinedTerm
164 Ne65743944bd5446e9e74edf1250373d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Escherichia coli Proteins
166 rdf:type schema:DefinedTerm
167 Ne896a2609d5540bf9e2a4f1ffef83489 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Protein Structure, Tertiary
169 rdf:type schema:DefinedTerm
170 Neb78198e2f7e4e9d878e91d7a53f1bd3 schema:name readcube_id
171 schema:value 64cb19129146fe14ee8e01dabd962e8b0cae3c48c5723faa7e9d86d6d17eb97a
172 rdf:type schema:PropertyValue
173 Nf24848de0f7f420cba5ba84e1d520c27 rdf:first sg:person.0727645116.99
174 rdf:rest Ncd8e396740084e5b92ac471822015e85
175 Nf7a0a4eb6e524d0683516551c2da5fbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Models, Molecular
177 rdf:type schema:DefinedTerm
178 Nfb86ac32b4014825bc9f468c356b4b76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Humans
180 rdf:type schema:DefinedTerm
181 Nfcb1a8f8613c45a7837bbf473d8b5813 schema:name Springer Nature - SN SciGraph project
182 rdf:type schema:Organization
183 Nfe1e3b01a18d4c47aca36a782af26150 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Protein Binding
185 rdf:type schema:DefinedTerm
186 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
187 schema:name Medical and Health Sciences
188 rdf:type schema:DefinedTerm
189 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
190 schema:name Medical Microbiology
191 rdf:type schema:DefinedTerm
192 sg:grant.2458697 http://pending.schema.org/fundedItem sg:pub.10.1038/nsmb.1647
193 rdf:type schema:MonetaryGrant
194 sg:journal.1295033 schema:issn 1545-9993
195 2331-365X
196 schema:name Nature Structural & Molecular Biology
197 rdf:type schema:Periodical
198 sg:person.01132201355.84 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
199 schema:familyName Wu
200 schema:givenName Xiaojing
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132201355.84
202 rdf:type schema:Person
203 sg:person.01151175613.73 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
204 schema:familyName Feng
205 schema:givenName Yingcai
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151175613.73
207 rdf:type schema:Person
208 sg:person.01160647627.53 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
209 schema:familyName Orchard
210 schema:givenName Robert C
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160647627.53
212 rdf:type schema:Person
213 sg:person.012706151271.73 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
214 schema:familyName Huang
215 schema:givenName Zhiwei
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012706151271.73
217 rdf:type schema:Person
218 sg:person.01323614335.66 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
219 schema:familyName Alto
220 schema:givenName Neal M
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323614335.66
222 rdf:type schema:Person
223 sg:person.01342040611.97 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
224 schema:familyName Chai
225 schema:givenName Jijie
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342040611.97
227 rdf:type schema:Person
228 sg:person.0727645116.99 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
229 schema:familyName Sutton
230 schema:givenName Sarah E
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727645116.99
232 rdf:type schema:Person
233 sg:pub.10.1038/316273a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000554641
234 https://doi.org/10.1038/316273a0
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/35047014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016895952
237 https://doi.org/10.1038/35047014
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/354311a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050462145
240 https://doi.org/10.1038/354311a0
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/4209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027218113
243 https://doi.org/10.1038/4209
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/ncb1526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017893678
246 https://doi.org/10.1038/ncb1526
247 rdf:type schema:CreativeWork
248 sg:pub.10.1038/nsb719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021135221
249 https://doi.org/10.1038/nsb719
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/nsb796 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008933904
252 https://doi.org/10.1038/nsb796
253 rdf:type schema:CreativeWork
254 https://app.dimensions.ai/details/publication/pub.1079343524 schema:CreativeWork
255 https://doi.org/10.1016/0076-6879(95)56003-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035685402
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/0076-6879(95)56011-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001885162
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1016/j.cell.2005.10.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041736927
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1016/j.chom.2008.08.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045443968
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1016/j.micinf.2007.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006014775
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1016/j.str.2004.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029440917
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/j.tcb.2006.11.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035540369
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/j.tibs.2005.02.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005801037
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/s0076-6879(97)76066-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004907170
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1016/s0092-8674(00)81442-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044053295
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1016/s0092-8674(04)00003-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013496682
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1034/j.1600-0854.2001.20907.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027630227
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1046/j.1365-2958.2002.02952.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033221175
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1046/j.1462-5822.2000.00082.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024476138
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1074/jbc.m410820200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038634308
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1074/jbc.m502509200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008711773
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1083/jcb.200705021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016209052
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1093/emboj/19.13.3235 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043175432
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1093/emboj/21.6.1315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045395479
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1093/emboj/cdf329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019829073
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1107/s0108767390010224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014210169
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1107/s0907444994003112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025573949
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1107/s0907444998003254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019779527
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1107/s0909049503023938 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003067022
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1111/j.1365-2958.1996.tb02497.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043021690
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1111/j.1365-2958.2006.05109.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042603055
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1111/j.1462-5822.2008.01136.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038278115
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1111/j.1462-5822.2008.01254.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006394094
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1126/science.1062023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033212920
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1126/science.1067025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024140312
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1126/science.1110225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012847668
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1146/annurev.cellbio.21.020604.150721 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042781454
318 rdf:type schema:CreativeWork
319 https://www.grid.ac/institutes/grid.12527.33 schema:alternateName Tsinghua University
320 schema:name Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
321 National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China.
322 rdf:type schema:Organization
323 https://www.grid.ac/institutes/grid.267313.2 schema:alternateName The University of Texas Southwestern Medical Center
324 schema:name Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
325 rdf:type schema:Organization
326 https://www.grid.ac/institutes/grid.410717.4 schema:alternateName National Institute of Biological Sciences, Beijing
327 schema:name National Institute of Biological Sciences, Zhong Guan Cun Life Science Park, Beijing, China.
328 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...