Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-10

AUTHORS

Byron DeLaBarre, Axel T Brunger

ABSTRACT

The ATPase p97/VCP affects multiple events within the cell. These events include the alteration of both nuclear and mitotic Golgi membranes, the dislocation of ubiquitylated proteins from the endoplasmic reticulum and regulation of the NF-kappa b pathway. Here we present the crystal structure of full-length Mus musculus p97/VCP in complex with a mixture of ADP and ADP-AlF(x) at a resolution of 4.7 A. This is the first complete hexameric structure of a protein containing tandem AAA (ATPases associated with a variety of cellular activities) domains. Comparison of the crystal structure and cryo-electron microscopy (EM) reconstructions reveals large conformational changes in the helical subdomains during the hydrolysis cycle. Structural and functional data imply a communication mechanism between the AAA domains. A Zn(2+) occludes the central pore of the hexamer, suggesting that substrate does not thread through the pore of the molecule. More... »

PAGES

856-863

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsb972

DOI

http://dx.doi.org/10.1038/nsb972

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051134048

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12949490


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