A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-08

AUTHORS

Sanjay C Panchal, Donald A Kaiser, Eduardo Torres, Thomas D Pollard, Michael K Rosen

ABSTRACT

Members of the Wiskott-Aldrich syndrome protein (WASP) family link Rho GTPase signaling pathways to the cytoskeleton through a multiprotein assembly called Arp2/3 complex. The C-terminal VCA regions (verprolin-homology, central hydrophobic, and acidic regions) of WASP and its relatives stimulate Arp2/3 complex to nucleate actin filament branches. Here we show by differential line broadening in NMR spectra that the C (central) and A (acidic) segments of VCA domains from WASP, N-WASP and Scar bind Arp2/3 complex. The C regions of these proteins have a conserved sequence motif consisting of hydrophobic residues and an arginine residue. Point mutations in this conserved sequence motif suggest that it forms an amphipathic helix that is required in biochemical assays for activation of Arp2/3 complex. Key residues in this motif are buried through contacts with the GTPase binding domain in the autoinhibited structure of WASP and N-WASP, indicating that sequestration of these residues is an important aspect of autoinhibition. More... »

PAGES

591-598

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsb952

DOI

http://dx.doi.org/10.1038/nsb952

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020429132

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12872157


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actin-Related Protein 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actin-Related Protein 3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoskeletal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macromolecular Substances", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multiprotein Complexes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Magnetic Resonance, Biomolecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Secondary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Wiskott-Aldrich Syndrome Protein", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Panchal", 
        "givenName": "Sanjay C", 
        "id": "sg:person.01214270236.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214270236.10"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kaiser", 
        "givenName": "Donald A", 
        "id": "sg:person.01241776514.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241776514.72"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Torres", 
        "givenName": "Eduardo", 
        "id": "sg:person.01306034726.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306034726.63"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Pollard", 
        "givenName": "Thomas D", 
        "id": "sg:person.01063276014.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063276014.20"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Rosen", 
        "givenName": "Michael K", 
        "id": "sg:person.0773034013.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773034013.13"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00404272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004332552", 
          "https://doi.org/10.1007/bf00404272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00404272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004332552", 
          "https://doi.org/10.1007/bf00404272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.150.6.1299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005546848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(02)00015-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007009842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(02)00015-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007009842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(99)00063-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010221520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00175245", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010505080", 
          "https://doi.org/10.1007/bf00175245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.7.3739", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011319079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80757-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012376331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.2000.2785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013027272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/criai:20:1:61", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013891811", 
          "https://doi.org/10.1385/criai:20:1:61"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)00015-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015776418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00603-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018290489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/85886", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019156611", 
          "https://doi.org/10.1038/85886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/85886", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019156611", 
          "https://doi.org/10.1038/85886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1011239023422", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019807161", 
          "https://doi.org/10.1023/a:1011239023422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.145.5.1009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021675451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.150.6.1311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027694658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m006407200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031681322"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.261419298", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034765065"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035769834", 
          "https://doi.org/10.1038/nature00859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035769834", 
          "https://doi.org/10.1038/nature00859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(80)91175-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036878803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1066333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038252329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v99.6.2268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038668755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c100476200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039466340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35050590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040671415", 
          "https://doi.org/10.1038/35050590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35050590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040671415", 
          "https://doi.org/10.1038/35050590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.190351397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045182003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35004513", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045390976", 
          "https://doi.org/10.1038/35004513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35004513", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045390976", 
          "https://doi.org/10.1038/35004513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(00)01871-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046591467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80732-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046826381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(99)80014-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047751326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1997.8064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048073733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.70.1.649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050161526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmrb.1994.1032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051131215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(01)00298-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051623985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00321a054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055170141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0157215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055194913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0157215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055194913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi991843+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055218451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi991843+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055218451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00105a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055707348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja991881g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055872371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1059975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062444572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1063025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062445284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.290.5492.801", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062571886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074688617", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00917.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082990333"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-08", 
    "datePublishedReg": "2003-08-01", 
    "description": "Members of the Wiskott-Aldrich syndrome protein (WASP) family link Rho GTPase signaling pathways to the cytoskeleton through a multiprotein assembly called Arp2/3 complex. The C-terminal VCA regions (verprolin-homology, central hydrophobic, and acidic regions) of WASP and its relatives stimulate Arp2/3 complex to nucleate actin filament branches. Here we show by differential line broadening in NMR spectra that the C (central) and A (acidic) segments of VCA domains from WASP, N-WASP and Scar bind Arp2/3 complex. The C regions of these proteins have a conserved sequence motif consisting of hydrophobic residues and an arginine residue. Point mutations in this conserved sequence motif suggest that it forms an amphipathic helix that is required in biochemical assays for activation of Arp2/3 complex. Key residues in this motif are buried through contacts with the GTPase binding domain in the autoinhibited structure of WASP and N-WASP, indicating that sequestration of these residues is an important aspect of autoinhibition.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nsb952", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1295033", 
        "issn": [
          "1545-9993", 
          "2331-365X"
        ], 
        "name": "Nature Structural & Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex", 
    "pagination": "591-598", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "61570e6e19008a0689a198199e6a7163d8f508c075726ed9e3c13f5b8a227038"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12872157"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421566"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nsb952"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1020429132"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nsb952", 
      "https://app.dimensions.ai/details/publication/pub.1020429132"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53999_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nsmb/journal/v10/n8/full/nsb952.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsb952'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsb952'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsb952'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsb952'


 

This table displays all metadata directly associated to this object as RDF triples.

298 TRIPLES      21 PREDICATES      89 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nsb952 schema:about N0539b894dca941df895178e0cf0c31e8
2 N0a637972001a4c1682f3ae12eec80b06
3 N115a1ae68fc4476799a4ba0cb0e3f19d
4 N2ec3651d74864a6f930a4aaaa0a04d7d
5 N334d4a846c114f3ba25e26f992ddfad7
6 N3601e1976e114cb8870f41632dfae704
7 N45d20042193149ee9dd1de45a713f1f2
8 N58a737d294644e89b33f63133316b6f0
9 N8172aa5b8c214e95aa1987572e4b1e4d
10 N8757b5d11fd7436385482c3a0a963936
11 N8bf5bc9c29ae444eae08c91849f074d4
12 N9c25cb7ec5ef443685ca31f9a76482ec
13 Na4b182e93ef7429d8a324670a1b51ec5
14 Nab1d8540481846c1b48b330b5e8df1ba
15 Nb19d3fb2d49b4cefb8af74ca0583d55c
16 Ncd40d27ea27045da8e43594cf4be4fae
17 Nd1886fa835264e138525561b5e9b686e
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author N9cb2b2490aa64a5795f19842accbb2bd
21 schema:citation sg:pub.10.1007/bf00175245
22 sg:pub.10.1007/bf00197809
23 sg:pub.10.1007/bf00404272
24 sg:pub.10.1023/a:1011239023422
25 sg:pub.10.1038/35004513
26 sg:pub.10.1038/35050590
27 sg:pub.10.1038/85886
28 sg:pub.10.1038/nature00859
29 sg:pub.10.1385/criai:20:1:61
30 https://app.dimensions.ai/details/publication/pub.1074688617
31 https://doi.org/10.1002/j.1460-2075.1996.tb00917.x
32 https://doi.org/10.1006/bbrc.1997.8064
33 https://doi.org/10.1006/bbrc.2000.2785
34 https://doi.org/10.1006/jmrb.1994.1032
35 https://doi.org/10.1016/0006-291x(80)91175-4
36 https://doi.org/10.1016/s0092-8674(00)80732-1
37 https://doi.org/10.1016/s0092-8674(00)80757-6
38 https://doi.org/10.1016/s0955-0674(01)00298-8
39 https://doi.org/10.1016/s0955-0674(02)00015-7
40 https://doi.org/10.1016/s0955-0674(99)00063-0
41 https://doi.org/10.1016/s0955-0674(99)80014-3
42 https://doi.org/10.1016/s0960-9822(01)00603-0
43 https://doi.org/10.1016/s0960-9822(98)00015-3
44 https://doi.org/10.1016/s0962-8924(00)01871-7
45 https://doi.org/10.1021/bi00321a054
46 https://doi.org/10.1021/bi0157215
47 https://doi.org/10.1021/bi991843+
48 https://doi.org/10.1021/ja00105a005
49 https://doi.org/10.1021/ja991881g
50 https://doi.org/10.1073/pnas.190351397
51 https://doi.org/10.1073/pnas.261419298
52 https://doi.org/10.1073/pnas.96.7.3739
53 https://doi.org/10.1074/jbc.c100476200
54 https://doi.org/10.1074/jbc.m006407200
55 https://doi.org/10.1083/jcb.145.5.1009
56 https://doi.org/10.1083/jcb.150.6.1299
57 https://doi.org/10.1083/jcb.150.6.1311
58 https://doi.org/10.1126/science.1059975
59 https://doi.org/10.1126/science.1063025
60 https://doi.org/10.1126/science.1066333
61 https://doi.org/10.1126/science.290.5492.801
62 https://doi.org/10.1146/annurev.biochem.70.1.649
63 https://doi.org/10.1182/blood.v99.6.2268
64 schema:datePublished 2003-08
65 schema:datePublishedReg 2003-08-01
66 schema:description Members of the Wiskott-Aldrich syndrome protein (WASP) family link Rho GTPase signaling pathways to the cytoskeleton through a multiprotein assembly called Arp2/3 complex. The C-terminal VCA regions (verprolin-homology, central hydrophobic, and acidic regions) of WASP and its relatives stimulate Arp2/3 complex to nucleate actin filament branches. Here we show by differential line broadening in NMR spectra that the C (central) and A (acidic) segments of VCA domains from WASP, N-WASP and Scar bind Arp2/3 complex. The C regions of these proteins have a conserved sequence motif consisting of hydrophobic residues and an arginine residue. Point mutations in this conserved sequence motif suggest that it forms an amphipathic helix that is required in biochemical assays for activation of Arp2/3 complex. Key residues in this motif are buried through contacts with the GTPase binding domain in the autoinhibited structure of WASP and N-WASP, indicating that sequestration of these residues is an important aspect of autoinhibition.
67 schema:genre research_article
68 schema:inLanguage en
69 schema:isAccessibleForFree false
70 schema:isPartOf N8c8f5b97ca4b4241bb0781ae6298b50a
71 Nbd621b7170f645f08ea6ac94d01baff6
72 sg:journal.1295033
73 schema:name A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex
74 schema:pagination 591-598
75 schema:productId N124e9acafb87433bb0312e51fa457e81
76 N20ad53e63da34148816f7799323829c9
77 N56153e22348f473c8ff2be9a3327d16c
78 N640479050c044549875298032a070692
79 N98076f24c2374c7cbea953843ef1105a
80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020429132
81 https://doi.org/10.1038/nsb952
82 schema:sdDatePublished 2019-04-11T12:13
83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
84 schema:sdPublisher N5536c19bb89642ac954d4e6442ff6bd9
85 schema:url http://www.nature.com/nsmb/journal/v10/n8/full/nsb952.html
86 sgo:license sg:explorer/license/
87 sgo:sdDataset articles
88 rdf:type schema:ScholarlyArticle
89 N0539b894dca941df895178e0cf0c31e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Proteins
91 rdf:type schema:DefinedTerm
92 N0a637972001a4c1682f3ae12eec80b06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Actin-Related Protein 2
94 rdf:type schema:DefinedTerm
95 N115a1ae68fc4476799a4ba0cb0e3f19d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Wiskott-Aldrich Syndrome Protein
97 rdf:type schema:DefinedTerm
98 N124e9acafb87433bb0312e51fa457e81 schema:name readcube_id
99 schema:value 61570e6e19008a0689a198199e6a7163d8f508c075726ed9e3c13f5b8a227038
100 rdf:type schema:PropertyValue
101 N20ad53e63da34148816f7799323829c9 schema:name dimensions_id
102 schema:value pub.1020429132
103 rdf:type schema:PropertyValue
104 N2ec3651d74864a6f930a4aaaa0a04d7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Actin-Related Protein 3
106 rdf:type schema:DefinedTerm
107 N334d4a846c114f3ba25e26f992ddfad7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Signal Transduction
109 rdf:type schema:DefinedTerm
110 N3601e1976e114cb8870f41632dfae704 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Sequence Homology, Amino Acid
112 rdf:type schema:DefinedTerm
113 N363cb14fff1640b6a7b9a949cd3d74dc rdf:first sg:person.01063276014.20
114 rdf:rest Ned002febf216424c934566a37f3616af
115 N3cd2429930dc4d5380f685e376fa9b4a rdf:first sg:person.01241776514.72
116 rdf:rest Nc1de0343d4274223afbbe01bb799f71e
117 N45d20042193149ee9dd1de45a713f1f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Protein Structure, Tertiary
119 rdf:type schema:DefinedTerm
120 N5536c19bb89642ac954d4e6442ff6bd9 schema:name Springer Nature - SN SciGraph project
121 rdf:type schema:Organization
122 N56153e22348f473c8ff2be9a3327d16c schema:name doi
123 schema:value 10.1038/nsb952
124 rdf:type schema:PropertyValue
125 N58a737d294644e89b33f63133316b6f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Binding Sites
127 rdf:type schema:DefinedTerm
128 N640479050c044549875298032a070692 schema:name pubmed_id
129 schema:value 12872157
130 rdf:type schema:PropertyValue
131 N8172aa5b8c214e95aa1987572e4b1e4d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Molecular Sequence Data
133 rdf:type schema:DefinedTerm
134 N8757b5d11fd7436385482c3a0a963936 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Nuclear Magnetic Resonance, Biomolecular
136 rdf:type schema:DefinedTerm
137 N8bf5bc9c29ae444eae08c91849f074d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Humans
139 rdf:type schema:DefinedTerm
140 N8c8f5b97ca4b4241bb0781ae6298b50a schema:volumeNumber 10
141 rdf:type schema:PublicationVolume
142 N98076f24c2374c7cbea953843ef1105a schema:name nlm_unique_id
143 schema:value 9421566
144 rdf:type schema:PropertyValue
145 N9c25cb7ec5ef443685ca31f9a76482ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Multiprotein Complexes
147 rdf:type schema:DefinedTerm
148 N9cb2b2490aa64a5795f19842accbb2bd rdf:first sg:person.01214270236.10
149 rdf:rest N3cd2429930dc4d5380f685e376fa9b4a
150 Na4b182e93ef7429d8a324670a1b51ec5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Models, Molecular
152 rdf:type schema:DefinedTerm
153 Nab1d8540481846c1b48b330b5e8df1ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Protein Structure, Secondary
155 rdf:type schema:DefinedTerm
156 Nb19d3fb2d49b4cefb8af74ca0583d55c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Macromolecular Substances
158 rdf:type schema:DefinedTerm
159 Nbd621b7170f645f08ea6ac94d01baff6 schema:issueNumber 8
160 rdf:type schema:PublicationIssue
161 Nc1de0343d4274223afbbe01bb799f71e rdf:first sg:person.01306034726.63
162 rdf:rest N363cb14fff1640b6a7b9a949cd3d74dc
163 Ncd40d27ea27045da8e43594cf4be4fae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Cytoskeletal Proteins
165 rdf:type schema:DefinedTerm
166 Nd1886fa835264e138525561b5e9b686e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Amino Acid Sequence
168 rdf:type schema:DefinedTerm
169 Ned002febf216424c934566a37f3616af rdf:first sg:person.0773034013.13
170 rdf:rest rdf:nil
171 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
172 schema:name Biological Sciences
173 rdf:type schema:DefinedTerm
174 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
175 schema:name Biochemistry and Cell Biology
176 rdf:type schema:DefinedTerm
177 sg:journal.1295033 schema:issn 1545-9993
178 2331-365X
179 schema:name Nature Structural & Molecular Biology
180 rdf:type schema:Periodical
181 sg:person.01063276014.20 schema:familyName Pollard
182 schema:givenName Thomas D
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063276014.20
184 rdf:type schema:Person
185 sg:person.01214270236.10 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
186 schema:familyName Panchal
187 schema:givenName Sanjay C
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214270236.10
189 rdf:type schema:Person
190 sg:person.01241776514.72 schema:familyName Kaiser
191 schema:givenName Donald A
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241776514.72
193 rdf:type schema:Person
194 sg:person.01306034726.63 schema:familyName Torres
195 schema:givenName Eduardo
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306034726.63
197 rdf:type schema:Person
198 sg:person.0773034013.13 schema:familyName Rosen
199 schema:givenName Michael K
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773034013.13
201 rdf:type schema:Person
202 sg:pub.10.1007/bf00175245 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010505080
203 https://doi.org/10.1007/bf00175245
204 rdf:type schema:CreativeWork
205 sg:pub.10.1007/bf00197809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001469691
206 https://doi.org/10.1007/bf00197809
207 rdf:type schema:CreativeWork
208 sg:pub.10.1007/bf00404272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004332552
209 https://doi.org/10.1007/bf00404272
210 rdf:type schema:CreativeWork
211 sg:pub.10.1023/a:1011239023422 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019807161
212 https://doi.org/10.1023/a:1011239023422
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/35004513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045390976
215 https://doi.org/10.1038/35004513
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/35050590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040671415
218 https://doi.org/10.1038/35050590
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/85886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019156611
221 https://doi.org/10.1038/85886
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/nature00859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035769834
224 https://doi.org/10.1038/nature00859
225 rdf:type schema:CreativeWork
226 sg:pub.10.1385/criai:20:1:61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013891811
227 https://doi.org/10.1385/criai:20:1:61
228 rdf:type schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1074688617 schema:CreativeWork
230 https://doi.org/10.1002/j.1460-2075.1996.tb00917.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082990333
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1006/bbrc.1997.8064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048073733
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1006/bbrc.2000.2785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013027272
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1006/jmrb.1994.1032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051131215
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0006-291x(80)91175-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036878803
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0092-8674(00)80732-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046826381
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0092-8674(00)80757-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012376331
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0955-0674(01)00298-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051623985
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0955-0674(02)00015-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007009842
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s0955-0674(99)00063-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010221520
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0955-0674(99)80014-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047751326
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0960-9822(01)00603-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018290489
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0960-9822(98)00015-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015776418
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/s0962-8924(00)01871-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046591467
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/bi00321a054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055170141
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1021/bi0157215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055194913
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1021/bi991843+ schema:sameAs https://app.dimensions.ai/details/publication/pub.1055218451
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1021/ja00105a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055707348
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1021/ja991881g schema:sameAs https://app.dimensions.ai/details/publication/pub.1055872371
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1073/pnas.190351397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045182003
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1073/pnas.261419298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034765065
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1073/pnas.96.7.3739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011319079
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1074/jbc.c100476200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039466340
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1074/jbc.m006407200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031681322
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1083/jcb.145.5.1009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021675451
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1083/jcb.150.6.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005546848
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1083/jcb.150.6.1311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027694658
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1126/science.1059975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062444572
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1126/science.1063025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445284
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1126/science.1066333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038252329
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1126/science.290.5492.801 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062571886
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1146/annurev.biochem.70.1.649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050161526
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1182/blood.v99.6.2268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038668755
295 rdf:type schema:CreativeWork
296 https://www.grid.ac/institutes/grid.267313.2 schema:alternateName The University of Texas Southwestern Medical Center
297 schema:name Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA.
298 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...