Conformational changes of the multifunction p97 AAA ATPase during its ATPase cycle View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-12

AUTHORS

Isabelle Rouiller, Byron DeLaBarre, Andrew P. May, William I. Weis, Axel T. Brunger, Ronald A. Milligan, Elizabeth M. Wilson-Kubalek

ABSTRACT

p97 (also called VCP), a member of the AAA ATPase family, is involved in several cellular processes, including membrane fusion and extraction of proteins from the endoplasmic reticulum for cytoplasmic degradation. We have studied the conformational changes that p97 undergoes during the ATPase cycle by cryo-EM and single-particle analysis. Three-dimensional maps show that the two AAA domains, D1 and D2, as well as the N-domains, experience conformational changes during ATP binding, ATP hydrolysis, P(i) release and ADP release. The N-domain is flexible in most nucleotide states except after ATP hydrolysis. The rings formed by D1 and D2 rotate with respect to each other, and the size of their axial openings fluctuates. Taken together, our results depict the movements that this and possibly other AAA ATPases can undergo during an ATPase cycle. More... »

PAGES

950-957

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nsb872

DOI

http://dx.doi.org/10.1038/nsb872

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035318555

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12434150


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JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nsb872'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nsb872'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nsb872'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nsb872'


 

This table displays all metadata directly associated to this object as RDF triples.

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