Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-06

AUTHORS

Mario R Capecchi

ABSTRACT

Gene targeting in mouse embryonic stem cells has become the 'gold standard' for determining gene function in mammals. Since its inception, this technology has revolutionized the study of mammalian biology and human medicine. Here I provide a personal account of the work that led to the generation of gene targeting which now lies at the centre of functional genomic analysis. More... »

PAGES

507-512

References to SciGraph publications

  • 2001-10. Generating mice with targeted mutations in NATURE MEDICINE
  • 1985-09. Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination in NATURE
  • 1981-07. Establishment in culture of pluripotential cells from mouse embryos in NATURE
  • 1981-11. Introduction of a rabbit β-globin gene into the mouse germ line in NATURE
  • 1995-06. Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11 in NATURE
  • 1989-11. Germ-line transmission of a disrupted β2microglobulin gene produced by homologous recombination in embryonic stem cells in NATURE
  • 2000-02. Maintenance of functional equivalence during paralogous Hox gene evolution in NATURE
  • 1990-08. Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development in NATURE
  • 2000-12. Fgf8 is required for outgrowth and patterning of the limbs in NATURE GENETICS
  • 1984-05. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines in NATURE
  • 1986-01. Analysis of homologous recombination in cultured mammalian cells in transient expression and stable transformation assays in SOMATIC CELL AND MOLECULAR GENETICS
  • 1989-03. Production of a mutation in mouse En-2 gene by homologous recombination in embryonic stem cells in NATURE
  • 1990-05. A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting in NATURE
  • 1982-02. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus in NATURE
  • 1988-11. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes in NATURE
  • 2004-09. The Knockout Mouse Project in NATURE GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nrg1619

    DOI

    http://dx.doi.org/10.1038/nrg1619

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15931173


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