Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-05

AUTHORS

Shengdar Q. Tsai, J. Keith Joung

ABSTRACT

CRISPR-Cas9 RNA-guided nucleases are a transformative technology for biology, genetics and medicine owing to the simplicity with which they can be programmed to cleave specific DNA target sites in living cells and organisms. However, to translate these powerful molecular tools into safe, effective clinical applications, it is of crucial importance to carefully define and improve their genome-wide specificities. Here, we outline our state-of-the-art understanding of target DNA recognition and cleavage by CRISPR-Cas9 nucleases, methods to determine and improve their specificities, and key considerations for how to evaluate and reduce off-target effects for research and therapeutic applications. More... »

PAGES

300-312

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    http://scigraph.springernature.com/pub.10.1038/nrg.2016.28

    DOI

    http://dx.doi.org/10.1038/nrg.2016.28

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010657174

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27087594


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