Large-area molecular patterning with polymer pen lithography View Full Text


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Article Info

DATE

2013-12

AUTHORS

Daniel J Eichelsdoerfer, Xing Liao, Maria D Cabezas, William Morris, Boya Radha, Keith A Brown, Louise R Giam, Adam B Braunschweig, Chad A Mirkin

ABSTRACT

The challenge of constructing surfaces with nanostructured chemical functionality is central to many areas of biology and biotechnology. This protocol describes the steps required for performing molecular printing using polymer pen lithography (PPL), a cantilever-free scanning probe-based technique that can generate sub-100-nm molecular features in a massively parallel fashion. To illustrate how such molecular printing can be used for a variety of biologically relevant applications, we detail the fabrication of the lithographic apparatus and the deposition of two materials, an alkanethiol and a polymer onto a gold and silicon surface, respectively, and show how the present approach can be used to generate nanostructures composed of proteins and metals. Finally, we describe how PPL enables researchers to easily create combinatorial arrays of nanostructures, a powerful approach for high-throughput screening. A typical protocol for fabricating PPL arrays and printing with the arrays takes 48-72 h to complete, including two overnight waiting steps. More... »

PAGES

2548-2560

References to SciGraph publications

  • 2010-09. Beam pen lithography in NATURE NANOTECHNOLOGY
  • 2007-10. High-resolution electrohydrodynamic jetĀ printing in NATURE MATERIALS
  • 2010-03. Soft lithography for micro- and nanoscale patterning in NATURE PROTOCOLS
  • 2009-08. Molecular printing in NATURE CHEMISTRY
  • 2011-01-27. Hard-tip, soft-spring lithography in NATURE
  • 2007-03. Applications of dip-pen nanolithography in NATURE NANOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2013.159

    DOI

    http://dx.doi.org/10.1038/nprot.2013.159

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015092497

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24263094


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