Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-04

AUTHORS

Marie-Theres Gansauge, Matthias Meyer

ABSTRACT

This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with ∼50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d. More... »

PAGES

737

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2013.038

DOI

http://dx.doi.org/10.1038/nprot.2013.038

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034246196

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23493070


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotinylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Single-Stranded", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "High-Throughput Nucleotide Sequencing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Evolutionary Anthropology", 
          "id": "https://www.grid.ac/institutes/grid.419518.0", 
          "name": [
            "Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gansauge", 
        "givenName": "Marie-Theres", 
        "id": "sg:person.0611745754.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611745754.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Evolutionary Anthropology", 
          "id": "https://www.grid.ac/institutes/grid.419518.0", 
          "name": [
            "Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meyer", 
        "givenName": "Matthias", 
        "id": "sg:person.01303122730.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303122730.44"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1126/science.1188021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002597304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002597304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07446", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003406391", 
          "https://doi.org/10.1038/nature07446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007480955", 
          "https://doi.org/10.1038/nature10242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1224344", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013028677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.02.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014092720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0000537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014829007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.091868.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017676111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018669887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018669887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020995997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020995997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021776500", 
          "https://doi.org/10.1038/nature08835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021776500", 
          "https://doi.org/10.1038/nature08835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkr771", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026092281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2009.11.068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028054623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0014004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031804493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2005.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032078389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2010-11-12-r119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033784968", 
          "https://doi.org/10.1186/gb-2010-11-12-r119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0034131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036440628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkp1163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036956881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038266369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.2004.2813", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038798539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0704665104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045401726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-12-382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048431087", 
          "https://doi.org/10.1186/1471-2164-12-382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050205294", 
          "https://doi.org/10.1038/nature09710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050205294", 
          "https://doi.org/10.1038/nature09710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.22.4742", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052479221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052925719", 
          "https://doi.org/10.1038/nature07517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm588", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053124372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0013042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053529462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep00074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053587913", 
          "https://doi.org/10.1038/srep00074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/pdb.prot5448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060411299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1174462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062460190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000113809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069096399"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-04", 
    "datePublishedReg": "2013-04-01", 
    "description": "This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with \u223c50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nprot.2013.038", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1037502", 
        "issn": [
          "1754-2189", 
          "1750-2799"
        ], 
        "name": "Nature Protocols", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA", 
    "pagination": "737", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a322b1936e619f4b377e47441f1f3e6c2a9f7c21e3bfe2e2e6a6c6459ed18f55"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23493070"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101284307"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nprot.2013.038"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034246196"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nprot.2013.038", 
      "https://app.dimensions.ai/details/publication/pub.1034246196"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000537.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nprot.2013.038"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'


 

This table displays all metadata directly associated to this object as RDF triples.

209 TRIPLES      21 PREDICATES      68 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nprot.2013.038 schema:about N167655341fd64ea080cf76665d49acd7
2 N368a2279b48b4cd988763b90cced3fc4
3 N3e4c35ae7249477687905691fa2da927
4 Nd758a65f51134521b35b8e2938bd38fb
5 Nd8aa2f1661f84114af4fadc0d65b0865
6 Ne80244b3d4d644898bd9e13c990cc316
7 Ned0f07b0ec024e899dd67ce14858f936
8 anzsrc-for:06
9 anzsrc-for:0604
10 schema:author Nae92f215ccc1468eade8d60673f32c16
11 schema:citation sg:pub.10.1038/nature03959
12 sg:pub.10.1038/nature07446
13 sg:pub.10.1038/nature07517
14 sg:pub.10.1038/nature08835
15 sg:pub.10.1038/nature09710
16 sg:pub.10.1038/nature10242
17 sg:pub.10.1038/srep00074
18 sg:pub.10.1186/1471-2164-12-382
19 sg:pub.10.1186/gb-2010-11-12-r119
20 https://doi.org/10.1016/j.ab.2005.11.002
21 https://doi.org/10.1016/j.cell.2012.02.028
22 https://doi.org/10.1016/j.cub.2009.11.068
23 https://doi.org/10.1073/pnas.0704665104
24 https://doi.org/10.1093/bioinformatics/btp324
25 https://doi.org/10.1093/nar/23.22.4742
26 https://doi.org/10.1093/nar/gkm588
27 https://doi.org/10.1093/nar/gkp1163
28 https://doi.org/10.1093/nar/gkr771
29 https://doi.org/10.1098/rspb.2004.2813
30 https://doi.org/10.1101/gr.091868.109
31 https://doi.org/10.1101/pdb.prot5448
32 https://doi.org/10.1126/science.1174462
33 https://doi.org/10.1126/science.1188021
34 https://doi.org/10.1126/science.1188046
35 https://doi.org/10.1126/science.1224344
36 https://doi.org/10.1177/002215540205000802
37 https://doi.org/10.1371/journal.pone.0000537
38 https://doi.org/10.1371/journal.pone.0013042
39 https://doi.org/10.1371/journal.pone.0014004
40 https://doi.org/10.1371/journal.pone.0034131
41 https://doi.org/10.2144/000112383
42 https://doi.org/10.2144/000113809
43 schema:datePublished 2013-04
44 schema:datePublishedReg 2013-04-01
45 schema:description This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with ∼50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree false
49 schema:isPartOf N705ed567dd524afe822911138c9cbbd0
50 Nb7db49c4d1cd4eebb4afa690f3b67620
51 sg:journal.1037502
52 schema:name Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
53 schema:pagination 737
54 schema:productId N84774d20dfb3419195e3988e90b4d97a
55 Nb1a272d27dc6428f98ec04dd69b1649a
56 Nbe8e33445c0a49298b421925c76eaf3a
57 Nd18d987e1e364e57a17ce56141439e4a
58 Ned4064f4f3974569b050508d357fafdc
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034246196
60 https://doi.org/10.1038/nprot.2013.038
61 schema:sdDatePublished 2019-04-10T20:51
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher Nb3c2638a6b1c4f628acd417233631263
64 schema:url https://www.nature.com/articles/nprot.2013.038
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N167655341fd64ea080cf76665d49acd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name DNA, Single-Stranded
70 rdf:type schema:DefinedTerm
71 N368a2279b48b4cd988763b90cced3fc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name High-Throughput Nucleotide Sequencing
73 rdf:type schema:DefinedTerm
74 N3e4c35ae7249477687905691fa2da927 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Sequence Analysis, DNA
76 rdf:type schema:DefinedTerm
77 N705ed567dd524afe822911138c9cbbd0 schema:issueNumber 4
78 rdf:type schema:PublicationIssue
79 N84774d20dfb3419195e3988e90b4d97a schema:name dimensions_id
80 schema:value pub.1034246196
81 rdf:type schema:PropertyValue
82 Nae92f215ccc1468eade8d60673f32c16 rdf:first sg:person.0611745754.99
83 rdf:rest Nd148659ce7d34904bf09ecad7648baa8
84 Nb1a272d27dc6428f98ec04dd69b1649a schema:name doi
85 schema:value 10.1038/nprot.2013.038
86 rdf:type schema:PropertyValue
87 Nb3c2638a6b1c4f628acd417233631263 schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 Nb7db49c4d1cd4eebb4afa690f3b67620 schema:volumeNumber 8
90 rdf:type schema:PublicationVolume
91 Nbe8e33445c0a49298b421925c76eaf3a schema:name nlm_unique_id
92 schema:value 101284307
93 rdf:type schema:PropertyValue
94 Nd148659ce7d34904bf09ecad7648baa8 rdf:first sg:person.01303122730.44
95 rdf:rest rdf:nil
96 Nd18d987e1e364e57a17ce56141439e4a schema:name pubmed_id
97 schema:value 23493070
98 rdf:type schema:PropertyValue
99 Nd758a65f51134521b35b8e2938bd38fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name DNA Primers
101 rdf:type schema:DefinedTerm
102 Nd8aa2f1661f84114af4fadc0d65b0865 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Gene Library
104 rdf:type schema:DefinedTerm
105 Ne80244b3d4d644898bd9e13c990cc316 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Biotinylation
107 rdf:type schema:DefinedTerm
108 Ned0f07b0ec024e899dd67ce14858f936 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Polymerase Chain Reaction
110 rdf:type schema:DefinedTerm
111 Ned4064f4f3974569b050508d357fafdc schema:name readcube_id
112 schema:value a322b1936e619f4b377e47441f1f3e6c2a9f7c21e3bfe2e2e6a6c6459ed18f55
113 rdf:type schema:PropertyValue
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 sg:journal.1037502 schema:issn 1750-2799
121 1754-2189
122 schema:name Nature Protocols
123 rdf:type schema:Periodical
124 sg:person.01303122730.44 schema:affiliation https://www.grid.ac/institutes/grid.419518.0
125 schema:familyName Meyer
126 schema:givenName Matthias
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303122730.44
128 rdf:type schema:Person
129 sg:person.0611745754.99 schema:affiliation https://www.grid.ac/institutes/grid.419518.0
130 schema:familyName Gansauge
131 schema:givenName Marie-Theres
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611745754.99
133 rdf:type schema:Person
134 sg:pub.10.1038/nature03959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
135 https://doi.org/10.1038/nature03959
136 rdf:type schema:CreativeWork
137 sg:pub.10.1038/nature07446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003406391
138 https://doi.org/10.1038/nature07446
139 rdf:type schema:CreativeWork
140 sg:pub.10.1038/nature07517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052925719
141 https://doi.org/10.1038/nature07517
142 rdf:type schema:CreativeWork
143 sg:pub.10.1038/nature08835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021776500
144 https://doi.org/10.1038/nature08835
145 rdf:type schema:CreativeWork
146 sg:pub.10.1038/nature09710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050205294
147 https://doi.org/10.1038/nature09710
148 rdf:type schema:CreativeWork
149 sg:pub.10.1038/nature10242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007480955
150 https://doi.org/10.1038/nature10242
151 rdf:type schema:CreativeWork
152 sg:pub.10.1038/srep00074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053587913
153 https://doi.org/10.1038/srep00074
154 rdf:type schema:CreativeWork
155 sg:pub.10.1186/1471-2164-12-382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048431087
156 https://doi.org/10.1186/1471-2164-12-382
157 rdf:type schema:CreativeWork
158 sg:pub.10.1186/gb-2010-11-12-r119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033784968
159 https://doi.org/10.1186/gb-2010-11-12-r119
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.ab.2005.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032078389
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.cell.2012.02.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014092720
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/j.cub.2009.11.068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028054623
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1073/pnas.0704665104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045401726
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1093/bioinformatics/btp324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038266369
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1093/nar/23.22.4742 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052479221
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1093/nar/gkm588 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053124372
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1093/nar/gkp1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036956881
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1093/nar/gkr771 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026092281
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1098/rspb.2004.2813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038798539
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1101/gr.091868.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017676111
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1101/pdb.prot5448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060411299
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1126/science.1174462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062460190
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1126/science.1188021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002597304
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1126/science.1188046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020995997
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1126/science.1224344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013028677
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1177/002215540205000802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018669887
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1371/journal.pone.0000537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014829007
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1371/journal.pone.0013042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053529462
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1371/journal.pone.0014004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031804493
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1371/journal.pone.0034131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036440628
202 rdf:type schema:CreativeWork
203 https://doi.org/10.2144/000112383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095463
204 rdf:type schema:CreativeWork
205 https://doi.org/10.2144/000113809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069096399
206 rdf:type schema:CreativeWork
207 https://www.grid.ac/institutes/grid.419518.0 schema:alternateName Max Planck Institute for Evolutionary Anthropology
208 schema:name Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
209 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...