Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-04

AUTHORS

Marie-Theres Gansauge, Matthias Meyer

ABSTRACT

This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with ∼50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d. More... »

PAGES

737

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2013.038

DOI

http://dx.doi.org/10.1038/nprot.2013.038

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034246196

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23493070


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotinylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Single-Stranded", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "High-Throughput Nucleotide Sequencing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Evolutionary Anthropology", 
          "id": "https://www.grid.ac/institutes/grid.419518.0", 
          "name": [
            "Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gansauge", 
        "givenName": "Marie-Theres", 
        "id": "sg:person.0611745754.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611745754.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Evolutionary Anthropology", 
          "id": "https://www.grid.ac/institutes/grid.419518.0", 
          "name": [
            "Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meyer", 
        "givenName": "Matthias", 
        "id": "sg:person.01303122730.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303122730.44"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1126/science.1188021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002597304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002597304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07446", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003406391", 
          "https://doi.org/10.1038/nature07446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007480955", 
          "https://doi.org/10.1038/nature10242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1224344", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013028677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.02.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014092720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0000537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014829007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.091868.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017676111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018669887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018669887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020995997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1188046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020995997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021776500", 
          "https://doi.org/10.1038/nature08835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021776500", 
          "https://doi.org/10.1038/nature08835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkr771", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026092281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2009.11.068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028054623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0014004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031804493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2005.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032078389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2010-11-12-r119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033784968", 
          "https://doi.org/10.1186/gb-2010-11-12-r119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0034131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036440628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkp1163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036956881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038266369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.2004.2813", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038798539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0704665104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045401726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-12-382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048431087", 
          "https://doi.org/10.1186/1471-2164-12-382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050205294", 
          "https://doi.org/10.1038/nature09710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050205294", 
          "https://doi.org/10.1038/nature09710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.22.4742", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052479221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052925719", 
          "https://doi.org/10.1038/nature07517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm588", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053124372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0013042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053529462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep00074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053587913", 
          "https://doi.org/10.1038/srep00074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/pdb.prot5448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060411299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1174462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062460190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000113809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069096399"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-04", 
    "datePublishedReg": "2013-04-01", 
    "description": "This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with \u223c50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nprot.2013.038", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1037502", 
        "issn": [
          "1754-2189", 
          "1750-2799"
        ], 
        "name": "Nature Protocols", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA", 
    "pagination": "737", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a322b1936e619f4b377e47441f1f3e6c2a9f7c21e3bfe2e2e6a6c6459ed18f55"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23493070"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101284307"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nprot.2013.038"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034246196"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nprot.2013.038", 
      "https://app.dimensions.ai/details/publication/pub.1034246196"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000537.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nprot.2013.038"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2013.038'


 

This table displays all metadata directly associated to this object as RDF triples.

209 TRIPLES      21 PREDICATES      68 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nprot.2013.038 schema:about N05ae6c7b802a4b25a160974af0f9d052
2 N7e670eea86334035a15460536e3f7dcc
3 N8f94c8c8b61b4073a21796dff389531b
4 Na8b56162d4de40c184af3d4a804094eb
5 Naf0e83f5c1bd430da6e00994ded7fc15
6 Nb92dc12713544e59b15ad472aa18822c
7 Nee01b8c52aba420a996db6d0d888ff5b
8 anzsrc-for:06
9 anzsrc-for:0604
10 schema:author N05fe6cb08b8c41e4a329d6291f9d0aa5
11 schema:citation sg:pub.10.1038/nature03959
12 sg:pub.10.1038/nature07446
13 sg:pub.10.1038/nature07517
14 sg:pub.10.1038/nature08835
15 sg:pub.10.1038/nature09710
16 sg:pub.10.1038/nature10242
17 sg:pub.10.1038/srep00074
18 sg:pub.10.1186/1471-2164-12-382
19 sg:pub.10.1186/gb-2010-11-12-r119
20 https://doi.org/10.1016/j.ab.2005.11.002
21 https://doi.org/10.1016/j.cell.2012.02.028
22 https://doi.org/10.1016/j.cub.2009.11.068
23 https://doi.org/10.1073/pnas.0704665104
24 https://doi.org/10.1093/bioinformatics/btp324
25 https://doi.org/10.1093/nar/23.22.4742
26 https://doi.org/10.1093/nar/gkm588
27 https://doi.org/10.1093/nar/gkp1163
28 https://doi.org/10.1093/nar/gkr771
29 https://doi.org/10.1098/rspb.2004.2813
30 https://doi.org/10.1101/gr.091868.109
31 https://doi.org/10.1101/pdb.prot5448
32 https://doi.org/10.1126/science.1174462
33 https://doi.org/10.1126/science.1188021
34 https://doi.org/10.1126/science.1188046
35 https://doi.org/10.1126/science.1224344
36 https://doi.org/10.1177/002215540205000802
37 https://doi.org/10.1371/journal.pone.0000537
38 https://doi.org/10.1371/journal.pone.0013042
39 https://doi.org/10.1371/journal.pone.0014004
40 https://doi.org/10.1371/journal.pone.0034131
41 https://doi.org/10.2144/000112383
42 https://doi.org/10.2144/000113809
43 schema:datePublished 2013-04
44 schema:datePublishedReg 2013-04-01
45 schema:description This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of short or degraded DNA from other sources, and it can also be used for sequencing oligonucleotides. Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase. A second adapter is attached by blunt-end ligation, and library preparation is completed by PCR amplification. We estimate that intact DNA strands are recovered in the library with ∼50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree false
49 schema:isPartOf N4004e9afbdd4403baf1b7900be0272ba
50 Nb1006275dd8d4e13950c453d9823d387
51 sg:journal.1037502
52 schema:name Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
53 schema:pagination 737
54 schema:productId N009d7cb2d3274f47b6fa482205c13a0d
55 N2ffa3be049124a43a816e97e7e6f3aac
56 N865e2ead454c4884ad1af8234d73d957
57 Nb1a822a0ff5b4a708d0892d8d3d97828
58 Ne4fa278f7c4e4a029ab9eb523df4c3f5
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034246196
60 https://doi.org/10.1038/nprot.2013.038
61 schema:sdDatePublished 2019-04-10T20:51
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher Nd2ca0a77bc4a4acb87c9d60215999c7a
64 schema:url https://www.nature.com/articles/nprot.2013.038
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N009d7cb2d3274f47b6fa482205c13a0d schema:name doi
69 schema:value 10.1038/nprot.2013.038
70 rdf:type schema:PropertyValue
71 N05ae6c7b802a4b25a160974af0f9d052 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name High-Throughput Nucleotide Sequencing
73 rdf:type schema:DefinedTerm
74 N05fe6cb08b8c41e4a329d6291f9d0aa5 rdf:first sg:person.0611745754.99
75 rdf:rest Nfa2f6f4dc79741efa895df0026a5e68c
76 N2ffa3be049124a43a816e97e7e6f3aac schema:name nlm_unique_id
77 schema:value 101284307
78 rdf:type schema:PropertyValue
79 N4004e9afbdd4403baf1b7900be0272ba schema:volumeNumber 8
80 rdf:type schema:PublicationVolume
81 N7e670eea86334035a15460536e3f7dcc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Gene Library
83 rdf:type schema:DefinedTerm
84 N865e2ead454c4884ad1af8234d73d957 schema:name pubmed_id
85 schema:value 23493070
86 rdf:type schema:PropertyValue
87 N8f94c8c8b61b4073a21796dff389531b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Sequence Analysis, DNA
89 rdf:type schema:DefinedTerm
90 Na8b56162d4de40c184af3d4a804094eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Polymerase Chain Reaction
92 rdf:type schema:DefinedTerm
93 Naf0e83f5c1bd430da6e00994ded7fc15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Biotinylation
95 rdf:type schema:DefinedTerm
96 Nb1006275dd8d4e13950c453d9823d387 schema:issueNumber 4
97 rdf:type schema:PublicationIssue
98 Nb1a822a0ff5b4a708d0892d8d3d97828 schema:name dimensions_id
99 schema:value pub.1034246196
100 rdf:type schema:PropertyValue
101 Nb92dc12713544e59b15ad472aa18822c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name DNA, Single-Stranded
103 rdf:type schema:DefinedTerm
104 Nd2ca0a77bc4a4acb87c9d60215999c7a schema:name Springer Nature - SN SciGraph project
105 rdf:type schema:Organization
106 Ne4fa278f7c4e4a029ab9eb523df4c3f5 schema:name readcube_id
107 schema:value a322b1936e619f4b377e47441f1f3e6c2a9f7c21e3bfe2e2e6a6c6459ed18f55
108 rdf:type schema:PropertyValue
109 Nee01b8c52aba420a996db6d0d888ff5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name DNA Primers
111 rdf:type schema:DefinedTerm
112 Nfa2f6f4dc79741efa895df0026a5e68c rdf:first sg:person.01303122730.44
113 rdf:rest rdf:nil
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 sg:journal.1037502 schema:issn 1750-2799
121 1754-2189
122 schema:name Nature Protocols
123 rdf:type schema:Periodical
124 sg:person.01303122730.44 schema:affiliation https://www.grid.ac/institutes/grid.419518.0
125 schema:familyName Meyer
126 schema:givenName Matthias
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303122730.44
128 rdf:type schema:Person
129 sg:person.0611745754.99 schema:affiliation https://www.grid.ac/institutes/grid.419518.0
130 schema:familyName Gansauge
131 schema:givenName Marie-Theres
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611745754.99
133 rdf:type schema:Person
134 sg:pub.10.1038/nature03959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
135 https://doi.org/10.1038/nature03959
136 rdf:type schema:CreativeWork
137 sg:pub.10.1038/nature07446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003406391
138 https://doi.org/10.1038/nature07446
139 rdf:type schema:CreativeWork
140 sg:pub.10.1038/nature07517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052925719
141 https://doi.org/10.1038/nature07517
142 rdf:type schema:CreativeWork
143 sg:pub.10.1038/nature08835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021776500
144 https://doi.org/10.1038/nature08835
145 rdf:type schema:CreativeWork
146 sg:pub.10.1038/nature09710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050205294
147 https://doi.org/10.1038/nature09710
148 rdf:type schema:CreativeWork
149 sg:pub.10.1038/nature10242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007480955
150 https://doi.org/10.1038/nature10242
151 rdf:type schema:CreativeWork
152 sg:pub.10.1038/srep00074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053587913
153 https://doi.org/10.1038/srep00074
154 rdf:type schema:CreativeWork
155 sg:pub.10.1186/1471-2164-12-382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048431087
156 https://doi.org/10.1186/1471-2164-12-382
157 rdf:type schema:CreativeWork
158 sg:pub.10.1186/gb-2010-11-12-r119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033784968
159 https://doi.org/10.1186/gb-2010-11-12-r119
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.ab.2005.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032078389
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.cell.2012.02.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014092720
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/j.cub.2009.11.068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028054623
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1073/pnas.0704665104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045401726
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1093/bioinformatics/btp324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038266369
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1093/nar/23.22.4742 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052479221
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1093/nar/gkm588 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053124372
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1093/nar/gkp1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036956881
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1093/nar/gkr771 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026092281
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1098/rspb.2004.2813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038798539
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1101/gr.091868.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017676111
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1101/pdb.prot5448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060411299
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1126/science.1174462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062460190
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1126/science.1188021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002597304
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1126/science.1188046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020995997
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1126/science.1224344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013028677
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1177/002215540205000802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018669887
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1371/journal.pone.0000537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014829007
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1371/journal.pone.0013042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053529462
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1371/journal.pone.0014004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031804493
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1371/journal.pone.0034131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036440628
202 rdf:type schema:CreativeWork
203 https://doi.org/10.2144/000112383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095463
204 rdf:type schema:CreativeWork
205 https://doi.org/10.2144/000113809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069096399
206 rdf:type schema:CreativeWork
207 https://www.grid.ac/institutes/grid.419518.0 schema:alternateName Max Planck Institute for Evolutionary Anthropology
208 schema:name Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
209 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...