Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-03-14

AUTHORS

Lingling Neng, Wenjing Zhang, Ahmed Hassan, Marcin Zemla, Allan Kachelmeier, Anders Fridberger, Manfred Auer, Xiaorui Shi

ABSTRACT

This protocol describes a growth medium–based approach for obtaining cochlear endothelial cells (ECs), pericytes (PCs) and perivascular resident macrophage-like melanocytes (PVM/Ms) from the stria vascularis of mice aged between P10 and P15 (P, postnatal day). The procedure does not involve mechanical or enzymatic digestion of the sample tissue. Explants of stria vascularis, 'mini-chips', are selectively cultured in growth medium, and primary cell lines are obtained in 7–10 d. The method is simple and reliable, and it provides high-quality ECs, PVM/Ms and PCs with a purity >90% after two passages. This protocol is suitable for producing primary culture cells from organs and tissues of small volume and high anatomical complexity, such as the inner ear capillaries. The highly purified primary cell lines enable cell culture–based in vitro modeling of cell-cell interactions, barrier control function and drug action. More... »

PAGES

709-720

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2013.033

DOI

http://dx.doi.org/10.1038/nprot.2013.033

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031991236

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23493068


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