Exosome-mediated delivery of siRNA in vitro and in vivo View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-11-15

AUTHORS

Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, Chris Gardiner, Lydia Alvarez-Erviti, Ian L Sargent, Matthew J A Wood

ABSTRACT

The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused with a peptide ligand. Next, we explain how to purify and characterize exosomes from transfected cell supernatant. Next, we detail crucial steps for loading siRNA into exosomes. Finally, we outline how to use exosomes to efficiently deliver siRNA in vitro and in vivo in mouse brain. Examples of anticipated results in which exosome-mediated siRNA delivery is evaluated by functional assays and imaging are also provided. The entire protocol takes ∼3 weeks. More... »

PAGES

2112-2126

Journal

TITLE

Nature Protocols

ISSUE

12

VOLUME

7

Related Patents

  • Analysis Of Genomic Dna, Rna, And Proteins In Exosomes For Diagnosis And Theranosis
  • Type Vii Crispr Proteins And Systems
  • Method Of Detecting The Presence Or Absence Of Autoantibodies
  • Crispr Having Or Associated With Destabilization Domains
  • Novel Cas12b Enzymes And Systems
  • Compositions And Methods Of Use Of Crispr-Cas Systems In Nucleotide Repeat Disorders
  • Delivery, Engineering And Optimization Of Systems, Methods And Compositions For Sequence Manipulation And Therapeutic Applications
  • Novel Crispr Enzymes And Systems
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Genome Editing
  • Use Of Exosomes For The Treatment Of Disease
  • Methods And Compositions For Optochemical Control Of Crispr-Cas9
  • Crispr-Cas Systems Having Destabilization Domain
  • Cell-Type Specific Exosomes And Use Thereof
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Targeting Disorders And Diseases Using Viral Components
  • Systems, Methods, And Compositions For Targeted Nucleic Acid Editing
  • Novel Crispr Enzymes And Systems
  • Therapeutic Delivery Vesicles
  • Novel Nucleic Acid Modifiers
  • Cpf1 Complexes With Reduced Indel Activity
  • Novel Delivery Of Large Payloads
  • Modulation Of Novel Immune Checkpoint Targets
  • Genome Editing Using Cas9 Nickases
  • Therapeutic Delivery Vesicles
  • Nuclear-Derived Exosomes And Methods Of Use Thereof
  • Protected Guide Rnas (Pgrnas)
  • Cell Sorting
  • Oligonucleotide Compounds For Targeting Huntingtin Mrna
  • Esosomi Ingegnerizzati Per Il Trattamento Della Leucemia Mieloide Cronica
  • Therapeutic Delivery Vesicles
  • Delivery, Engineering And Optimization Of Systems, Methods And Compositions For Sequence Manipulation And Therapeutic Applications
  • Novel Crispr Enzymes And Systems
  • Novel Crispr Enzymes And Systems
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Targeting Disorders And Diseases Using Viral Components
  • Exosome Isolation
  • Methods Of Producing And Using Exersomes And Bioengineered Exersomes
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Hbv And Viral Diseases And Disorders
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Targeting Disorders And Diseases Using Particle Delivery Components
  • Two-Tailed Self-Delivering Sirna
  • Novel Crispr Enzymes And Systems
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Hbv And Viral Diseases And Disorders
  • Exosomal Loading Using Hydrophobically Modified Oligonucleotides
  • Compositions And Methods Of Use Of Crispr-Cas Systems In Nucleotide Repeat Disorders
  • Crispr Enzyme Mutations Reducing Off-Target Effects
  • Delivery, Engineering And Optimization Of Tandem Guide Systems, Methods And Compositions For Sequence Manipulation
  • Type Vi Crispr Orthologs And Systems
  • Compositions And Methods Of Use Of Crispr-Cas Systems In Nucleotide Repeat Disorders
  • Protected Guide Rnas (Pgrnas)
  • Novel Crispr Enzymes And Systems
  • 3-D Collagen Scaffold-Generated Exosomes And Uses Thereof
  • Engineering And Optimization Of Improved Systems, Methods And Enzyme Compositions For Sequence Manipulation
  • Compositions And Methods Of Use Of Crispr-Cas Systems In Nucleotide Repeat Disorders
  • Exosomal Loading Using Hydrophobically Modified Oligonucleotides
  • Combination For Treating An Inflammatory Disorder
  • Novel Cas13b Orthologues Crispr Enzymes And Systems
  • Novel Type Vi Crispr Orthologs And Systems
  • Modulation Of Novel Immune Checkpoint Targets
  • Platforms, Compositions, And Methods For Therapeutic Delivery
  • Compositions And Methods For Evaluating And Modulating Immune Responses
  • Crispr-Associated Transposase Systems And Methods Of Use Thereof
  • Crispr Having Or Associated With Destabilization Domains
  • Exosomal Loading Using Hydrophobically Modified Oligonucleotides
  • Novel Crispr Enzymes And Systems
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2012.131

    DOI

    http://dx.doi.org/10.1038/nprot.2012.131

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1017288792

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23154783


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brain", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Exosomes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Silencing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Small Interfering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden", 
              "id": "http://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK", 
                "Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "El-Andaloussi", 
            "givenName": "Samir", 
            "id": "sg:person.07535165677.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07535165677.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.4991.5", 
              "name": [
                "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Yi", 
            "id": "sg:person.0772225071.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772225071.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.4991.5", 
              "name": [
                "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lakhal-Littleton", 
            "givenName": "Samira", 
            "id": "sg:person.0577307112.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577307112.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.4991.5", 
              "name": [
                "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Jinghuan", 
            "id": "sg:person.0611570632.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611570632.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Engineering Laboratory, Proteos, Agency for Science Technology and Research, Singapore, Singapore", 
              "id": "http://www.grid.ac/institutes/grid.185448.4", 
              "name": [
                "Molecular Engineering Laboratory, Proteos, Agency for Science Technology and Research, Singapore, Singapore"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Seow", 
            "givenName": "Yiqi", 
            "id": "sg:person.01055675676.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055675676.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.8348.7", 
              "name": [
                "Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gardiner", 
            "givenName": "Chris", 
            "id": "sg:person.01241645654.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241645654.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University Department of Clinical Neurosciences, Institute of Neurology, University College London, London, UK", 
              "id": "http://www.grid.ac/institutes/grid.83440.3b", 
              "name": [
                "University Department of Clinical Neurosciences, Institute of Neurology, University College London, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Alvarez-Erviti", 
            "givenName": "Lydia", 
            "id": "sg:person.01021245506.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021245506.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.8348.7", 
              "name": [
                "Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sargent", 
            "givenName": "Ian L", 
            "id": "sg:person.01016306474.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016306474.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.4991.5", 
              "name": [
                "Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wood", 
            "givenName": "Matthew J A", 
            "id": "sg:person.014256626047.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014256626047.55"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt.1807", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036478917", 
              "https://doi.org/10.1038/nbt.1807"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms1285", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010814909", 
              "https://doi.org/10.1038/ncomms1285"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00262-005-0075-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030793102", 
              "https://doi.org/10.1007/s00262-005-0075-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.leu.2404132", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046811448", 
              "https://doi.org/10.1038/sj.leu.2404132"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1929", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001261659", 
              "https://doi.org/10.1038/ncb1929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrd3459", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019933275", 
              "https://doi.org/10.1038/nrd3459"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1596", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001718862", 
              "https://doi.org/10.1038/ncb1596"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2012-11-15", 
        "datePublishedReg": "2012-11-15", 
        "description": "The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused with a peptide ligand. Next, we explain how to purify and characterize exosomes from transfected cell supernatant. Next, we detail crucial steps for loading siRNA into exosomes. Finally, we outline how to use exosomes to efficiently deliver siRNA in vitro and in vivo in mouse brain. Examples of anticipated results in which exosome-mediated siRNA delivery is evaluated by functional assays and imaging are also provided. The entire protocol takes \u223c3 weeks.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nprot.2012.131", 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2766146", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5148980", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5146732", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2756612", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3636620", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1037502", 
            "issn": [
              "1754-2189", 
              "1750-2799"
            ], 
            "name": "Nature Protocols", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "12", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "keywords": [
          "delivery of siRNA", 
          "small interfering RNAs", 
          "tissue-specific delivery", 
          "different biological barriers", 
          "siRNA delivery", 
          "biological barriers", 
          "drug delivery", 
          "gene silencing", 
          "interfering RNAs", 
          "expression vector", 
          "exosomal proteins", 
          "cultured cells", 
          "functional assays", 
          "exosomes", 
          "new avenues", 
          "drug discovery", 
          "delivery", 
          "peptide ligands", 
          "entire protocol", 
          "mouse brain", 
          "promising tool", 
          "protocol", 
          "vitro", 
          "vivo", 
          "cell supernatants", 
          "therapeutic potential", 
          "silencing", 
          "crucial step", 
          "RNA", 
          "protein", 
          "transfection", 
          "cells", 
          "assays", 
          "discovery", 
          "vector", 
          "avenues", 
          "ligands", 
          "supernatant", 
          "potential", 
          "generation", 
          "imaging", 
          "brain", 
          "step", 
          "use", 
          "tool", 
          "barriers", 
          "results", 
          "example", 
          "weeks"
        ], 
        "name": "Exosome-mediated delivery of siRNA in vitro and in vivo", 
        "pagination": "2112-2126", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1017288792"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nprot.2012.131"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "23154783"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nprot.2012.131", 
          "https://app.dimensions.ai/details/publication/pub.1017288792"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:30", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_571.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nprot.2012.131"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2012.131'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2012.131'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2012.131'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2012.131'


     

    This table displays all metadata directly associated to this object as RDF triples.

    253 TRIPLES      21 PREDICATES      90 URIs      75 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nprot.2012.131 schema:about N07f2a1d63dae44d5b8c7b8ba3858cb89
    2 N13f0a0ce20fe443e8c8145cb604dea93
    3 N4cf90467ce354a18b21ce42389e190c4
    4 N4f5459061c3f4dfe9bc96b641c50d463
    5 N9a285b5b5af44e7ea8a44091aaac0ec7
    6 Na82020333f964beebb66be626cbb39b6
    7 Nd1f226d49bed4cb5843074dfd11f544d
    8 Ne14efb49e9604f57a1c2fc0c74d14992
    9 Nee9e2d8c6958478e8da9fcaafcec5ef5
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author N0acd13a3550642159d610dd5aaebf099
    13 schema:citation sg:pub.10.1007/s00262-005-0075-9
    14 sg:pub.10.1038/nbt.1807
    15 sg:pub.10.1038/ncb1596
    16 sg:pub.10.1038/ncb1929
    17 sg:pub.10.1038/ncomms1285
    18 sg:pub.10.1038/nrd3459
    19 sg:pub.10.1038/sj.leu.2404132
    20 schema:datePublished 2012-11-15
    21 schema:datePublishedReg 2012-11-15
    22 schema:description The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused with a peptide ligand. Next, we explain how to purify and characterize exosomes from transfected cell supernatant. Next, we detail crucial steps for loading siRNA into exosomes. Finally, we outline how to use exosomes to efficiently deliver siRNA in vitro and in vivo in mouse brain. Examples of anticipated results in which exosome-mediated siRNA delivery is evaluated by functional assays and imaging are also provided. The entire protocol takes ∼3 weeks.
    23 schema:genre article
    24 schema:isAccessibleForFree false
    25 schema:isPartOf Nb3d5e48bdb6e46329616a15c14ab01f8
    26 Ne9f8bcfb4ded4aecbfc8bf94132efe91
    27 sg:journal.1037502
    28 schema:keywords RNA
    29 assays
    30 avenues
    31 barriers
    32 biological barriers
    33 brain
    34 cell supernatants
    35 cells
    36 crucial step
    37 cultured cells
    38 delivery
    39 delivery of siRNA
    40 different biological barriers
    41 discovery
    42 drug delivery
    43 drug discovery
    44 entire protocol
    45 example
    46 exosomal proteins
    47 exosomes
    48 expression vector
    49 functional assays
    50 gene silencing
    51 generation
    52 imaging
    53 interfering RNAs
    54 ligands
    55 mouse brain
    56 new avenues
    57 peptide ligands
    58 potential
    59 promising tool
    60 protein
    61 protocol
    62 results
    63 siRNA delivery
    64 silencing
    65 small interfering RNAs
    66 step
    67 supernatant
    68 therapeutic potential
    69 tissue-specific delivery
    70 tool
    71 transfection
    72 use
    73 vector
    74 vitro
    75 vivo
    76 weeks
    77 schema:name Exosome-mediated delivery of siRNA in vitro and in vivo
    78 schema:pagination 2112-2126
    79 schema:productId N7ab4d6c6ec864645baceb0319cc57b90
    80 N7f7ac488cc0549f6b1ffa55b5358cd06
    81 Ndaaf6cad637b48738b17f2dafd7f7031
    82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017288792
    83 https://doi.org/10.1038/nprot.2012.131
    84 schema:sdDatePublished 2022-12-01T06:30
    85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    86 schema:sdPublisher N5c98377fcbc14a279db4530d3cfbbae3
    87 schema:url https://doi.org/10.1038/nprot.2012.131
    88 sgo:license sg:explorer/license/
    89 sgo:sdDataset articles
    90 rdf:type schema:ScholarlyArticle
    91 N07f2a1d63dae44d5b8c7b8ba3858cb89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Genetic Vectors
    93 rdf:type schema:DefinedTerm
    94 N0acd13a3550642159d610dd5aaebf099 rdf:first sg:person.07535165677.56
    95 rdf:rest N6b7fccb40ad34126808075874982a758
    96 N1212206b300043608aa3bce36659cfb8 rdf:first sg:person.014256626047.55
    97 rdf:rest rdf:nil
    98 N13f0a0ce20fe443e8c8145cb604dea93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Brain
    100 rdf:type schema:DefinedTerm
    101 N4cf90467ce354a18b21ce42389e190c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name RNA, Small Interfering
    103 rdf:type schema:DefinedTerm
    104 N4f5459061c3f4dfe9bc96b641c50d463 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Exosomes
    106 rdf:type schema:DefinedTerm
    107 N5c98377fcbc14a279db4530d3cfbbae3 schema:name Springer Nature - SN SciGraph project
    108 rdf:type schema:Organization
    109 N68b3bf745f2b4f85aca8d315e95188ff rdf:first sg:person.01016306474.64
    110 rdf:rest N1212206b300043608aa3bce36659cfb8
    111 N6b7fccb40ad34126808075874982a758 rdf:first sg:person.0772225071.64
    112 rdf:rest Naf5b907c2fb245be96d660725da69bfe
    113 N7ab4d6c6ec864645baceb0319cc57b90 schema:name pubmed_id
    114 schema:value 23154783
    115 rdf:type schema:PropertyValue
    116 N7f7ac488cc0549f6b1ffa55b5358cd06 schema:name doi
    117 schema:value 10.1038/nprot.2012.131
    118 rdf:type schema:PropertyValue
    119 N9a285b5b5af44e7ea8a44091aaac0ec7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Animals
    121 rdf:type schema:DefinedTerm
    122 N9e5f76813a814008bfdf46153469fba1 rdf:first sg:person.01055675676.02
    123 rdf:rest Ndd83b77bef5a40f9adb726e179b38e98
    124 Na71b1436a3b048f9b84def4754344be7 rdf:first sg:person.0611570632.25
    125 rdf:rest N9e5f76813a814008bfdf46153469fba1
    126 Na82020333f964beebb66be626cbb39b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Transfection
    128 rdf:type schema:DefinedTerm
    129 Naf5b907c2fb245be96d660725da69bfe rdf:first sg:person.0577307112.01
    130 rdf:rest Na71b1436a3b048f9b84def4754344be7
    131 Nb3d5e48bdb6e46329616a15c14ab01f8 schema:volumeNumber 7
    132 rdf:type schema:PublicationVolume
    133 Nd1f226d49bed4cb5843074dfd11f544d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Gene Silencing
    135 rdf:type schema:DefinedTerm
    136 Nd7de9ba098314e7cb848cfc94c19957b rdf:first sg:person.01021245506.19
    137 rdf:rest N68b3bf745f2b4f85aca8d315e95188ff
    138 Ndaaf6cad637b48738b17f2dafd7f7031 schema:name dimensions_id
    139 schema:value pub.1017288792
    140 rdf:type schema:PropertyValue
    141 Ndd83b77bef5a40f9adb726e179b38e98 rdf:first sg:person.01241645654.42
    142 rdf:rest Nd7de9ba098314e7cb848cfc94c19957b
    143 Ne14efb49e9604f57a1c2fc0c74d14992 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Mice
    145 rdf:type schema:DefinedTerm
    146 Ne9f8bcfb4ded4aecbfc8bf94132efe91 schema:issueNumber 12
    147 rdf:type schema:PublicationIssue
    148 Nee9e2d8c6958478e8da9fcaafcec5ef5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Gene Transfer Techniques
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Biological Sciences
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Biochemistry and Cell Biology
    156 rdf:type schema:DefinedTerm
    157 sg:grant.2756612 http://pending.schema.org/fundedItem sg:pub.10.1038/nprot.2012.131
    158 rdf:type schema:MonetaryGrant
    159 sg:grant.2766146 http://pending.schema.org/fundedItem sg:pub.10.1038/nprot.2012.131
    160 rdf:type schema:MonetaryGrant
    161 sg:grant.3636620 http://pending.schema.org/fundedItem sg:pub.10.1038/nprot.2012.131
    162 rdf:type schema:MonetaryGrant
    163 sg:grant.5146732 http://pending.schema.org/fundedItem sg:pub.10.1038/nprot.2012.131
    164 rdf:type schema:MonetaryGrant
    165 sg:grant.5148980 http://pending.schema.org/fundedItem sg:pub.10.1038/nprot.2012.131
    166 rdf:type schema:MonetaryGrant
    167 sg:journal.1037502 schema:issn 1750-2799
    168 1754-2189
    169 schema:name Nature Protocols
    170 schema:publisher Springer Nature
    171 rdf:type schema:Periodical
    172 sg:person.01016306474.64 schema:affiliation grid-institutes:grid.8348.7
    173 schema:familyName Sargent
    174 schema:givenName Ian L
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016306474.64
    176 rdf:type schema:Person
    177 sg:person.01021245506.19 schema:affiliation grid-institutes:grid.83440.3b
    178 schema:familyName Alvarez-Erviti
    179 schema:givenName Lydia
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021245506.19
    181 rdf:type schema:Person
    182 sg:person.01055675676.02 schema:affiliation grid-institutes:grid.185448.4
    183 schema:familyName Seow
    184 schema:givenName Yiqi
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055675676.02
    186 rdf:type schema:Person
    187 sg:person.01241645654.42 schema:affiliation grid-institutes:grid.8348.7
    188 schema:familyName Gardiner
    189 schema:givenName Chris
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241645654.42
    191 rdf:type schema:Person
    192 sg:person.014256626047.55 schema:affiliation grid-institutes:grid.4991.5
    193 schema:familyName Wood
    194 schema:givenName Matthew J A
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014256626047.55
    196 rdf:type schema:Person
    197 sg:person.0577307112.01 schema:affiliation grid-institutes:grid.4991.5
    198 schema:familyName Lakhal-Littleton
    199 schema:givenName Samira
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577307112.01
    201 rdf:type schema:Person
    202 sg:person.0611570632.25 schema:affiliation grid-institutes:grid.4991.5
    203 schema:familyName Li
    204 schema:givenName Jinghuan
    205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611570632.25
    206 rdf:type schema:Person
    207 sg:person.07535165677.56 schema:affiliation grid-institutes:grid.4714.6
    208 schema:familyName El-Andaloussi
    209 schema:givenName Samir
    210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07535165677.56
    211 rdf:type schema:Person
    212 sg:person.0772225071.64 schema:affiliation grid-institutes:grid.4991.5
    213 schema:familyName Lee
    214 schema:givenName Yi
    215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772225071.64
    216 rdf:type schema:Person
    217 sg:pub.10.1007/s00262-005-0075-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030793102
    218 https://doi.org/10.1007/s00262-005-0075-9
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/nbt.1807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036478917
    221 https://doi.org/10.1038/nbt.1807
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/ncb1596 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001718862
    224 https://doi.org/10.1038/ncb1596
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1038/ncb1929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001261659
    227 https://doi.org/10.1038/ncb1929
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/ncomms1285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010814909
    230 https://doi.org/10.1038/ncomms1285
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1038/nrd3459 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019933275
    233 https://doi.org/10.1038/nrd3459
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/sj.leu.2404132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046811448
    236 https://doi.org/10.1038/sj.leu.2404132
    237 rdf:type schema:CreativeWork
    238 grid-institutes:grid.185448.4 schema:alternateName Molecular Engineering Laboratory, Proteos, Agency for Science Technology and Research, Singapore, Singapore
    239 schema:name Molecular Engineering Laboratory, Proteos, Agency for Science Technology and Research, Singapore, Singapore
    240 rdf:type schema:Organization
    241 grid-institutes:grid.4714.6 schema:alternateName Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden
    242 schema:name Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden
    243 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    244 rdf:type schema:Organization
    245 grid-institutes:grid.4991.5 schema:alternateName Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    246 schema:name Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    247 rdf:type schema:Organization
    248 grid-institutes:grid.83440.3b schema:alternateName University Department of Clinical Neurosciences, Institute of Neurology, University College London, London, UK
    249 schema:name University Department of Clinical Neurosciences, Institute of Neurology, University College London, London, UK
    250 rdf:type schema:Organization
    251 grid-institutes:grid.8348.7 schema:alternateName Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK
    252 schema:name Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK
    253 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...