Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-07

AUTHORS

Warwick B Dunn, David Broadhurst, Paul Begley, Eva Zelena, Sue Francis-McIntyre, Nadine Anderson, Marie Brown, Joshau D Knowles, Antony Halsall, John N Haselden, Andrew W Nicholls, Ian D Wilson, Douglas B Kell, Royston Goodacre, The Human Serum Metabolome Consortium

ABSTRACT

Metabolism has an essential role in biological systems. Identification and quantitation of the compounds in the metabolome is defined as metabolic profiling, and it is applied to define metabolic changes related to genetic differences, environmental influences and disease or drug perturbations. Chromatography-mass spectrometry (MS) platforms are frequently used to provide the sensitive and reproducible detection of hundreds to thousands of metabolites in a single biofluid or tissue sample. Here we describe the experimental workflow for long-term and large-scale metabolomic studies involving thousands of human samples with data acquired for multiple analytical batches over many months and years. Protocols for serum- and plasma-based metabolic profiling applying gas chromatography-MS (GC-MS) and ultraperformance liquid chromatography-MS (UPLC-MS) are described. These include biofluid collection, sample preparation, data acquisition, data pre-processing and quality assurance. Methods for quality control-based robust LOESS signal correction to provide signal correction and integration of data from multiple analytical batches are also described. More... »

PAGES

1060

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2011.335

    DOI

    http://dx.doi.org/10.1038/nprot.2011.335

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015396743

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21720319


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