Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-08-12

AUTHORS

Mara E Pitulescu, Inga Schmidt, Rui Benedito, Ralf H Adams

ABSTRACT

The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The characterization of physiological and pathological angiogenic processes in mice has been greatly facilitated by inducible and cell type–specific loss-of-function and gain-of-function genetics. In this paper, we provide a detailed protocol for tamoxifen-inducible gene deletion in neonatal mice, as well as for retina dissection, whole-mount immunostaining and the quantitation of EC sprouting and proliferation. These methods have been optimized by our laboratory and yield reliable results. The entire protocol takes ~10 d to complete. More... »

PAGES

1518-1534

References to SciGraph publications

  • 1988-03. Lectin histochemistry of gangliosidosis in ACTA NEUROPATHOLOGICA
  • 2005-12-14. Retinal angiogenesis in development and disease in NATURE
  • 2009-10-08. Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis in NATURE PROTOCOLS
  • 2004. High-Resolution in Situ Confocal Analysis of Endothelial Cells in METHODS IN ENDOTHELIAL CELL BIOLOGY
  • 2010-05-05. Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis in NATURE
  • 1990-02. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three-dimensional cultures of rat aorta: A comparative study of angiogenesis in Matrigel, collagen, fibrin, and plasma clot in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 2003-12-14. Hippocampal plasticity requires postsynaptic ephrinBs in NATURE NEUROSCIENCE
  • 2009-12-03. Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices in NATURE PROTOCOLS
  • 1980-06. Technical report—a new chamber technique for microvascular studies in unanesthetized hamsters in RESEARCH IN EXPERIMENTAL MEDICINE
  • 2009-07-30. Spheroid-based human endothelial cell microvessel formation in vivo in NATURE PROTOCOLS
  • 2010-05-27. Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis in NATURE
  • 1987-04. Griffonia simplicifolia lectins bind specifically to endothelial cells and some epithelial cells in mouse tissues in JOURNAL OF MOLECULAR HISTOLOGY
  • 2001-03-27. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus in BMC DEVELOPMENTAL BIOLOGY
  • 2009-02-12. Molecular differentiation and specialization of vascular beds in ANGIOGENESIS
  • 1980-12. Adult tissues contain chemo-attractants for vascular endothelial cells in NATURE
  • 2008-04-06. Spheroid-based engineering of a human vasculature in mice in NATURE METHODS
  • 2007-06. Molecular regulation of angiogenesis and lymphangiogenesis in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2010.113

    DOI

    http://dx.doi.org/10.1038/nprot.2010.113

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1016756802

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20725067


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Proliferation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dissection", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endothelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Deletion", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Targeting", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunohistochemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Inbred C57BL", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Transgenic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neovascularization, Physiologic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Lectins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Retina", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Retinal Vessels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tamoxifen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "beta-Galactosidase", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany", 
              "id": "http://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pitulescu", 
            "givenName": "Mara E", 
            "id": "sg:person.01146442273.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146442273.21"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany", 
              "id": "http://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schmidt", 
            "givenName": "Inga", 
            "id": "sg:person.01247154164.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247154164.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany", 
              "id": "http://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Benedito", 
            "givenName": "Rui", 
            "id": "sg:person.01077553426.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077553426.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany", 
              "id": "http://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Adams", 
            "givenName": "Ralf H", 
            "id": "sg:person.0702247561.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702247561.03"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature08995", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041374258", 
              "https://doi.org/10.1038/nature08995"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2009.96", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029339218", 
              "https://doi.org/10.1038/nprot.2009.96"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-18725-4_28", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011836771", 
              "https://doi.org/10.1007/978-3-642-18725-4_28"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00688094", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019131163", 
              "https://doi.org/10.1007/bf00688094"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2009.187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048155602", 
              "https://doi.org/10.1038/nprot.2009.187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1164", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051258574", 
              "https://doi.org/10.1038/nn1164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01680633", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003861450", 
              "https://doi.org/10.1007/bf01680633"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04482", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010896038", 
              "https://doi.org/10.1038/nature04482"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/288483a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010582862", 
              "https://doi.org/10.1038/288483a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm2183", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049496060", 
              "https://doi.org/10.1038/nrm2183"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01851841", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003146441", 
              "https://doi.org/10.1007/bf01851841"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016200527", 
              "https://doi.org/10.1038/nmeth.1198"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02624102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013459818", 
              "https://doi.org/10.1007/bf02624102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10456-009-9132-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049599620", 
              "https://doi.org/10.1007/s10456-009-9132-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2009.221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034681287", 
              "https://doi.org/10.1038/nprot.2009.221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-213x-1-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042905963", 
              "https://doi.org/10.1186/1471-213x-1-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028883419", 
              "https://doi.org/10.1038/nature09002"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-08-12", 
        "datePublishedReg": "2010-08-12", 
        "description": "The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The characterization of physiological and pathological angiogenic processes in mice has been greatly facilitated by inducible and cell type\u2013specific loss-of-function and gain-of-function genetics. In this paper, we provide a detailed protocol for tamoxifen-inducible gene deletion in neonatal mice, as well as for retina dissection, whole-mount immunostaining and the quantitation of EC sprouting and proliferation. These methods have been optimized by our laboratory and yield reliable results. The entire protocol takes ~10 d to complete.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nprot.2010.113", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1037502", 
            "issn": [
              "1754-2189", 
              "1750-2799"
            ], 
            "name": "Nature Protocols", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "9", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "5"
          }
        ], 
        "keywords": [
          "angiogenic blood vessel growth", 
          "powerful experimental system", 
          "perivascular cell recruitment", 
          "cell type-specific loss", 
          "pathological angiogenic processes", 
          "whole-mount immunostaining", 
          "function genetics", 
          "inducible genes", 
          "postnatal organism", 
          "detailed protocol", 
          "blood vessel growth", 
          "endothelial cell proliferation", 
          "gene deletion", 
          "cell proliferation", 
          "EC sprouting", 
          "three-dimensional architecture", 
          "vessel growth", 
          "angiogenic process", 
          "neonatal vasculature", 
          "vessel remodeling", 
          "proliferation", 
          "cell recruitment", 
          "retinal angiogenesis", 
          "experimental system", 
          "genes", 
          "vessel network", 
          "sprouting", 
          "genetics", 
          "entire protocol", 
          "organisms", 
          "deletion", 
          "mice", 
          "maturation", 
          "recruitment", 
          "remodeling", 
          "angiogenesis", 
          "neonatal mice", 
          "growth", 
          "characterization", 
          "high resolution", 
          "function", 
          "analysis", 
          "process", 
          "immunostaining", 
          "vasculature", 
          "loss", 
          "retina", 
          "quantitation", 
          "protocol", 
          "laboratory", 
          "dissection", 
          "architecture", 
          "resolution", 
          "results", 
          "network", 
          "system", 
          "gain", 
          "reliable results", 
          "method", 
          "paper"
        ], 
        "name": "Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice", 
        "pagination": "1518-1534", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1016756802"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nprot.2010.113"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "20725067"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nprot.2010.113", 
          "https://app.dimensions.ai/details/publication/pub.1016756802"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:28", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_499.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nprot.2010.113"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2010.113'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2010.113'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2010.113'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2010.113'


     

    This table displays all metadata directly associated to this object as RDF triples.

    278 TRIPLES      21 PREDICATES      119 URIs      94 LITERALS      24 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nprot.2010.113 schema:about N0165c28348fa4dbe904e36da748dbc29
    2 N117fd1ce8c504cfda475fda3c89de44f
    3 N18e08a23bef84b22aac74293911b3162
    4 N20c5244423b74a00ae9abc2ef3d1d75e
    5 N3b454fc1f64e4458baca825665873700
    6 N4696612ea1f345d59ee48782383af3f7
    7 N6f48bed51bd04cab95b37bd1641c5132
    8 N73f583ccae2b4a6094ef909a073ad110
    9 N92568c60115249a49165a796915c65df
    10 N9896fbfd655d402a8da58f7e0052c098
    11 N9b4986efb11f47b887cf619207e625e8
    12 N9c94ecf635af4577bcbf7b7ad527fd50
    13 Nb098e7d28ab84133acec4298708c5656
    14 Ncad4152dd24c403c80565a325e85a5fd
    15 Ndb07c00800ef428e9b8d6f7cff3cf83f
    16 Ndd1b097c56574b478d5f9f2a64a5fb36
    17 Ne9aa627ffada401dbb00f8a190f6b5e1
    18 anzsrc-for:06
    19 anzsrc-for:0604
    20 schema:author N9740953185a040cbb9ac990df4f59063
    21 schema:citation sg:pub.10.1007/978-3-642-18725-4_28
    22 sg:pub.10.1007/bf00688094
    23 sg:pub.10.1007/bf01680633
    24 sg:pub.10.1007/bf01851841
    25 sg:pub.10.1007/bf02624102
    26 sg:pub.10.1007/s10456-009-9132-x
    27 sg:pub.10.1038/288483a0
    28 sg:pub.10.1038/nature04482
    29 sg:pub.10.1038/nature08995
    30 sg:pub.10.1038/nature09002
    31 sg:pub.10.1038/nmeth.1198
    32 sg:pub.10.1038/nn1164
    33 sg:pub.10.1038/nprot.2009.187
    34 sg:pub.10.1038/nprot.2009.221
    35 sg:pub.10.1038/nprot.2009.96
    36 sg:pub.10.1038/nrm2183
    37 sg:pub.10.1186/1471-213x-1-4
    38 schema:datePublished 2010-08-12
    39 schema:datePublishedReg 2010-08-12
    40 schema:description The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The characterization of physiological and pathological angiogenic processes in mice has been greatly facilitated by inducible and cell type–specific loss-of-function and gain-of-function genetics. In this paper, we provide a detailed protocol for tamoxifen-inducible gene deletion in neonatal mice, as well as for retina dissection, whole-mount immunostaining and the quantitation of EC sprouting and proliferation. These methods have been optimized by our laboratory and yield reliable results. The entire protocol takes ~10 d to complete.
    41 schema:genre article
    42 schema:isAccessibleForFree false
    43 schema:isPartOf N388e61cff3a4467c8134d980e8dd42d4
    44 Ndeaa2e06662d445eb692b0cf733389b4
    45 sg:journal.1037502
    46 schema:keywords EC sprouting
    47 analysis
    48 angiogenesis
    49 angiogenic blood vessel growth
    50 angiogenic process
    51 architecture
    52 blood vessel growth
    53 cell proliferation
    54 cell recruitment
    55 cell type-specific loss
    56 characterization
    57 deletion
    58 detailed protocol
    59 dissection
    60 endothelial cell proliferation
    61 entire protocol
    62 experimental system
    63 function
    64 function genetics
    65 gain
    66 gene deletion
    67 genes
    68 genetics
    69 growth
    70 high resolution
    71 immunostaining
    72 inducible genes
    73 laboratory
    74 loss
    75 maturation
    76 method
    77 mice
    78 neonatal mice
    79 neonatal vasculature
    80 network
    81 organisms
    82 paper
    83 pathological angiogenic processes
    84 perivascular cell recruitment
    85 postnatal organism
    86 powerful experimental system
    87 process
    88 proliferation
    89 protocol
    90 quantitation
    91 recruitment
    92 reliable results
    93 remodeling
    94 resolution
    95 results
    96 retina
    97 retinal angiogenesis
    98 sprouting
    99 system
    100 three-dimensional architecture
    101 vasculature
    102 vessel growth
    103 vessel network
    104 vessel remodeling
    105 whole-mount immunostaining
    106 schema:name Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice
    107 schema:pagination 1518-1534
    108 schema:productId N2f559152c9fd439fb151421385d615c1
    109 N38e66ec9510f47a0823fc7fe328eec89
    110 N98d5a45098b947fdacd33690384754f7
    111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016756802
    112 https://doi.org/10.1038/nprot.2010.113
    113 schema:sdDatePublished 2022-12-01T06:28
    114 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    115 schema:sdPublisher N2a0a40c2624347f9acd5769bd1a61c75
    116 schema:url https://doi.org/10.1038/nprot.2010.113
    117 sgo:license sg:explorer/license/
    118 sgo:sdDataset articles
    119 rdf:type schema:ScholarlyArticle
    120 N0165c28348fa4dbe904e36da748dbc29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Retinal Vessels
    122 rdf:type schema:DefinedTerm
    123 N117fd1ce8c504cfda475fda3c89de44f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Retina
    125 rdf:type schema:DefinedTerm
    126 N18e08a23bef84b22aac74293911b3162 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Gene Deletion
    128 rdf:type schema:DefinedTerm
    129 N1978822a7bef4986ac823d4a57150629 rdf:first sg:person.01247154164.00
    130 rdf:rest N523f09ecff734c7cbc86db098d21ac0a
    131 N20c5244423b74a00ae9abc2ef3d1d75e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Plant Lectins
    133 rdf:type schema:DefinedTerm
    134 N2a0a40c2624347f9acd5769bd1a61c75 schema:name Springer Nature - SN SciGraph project
    135 rdf:type schema:Organization
    136 N2f559152c9fd439fb151421385d615c1 schema:name doi
    137 schema:value 10.1038/nprot.2010.113
    138 rdf:type schema:PropertyValue
    139 N388e61cff3a4467c8134d980e8dd42d4 schema:volumeNumber 5
    140 rdf:type schema:PublicationVolume
    141 N38e66ec9510f47a0823fc7fe328eec89 schema:name dimensions_id
    142 schema:value pub.1016756802
    143 rdf:type schema:PropertyValue
    144 N3b454fc1f64e4458baca825665873700 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Mice, Inbred C57BL
    146 rdf:type schema:DefinedTerm
    147 N4696612ea1f345d59ee48782383af3f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Mice
    149 rdf:type schema:DefinedTerm
    150 N523f09ecff734c7cbc86db098d21ac0a rdf:first sg:person.01077553426.30
    151 rdf:rest Nfc6e45f47a874228b73da88232a6cb83
    152 N6f48bed51bd04cab95b37bd1641c5132 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Endothelial Cells
    154 rdf:type schema:DefinedTerm
    155 N73f583ccae2b4a6094ef909a073ad110 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Neovascularization, Physiologic
    157 rdf:type schema:DefinedTerm
    158 N92568c60115249a49165a796915c65df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Mice, Transgenic
    160 rdf:type schema:DefinedTerm
    161 N9740953185a040cbb9ac990df4f59063 rdf:first sg:person.01146442273.21
    162 rdf:rest N1978822a7bef4986ac823d4a57150629
    163 N9896fbfd655d402a8da58f7e0052c098 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Microscopy, Fluorescence
    165 rdf:type schema:DefinedTerm
    166 N98d5a45098b947fdacd33690384754f7 schema:name pubmed_id
    167 schema:value 20725067
    168 rdf:type schema:PropertyValue
    169 N9b4986efb11f47b887cf619207e625e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name beta-Galactosidase
    171 rdf:type schema:DefinedTerm
    172 N9c94ecf635af4577bcbf7b7ad527fd50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    173 schema:name Immunohistochemistry
    174 rdf:type schema:DefinedTerm
    175 Nb098e7d28ab84133acec4298708c5656 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name Cell Proliferation
    177 rdf:type schema:DefinedTerm
    178 Ncad4152dd24c403c80565a325e85a5fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Gene Targeting
    180 rdf:type schema:DefinedTerm
    181 Ndb07c00800ef428e9b8d6f7cff3cf83f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Tamoxifen
    183 rdf:type schema:DefinedTerm
    184 Ndd1b097c56574b478d5f9f2a64a5fb36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Animals
    186 rdf:type schema:DefinedTerm
    187 Ndeaa2e06662d445eb692b0cf733389b4 schema:issueNumber 9
    188 rdf:type schema:PublicationIssue
    189 Ne9aa627ffada401dbb00f8a190f6b5e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Dissection
    191 rdf:type schema:DefinedTerm
    192 Nfc6e45f47a874228b73da88232a6cb83 rdf:first sg:person.0702247561.03
    193 rdf:rest rdf:nil
    194 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    195 schema:name Biological Sciences
    196 rdf:type schema:DefinedTerm
    197 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    198 schema:name Genetics
    199 rdf:type schema:DefinedTerm
    200 sg:journal.1037502 schema:issn 1750-2799
    201 1754-2189
    202 schema:name Nature Protocols
    203 schema:publisher Springer Nature
    204 rdf:type schema:Periodical
    205 sg:person.01077553426.30 schema:affiliation grid-institutes:grid.5949.1
    206 schema:familyName Benedito
    207 schema:givenName Rui
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077553426.30
    209 rdf:type schema:Person
    210 sg:person.01146442273.21 schema:affiliation grid-institutes:grid.5949.1
    211 schema:familyName Pitulescu
    212 schema:givenName Mara E
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146442273.21
    214 rdf:type schema:Person
    215 sg:person.01247154164.00 schema:affiliation grid-institutes:grid.5949.1
    216 schema:familyName Schmidt
    217 schema:givenName Inga
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247154164.00
    219 rdf:type schema:Person
    220 sg:person.0702247561.03 schema:affiliation grid-institutes:grid.5949.1
    221 schema:familyName Adams
    222 schema:givenName Ralf H
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702247561.03
    224 rdf:type schema:Person
    225 sg:pub.10.1007/978-3-642-18725-4_28 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011836771
    226 https://doi.org/10.1007/978-3-642-18725-4_28
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1007/bf00688094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019131163
    229 https://doi.org/10.1007/bf00688094
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1007/bf01680633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003861450
    232 https://doi.org/10.1007/bf01680633
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1007/bf01851841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003146441
    235 https://doi.org/10.1007/bf01851841
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1007/bf02624102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013459818
    238 https://doi.org/10.1007/bf02624102
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1007/s10456-009-9132-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049599620
    241 https://doi.org/10.1007/s10456-009-9132-x
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/288483a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010582862
    244 https://doi.org/10.1038/288483a0
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/nature04482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010896038
    247 https://doi.org/10.1038/nature04482
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/nature08995 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041374258
    250 https://doi.org/10.1038/nature08995
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nature09002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028883419
    253 https://doi.org/10.1038/nature09002
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nmeth.1198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016200527
    256 https://doi.org/10.1038/nmeth.1198
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/nn1164 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051258574
    259 https://doi.org/10.1038/nn1164
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nprot.2009.187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048155602
    262 https://doi.org/10.1038/nprot.2009.187
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nprot.2009.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034681287
    265 https://doi.org/10.1038/nprot.2009.221
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nprot.2009.96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029339218
    268 https://doi.org/10.1038/nprot.2009.96
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/nrm2183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049496060
    271 https://doi.org/10.1038/nrm2183
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1186/1471-213x-1-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042905963
    274 https://doi.org/10.1186/1471-213x-1-4
    275 rdf:type schema:CreativeWork
    276 grid-institutes:grid.5949.1 schema:alternateName Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of Münster, Münster, Germany
    277 schema:name Department of Tissue Morphogenesis, Faculty of Medicine, Max Planck Institute for Molecular Biomedicine, University of Münster, Münster, Germany
    278 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...