Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-08-12

AUTHORS

Mara E Pitulescu, Inga Schmidt, Rui Benedito, Ralf H Adams

ABSTRACT

The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The characterization of physiological and pathological angiogenic processes in mice has been greatly facilitated by inducible and cell type–specific loss-of-function and gain-of-function genetics. In this paper, we provide a detailed protocol for tamoxifen-inducible gene deletion in neonatal mice, as well as for retina dissection, whole-mount immunostaining and the quantitation of EC sprouting and proliferation. These methods have been optimized by our laboratory and yield reliable results. The entire protocol takes ~10 d to complete. More... »

PAGES

1518-1534

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2010.113

    DOI

    http://dx.doi.org/10.1038/nprot.2010.113

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1016756802

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20725067


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