A protocol for efficiently retrieving and characterizing flanking sequence tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-04-09

AUTHORS

Vera Thole, Sílvia C Alves, Barbara Worland, Michael W Bevan, Philippe Vain

ABSTRACT

Brachypodium distachyon is emerging as a new model system for bridging research into temperate cereal crops, such as wheat and barley, and for promoting research in novel biomass grasses. Here, we provide an adapter ligation PCR protocol that allows the large-scale characterization of T-DNA insertions into the genome of Brachypodium. The procedure enables the retrieval and mapping of the regions flanking the right and left borders (RB and LB) of the T-DNA inserts and consists of five steps: extraction and restriction digest of genomic DNA; ligation of an adapter to the genomic DNA; PCR amplification of the regions flanking the T-DNA insert(s) using primers specific to the adapter and the T-DNA; sequencing of the PCR products; and identification of the flanking sequence tags (FSTs) characterizing the T-DNA inserts. Analyzing the regions flanking both the LB and RB of the T-DNA inserts significantly improves FST retrieval and the frequency of mutant lines for which at least one FST can be identified. It takes approximately 16 or 10 d for a single person to analyze 96 T-DNA lines using individual or batch procedures, respectively. More... »

PAGES

650-661

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2009.32

DOI

http://dx.doi.org/10.1038/nprot.2009.32

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031277727

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19360020


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Insertional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Poaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Tagged Sites", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thole", 
        "givenName": "Vera", 
        "id": "sg:person.01047625321.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047625321.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alves", 
        "givenName": "S\u00edlvia C", 
        "id": "sg:person.01074312514.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074312514.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Worland", 
        "givenName": "Barbara", 
        "id": "sg:person.01142425714.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142425714.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bevan", 
        "givenName": "Michael W", 
        "id": "sg:person.010003027062.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010003027062.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vain", 
        "givenName": "Philippe", 
        "id": "sg:person.01115740521.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115740521.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrg2286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003762064", 
          "https://doi.org/10.1038/nrg2286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00122-003-1255-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050258094", 
          "https://doi.org/10.1007/s00122-003-1255-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00122-002-1184-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034890783", 
          "https://doi.org/10.1007/s00122-002-1184-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2007.425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025302814", 
          "https://doi.org/10.1038/nprot.2007.425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-003-0704-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008422520", 
          "https://doi.org/10.1007/s00299-003-0704-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:plan.0000036368.74758.66", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006961221", 
          "https://doi.org/10.1023/b:plan.0000036368.74758.66"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00122-004-1692-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000898161", 
          "https://doi.org/10.1007/s00122-004-1692-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032411227", 
          "https://doi.org/10.1038/nbt.1491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011475446", 
          "https://doi.org/10.1038/nature04434"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-04-09", 
    "datePublishedReg": "2009-04-09", 
    "description": "Brachypodium distachyon is emerging as a new model system for bridging research into temperate cereal crops, such as wheat and barley, and for promoting research in novel biomass grasses. Here, we provide an adapter ligation PCR protocol that allows the large-scale characterization of T-DNA insertions into the genome of Brachypodium. The procedure enables the retrieval and mapping of the regions flanking the right and left borders (RB and LB) of the T-DNA inserts and consists of five steps: extraction and restriction digest of genomic DNA; ligation of an adapter to the genomic DNA; PCR amplification of the regions flanking the T-DNA insert(s) using primers specific to the adapter and the T-DNA; sequencing of the PCR products; and identification of the flanking sequence tags (FSTs) characterizing the T-DNA inserts. Analyzing the regions flanking both the LB and RB of the T-DNA inserts significantly improves FST retrieval and the frequency of mutant lines for which at least one FST can be identified. It takes approximately 16 or 10 d for a single person to analyze 96 T-DNA lines using individual or batch procedures, respectively.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nprot.2009.32", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1037502", 
        "issn": [
          "1754-2189", 
          "1750-2799"
        ], 
        "name": "Nature Protocols", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "4"
      }
    ], 
    "keywords": [
      "T-DNA inserts", 
      "T-DNA", 
      "sequence tags", 
      "T-DNA insertional mutants", 
      "genomic DNA", 
      "T-DNA insertion", 
      "temperate cereal crops", 
      "T-DNA lines", 
      "large-scale characterization", 
      "new model system", 
      "Brachypodium distachyon", 
      "insertional mutants", 
      "mutant lines", 
      "cereal crops", 
      "restriction digests", 
      "PCR amplification", 
      "model system", 
      "PCR products", 
      "PCR protocol", 
      "DNA", 
      "Brachypodium", 
      "distachyon", 
      "tags", 
      "genome", 
      "mutants", 
      "inserts", 
      "sequencing", 
      "wheat", 
      "barley", 
      "grass", 
      "crops", 
      "FST", 
      "adapter", 
      "primers", 
      "region", 
      "lines", 
      "digests", 
      "amplification", 
      "identification", 
      "insertion", 
      "characterization", 
      "mapping", 
      "Rb", 
      "ligation", 
      "step", 
      "products", 
      "protocol", 
      "lb", 
      "border", 
      "batch procedure", 
      "research", 
      "system", 
      "frequency", 
      "extraction", 
      "procedure", 
      "retrieval", 
      "retrieving", 
      "single person", 
      "rights", 
      "persons"
    ], 
    "name": "A protocol for efficiently retrieving and characterizing flanking sequence tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants", 
    "pagination": "650-661", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031277727"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nprot.2009.32"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19360020"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nprot.2009.32", 
      "https://app.dimensions.ai/details/publication/pub.1031277727"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T20:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_482.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nprot.2009.32"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2009.32'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2009.32'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2009.32'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2009.32'


 

This table displays all metadata directly associated to this object as RDF triples.

231 TRIPLES      21 PREDICATES      105 URIs      88 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nprot.2009.32 schema:about N03f2de7061cb43a8b92ef53ac3d64eef
2 N04d9bf57f8a94a9396f735c17ad8494d
3 N09276916c09f4457ad79dea02ee53095
4 N09c266ba80a343c8987cb16c60a6f70a
5 N0b0cfaf213f148e1b08810ba750f6fc8
6 N1d0e2376d8b249ddb6ca39569bdac630
7 N2c05b0e741d84a77b54c6f0f37655e0f
8 N617a09e8c4f04d3aa9fb844a2ce7ee98
9 N86120a1aa3f24905b7c1d990ded775d0
10 Na90bd7a0a71a403ebc965ade243376fb
11 Nfe88f6bcf0e64ec2b7f52acf633eaba5
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author Nb58999b48b834f6b9532ed7b6972c83c
15 schema:citation sg:pub.10.1007/s00122-002-1184-x
16 sg:pub.10.1007/s00122-003-1255-7
17 sg:pub.10.1007/s00122-004-1692-y
18 sg:pub.10.1007/s00299-003-0704-8
19 sg:pub.10.1023/b:plan.0000036368.74758.66
20 sg:pub.10.1038/nature04434
21 sg:pub.10.1038/nbt.1491
22 sg:pub.10.1038/nprot.2007.425
23 sg:pub.10.1038/nrg2286
24 schema:datePublished 2009-04-09
25 schema:datePublishedReg 2009-04-09
26 schema:description Brachypodium distachyon is emerging as a new model system for bridging research into temperate cereal crops, such as wheat and barley, and for promoting research in novel biomass grasses. Here, we provide an adapter ligation PCR protocol that allows the large-scale characterization of T-DNA insertions into the genome of Brachypodium. The procedure enables the retrieval and mapping of the regions flanking the right and left borders (RB and LB) of the T-DNA inserts and consists of five steps: extraction and restriction digest of genomic DNA; ligation of an adapter to the genomic DNA; PCR amplification of the regions flanking the T-DNA insert(s) using primers specific to the adapter and the T-DNA; sequencing of the PCR products; and identification of the flanking sequence tags (FSTs) characterizing the T-DNA inserts. Analyzing the regions flanking both the LB and RB of the T-DNA inserts significantly improves FST retrieval and the frequency of mutant lines for which at least one FST can be identified. It takes approximately 16 or 10 d for a single person to analyze 96 T-DNA lines using individual or batch procedures, respectively.
27 schema:genre article
28 schema:isAccessibleForFree false
29 schema:isPartOf N15a44800c2b247b79d9558b0f176e664
30 N7342b0b03033480e8b041f3bc5f28b36
31 sg:journal.1037502
32 schema:keywords Brachypodium
33 Brachypodium distachyon
34 DNA
35 FST
36 PCR amplification
37 PCR products
38 PCR protocol
39 Rb
40 T-DNA
41 T-DNA insertion
42 T-DNA insertional mutants
43 T-DNA inserts
44 T-DNA lines
45 adapter
46 amplification
47 barley
48 batch procedure
49 border
50 cereal crops
51 characterization
52 crops
53 digests
54 distachyon
55 extraction
56 frequency
57 genome
58 genomic DNA
59 grass
60 identification
61 insertion
62 insertional mutants
63 inserts
64 large-scale characterization
65 lb
66 ligation
67 lines
68 mapping
69 model system
70 mutant lines
71 mutants
72 new model system
73 persons
74 primers
75 procedure
76 products
77 protocol
78 region
79 research
80 restriction digests
81 retrieval
82 retrieving
83 rights
84 sequence tags
85 sequencing
86 single person
87 step
88 system
89 tags
90 temperate cereal crops
91 wheat
92 schema:name A protocol for efficiently retrieving and characterizing flanking sequence tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants
93 schema:pagination 650-661
94 schema:productId N7cc97475f7bc4319a35be49646a1f1ce
95 N89d0f73c382942019270f1b518d26c2e
96 Nbc14626e3f2d4160b3edc701727ea2be
97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031277727
98 https://doi.org/10.1038/nprot.2009.32
99 schema:sdDatePublished 2022-11-24T20:53
100 schema:sdLicense https://scigraph.springernature.com/explorer/license/
101 schema:sdPublisher N7c9c470a97bd4813919bee02b5dc10d2
102 schema:url https://doi.org/10.1038/nprot.2009.32
103 sgo:license sg:explorer/license/
104 sgo:sdDataset articles
105 rdf:type schema:ScholarlyArticle
106 N03f2de7061cb43a8b92ef53ac3d64eef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Sequence Analysis, DNA
108 rdf:type schema:DefinedTerm
109 N04d9bf57f8a94a9396f735c17ad8494d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Sequence Tagged Sites
111 rdf:type schema:DefinedTerm
112 N068c744230bf40d284c98994d549e1b1 rdf:first sg:person.01115740521.23
113 rdf:rest rdf:nil
114 N09276916c09f4457ad79dea02ee53095 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Molecular Sequence Data
116 rdf:type schema:DefinedTerm
117 N09c266ba80a343c8987cb16c60a6f70a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Mutagenesis, Insertional
119 rdf:type schema:DefinedTerm
120 N0b0cfaf213f148e1b08810ba750f6fc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Genome, Plant
122 rdf:type schema:DefinedTerm
123 N15a44800c2b247b79d9558b0f176e664 schema:volumeNumber 4
124 rdf:type schema:PublicationVolume
125 N1d0e2376d8b249ddb6ca39569bdac630 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name DNA, Bacterial
127 rdf:type schema:DefinedTerm
128 N2c05b0e741d84a77b54c6f0f37655e0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Genetic Vectors
130 rdf:type schema:DefinedTerm
131 N3187d85156e04978b41d0946c4728011 rdf:first sg:person.010003027062.96
132 rdf:rest N068c744230bf40d284c98994d549e1b1
133 N617a09e8c4f04d3aa9fb844a2ce7ee98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Polymerase Chain Reaction
135 rdf:type schema:DefinedTerm
136 N7342b0b03033480e8b041f3bc5f28b36 schema:issueNumber 5
137 rdf:type schema:PublicationIssue
138 N7c9c470a97bd4813919bee02b5dc10d2 schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 N7cc97475f7bc4319a35be49646a1f1ce schema:name dimensions_id
141 schema:value pub.1031277727
142 rdf:type schema:PropertyValue
143 N86120a1aa3f24905b7c1d990ded775d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Poaceae
145 rdf:type schema:DefinedTerm
146 N87aa16b24c684a6081fe536e0d3671ca rdf:first sg:person.01074312514.00
147 rdf:rest Nde12bbc99bce4141b4e16529a8ae8ad4
148 N89d0f73c382942019270f1b518d26c2e schema:name pubmed_id
149 schema:value 19360020
150 rdf:type schema:PropertyValue
151 Na90bd7a0a71a403ebc965ade243376fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name DNA, Plant
153 rdf:type schema:DefinedTerm
154 Nb58999b48b834f6b9532ed7b6972c83c rdf:first sg:person.01047625321.69
155 rdf:rest N87aa16b24c684a6081fe536e0d3671ca
156 Nbc14626e3f2d4160b3edc701727ea2be schema:name doi
157 schema:value 10.1038/nprot.2009.32
158 rdf:type schema:PropertyValue
159 Nde12bbc99bce4141b4e16529a8ae8ad4 rdf:first sg:person.01142425714.72
160 rdf:rest N3187d85156e04978b41d0946c4728011
161 Nfe88f6bcf0e64ec2b7f52acf633eaba5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Base Sequence
163 rdf:type schema:DefinedTerm
164 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
165 schema:name Biological Sciences
166 rdf:type schema:DefinedTerm
167 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
168 schema:name Genetics
169 rdf:type schema:DefinedTerm
170 sg:journal.1037502 schema:issn 1750-2799
171 1754-2189
172 schema:name Nature Protocols
173 schema:publisher Springer Nature
174 rdf:type schema:Periodical
175 sg:person.010003027062.96 schema:affiliation grid-institutes:grid.14830.3e
176 schema:familyName Bevan
177 schema:givenName Michael W
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010003027062.96
179 rdf:type schema:Person
180 sg:person.01047625321.69 schema:affiliation grid-institutes:grid.14830.3e
181 schema:familyName Thole
182 schema:givenName Vera
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047625321.69
184 rdf:type schema:Person
185 sg:person.01074312514.00 schema:affiliation grid-institutes:grid.14830.3e
186 schema:familyName Alves
187 schema:givenName Sílvia C
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074312514.00
189 rdf:type schema:Person
190 sg:person.01115740521.23 schema:affiliation grid-institutes:grid.14830.3e
191 schema:familyName Vain
192 schema:givenName Philippe
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115740521.23
194 rdf:type schema:Person
195 sg:person.01142425714.72 schema:affiliation grid-institutes:grid.14830.3e
196 schema:familyName Worland
197 schema:givenName Barbara
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142425714.72
199 rdf:type schema:Person
200 sg:pub.10.1007/s00122-002-1184-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034890783
201 https://doi.org/10.1007/s00122-002-1184-x
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/s00122-003-1255-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050258094
204 https://doi.org/10.1007/s00122-003-1255-7
205 rdf:type schema:CreativeWork
206 sg:pub.10.1007/s00122-004-1692-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1000898161
207 https://doi.org/10.1007/s00122-004-1692-y
208 rdf:type schema:CreativeWork
209 sg:pub.10.1007/s00299-003-0704-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008422520
210 https://doi.org/10.1007/s00299-003-0704-8
211 rdf:type schema:CreativeWork
212 sg:pub.10.1023/b:plan.0000036368.74758.66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006961221
213 https://doi.org/10.1023/b:plan.0000036368.74758.66
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/nature04434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011475446
216 https://doi.org/10.1038/nature04434
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nbt.1491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032411227
219 https://doi.org/10.1038/nbt.1491
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nprot.2007.425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025302814
222 https://doi.org/10.1038/nprot.2007.425
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nrg2286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003762064
225 https://doi.org/10.1038/nrg2286
226 rdf:type schema:CreativeWork
227 grid-institutes:grid.14830.3e schema:alternateName Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK
228 Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK
229 schema:name Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK
230 Department of Crop Genetics, John Innes Centre, Norwich Research Park, NR4 7UH, Colney Lane, Norwich, UK
231 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...