A rapid HPLC-MS/MS method for the simultaneous quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human blood samples View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-04

AUTHORS

Christoph Seger, Karin Tentschert, Wolfgang Stöggl, Andrea Griesmacher, Steven L Ramsay

ABSTRACT

In the past few years, high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has matured to a true alternative to antibody-based immunoassays in routine therapeutic drug monitoring. In transplantation medicine, mass spectrometry-based assessment of immunosuppressant drug levels is considered a gold standard diagnostic procedure. We describe a fast state-of-the-art routine online solid-phase extraction (SPE) HPLC-MS/MS analysis platform that allows monitoring of cyclosporine A, tacrolimus, sirolimus and everolimus from 50-microl aliquots of EDTA whole blood specimens within 3.4 min total analysis time. Sample purification is done by offline protein precipitation followed by two automated chromatographic separation steps. Mass spectrometry-based analyte quantification relies on selected reaction monitoring experiments. The assay underwent complete validation and performance evaluation studies and performs very well in several international proficiency testing schemes. In daily routine, it allows reporting of about 75 patient sample results per work shift with a typical total individual sample turnaround time of less than 3 h. More... »

PAGES

526-534

References to SciGraph publications

  • 2007-08. Current role of LC-MS in therapeutic drug monitoring in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2009.25

    DOI

    http://dx.doi.org/10.1038/nprot.2009.25

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19325549


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