Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics View Full Text


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Article Info

DATE

2009-03-19

AUTHORS

Paul J Boersema, Reinout Raijmakers, Simone Lemeer, Shabaz Mohammed, Albert J R Heck

ABSTRACT

Accurate quantification of protein expression in biological systems is an increasingly important part of proteomics research. Incorporation of differential stable isotopes in samples for relative protein quantification has been widely used. Stable isotope incorporation at the peptide level using dimethyl labeling is a reliable, cost-effective and undemanding procedure that can be easily automated and applied in high-throughput proteomics experiments. Although alternative multiplex quantitative proteomics approaches introduce isotope labels at the organism level ('stable isotope labeling by amino acids in cell culture' (SILAC)) or enable the simultaneous analysis of eight samples (isobaric tagging for relative and absolute quantification (iTRAQ)), stable isotope dimethyl labeling is advantageous in that it uses inexpensive reagents and is applicable to virtually any sample. We describe in-solution, online and on-column protocols for stable isotope dimethyl labeling of sample amounts ranging from sub-micrograms to milligrams. The labeling steps take approximately 60–90 min, whereas the full protocol including digestion and (two-dimensional) liquid chromatography-mass spectrometry takes approximately 1.5–3 days to complete. More... »

PAGES

484-494

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2009.21

DOI

http://dx.doi.org/10.1038/nprot.2009.21

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049644591

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19300442


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