Analyzing real-time PCR data by the comparative CT method View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-06

AUTHORS

Thomas D Schmittgen, Kenneth J Livak

ABSTRACT

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method. More... »

PAGES

1101-1108

Journal

TITLE

Nature Protocols

ISSUE

6

VOLUME

3

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2008.73

    DOI

    http://dx.doi.org/10.1038/nprot.2008.73

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1005355981

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18546601


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