A protocol for determination of anticardiolipin antibodies by ELISA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-04-17

AUTHORS

Silvia S Pierangeli, Eon Nigel Harris

ABSTRACT

The anticardiolipin (aCL) test has been widely used by physicians since the mid-1980s for diagnosing patients with antiphospholipid syndrome (APS). Establishment of this diagnosis has enabled effective management of patients with recurrent thrombosis or recurrent pregnancy losses. The test was first established in 1983 as a radioimmunoassay and soon thereafter converted into ELISA. There have been numerous efforts to standardize the aCL test, but precise reproducible measurement of aCL levels is difficult and the use of semiquantitative measurements (high, medium and low) is recommended as this is probably sufficient for clinical diagnosis. Using validated ELISAs for measuring aCL Abs offers greater reproducibility, would reduce interlaboratory variations and limit discrepancies in results between different laboratories. This article details a procedure that takes ∼2 h and summarizes the information available on the aCL ELISA test. More... »

PAGES

840-848

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2008.48

DOI

http://dx.doi.org/10.1038/nprot.2008.48

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027654665

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18451792


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1117", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Public Health and Health Services", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Anticardiolipin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antiphospholipid Syndrome", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme-Linked Immunosorbent Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sensitivity and Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, USA", 
          "id": "http://www.grid.ac/institutes/grid.176731.5", 
          "name": [
            "Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pierangeli", 
        "givenName": "Silvia S", 
        "id": "sg:person.01026125740.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026125740.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Office of the Vice Chancellor, University of the West Indies, Kingston, JamaicaWest Indies", 
          "id": "http://www.grid.ac/institutes/grid.12916.3d", 
          "name": [
            "Office of the Vice Chancellor, University of the West Indies, Kingston, JamaicaWest Indies"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harris", 
        "givenName": "Eon Nigel", 
        "id": "sg:person.011340100772.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011340100772.08"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00917565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021467486", 
          "https://doi.org/10.1007/bf00917565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02031064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047081919", 
          "https://doi.org/10.1007/bf02031064"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-04-17", 
    "datePublishedReg": "2008-04-17", 
    "description": "The anticardiolipin (aCL) test has been widely used by physicians since the mid-1980s for diagnosing patients with antiphospholipid syndrome (APS). Establishment of this diagnosis has enabled effective management of patients with recurrent thrombosis or recurrent pregnancy losses. The test was first established in 1983 as a radioimmunoassay and soon thereafter converted into ELISA. There have been numerous efforts to standardize the aCL test, but precise reproducible measurement of aCL levels is difficult and the use of semiquantitative measurements (high, medium and low) is recommended as this is probably sufficient for clinical diagnosis. Using validated ELISAs for measuring aCL Abs offers greater reproducibility, would reduce interlaboratory variations and limit discrepancies in results between different laboratories. This article details a procedure that takes \u223c2 h and summarizes the information available on the aCL ELISA test.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nprot.2008.48", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1037502", 
        "issn": [
          "1754-2189", 
          "1750-2799"
        ], 
        "name": "Nature Protocols", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "antiphospholipid syndrome", 
      "recurrent pregnancy loss", 
      "aCL Abs", 
      "aCL levels", 
      "anticardiolipin antibodies", 
      "recurrent thrombosis", 
      "pregnancy loss", 
      "anticardiolipin tests", 
      "clinical diagnosis", 
      "ELISA test", 
      "aCL test", 
      "semiquantitative measurement", 
      "ELISA", 
      "patients", 
      "diagnosis", 
      "interlaboratory variation", 
      "reproducible measurements", 
      "thrombosis", 
      "syndrome", 
      "physicians", 
      "radioimmunoassay", 
      "antibodies", 
      "effective management", 
      "test", 
      "different laboratories", 
      "greater reproducibility", 
      "Ab", 
      "management", 
      "levels", 
      "protocol", 
      "numerous efforts", 
      "loss", 
      "procedure", 
      "use", 
      "laboratory", 
      "reproducibility", 
      "discrepancy", 
      "measurements", 
      "results", 
      "establishment", 
      "efforts", 
      "information", 
      "article", 
      "determination", 
      "variation"
    ], 
    "name": "A protocol for determination of anticardiolipin antibodies by ELISA", 
    "pagination": "840-848", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027654665"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nprot.2008.48"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18451792"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nprot.2008.48", 
      "https://app.dimensions.ai/details/publication/pub.1027654665"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-10T09:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_469.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nprot.2008.48"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.48'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.48'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.48'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.48'


 

This table displays all metadata directly associated to this object as RDF triples.

145 TRIPLES      22 PREDICATES      77 URIs      67 LITERALS      12 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nprot.2008.48 schema:about N0762bfae302c4059934b660791ad8c08
2 N0b59dea0c478409791bfd24fc8ff6e48
3 N62b9aed1195b489caaad68eb6fe08cfd
4 N7497600c6d684ebb9ee128c40e853009
5 N78ed265cd83241ce833d41202f780dc1
6 anzsrc-for:11
7 anzsrc-for:1117
8 schema:author N0975eb59d9094d669c620057e7d4543a
9 schema:citation sg:pub.10.1007/bf00917565
10 sg:pub.10.1007/bf02031064
11 schema:datePublished 2008-04-17
12 schema:datePublishedReg 2008-04-17
13 schema:description The anticardiolipin (aCL) test has been widely used by physicians since the mid-1980s for diagnosing patients with antiphospholipid syndrome (APS). Establishment of this diagnosis has enabled effective management of patients with recurrent thrombosis or recurrent pregnancy losses. The test was first established in 1983 as a radioimmunoassay and soon thereafter converted into ELISA. There have been numerous efforts to standardize the aCL test, but precise reproducible measurement of aCL levels is difficult and the use of semiquantitative measurements (high, medium and low) is recommended as this is probably sufficient for clinical diagnosis. Using validated ELISAs for measuring aCL Abs offers greater reproducibility, would reduce interlaboratory variations and limit discrepancies in results between different laboratories. This article details a procedure that takes ∼2 h and summarizes the information available on the aCL ELISA test.
14 schema:genre article
15 schema:inLanguage en
16 schema:isAccessibleForFree false
17 schema:isPartOf N1017c6a7451143a69e73f6cf0f2440b1
18 Nd40d3d0c813d41b59a941e8e82004553
19 sg:journal.1037502
20 schema:keywords Ab
21 ELISA
22 ELISA test
23 aCL Abs
24 aCL levels
25 aCL test
26 antibodies
27 anticardiolipin antibodies
28 anticardiolipin tests
29 antiphospholipid syndrome
30 article
31 clinical diagnosis
32 determination
33 diagnosis
34 different laboratories
35 discrepancy
36 effective management
37 efforts
38 establishment
39 greater reproducibility
40 information
41 interlaboratory variation
42 laboratory
43 levels
44 loss
45 management
46 measurements
47 numerous efforts
48 patients
49 physicians
50 pregnancy loss
51 procedure
52 protocol
53 radioimmunoassay
54 recurrent pregnancy loss
55 recurrent thrombosis
56 reproducibility
57 reproducible measurements
58 results
59 semiquantitative measurement
60 syndrome
61 test
62 thrombosis
63 use
64 variation
65 schema:name A protocol for determination of anticardiolipin antibodies by ELISA
66 schema:pagination 840-848
67 schema:productId N480b21de2f054b3eb0867e095c029bfd
68 N8e9eb2a2a7154380a30710e84db71590
69 Nb2ed9bf5984648b387f033b3b0fcafe4
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027654665
71 https://doi.org/10.1038/nprot.2008.48
72 schema:sdDatePublished 2022-05-10T09:55
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N1dca055b68884465981f53a62d7d23ae
75 schema:url https://doi.org/10.1038/nprot.2008.48
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N0762bfae302c4059934b660791ad8c08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Humans
81 rdf:type schema:DefinedTerm
82 N0975eb59d9094d669c620057e7d4543a rdf:first sg:person.01026125740.15
83 rdf:rest N6b546ec1e5cd4ebb9435c16b8e8da50a
84 N0b59dea0c478409791bfd24fc8ff6e48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Antibodies, Anticardiolipin
86 rdf:type schema:DefinedTerm
87 N1017c6a7451143a69e73f6cf0f2440b1 schema:issueNumber 5
88 rdf:type schema:PublicationIssue
89 N1dca055b68884465981f53a62d7d23ae schema:name Springer Nature - SN SciGraph project
90 rdf:type schema:Organization
91 N480b21de2f054b3eb0867e095c029bfd schema:name pubmed_id
92 schema:value 18451792
93 rdf:type schema:PropertyValue
94 N62b9aed1195b489caaad68eb6fe08cfd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Sensitivity and Specificity
96 rdf:type schema:DefinedTerm
97 N6b546ec1e5cd4ebb9435c16b8e8da50a rdf:first sg:person.011340100772.08
98 rdf:rest rdf:nil
99 N7497600c6d684ebb9ee128c40e853009 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Antiphospholipid Syndrome
101 rdf:type schema:DefinedTerm
102 N78ed265cd83241ce833d41202f780dc1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Enzyme-Linked Immunosorbent Assay
104 rdf:type schema:DefinedTerm
105 N8e9eb2a2a7154380a30710e84db71590 schema:name dimensions_id
106 schema:value pub.1027654665
107 rdf:type schema:PropertyValue
108 Nb2ed9bf5984648b387f033b3b0fcafe4 schema:name doi
109 schema:value 10.1038/nprot.2008.48
110 rdf:type schema:PropertyValue
111 Nd40d3d0c813d41b59a941e8e82004553 schema:volumeNumber 3
112 rdf:type schema:PublicationVolume
113 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
114 schema:name Medical and Health Sciences
115 rdf:type schema:DefinedTerm
116 anzsrc-for:1117 schema:inDefinedTermSet anzsrc-for:
117 schema:name Public Health and Health Services
118 rdf:type schema:DefinedTerm
119 sg:journal.1037502 schema:issn 1750-2799
120 1754-2189
121 schema:name Nature Protocols
122 schema:publisher Springer Nature
123 rdf:type schema:Periodical
124 sg:person.01026125740.15 schema:affiliation grid-institutes:grid.176731.5
125 schema:familyName Pierangeli
126 schema:givenName Silvia S
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026125740.15
128 rdf:type schema:Person
129 sg:person.011340100772.08 schema:affiliation grid-institutes:grid.12916.3d
130 schema:familyName Harris
131 schema:givenName Eon Nigel
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011340100772.08
133 rdf:type schema:Person
134 sg:pub.10.1007/bf00917565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021467486
135 https://doi.org/10.1007/bf00917565
136 rdf:type schema:CreativeWork
137 sg:pub.10.1007/bf02031064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047081919
138 https://doi.org/10.1007/bf02031064
139 rdf:type schema:CreativeWork
140 grid-institutes:grid.12916.3d schema:alternateName Office of the Vice Chancellor, University of the West Indies, Kingston, JamaicaWest Indies
141 schema:name Office of the Vice Chancellor, University of the West Indies, Kingston, JamaicaWest Indies
142 rdf:type schema:Organization
143 grid-institutes:grid.176731.5 schema:alternateName Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, USA
144 schema:name Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, USA
145 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...