Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed View Full Text


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Article Info

DATE

2008-05

AUTHORS

Yukoh Hiei, Toshihiko Komari

ABSTRACT

Here, we provide comprehensive, highly efficient protocols for Agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes. Methods that use either immature embryos (japonica and indica rice) or calli (japonica cultivars and the indica cultivar, Kasalath) as a starting material for inoculation with Agrobacterium are described. Immature embryos are pretreated with heat and centrifugal force, which significantly enhances the efficiency of gene transfer, and then infected with Agrobacterium. Callus is induced from mature seeds and infected. Transformed cells proliferated from these tissues are selected on the basis of hygromycin resistance, and transgenic plants are eventually regenerated. A single immature japonica or Kasalath embryo will produce between 10 and 18 independent transgenic plants; for other non-Kasalath indica varieties, the number of transgenic plants expected will be between 5 and 13. For japonica and Kasalath, transformants should be obtained from between 50 and 90% of calli. From inoculation with Agrobacterium to transplanting to soil will take 55 d for japonica and Kasalath, and 74 d for indica other than Kasalath using the immature embryo method, and 50 d for japonica and Kasalath using the callus method. More... »

PAGES

824-834

References to SciGraph publications

  • 2006-06. Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2003-12. Improvement of rice transformation using bombardment of scutellum-derived calli in PLANT MOLECULAR BIOLOGY REPORTER
  • 1991-10. Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos in NATURE BIOTECHNOLOGY
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  • 2005-03. An improvedAgrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars in PLANT MOLECULAR BIOLOGY REPORTER
  • 2001-01. Effective selection and regeneration of transgenic rice plants with mannose as selective agent in MOLECULAR BREEDING
  • 1999-03. Relationships between nitrite reductase activity and genotype-dependent callus growth in rice cell cultures in PLANT CELL REPORTS
  • 1999-12. Agrobacterium-mediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2003-03. Histone H4 Acetylation Patterns During Seed Germination and Early Plant Development in BIOLOGIA PLANTARUM
  • 2003-03. High-frequencyAgrobacterium-med’iated genetic transformation of tongil rice varieties in JOURNAL OF PLANT BIOLOGY
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  • 1987-05. Isozymes and classification of Asian rice varieties in THEORETICAL AND APPLIED GENETICS
  • 2006-06-01. Japonica Rice Varieties (<i>Oryza sativa</i>, Nipponbare, and Others) in AGROBACTERIUM PROTOCOLS
  • 2005-01. Optimising the tissue culture conditions for high efficiency transformation of indica rice in PLANT CELL REPORTS
  • 2006-12. Improved frequency of transformation in rice and maize by treatment of immature embryos with centrifugation and heat prior to infection with Agrobacterium tumefaciens in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2001-06. Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer in CELL RESEARCH
  • 2005-07. Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-03. Fertile transgenic rice plants regenerated from transformed protoplasts in NATURE
  • 1988-09. Transgenic Rice Plants After Direct Gene Transfer into Protoplasts in NATURE BIOTECHNOLOGY
  • 2004-03. Transformation of Indica Rice Through Particle Bombardment: Factors Influencing Transient Expression and Selection in BIOLOGIA PLANTARUM
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2008.46

    DOI

    http://dx.doi.org/10.1038/nprot.2008.46

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18451790


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