Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-05

AUTHORS

Yukoh Hiei, Toshihiko Komari

ABSTRACT

Here, we provide comprehensive, highly efficient protocols for Agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes. Methods that use either immature embryos (japonica and indica rice) or calli (japonica cultivars and the indica cultivar, Kasalath) as a starting material for inoculation with Agrobacterium are described. Immature embryos are pretreated with heat and centrifugal force, which significantly enhances the efficiency of gene transfer, and then infected with Agrobacterium. Callus is induced from mature seeds and infected. Transformed cells proliferated from these tissues are selected on the basis of hygromycin resistance, and transgenic plants are eventually regenerated. A single immature japonica or Kasalath embryo will produce between 10 and 18 independent transgenic plants; for other non-Kasalath indica varieties, the number of transgenic plants expected will be between 5 and 13. For japonica and Kasalath, transformants should be obtained from between 50 and 90% of calli. From inoculation with Agrobacterium to transplanting to soil will take 55 d for japonica and Kasalath, and 74 d for indica other than Kasalath using the immature embryo method, and 50 d for japonica and Kasalath using the callus method. More... »

PAGES

824-834

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2008.46

DOI

http://dx.doi.org/10.1038/nprot.2008.46

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045873077

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18451790


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Agrobacterium tumefaciens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oryza", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Seeds", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Japan Tobacco (Japan)", 
          "id": "https://www.grid.ac/institutes/grid.417743.2", 
          "name": [
            "Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hiei", 
        "givenName": "Yukoh", 
        "id": "sg:person.01041560021.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041560021.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Tobacco (Japan)", 
          "id": "https://www.grid.ac/institutes/grid.417743.2", 
          "name": [
            "Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Komari", 
        "givenName": "Toshihiko", 
        "id": "sg:person.01207707662.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207707662.42"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01977351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235724", 
          "https://doi.org/10.1007/bf01977351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01977351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235724", 
          "https://doi.org/10.1007/bf01977351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1009661014167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004195512", 
          "https://doi.org/10.1023/a:1009661014167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11240-005-9069-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007858377", 
          "https://doi.org/10.1007/s11240-005-9069-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb.68.1193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007861462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0890-736", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010038336", 
          "https://doi.org/10.1038/nbt0890-736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02772648", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010639497", 
          "https://doi.org/10.1007/bf02772648"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11240-006-9157-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013520828", 
          "https://doi.org/10.1007/s11240-006-9157-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(85)90086-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013553608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1091-957", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014438272", 
          "https://doi.org/10.1038/nbt1091-957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006307527449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019437708", 
          "https://doi.org/10.1023/a:1006307527449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11240-004-6154-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022186587", 
          "https://doi.org/10.1007/s11240-004-6154-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1994.6020271.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023112743"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1022393629691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024311747", 
          "https://doi.org/10.1023/a:1022393629691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026558025", 
          "https://doi.org/10.1038/sj.cr.149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026558025", 
          "https://doi.org/10.1038/sj.cr.149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(68)90403-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027478679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.71.9.3672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028573273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/1-59745-130-4:213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032232480", 
          "https://doi.org/10.1385/1-59745-130-4:213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.plantsci.2005.09.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032445032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.plantsci.2005.09.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032445032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0988-1072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032752526", 
          "https://doi.org/10.1038/nbt0988-1072"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0884-702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033876966", 
          "https://doi.org/10.1038/nbt0884-702"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267709", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035059284", 
          "https://doi.org/10.1007/bf00267709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267709", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035059284", 
          "https://doi.org/10.1007/bf00267709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-004-0843-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037588278", 
          "https://doi.org/10.1007/s00299-004-0843-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-004-0843-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037588278", 
          "https://doi.org/10.1007/s00299-004-0843-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-004-0843-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037588278", 
          "https://doi.org/10.1007/s00299-004-0843-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/338274a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040743981", 
          "https://doi.org/10.1038/338274a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/338274a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040743981", 
          "https://doi.org/10.1038/338274a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.2006.02836.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040941373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002990050625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042646539", 
          "https://doi.org/10.1007/s002990050625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002990050625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042646539", 
          "https://doi.org/10.1007/s002990050625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf03030297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044288803", 
          "https://doi.org/10.1007/bf03030297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02772592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048222118", 
          "https://doi.org/10.1007/bf02772592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:biop.0000024271.38723.a6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049281360", 
          "https://doi.org/10.1023/b:biop.0000024271.38723.a6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00290078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051467224", 
          "https://doi.org/10.1007/bf00290078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.166.1.88-94.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062714858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077299600", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077299601", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.pcp.a077982", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083493326"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-05", 
    "datePublishedReg": "2008-05-01", 
    "description": "Here, we provide comprehensive, highly efficient protocols for Agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes. Methods that use either immature embryos (japonica and indica rice) or calli (japonica cultivars and the indica cultivar, Kasalath) as a starting material for inoculation with Agrobacterium are described. Immature embryos are pretreated with heat and centrifugal force, which significantly enhances the efficiency of gene transfer, and then infected with Agrobacterium. Callus is induced from mature seeds and infected. Transformed cells proliferated from these tissues are selected on the basis of hygromycin resistance, and transgenic plants are eventually regenerated. A single immature japonica or Kasalath embryo will produce between 10 and 18 independent transgenic plants; for other non-Kasalath indica varieties, the number of transgenic plants expected will be between 5 and 13. For japonica and Kasalath, transformants should be obtained from between 50 and 90% of calli. From inoculation with Agrobacterium to transplanting to soil will take 55 d for japonica and Kasalath, and 74 d for indica other than Kasalath using the immature embryo method, and 50 d for japonica and Kasalath using the callus method.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nprot.2008.46", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1037502", 
        "issn": [
          "1754-2189", 
          "1750-2799"
        ], 
        "name": "Nature Protocols", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "name": "Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed", 
    "pagination": "824-834", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "da8377b75979af5ecb306590b4c02bae9e110738251cfa329930e8283ff10b17"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18451790"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101284307"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nprot.2008.46"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045873077"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nprot.2008.46", 
      "https://app.dimensions.ai/details/publication/pub.1045873077"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/nprot.2008.46"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.46'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.46'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.46'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nprot.2008.46'


 

This table displays all metadata directly associated to this object as RDF triples.

226 TRIPLES      21 PREDICATES      70 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nprot.2008.46 schema:about N0e6e41d4e11f4e0280bfc8a65c151ab6
2 N23651d802ec548779bfeecb0ec43a948
3 N5789cac02b35490e809c3bde2fb538f6
4 N59c5cfb8e16c463b8cff28ff738fed50
5 N643ce1d548b042a8804bc33a8cbed34e
6 Nf398cd86f5214c19a8623dc4b91004f7
7 Nf7a510447db74693b42c007ccd33c42d
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author N8904792b4d994813912b29aa21eb612e
11 schema:citation sg:pub.10.1007/bf00267709
12 sg:pub.10.1007/bf00290078
13 sg:pub.10.1007/bf01977351
14 sg:pub.10.1007/bf02772592
15 sg:pub.10.1007/bf02772648
16 sg:pub.10.1007/bf03030297
17 sg:pub.10.1007/s00299-004-0843-6
18 sg:pub.10.1007/s002990050625
19 sg:pub.10.1007/s11240-004-6154-3
20 sg:pub.10.1007/s11240-005-9069-8
21 sg:pub.10.1007/s11240-006-9157-4
22 sg:pub.10.1023/a:1006307527449
23 sg:pub.10.1023/a:1009661014167
24 sg:pub.10.1023/a:1022393629691
25 sg:pub.10.1023/b:biop.0000024271.38723.a6
26 sg:pub.10.1038/338274a0
27 sg:pub.10.1038/nbt0884-702
28 sg:pub.10.1038/nbt0890-736
29 sg:pub.10.1038/nbt0988-1072
30 sg:pub.10.1038/nbt1091-957
31 sg:pub.10.1038/sj.cr.149
32 sg:pub.10.1385/1-59745-130-4:213
33 https://app.dimensions.ai/details/publication/pub.1077299600
34 https://app.dimensions.ai/details/publication/pub.1077299601
35 https://doi.org/10.1016/0014-4827(68)90403-5
36 https://doi.org/10.1016/0168-9452(85)90086-x
37 https://doi.org/10.1016/j.plantsci.2005.09.015
38 https://doi.org/10.1046/j.1365-313x.1994.6020271.x
39 https://doi.org/10.1073/pnas.71.9.3672
40 https://doi.org/10.1093/oxfordjournals.pcp.a077982
41 https://doi.org/10.1111/j.1365-313x.2006.02836.x
42 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
43 https://doi.org/10.1128/jb.166.1.88-94.1986
44 https://doi.org/10.1271/bbb.68.1193
45 schema:datePublished 2008-05
46 schema:datePublishedReg 2008-05-01
47 schema:description Here, we provide comprehensive, highly efficient protocols for Agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes. Methods that use either immature embryos (japonica and indica rice) or calli (japonica cultivars and the indica cultivar, Kasalath) as a starting material for inoculation with Agrobacterium are described. Immature embryos are pretreated with heat and centrifugal force, which significantly enhances the efficiency of gene transfer, and then infected with Agrobacterium. Callus is induced from mature seeds and infected. Transformed cells proliferated from these tissues are selected on the basis of hygromycin resistance, and transgenic plants are eventually regenerated. A single immature japonica or Kasalath embryo will produce between 10 and 18 independent transgenic plants; for other non-Kasalath indica varieties, the number of transgenic plants expected will be between 5 and 13. For japonica and Kasalath, transformants should be obtained from between 50 and 90% of calli. From inoculation with Agrobacterium to transplanting to soil will take 55 d for japonica and Kasalath, and 74 d for indica other than Kasalath using the immature embryo method, and 50 d for japonica and Kasalath using the callus method.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree false
51 schema:isPartOf Nb3aed5ebe091429ea25e78f5a68007fd
52 Nf4d5eb189bc44199976d7e566a07c632
53 sg:journal.1037502
54 schema:name Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed
55 schema:pagination 824-834
56 schema:productId N18bea06ba9b347bd8a4ea7d8fe969bcf
57 N23d06592e118449cb224239b143ad8a5
58 N69f27571ecde4438af13b0c655af37d6
59 Ne7bf5f0911a142638a3cdee496d6fd0f
60 Ne85d86297b9b410d801da9a8984bcb2b
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045873077
62 https://doi.org/10.1038/nprot.2008.46
63 schema:sdDatePublished 2019-04-10T23:12
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N117c5ef5297c40b693f7add03af0d6b5
66 schema:url http://www.nature.com/articles/nprot.2008.46
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N0e6e41d4e11f4e0280bfc8a65c151ab6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Agrobacterium tumefaciens
72 rdf:type schema:DefinedTerm
73 N117c5ef5297c40b693f7add03af0d6b5 schema:name Springer Nature - SN SciGraph project
74 rdf:type schema:Organization
75 N18bea06ba9b347bd8a4ea7d8fe969bcf schema:name dimensions_id
76 schema:value pub.1045873077
77 rdf:type schema:PropertyValue
78 N23651d802ec548779bfeecb0ec43a948 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Genetic Vectors
80 rdf:type schema:DefinedTerm
81 N23d06592e118449cb224239b143ad8a5 schema:name readcube_id
82 schema:value da8377b75979af5ecb306590b4c02bae9e110738251cfa329930e8283ff10b17
83 rdf:type schema:PropertyValue
84 N3d4c8acf25c24982b5f53eb25dd4d4c2 rdf:first sg:person.01207707662.42
85 rdf:rest rdf:nil
86 N5789cac02b35490e809c3bde2fb538f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Seeds
88 rdf:type schema:DefinedTerm
89 N59c5cfb8e16c463b8cff28ff738fed50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Oryza
91 rdf:type schema:DefinedTerm
92 N643ce1d548b042a8804bc33a8cbed34e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Transformation, Genetic
94 rdf:type schema:DefinedTerm
95 N69f27571ecde4438af13b0c655af37d6 schema:name nlm_unique_id
96 schema:value 101284307
97 rdf:type schema:PropertyValue
98 N8904792b4d994813912b29aa21eb612e rdf:first sg:person.01041560021.04
99 rdf:rest N3d4c8acf25c24982b5f53eb25dd4d4c2
100 Nb3aed5ebe091429ea25e78f5a68007fd schema:issueNumber 5
101 rdf:type schema:PublicationIssue
102 Ne7bf5f0911a142638a3cdee496d6fd0f schema:name doi
103 schema:value 10.1038/nprot.2008.46
104 rdf:type schema:PropertyValue
105 Ne85d86297b9b410d801da9a8984bcb2b schema:name pubmed_id
106 schema:value 18451790
107 rdf:type schema:PropertyValue
108 Nf398cd86f5214c19a8623dc4b91004f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Plants, Genetically Modified
110 rdf:type schema:DefinedTerm
111 Nf4d5eb189bc44199976d7e566a07c632 schema:volumeNumber 3
112 rdf:type schema:PublicationVolume
113 Nf7a510447db74693b42c007ccd33c42d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Gene Transfer Techniques
115 rdf:type schema:DefinedTerm
116 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
117 schema:name Biological Sciences
118 rdf:type schema:DefinedTerm
119 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biochemistry and Cell Biology
121 rdf:type schema:DefinedTerm
122 sg:journal.1037502 schema:issn 1750-2799
123 1754-2189
124 schema:name Nature Protocols
125 rdf:type schema:Periodical
126 sg:person.01041560021.04 schema:affiliation https://www.grid.ac/institutes/grid.417743.2
127 schema:familyName Hiei
128 schema:givenName Yukoh
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041560021.04
130 rdf:type schema:Person
131 sg:person.01207707662.42 schema:affiliation https://www.grid.ac/institutes/grid.417743.2
132 schema:familyName Komari
133 schema:givenName Toshihiko
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207707662.42
135 rdf:type schema:Person
136 sg:pub.10.1007/bf00267709 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035059284
137 https://doi.org/10.1007/bf00267709
138 rdf:type schema:CreativeWork
139 sg:pub.10.1007/bf00290078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051467224
140 https://doi.org/10.1007/bf00290078
141 rdf:type schema:CreativeWork
142 sg:pub.10.1007/bf01977351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235724
143 https://doi.org/10.1007/bf01977351
144 rdf:type schema:CreativeWork
145 sg:pub.10.1007/bf02772592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048222118
146 https://doi.org/10.1007/bf02772592
147 rdf:type schema:CreativeWork
148 sg:pub.10.1007/bf02772648 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010639497
149 https://doi.org/10.1007/bf02772648
150 rdf:type schema:CreativeWork
151 sg:pub.10.1007/bf03030297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044288803
152 https://doi.org/10.1007/bf03030297
153 rdf:type schema:CreativeWork
154 sg:pub.10.1007/s00299-004-0843-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037588278
155 https://doi.org/10.1007/s00299-004-0843-6
156 rdf:type schema:CreativeWork
157 sg:pub.10.1007/s002990050625 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042646539
158 https://doi.org/10.1007/s002990050625
159 rdf:type schema:CreativeWork
160 sg:pub.10.1007/s11240-004-6154-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022186587
161 https://doi.org/10.1007/s11240-004-6154-3
162 rdf:type schema:CreativeWork
163 sg:pub.10.1007/s11240-005-9069-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007858377
164 https://doi.org/10.1007/s11240-005-9069-8
165 rdf:type schema:CreativeWork
166 sg:pub.10.1007/s11240-006-9157-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013520828
167 https://doi.org/10.1007/s11240-006-9157-4
168 rdf:type schema:CreativeWork
169 sg:pub.10.1023/a:1006307527449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019437708
170 https://doi.org/10.1023/a:1006307527449
171 rdf:type schema:CreativeWork
172 sg:pub.10.1023/a:1009661014167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004195512
173 https://doi.org/10.1023/a:1009661014167
174 rdf:type schema:CreativeWork
175 sg:pub.10.1023/a:1022393629691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024311747
176 https://doi.org/10.1023/a:1022393629691
177 rdf:type schema:CreativeWork
178 sg:pub.10.1023/b:biop.0000024271.38723.a6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049281360
179 https://doi.org/10.1023/b:biop.0000024271.38723.a6
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/338274a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040743981
182 https://doi.org/10.1038/338274a0
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/nbt0884-702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033876966
185 https://doi.org/10.1038/nbt0884-702
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/nbt0890-736 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010038336
188 https://doi.org/10.1038/nbt0890-736
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/nbt0988-1072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032752526
191 https://doi.org/10.1038/nbt0988-1072
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/nbt1091-957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014438272
194 https://doi.org/10.1038/nbt1091-957
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/sj.cr.149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026558025
197 https://doi.org/10.1038/sj.cr.149
198 rdf:type schema:CreativeWork
199 sg:pub.10.1385/1-59745-130-4:213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032232480
200 https://doi.org/10.1385/1-59745-130-4:213
201 rdf:type schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1077299600 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1077299601 schema:CreativeWork
204 https://doi.org/10.1016/0014-4827(68)90403-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027478679
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0168-9452(85)90086-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013553608
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.plantsci.2005.09.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032445032
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1046/j.1365-313x.1994.6020271.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023112743
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1073/pnas.71.9.3672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028573273
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1093/oxfordjournals.pcp.a077982 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083493326
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1111/j.1365-313x.2006.02836.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040941373
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1128/jb.166.1.88-94.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062714858
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1271/bbb.68.1193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007861462
223 rdf:type schema:CreativeWork
224 https://www.grid.ac/institutes/grid.417743.2 schema:alternateName Japan Tobacco (Japan)
225 schema:name Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan.
226 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...