A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-05

AUTHORS

Elizabeth S Ng, Richard Davis, Edouard G Stanley, Andrew G Elefanty

ABSTRACT

In order to promote the uniform and reproducible differentiation of human embryonic stem cells (HESCs) in response to exogenously added growth factors, we have developed a method (spin embryoid bodies (EBs)) that uses a recombinant protein-based, animal product-free medium in which HESCs are aggregated by centrifugation to form EBs. In this protocol we describe the formulation of this medium, denoted APEL (Albumin Polyvinylalcohol Essential Lipids), and its use in spin EB differentiation of HESCs. We also describe a more economical variant, BPEL (Bovine Serum Albumin (BSA) Polyvinylalchohol Essential Lipids), in which BSA replaces the recombinant human albumin. The integration of a medium that includes only defined and recombinant components with a defined number of cells to initiate EB formation results in a generally applicable, robust platform for growth factor-directed HESC differentiation. More... »

PAGES

768-776

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2008.42

DOI

http://dx.doi.org/10.1038/nprot.2008.42

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000342535

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18451785


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