Induction of pluripotent stem cells from fibroblast cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-12

AUTHORS

Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa, Shinya Yamanaka

ABSTRACT

Clinical application of embryonic stem (ES) cells faces difficulties regarding use of embryos, as well as tissue rejection after implantation. One way to circumvent these issues is to generate pluripotent stem cells directly from somatic cells. Somatic cells can be reprogrammed to an embryonic-like state by the injection of a nucleus into an enucleated oocyte or by fusion with ES cells. However, little is known about the mechanisms underlying these processes. We have recently shown that the combination of four transcription factors can generate ES-like pluripotent stem cells directly from mouse fibroblast cultures. The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells. More... »

PAGES

3081-3089

References to SciGraph publications

Journal

TITLE

Nature Protocols

ISSUE

12

VOLUME

2

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2007.418

    DOI

    http://dx.doi.org/10.1038/nprot.2007.418

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003600573

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18079707


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