Silver staining DNA in polyacrylamide gels View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11

AUTHORS

Brant J Bassam, Peter M Gresshoff

ABSTRACT

This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis (PAGE). Sensitivity rivals radioisotopic methods and DNA in the picogram range can be reliably detected. The described protocol is fast (approximately 1 h) and is implemented using readily available chemicals and materials. To achieve the sensitivity and visual clarity expected, quality reagents and clean handling are important. The updated protocol described here is based on the widely used method of Bassam et al. (1991), but provides improved image contrast and less risk of staining artefacts. More... »

PAGES

2649-2654

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2007.330

DOI

http://dx.doi.org/10.1038/nprot.2007.330

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046210582

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18007600


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