Agrobacterium-mediated transformation of maize View Full Text


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Article Info

DATE

2007-07

AUTHORS

Yuji Ishida, Yukoh Hiei, Toshihiko Komari

ABSTRACT

Maize may be transformed very efficiently using Agrobacterium tumefaciens-mediated methods. The most critical factor in the transformation protocol is the co-cultivation of healthy immature embryos of the correct developmental stage with A. tumefaciens; the embryos should be collected only from vigorous plants grown in well-conditioned glasshouses. With the protocol described here, approximately 50% of immature embryos from the inbred line A188 and 15% from inbred lines A634, H99 and W117 will produce transformants. About half of the transformed plants are expected to carry one or two copies of the transgenes, which are inherited by the progeny in a mendelian fashion. More than 90% of transformants are expected to be normal in morphology. The protocol takes about 3 months from the start of co-cultivation to the planting of transformants into pots. More... »

PAGES

1614-1621

References to SciGraph publications

  • 2006-06. Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1990-09. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants in BIO/TECHNOLOGY
  • 2006-08. High-Level Expression of Cold-Tolerant Pyruvate, Orthophosphate Dikinase from a Genomic Clone with Site-Directed Mutations in Transgenic Maize in MOLECULAR BREEDING
  • 2000-07. The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants (Zea mays L.) via Agrobacterium transformation in PLANT CELL REPORTS
  • 1996-06. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens in NATURE BIOTECHNOLOGY
  • 1993-02. Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis in NATURE BIOTECHNOLOGY
  • 2004-02. Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation in MOLECULAR BREEDING
  • 2000-12. Agrobacterium-mediated sorghum transformation in PLANT MOLECULAR BIOLOGY
  • 2002-12. High throughput genetic transformation mediated by Agrobacterium tumefaciens in maize in MOLECULAR BREEDING
  • 1994-09. Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment in PLANT MOLECULAR BIOLOGY
  • 2006-12. Improved frequency of transformation in rice and maize by treatment of immature embryos with centrifugation and heat prior to infection with Agrobacterium tumefaciens in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1985-09. A chimaeric hygromycin resistance gene as a selectable marker in plant cells in PLANT MOLECULAR BIOLOGY
  • 2006-10. Improved Agrobacterium-mediated transformation of three maize inbred lines using MS salts in PLANT CELL REPORTS
  • 2005-11. Successful Agrobacterium-Mediated Genetic Transformation of Maize Elite Inbred lines in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1997-09. A Rapid and Simple Method of Assaying Plants Transformed with Hygromycin or PPT Resistance Genes in PLANT MOLECULAR BIOLOGY REPORTER
  • 2002-08. High Efficiency Transgene Segregation in Co-Transformed Maize Plants using an Agrobacterium Tumefaciens 2 T-DNA Binary System in TRANSGENIC RESEARCH
  • 2004-10. Expression of cold-tolerant pyruvate, orthophosphate dikinase cDNA, and heterotetramer formation in transgenic maize plants in TRANSGENIC RESEARCH
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • Journal

    TITLE

    Nature Protocols

    ISSUE

    7

    VOLUME

    2

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nprot.2007.241

    DOI

    http://dx.doi.org/10.1038/nprot.2007.241

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011408003

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17585302


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