A general purpose method for extracting RNA from Dictyostelium cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-06

AUTHORS

Karen E Pilcher, Pascale Gaudet, Petra Fey, Anthony S Kowal, Rex L Chisholm

ABSTRACT

Here we present a protocol for the extraction of RNA from Dictyostelium discoideum. Dictyostelium is a social amoeba that undergoes a basic developmental program, and therefore analysis of RNA levels over a time course is a commonly used technique. This procedure is similar to other guanidine thiocyanate-based methods; however, it has been adjusted because of the large quantities of carbohydrate and nucleases found in Dictyostelium cells. After cell lysis and phenol:chloroform extraction, the resulting high-quality RNA isolated with the described protocol allows the molecular genetic analysis of wild-type and genetically modified cells. The purified RNA can be used for analyses such as northern blotting, RT-PCR and microarrays. This procedure requires approximately 2 h to complete. More... »

PAGES

1329-1332

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2007.191

DOI

http://dx.doi.org/10.1038/nprot.2007.191

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037199904

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17545970


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