Agrobacterium rhizogenes-mediated transformation of soybean to study root biology View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-04

AUTHORS

Attila Kereszt, Dongxue Li, Arief Indrasumunar, Cuc DT Nguyen, Sureeporn Nontachaiyapoom, Mark Kinkema, Peter M Gresshoff

ABSTRACT

This protocol is used to induce transgenic roots on soybean to study the function of genes required in biological processes of the root. Young seedlings with unfolded cotyledons are infected at the cotyledonary node and/or hypocotyl with Agrobacterium rhizogenes carrying the gene construct to be tested and the infection sites are kept in an environment of high humidity. When the emerged hairy roots can support the plants, the main roots are removed and the transgenic roots can be tested. Using this method, almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments. More... »

PAGES

948-952

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2007.141

DOI

http://dx.doi.org/10.1038/nprot.2007.141

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021137406

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17446894


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