Ontology type: schema:ScholarlyArticle Open Access: True
2007-05
AUTHORSJinyong Luo, Zhong-Liang Deng, Xiaoji Luo, Ni Tang, Wen-Xin Song, Jin Chen, Katie A Sharff, Hue H Luu, Rex C Haydon, Kenneth W Kinzler, Bert Vogelstein, Tong-Chuan He
ABSTRACTRecombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We have developed an approach that simplifies the generation and production of such viruses called the AdEasy system. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eukaryotic cells. After transfection of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of GFP encoded by a gene incorporated into the viral backbone. This system has expedited the process of generating and testing recombinant adenoviruses for a variety of purposes. In this protocol, we describe the practical aspects of using the AdEasy system for generating recombinant adenoviruses. The full protocol usually takes 4-5 weeks to complete. More... »
PAGES1236-1247
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DOIhttp://dx.doi.org/10.1038/nprot.2007.135
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