Laser-capture microdissection View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-08

AUTHORS

Virginia Espina, Julia D Wulfkuhle, Valerie S Calvert, Amy VanMeter, Weidong Zhou, George Coukos, David H Geho, Emanuel F Petricoin, Lance A Liotta

ABSTRACT

Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, RNA transcript profiling, cDNA library generation, proteomics discovery and signal-pathway profiling. Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1-1.5 h. More... »

PAGES

586-603

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2006.85

DOI

http://dx.doi.org/10.1038/nprot.2006.85

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012789273

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17406286


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