Cell culture and infection system for hepatitis C virus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-12

AUTHORS

Takanobu Kato, Tomoko Date, Asako Murayama, Kenichi Morikawa, Daisuke Akazawa, Takaji Wakita

ABSTRACT

Hepatitis C virus (HCV) infection causes chronic liver disease and is a worldwide health problem. Despite ever-increasing demand for knowledge on viral replication and pathogenesis, detailed analysis has been hampered by a lack of efficient viral culture systems. We isolated HCV genotype 2a strain JFH-1 from a patient with fulminant hepatitis. This strain replicates efficiently in Huh7 cells. Efficient replication and secretion of recombinant viral particles can be obtained in cell culture by transfection of in vitro-transcribed full-length JFH-1 RNA into Huh7 cells. JFH-1 virus generated in cell culture is infectious for both naive Huh7 cells and chimpanzees. The efficiency of viral production and infectivity of generated virus is substantially improved with permissive cell lines. This protocol describes how to use this system, which provides a powerful tool for studying viral life cycle and for the construction of antiviral strategies and the development of effective vaccines. Viral particles can be obtained in 12 days with this protocol. More... »

PAGES

2334-2339

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2006.395

DOI

http://dx.doi.org/10.1038/nprot.2006.395

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011164512

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17406476


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