Quantification of starch in plant tissues View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Alison M Smith, Samuel C Zeeman

ABSTRACT

This protocol describes a simple means of measuring the starch content of plant tissues by solubilizing the starch, converting it quantitatively to glucose and assaying the glucose. Plant tissue must initially be frozen rapidly to stop metabolism, then extracted to remove free glucose. Starch is solubilized by heating, then digested to glucose by adding glucan hydrolases. Glucose is assayed enzymatically. The method is more sensitive and accurate than iodine-based protocols, and is suitable for tissues that have a wide range of starch contents. Measurements on multiple samples can be completed within a day. More... »

PAGES

1342

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2006.232

DOI

http://dx.doi.org/10.1038/nprot.2006.232

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041567358

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17406420


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